Putative Identification and Adduct Formation of Reference Materials for Cotton Secondary Metabolite using UPLC-ESI-MS Analysis

[Objective] The aim of this study is to establish an method for cotton metabolites identification by ultra-performance liquid chromatography-electrospray ionization-mass spectrometry (UPLC-ESI-MS) analysis, and to investigate the adduct types, dominant adducts and appropriate ESI ion modes of cotton secondary metabolites under the determined UPLC and ESI conditions. [Method] UPLC-ESI-MS was employed to analyze 18 cotton metabolite standards, Online XCMS software was used to extract the nontargeted mass spectrum data, and MATLAB software was used to prepare calculation programs for the identification method of cotton metabolite standards. [Result] A high-throughput identification method of cotton secondary metabolite, named POSid and NEGid separately for the positive and negative ESI modes, was established based on the calculated accurate molecular weight. In the determined UPLC condition and positive and negative ESI mode, 14 cotton metabolite standards were correctly identified. There found 6 adducts including [M+H]⁺, [M+Na]⁺, [M+NH₄]⁺, [2M+NH₄]⁺, [2M+Na]⁺ and [2M+H]⁺ in positive ion mode, and 8 adducts including [M-H]^-, [2M-H]^-, [M+Cl]^-, [M+FA-H]^-, [3M-H]^-, [M+Na-2H]-, [M-H₂O-H]^- and [M+TFA-H]^- in negative ion mode, while 1 to 6 adducts were observed in the mass spectrum of a single standard, and each metabolite standard had a dominant adduct of a preference for ESI mode. Melibose was suitable for ESI positive ion mode detection, gossypol was suitable for both ion modes detection; and 12 compounds were suitable for negative ion mode detection due to mass spectrum signals of their dominant adduct stronger in negative ion mode than positive ion mode. [Conclusion] Based on the accurate molecular weight, the established identifying method can identify the 18 cotton metabolite standards with their nontarget mass spectrometry data. Under the determined UPLC and ESI condition, the dominant adducts of cotton secondary metabolites have the preference of ESI mode. These results provide technical and theoretical data support for further study of cotton metabolomics.     

凋萎时间对青玉米秸秆青贮饲料营养成分的影响

【研究目的】青贮饲料调制是一项短时间内的突击性工作，刈割青玉米秸秆未能尽快调制青贮致使青贮饲料质量受影响；【方法】为此本试验选用农大108玉米秸秆收获后凋萎的1d、3d、5d、7d调制青贮，以研究刈割后凋萎时间对青贮饲料营养成分的影响；【结果】在本试验的凋萎条件下，试验期内玉米秸秆中干基水溶性碳水化合物含量、水分含量逐渐下降，凋萎7d的水溶性碳水化合物、水分含量显著低于其它处理组，在凋萎的1d、3d、5d、7d调制青贮，凋萎7d的青贮pH和氨态氮比例显著高于凋萎1d(pH,4.82比5.27；氨态氮/总氮,5.90%比8.56%)，但青贮饲料中乳酸、粗蛋白、中性洗涤纤维和酸性洗涤纤维含量没有显著差异。【结论】结果表明收刈后青玉米秸秆5d之内可以调制青贮     

紫外线诱变选育高产纳豆激酶菌株的研究

为了获得一株产纳豆激酶(NK)活力较强的菌株。以纳豆激酶活力为评价指标,采用紫外线诱变育种的方法和单因素实验方法,确定紫外线诱变剂量,对原始菌株进行诱变育种,并采用纤维蛋白平板法,测定菌株发酵的纳豆激酶活力。试验结果表明,20W紫外灯距离30cm照射240s的诱变,效果较佳。经过诱变及菌种筛选所获得的高产菌株U-5发酵生产的纳豆激酶活力比原始菌株T-3提高了8.81%。经15代传代试验,U-5高效突变株生产的纳豆激酶活力均稳定在5440.37IU/mL以上。     

收刈时间对玉米秸秆营养物质产量的影响

【目的】从玉米植株秸秆的总可消化养分产量考虑，确定植株的适宜收刈时间可有效利用秸秆饲料资源，【方法】为此本试验大田试验种植4个早中熟玉米品种，包括农大108、高油298、冀丰58、郑单958，在生长期的92d、96d、100d和104d收刈秸秆，研究收刈时间对秸秆营养物质产量的影响，【结果】试验结果表明，收刈时间明显影响玉米秸秆的有机物、蛋白质、纤维性物质和可消化有机物产量，【结论】所选种的玉米品种秸秆蛋白质和可消化有机物产量在100d达到峰值     

Optimization of Extraction Method of Triterpenoids from Artemisia selengnesis Turcz

[Objectives]The research aimed to optimize the extraction method of triterpenoids from A. selengnesis. [Methods]The ultrasonic extraction of triterpenoids in A....     

培养基中玉米秸秆用量对鸡腿蘑生育的影响

【目的】研究玉米秸秆对鸡腿蘑发育的影响,为合理利用秸秆资料提供有效途径;【方法】设四个不同秸秆用量的培养基配方,通过单因子试验分析秸秆对鸡腿蘑菌丝长速与产量的影响;【结果】各处理在发菌时间,出菇时间,菇产量以及子实体形态等方面均有明显差异,其中玉米秸秆含量为35%的配方菌丝长势旺,生长快,菇体生物学效率最高;【结论】鸡腿蘑可以利用玉米秸秆中营养物质,秸秆中添加适量的腐叶土栽培鸡腿蘑是可行的。     

Effects of Paeonol on Expression of Type Ⅰ and Type Ⅲ Collagen of High Glucose Induced Proliferation of Cardiac Fibroblasts

[Objectives]To explore the effects of paeonol on the inhibition of myocardial fibrosis in high glucose induced cardiac fibroblasts(CFs).[Methods]Differential ad...     

淫羊藿苷对鸡胚成骨细胞增殖和分化的影响

【研究目的】本文研究了淫羊藿苷对鸡胚成骨细胞增殖和分化的影响。【方法】将不同浓度的淫羊藿苷加入第二代成骨细胞中,分别于培养24、48、72h时,使用MTT法和PNPP法检测成骨细胞的增殖率和碱性磷酸酶(ALP)活性。【结果】淫羊藿苷促进鸡胚成骨细胞增殖和分化,但增殖作用不显著。其中当淫羊藿苷浓度为20ng/ml时,对成骨细胞增殖分化作用最强。【结论】淫羊藿苷能够显著促进鸡胚成骨细胞分化,对成骨细胞增殖有一定作用。     

Frequencies of plasmon types in a germ plasm sample of peanuts,Arachis hypogaea

Summary The plasmon constitution of 68 different accessions of cultivated peanuts (Arachis hypogaea L.) was studied in crosses with one or another of three testers: V4, VSM and HG1, having the [V4], [O] and [G] plasmons, respectively. The plasmons interact differently with three plasmon-sensitive nuclear genes, thereby determining whether the plants will be erect or trailing. From the phenotypes of the reciprocal F₁ hybrids it was concluded that the [G] plasmon of HG1 is rare-it is present in this cultivar and possibly in a few other; the [V4] plasmon is rare, being present in the V4 cultivar and possibly in a few other accessions; the [O] plasmon is widespread, being present in lines from various geographical origins and in at least three of the four botanical types of cultivated peanuts.     

Study on Quality Standards of Children Antipyretic Lotion

[Objectives]To establish suitable quality standards of children antipyretic lotion.[Methods]Qualitative identification was performed for Paederia scandens and A...     

Effects of Different Storage Methods and Storage Time on the Quality of Codonopsis pilosula

[Objectives]To study the effects of different storage methods and storage time on the quality of Codonopsis pilosula.[Methods]Experiment was performed by settin...     

棉花非叶器官的叶绿素荧光异质性研究

【目的】棉花叶片和非叶器官的结构和功能具有异质性,本文针对这一问题通过叶绿素荧光成像技术来研究叶片和非叶器官的光合异质性。【方法】本试验以陆地棉品种新陆早45号为材料,测定了叶绿素含量和叶绿素荧光参数等,以此探讨棉花叶片和非叶器官的光合特性和异质性。【结果】试验结果表明,棉花叶片及非叶器官均存在光合功能的异质性。通过比较不同器官的异质性表明,与叶片相比,非叶器官光合功能的变异程度较大;且不同参数的异质性随光照强度而变化。无论叶片还是非叶器官,与光化学过程相关的参数均随光照强度增加异质性变大,而与非光化学过程相关的参数均表现出随光照强度增加异质性变小的特点。【结论】在研究过程中,如使用普通光纤式叶绿素荧光仪来测定样品(叶片等)的光合性能时,需要对样品的不同部位进行多次测定,才能更好地反映所测定组织的光合性能。而如需要较精确的光化学猝灭或者非光化学猝灭相关的参数时,无论叶片还是非叶器官均需要加大测定的重复数来进行分析,以达到更为准确的目的。     

毛头鬼伞多糖抗烟草花叶病毒（TMV）活性研究初报

【目的】为探讨毛头鬼伞多糖（Coprinus comatus）的生物活性,对毛头鬼伞多糖抗烟草花叶病毒(Tobacco mosaic virus,TMV)活性进行测定,明确活性部位,为进一步分离纯化提供理论依据。【方法】从毛头鬼伞子实体和菌丝体提取多糖,并初步纯化,采用半叶法和叶碟法对抗病毒活性进行测定。【结果】毛头鬼伞多糖对TMV具有较强的体外抑制和抗病毒侵染作用,对TMV具有明显的体内抑制复制效果;在TMV接种前施用可以显著降低TMV侵染能力。【结论】毛头鬼伞多糖具有较好的抗TMV活性,为其进一步开发利用提供理论依据。     

Establishment of Specific Chromatogram of Spica Prunellae Formula Granules Based on Standard Decoction

[Objectives]This study aimed to establish HPLC chromatograms of decoction pieces,standard decoction and formula granules of Spica Prunellae.[Methods]The chromat...     

GC-MS法检测广藿香挥发油的化学成分

摘要 [目的] 建立广藿香挥发油的指纹图谱检测方法,对广藿香的品质进行控制。[方法] 采用GC-MS法对广藿香挥发油成分进行定性和量化分析,并对市售的不同批次样品进行检测。[结果] 以9个共有峰为评价指标,实验的重复性、稳定性、重现性良好;不同批次的样品各组分的含量有显著差异。[结论] 以9个共有峰作为广藿香挥发油的指纹图谱,以广藿香酮与广藿香醇的峰面积比值作为控制质量的指标参数,建立了相应的指纹图谱。建议在使用广藿香时应根据产品的不同特点建立相应的指纹图谱质量标准。     

滤纸片法筛选不同活性物对棉花黄萎病菌抑制效果研究

[ 目的] 为了筛选对棉花黄萎病菌有效的活性物。[ 方法] 采用滤纸片测定了52种活性物对棉花黄萎病菌的抑制效果。试验结果的记载由分级记载法表示、直观图片表示和160倍显微图片表示。[ 结果] 结果表明,本研究筛选出了14种对棉花黄萎病菌抑制效果显著的活性物。[ 结论] 该研究为筛选对棉花黄萎病菌高效活性物及田间防治棉花黄萎病提供理论依据。     

Chemical Composition of Rubus pirifolius Smith

[Objectives]This paper aimed to study the chemical composition of Rubus pirifolius Smith[Methods]A preliminary experiment was carried out using the qualitative ...     

Inhibitiont of Obazema on Proliferation of Gastric Cancer BGC-823 Cells and Its Mechanism

[Objectives]This study aimed to investigate the inhibiting effect of Obazema on proliferation of gastric cancer BGC-823 cells and its mechanism.[Methods]BGC-823...     

Establishment of Fingerprint of Cryptolepis buchananii Roem. et Schult. by HPLC

[Objectives]This study aimed to establish the fingerprint of Cryptolepis buchananii Roem.et Schult.by HPLC.[Methods]Taking the peak of chlorogenic acid as the r...     

Determination of Naringin Content in Peel of Guangxi Citrus maxima (Burm.) Merr by HPLC

[Objectives]To establish a method for determining the naringin content in the peel of Guangxi Citrus maxima(Burm.)Merr.[Methods]The high performance liquid chro...