犬淋巴细胞转化增殖试验最佳条件的确定

本试验选择杂种牧羊犬作为实验犬,用MTT法检探讨了不同浓度犊牛血清（FCS）、淋巴细胞、植物血凝素P（PHA-P）和刀豆蛋白A（ConA）对犬淋巴细胞增殖的影响。确定了犊牛血清含量为10%,淋巴细胞浓度为5×106个/mL,用丝裂原PHA-P 刺激的最佳浓度为12.5ug/mL时,MTT法测定能够刺激犬淋巴细胞转化增殖,但t检验分析表明其促进淋巴细胞增殖的效果并不显著;当其它条件不变,所用的丝裂原为Con A时,它在浓度为0.5～2.5μg/mL范围内均可以显著刺激T细胞的增殖（P<0.05）,且在浓度为2.5μg/mL时刺激效果最佳     

葡萄糖酸钙注射液的细菌内毒素检查

目的：建立葡萄糖酸钙注射液的细菌内毒素检查方法。方法：根据2005年版《中国兽药典》一部附录细菌内毒素检查法进行试验。结果：当水为溶剂时,供试品对细菌内毒素检查法有干扰作用;改用稀释剂I作为溶剂,干扰预试验和干扰试验表明,供试品在2倍以上稀释浓度时可排除干扰。结论：葡萄糖酸钙注射液按限值L＝2.5Eu/mL进行细菌内毒素检查是可行的,可以用细菌内毒素检查法代替热原法检测葡萄糖酸钙注射液。     

Remote Test Method of Relay Protection

Considering the limitation of the field test for relay protection in power system, the paper studies and discusses the remote test method of relay protection in power system. It provides the technology requirements, the technology problems and restricted factors to build the protection test and analysis center. It proposes the method of building the protection test and analysis center. Considering the practice for the transmission of test data, the paper introduces the technology requirements of utilizing dispatching automation system data network to transmit the protection test data. Considering the requirements of microcomputer protection devices for remote test method, the paper presents that there are remote test function and synchronization test function in the field microcomputer protection devices.     

Test Bench of Air Braking System for Vehicles

The stability of the brake system is a key factor for the vehicle safety.It is important for the air braking system's stability to shorten the time lag between the front brake and the rear brake,the reasonable disposal of the air pipeline and the optimization of the braking system design.A test bench is developed to test the performance of the air braking system,which is mainly made of pressure sensors,signal amplifiers,data acquisition system,computer,pressing air resource and so on.Based on this test bench,the reaction time of the system while braking and abandoning brake can be measured.Using this test bench,the performances of the air braking system of a certain commercial vehicle is detected.Compared with the actual road test,it can save the cost and the time for experiment largely.     

Flexible Field Calibration Solution of Environmental Test Equipment

In accordance with the shortage on the application of the traditional calibration Solution of environmental test equipment, this paper proposes a solution of flexible field calibration , which can be applicable to all sorts of environmental test equipments widely. The frame of system hardware and software is presented firstly through the system requirement analysis, then the key matter - - flexible calibration is analyzed and expatiated more deeply. Through actual test , it is shown that the solution put forward in this paper have notable applied values and spread significances.     

茄科蔬菜种子活力测定的研究

本试验选择茄科的茄子、番茄和辣椒各3个品种，于1993年—1995年进行了活力测定的研究。结果表明：控制劣变测定法、盆栽幼苗检验法和发芽测定法在测定茄科3种作物的种子活力水平、判断种子的耐藏性方面是行之有效的方法；四唑（TTC）定量法、电导率测定法也可测定3种作物种子的活力水平，但不能反映种子个体活力水平，只能反映种子群体的活力水平。此外，本试验总结出了茄科的茄子、番茄和辣椒种子在控制劣变测定种子活力时所要求的特定含水量，分别为22．0％、21．0％和19．0％。     

扇形试验设计在检测农作物种植密度的应用研究

为了检验扇形试验设计用于检测农作物种植密度的可行性,确定蚕豆新品种‘通蚕鲜8号’在重庆地区的最佳种植密度。应用扇形试验设计方法,经2年重复试验,研究结果表明应用扇形试验设计检验蚕豆种植密度是可行的。‘通蚕鲜8号’在重庆最佳种植密度范围在86805~106110株/hm2内,其中种植密度为93615株/hm2时产量达最高水平。     

Abnormal Noise Test Method of Vehicle Hydraulic Shock Absorber

The work principle and abnormal noise test method of hind vehicle hydraulic shock absorber are analyzed, Based on experiment data and signal character analyse ,a test method is found to distinguish the abnormal noise of rear hydraulic shock absorber. Practical validates that the reliability rate of this test method can be over 98 percents, and it can be satisfied with the request of the factory. At last, the reason of abnormal noise is analyzed and simulated, the reason of different signal attenuation is explained, and the research conclusion is educed.     

当前普通玉米区域试验中有关问题的探讨

对玉米区域试验工作的重要性和当前中国玉米区域试验的基本设置和现状进行了介绍。同时,根据作者多年从事玉米育种和玉米区试工作的体会对当前中国玉米区域试验中存在的有关问题,如人为因素影响试验准确性、各种自然因素造成的试验点报废等问题进行了分析,并对相应的解决办法进行了探讨,希望能对中国玉米区域试验和玉米品种审定工作的健康发展提供有益的参考。     

Test Method of Indoor Noise for Micro-surfacing Mix

Micro-surfacing mix has been wildly used in the pavement maintenance for its water proofing, anti-skidding and wear resistance. However, micro-surfacing pavement has higher noise than the common asphalt pavement and its noise can not be measured effectively and accurately. Therefore, the indoor noise test method of micro-surfacing mix based on wet track abrasion test was explored. The micro-surfacing mix specimens were used to imitate the micro-surfacing pavement. The wet track abrasion tester was used to provide the vehicle loads. The sound level meter was used to measure the noise between tire and pavement. Furthermore, the indoor noise of nine kinds of micro-surfacing mixes with different gradation was examined. The results show that the equivalent sound level in the second time period has good relationship with the surface texture depth by sad patch method. Then the test method and test procedure for the indoor noise of micro-surfacing mix based on wet track abrasion test were given. Finally, two micro-surfacing mixtures with different gradations were made to validate the test method. The results show that the evaluation method can effectively distinguish the difference of micro-surfacing mixes with different gradation and can supplement the mix design method of micro-surfacing mixes.     

蓖麻杂交种品种比较试验初报

为了鉴定和筛选最近培育成功的蓖麻优良品种,我们按照杂交蓖麻区域试验的标准要求,采取三次重复,随即排列的方法,进行了品种比较试验,通过试验表明淄蓖麻7号、5号和6号产量分别达到330.13kg/667㎡、320.10kg/667㎡和305.24kg/667㎡,分别比对照淄蓖麻2号增产59.43%、54.59%和47.41%,各品种生育期适中,综合性状优良,适宜我国蓖麻产区引种推广。     

种子活力光学无损检测技术研究进展

种子活力检测是种子检测中的重要一环,国际种子检验协会建议的检测方法主要有标准发芽实验法、TTC染色法、电导率测定和加速老化试验等方法。这些检测方法具有检测结果显示直观、测量准确等优点,但也存在对种子有破坏作用、检测过程复杂和耗时耗力等缺点。随着现代免耕直播技术的发展,对种子活力的检测在速度和无损性方面提出了更高的要求,而传统方法难以实现快速无损检测目标。基于光学技术的种子活力检测方法具有效率高、无损的特点,近年来得到了国内外相关学者的关注。本文对光学技术在种子活力检测上的应用进行了综述,介绍了X光、近红外光谱、高光谱、光声光谱、可调谐半导体激光吸收光谱和色选技术等光学技术在种子活力检测中的研究,并对光学技术在种子活力检测的前景进行了展望。     

蜱传立克次体病检测方法研究进展

为了选择更适合蜱传立克次体检测方法。通过对已发现的几种蜱传立克次体病的简单介绍,并且对目前该病检测方法的概述、分类、优缺点对比。从而为发展新的检测方法提供思路,并对未来的研究方向做了展望。     

Analysis of the Lateral Deformation of Structural Soft Dredger Fill with True Triaxial Test

In order to study the mechanical properties and structural properties of soft dredger fill under lateral deformation. Based on true triaxial and WF stress path apparatus, lateral unloading undrained tests were carried out. The results derived from true triaxial test, WF stress path test and routine triaxial shear test were analyzed. The contrastive conclusions are as follows: The stress-strain curve of true triaxial unloading test shows a softening behavior, which is different from the hardening behavior for that of conventional triaxial shear test. With the increase of initial confined pressure, the dilatancy of soil transfers from shrinkage to expansion. The structural yield stress of the unloading soil with true triaxial test method is dramatically larger than that with the latter two tests due to the effect of intermediate principal, which exhibits a nonlinear growth as the coefficient of intermediate principal bd stress jumps. The shear strength indexes of unloading soil with true triaxial test is larger than that with WF stress path test. Compared with the results of routine triaxial test, the internal friction angle of true triaxial test is bigger while the cohesion is smaller.     

区域试验的多对照设置和品种的优度估计

以一组小麦区域试验资料为例,介绍了分析方法。1.一种基于对照和参试品种间的成对GE互作均方(MS_(GE))的分析。2.优度估计。品种×环境资料的品种总优度值定义为全部环境中平均的品种响应值与最大响应值的距离均方。这个分析可以给予育种者选择地区适应的品种作为对照以较大的灵活性。     

GGE双标图对抗寒烤烟品种区域试验的分析

利用2012年南平市抗寒烤烟品种区域试验中各品种在3个不同试验点上的平均产量和平均产值,经过环境中心化后的数据,以GGE双标图的图谱形式阐述了参试品种在各试验点的表现、各试验点环境间关系、各试验点的区分力和代表性及各参试品种平均表现和稳定性。结果表明：（1）‘CB-1’在延平试验点平均产量、平均产值最高,‘NF-1’在邵武、浦城试验点平均产值最高;（2）邵武和浦城试验点之间存在紧密正相关,且与延平试验点均表现为负相关;（3）延平、浦城、邵武各试验点对参试品种均具有较强的区分能力,作为抗寒品种筛选的试验点,浦城和邵武具有较强代表性;（4）5个参试品种中‘NF-2’表现既高产量、高产值又稳定的品系,‘NF-1’表现相对高产和稳定的品系。     

浙江省西部地区双低油菜品比试验

选用多个双低油菜品种在浙江省西部山区进行试种，筛选出适合本生态区种植的优质、高产双低油菜品种。试验结果表明：杂2005-1生育期适中，产量高，可参加下一年度省级区试。湘杂油3号、沪油杂1号，丰产性好，可分别进一步试验示范和参加下一年度区试。     

Separated Teeth Gear for Grinding Test

A kind of rational structure gear,separated teeth gear,in applied to grinding test.The contadiction between the heavy weight of the tested gear and the high precision of the measuring instrument is ideally solved.The gear is tested and verified by experiments.     

The Acute Toxicity and Subchronic Toxicity Test of Zhuqin Extract

[Objective] To investigate the safety of compound Zhuqin extract.[Methods]In acute toxicity test,mice in test group were given compound Zhuqin extract( i. g.) by gavage,the dosage of which was 0. 8,0. 6,0. 45,0. 35 g /20 g,to determine the median lethal dose of compound Zhuqin extract( LD50). In maximum tolerance experiment,mice in test group were given compound Zhuqin extract( i. g.) by gavage,the dosage of which was 0. 5 g /20 g,to determine the maximum tolerance dose( MTD); In the sub- chronic toxicity test,rats in test group were given compound Zhuqin extract( i. g.) by gavage for 30 d,the dosage of which was 2. 0,1. 5,1. 0 g /200 g,and normal saline negative control group was set. Clinic condition of rat was observed everyday,hematology,blood biochemistry,anatomy,organ index and organization pathological observation were conducted after test.[Results]The acute toxicity test failed to detect median lethal dose of compound Zhuqin extract; the weight differences were not significant( P 0. 05); some blood biochemical criterion and blood routine index had significant differences( 0. 01 P 0. 05),and some indexes had extremely differences( P 0. 01); organ index,necropsy and histopathology had abnormalities.[Conclusions] Compound Zhuqin extract was safe and reliable.     

甜荞品种比较试验初报

2012年在赤峰市进行9个甜荞品种比较试验。结果表明：榆杂荞-2和赤荞1号产量最高,可在赤峰地区推广种植,通辽本地大粒甜荞、定甜2号、榆荞4号、甜0103-3、丰甜1号、通辽本地小粒甜荞产量性状较差,不适宜当地推广种植。