Nitrapyrin effect on corn N‐15 uptake efficiency,denitrification and nitrate leaching

Abstract

The effect of nitrapyrin on the fate of fertilizer nitrogen (N) applied to soil needs further investigation. Our objective was to determine the effect of nitrapyrin under two different leaching regimes on the fate of ammonium sulfate‐nitrogen [(NH₄)₂SO₄‐N] added to the soil, namely corn N uptake, denitrification, nitrate leaching and soil residual N. A Nunn sandy clay loam soil (fine, montmorillonitic, mesic, aridic, argiustoll), low in residual inorganic N was used. Nitrogen‐15 enriched (NH₄)₂SO₄ (5 atom% N‐15) was applied at five rates (0, 50, 100, 200, and 400 mg/kg), nitrapyrin at three rates 0, 1.3, and 2.6 μL/kg (0, 2.36, and 4.72 L/ha) and leaching at two rates (0 and 1000 mL over field capacity in two 500‐mL increments at 3 and 6 weeks after planting) in a complete factorial arrangement with three replications. Corn (Zea mays L.) seeds were planted in pots (2 kg soil/pot) and allowed to grow for 80 days in a greenhouse. The atom% N‐15 values were determined in plant tops, leachates and soil samples using a mass spectrometer. The results showed that N fertilizer increased dry matter production, plant N concentration, leaching of nitrates and denitrification significantly. The effect of nitrapyrin on yield was not statistically significant, but, it at a rate equivalent to 4.72 L/ha reduced denitrification and nitrate (NO₃) leaching and increased N uptake efficiency. Application of 4.72 L/ha of nitrapyrin versus control showed the following results respectively, N uptake: 46.3 versus 39.6%, denitrification: 26.3 versus 35.3% and NO₃ leaching: 2.7 vesus 6.7% of fertilizer N‐15. Nitrapyrin increased soil residual fertilizer N‐15 in organic matter and roots. The result of this study show that application of nitrapyrin at an adequate rate decreases denitrification and NO₃ leaching and increases N uptake efficiency.     

灌溉与施氮对留茬免耕春小麦氮素吸收和氮肥损失的影响

在甘肃省石羊河流域绿洲灌区,采用裂区设计大田试验,研究不同灌溉量（常规灌溉（327 mm）、节水20%灌溉（261 mm）、节水40%灌溉（196 mm））和施氮量（0,140,221,300 kg/hm^2）对留茬免耕春小麦植株吸氮量、收获期土壤硝态氮（NO3^--N）含量和氮肥损失的影响。结果表明,在留茬免耕农田中,灌溉量从196 mm增加到327 mm,小麦籽粒含氮量从1.55%增加到1.71%,植株吸氮量从134 kg/hm^2增加到190kg/hm^2。当施氮量超过221 kg/hm^2时,籽粒含氮量、秸秆含氮量、植株吸氮量不再显著增加。施氮300,221,140 kg/hm^2处理的植株吸氮量比不施氮处理的分别提高47%,37%和18%;在春小麦收获期,土壤表层（0-60 cm）NO3^--N含量随灌溉量增加而减少,随施氮量增加而增加,灌溉和施氮对60 cm以下土壤NO3^--N含量影响不明显。与不施氮处理相比,施氮300,221,140 kg/hm^2的氮肥损失分别为186,137,94 kg/hm^2。     

改善根系生长捕获深层土中NO3-N最优化免耕玉米氮肥利用率

Subsoil acidity restricts root growth and reduces crop yields in many parts of the world. More than half of the fertilizer nitrogen(N) applied in crop production is currently lost to the environment. This study aimed to investigate the effect of gypsum application on the efficiency of N fertilizer in no-till corn(Zea mays L.) production in southern Brazil. A field experiment examined the effects of surface-applied gypsum(0, 5, 10, and 15 Mg ha~(-1)) and top-dressed ammonium nitrate(NH_4NO_3)(60, 120, and 180 kg N ha~(-1)) on corn root length, N uptake, and grain yield. A greenhouse experiment was conducted using undisturbed soil columns collected from the field experiment site to evaluate NO_3-N leaching, N uptake, and root length with surface-applied gypsum(0 and 10 Mg ha~(-1)) and top-dressed NH_4NO_3(0 and 180 kg N ha~(-1)). Amelioration of subsoil acidity due to gypsum application increased corn root growth,N uptake, grain yield, and N use efficiency. Applying gypsum to the soil surface increased corn grain yield by 19%–38% and partial factor productivity of N(PFPN) by 27%–38%, depending on the N application rate. Results of the undisturbed soil column greenhouse experiment showed that improvement of N use efficiency by gypsum application was due to the higher N uptake from NO_3-N in the subsoil as a result of increased corn root length. Our results suggest that ameliorating subsoil acidity with gypsum in a no-till corn system could increase N use efficiency, improve grain yield, and reduce environmental risks due to NO_3-N leaching.     

外源氮在中、低肥力红壤中的转化与去向研究

采用盆栽15N示踪技术,研究了外源化肥氮和有机氮在中、低肥力红壤中的转化过程及其在土壤团聚体中的分配特征。结果表明:无论是低肥力还是中等肥力红壤上,化肥与有机肥配施都能显著增加土壤硝态氮、微生物生物量碳、氮及酶活性,且显著提高氮肥在土壤中的残留率,减少氮肥损失。相同施氮条件下,小麦生育期内中等肥力红壤上微生物生物量碳、氮及硝态氮含量分别相当于低肥力土壤的1.8、1.3和2.0倍。中等肥力红壤上尿素的施入对小麦生育期土壤硝态氮无影响,而低肥力红壤上施用尿素使土壤硝态氮含量提高了5.7倍。施肥可以显著提高低肥力红壤上小麦的地上部吸氮量,相当于不施肥的2.1~3.3倍,但对于中等肥力红壤,不同施氮条件对小麦的地上部吸氮量无明显影响。施入有机肥可以显著增加土壤各粒级团聚体中外源氮的残留量,是单施化肥的2.1~5.0倍。与单施化肥氮相比,有机氮或与有机肥配施的化肥氮优先进入到大团聚体中,而外源氮在微团聚体和粉粘粒中的残留与分配并无明显差异。本研究说明有机肥或化肥与有机肥配施能有效降低氮肥的损失,提高氮肥在土壤中的保存,特别是对于中等肥力的土壤,有机肥的配合施用对于后季作物的生长具有重要意义。     

Effect of straw management, tillage timing and timing of fertilizer nitrogen application on the crop utilization of fertilizer and soil nitrogen in an irrigated cereal rotation

In irrigated grain-growing soils on Canada's prairies, straw management can affect nitrogen (N) fertility and long-term soil organic matter reserves. We conducted a 2-year field experiment in southern Alberta, on a Dark Brown Chernozemic Lethbridge loam (Typic Boroll), to determine the effects of straw removal, tillage, and fertilizer timing on crop uptake of soil and fertilizer N. During the study (1991 and 1992), the crop was oat (Avena sativa L.) and wheat (Triticum aestivum L.), respectively, in an experiment that had been in a wheat-wheat-oat-wheat rotation since 1986. Five straw-tillage treatments were: straw-fall plow, straw-pring plow, no straw-fall plow, no straw-spring plow and no straw-direct seeding. Fertilizer N was applied in fall or spring. Ammonium nitrate (5 at.% ¹⁵N) was added at 100 kg N ha⁻¹ in fall 1990 or spring 1991. For oat (1991), plant N derived from soil was higher under fall plow than under spring plow, higher with tillage than direct seeding, and unaffected by straw removal. The plant N derived from fertilizer was not affected by straw removal in fall plow treatments, but under spring plow, it was higher with straw removal. The plant N derived from fertilizer showed a significant straw-tillage × fertilizer timing interaction; with fall incorporated straw, plant N derived from fertilizer was 44.0 kg N ha⁻¹ for spring-applied, and 30.6 kg N ha⁻¹ for fall-applied N, but in other straw-tillage treatments there was no effect of fertilizer timing. Cumulative fertilizer N recovery (plant + soil) over the 2 years averaged 64.2%, and was unaffected by straw-tillage treatment. Fertilizer N recovery, however, was less with fall-applied N (61.3%) than spring applied N (66.8%). At mid-season, fall plow treatments had higher soil inorganic N and inorganic N derived from fertilizer than spring plow treatments, apparently because of less immobilization. The fall plow treatments also retained higher inorganic N after harvest. Straw removal and fertilizer timing did not influence soil inorganic N and soil inorganic N derived from fertilizer. N removal in straw (16 kg N ha⁻¹ yr⁻¹) could deplete soil N in the long-term. Long-term effects of tillage timing on soil N will depend on the relative amount of N lost by leaching with fall plowing and that lost by denitrification under spring plowing. With direct seeding, crop yield and uptake of soil N was less, and N losses by denitrification could be greater. Application of N in spring, rather than fall, should enhance crop N uptake, reducing N losses and enhancing long-term soil organic N.     

Quantitative effects of soil depth and soil and fertilizer nitrogen on nitrogen uptake by tall fescue and switchgrass

Abstract

In semi‐arid regions, soil depth influences soil N uptake, but not ferilizer N uptake. How soil depth interacts with soil and fertilzer N to influence N uptake in humid regions is not known. The objective was to determine the relative importance of soil depth and soil and fertilizer N uptake, by forage grasses. Tall fescue (Festuca arundinacea Schreb.) and switchgrass (Panicum virgatum L.) were grown on soils of varying depths. Nitrogen rates are 0, 90, and 180 kgN/ha of ¹⁵N depleted (NH₄)SO₄ applied in a split application on fescue and in one aplication to switchgrass. Total N and fertilizer N uptake, were regressed against fertilizer N, variables related to soil depth (waterholding capacity (WC), water use (WU), water loss (WL), and total soil N (SN). Soil variables explained 28% of the accoutable variation in total N uptake by first cut fescue but only 10% by second cut fescue. Soil variables explained 11% of the accountable variation in fertilizer N uptake by first cut fescue and none by the seoond. Soil variables explained 40% of the accountable variation in the total N uptake, by switchgrass, but only 10% of the variation in the fertilizer N uptake. Only where soil depth was less than 90 cm did it have a significant effect on the fertilizer N uptake by first cut fescue. Soil depth had no significant effect on the uptake, of fertilizer N by second cut fescue or switchgrass.     

几种有机和无机氮肥对草莓生长及其氮素吸收分配影响的差异

通过不同生育时期植株各部位的氮素分析,研究了不同商品有机肥和有机无机复混肥对大田草莓(FragariaAnanassa.c.v.Dutch.)植株生长及氮素吸收分配的影响。结果表明,施用不同肥料品种均可促进植物氮素吸收,提高氮素积累速率,促进氮素向果实中分配。在施等量氮素养分条件下,草莓器官的氮素吸收状况对不同氮肥品种反应不一。尽管无机肥处理的氮素积累速率和地上部全氮含量较高,但施用商品有机肥较无机氮肥或有机无机复混肥更能促进草莓生长发育和草莓果实产量的增加,说明无机氮不宜作为草莓基肥一次性施用。结果还表明,施肥处理氮素的日均积累量平均为10.8.mg/plant,而不施肥处理仅为5.1.mg/plant。在果实采收末期,不同处理草莓各器官的氮素分配趋势为果实茎和叶柄叶片根系。施用纯有机肥(OFA和OFB),果实中吸收的氮素超过植株吸收总量的一半,分别占53.5%和51.7%,无机氮肥处理(UN)和有机无机复混肥处理(OIF)的果实氮素分配率分别只有46.1%和39.8%。     

Impact of integrated application of biochar and nitrogen fertilizers on maize growth and nitrogen recovery in alkaline calcareous soil

Biochar application has been considered as a rich source of carbon which helps to improve the physico-chemical properties and fertility of the soil. In Pakistan, excessive use of nitrogen fertilizer is considered a serious problem, so it is of vital importance to examine the effect of biochar on soil with varying doses of nitrogen fertilizer. We hypothesized that addition of biochar to an alkaline calcareous soil could improve not only soil quality and crop yield but also nitrogen use efficiency (NUE), reducing the loss of nitrogen (N) in the form of denitrification, ammonia volatilization, and nitrate leaching. A pot experiment was conducted under 2-factorial completely randomized design having three replications to evaluate the NUE in biochar amended calcareous soil. Biochar was applied at the rate of 0%, 1% and 2% (w/w) in pots filled with 17 kg of soil using various levels of N (0%, 50% and 100% of recommended dose) on maize (Zea mays L.). Several soil quality indicators, uptake, and yield of maize were monitored. Biochar application significantly decreased soil pH, increased water-holding capacity, total organic carbon, maize yield, stomatal conductance, and nitrogen uptake in plant. The results of the study indicated that addition of biochar could not only decrease the use of inorganic fertilizers by improving its quality and yield as in our case biochar at the rate of 1% and N at the rate of 50% provided optimum output minimizing the economic cost eventually.     

Nitrogen Dynamics and Losses in Direct‐Drilled Maize Systems

Abstract

The knowledge of nitrogen (N) losses in direct‐drilling agrosystems is essential to develop strategies to increase fertilizer efficiency and to minimize environmental damage. The objectives were i) to quantify the magnitude of N volatilization and leaching simultaneously as affected by different urea fertilization rates and ii) to evaluate the capacity of these specific plant–soil systems to act as a buffer to prevent nitrate leaching. Two experiments were conducted during 2001/02 and 2002/03 growing seasons in Alberti, Argentina. The crop was direct‐drilled maize and the soil a Typic Argiudoll. Ammonia losses, N uptake by crop at flowering and harvest, grain yield, N in previous crop residues, and soil nitrate content up to 2‐m depths were determined. Nitrogen availability, soil nitrate (NO₃)‐N up to 1 m plus fertilizer N, was linearly and highly associated with crop N uptake at flowering (R²=0.93, P<0.01) and at harvest (R²=0.852, P<0.01). Around 17.5% of fertilizer N was lost by volatilization in 10 days. The obtained values of residual nitrate N up to the 150‐cm depth were associated (R²=0.960, P<0.001) with those predicted by the nitrate leaching and economic analysis package (NLEAP) model. Maize in the direct‐drilling system was able to cycle N from the previous crop residues, N from soil organic matter, and N from fertilizers with few losses.     

木质素对尿素在土壤中的转化及生物利用的影响

采用田间试验方法,研究了木质素施入土壤后对尿素转化及冬小麦生物量、氮素营养的影响。结果表明,木质素能调控无机态氮释放,表现出在冬小麦返青期施用木质素的土壤无机态氮含量较低而拔节期较高的趋势。施用木质素后土壤无机态氮的动态变化情况,有利于促进冬小麦的氮素吸收,促进植株对氮素的吸收利用。木质素提高了冬小麦生物量最大积累速率,其中铵法木质素提高了29%,碱法木质素提高了13%。铵法木质素、碱法木质素处理与化肥相比,氮肥农学利用率、氮肥表观利用率、氮肥偏生产力、氮素吸收强度都有所提高,且铵法木质素比碱法木质素效果明显。     

Nitrogen Fractions in Arable Soils in Relation to Nitrogen Mineralization and Plant Uptake

Abstract

Nitrogen (N) as a major constituent of all plants is one of the most important nutrients. Minimizing input of mineral nitrogen fertilizer is needed to avoid harm to the environment. Optimal input of mineral nitrogen should take the nitrogen supply of the soil into account. Many different soil tests have been proposed for determining soil nitrogen availability. In this article we present a new approach that is based on the measurement of nitrate, ammonium, and dissolved organic nitrogen (DON) in a 0.01 M CaCl₂ soil extract. Eighteen agricultural soils, differing widely in the availability of nitrogen were used, fertilized and unfertilized. It is shown that the nitrogen uptake by maize plants (Zea Mays L.) in both “N‐fertilized” and “N‐unfertilized” soils as measured in a pot experiment can be described with a simple model using the measured nitrogen fractions in the extract. The main source of nitrogen uptake by the plants is the mineralized organic nitrogen during the growing period. It is shown that the initial measured DON fraction is a good indicator of the nitrogen mineralized during plant growth.     

Optimum Nitrogen Fertilization of Winter Wheat Based on Color Digital Camera Images

Abstract

Site‐specific nitrogen (N) fertilizer management based on soil N_min (soil mineral N) and the plant N status (sap nitrate analysis and chlorophyll meter (SPAD) reading test) has been shown to be effective in decreasing excessive N inputs for winter wheat in the North China Plain, but the multiple sampling of soil and plants in individual fields is too time‐consuming and costly for producers and farmers. In this study, a color digital camera was used to capture wheat canopy images at a specific growth stage to assess N needs. Treatments included a farmer's N treatment (typical farmer practice), an optimum N treatment (N application based on soil–plant testing), and four treatments without N (one to four cropping seasons without any N fertilizer input). Digital images were analyzed to get red, green, and blue color‐band intensities for each treatment. Normalized intensities of the red, green, and blue color bands were well correlated with soil N_min, SPAD readings, sap nitrate concentration, and total N concentration of winter wheat. This research indicated the potential of using a digital camera as a tool combined with an improved N_min method to make N fertilizer recommendations for larger fields.     

施氮量对土壤无机氮分布和微生物量氮含量及小麦产量的影响

在高肥力土壤条件下,研究了施氮量对土壤无机氮分布和微生物量氮含量及小麦产量的影响。结果表明,小麦生长期间,施氮处理0100.cm土层硝态氮积累量显著大于不施氮处理;当施氮量大于150.kg/hm2时,随施氮量增加,0100.cm土层硝态氮积累量显著增加;随小麦生育进程推进,施氮处理上层土壤硝态氮下移趋势明显,至小麦成熟时,施氮1952～85.kg/hm2处理60100.cm土层硝态氮含量显著大于其它处理。小麦生长期间,0100.cm土层铵态氮积累量较为稳定,施氮处理间亦无显著差异。与不施氮肥相比,施氮提高小麦生长期间040.cm土层土壤微生物量氮含量;当施氮量小于240.kg/hm2时,随施氮量增加,土壤微生物量氮含量增加。小麦的氮肥利用率随施氮量增加而降低;施氮1051～95.kg/hm2,收获时小麦植株吸氮量、生物产量、子粒产量和子粒蛋白质含量提高;而施氮量大于240.kg/hm2时,小麦生育后期的氮素积累量降低,收获时植株吸氮量、生物产量和子粒蛋白质含量降低。说明本试验条件下,施氮1051～50.kg/hm2可满足当季小麦氮素吸收利用,获得较高的子粒产量和蛋白质含量。继续增加施氮量,土壤微生物量氮含量增加,但土壤中残留大量硝态氮,易淋溶损失。     

Use efficiency of nitrogen from biodigested plant material by ryegrass

Apparent nitrogen‐use efficiency of the applied mineral N (NUE_min) in effluent from biodigested plant material (BE; C : N_org ratio 14:1; mineral N–to–total N ratio 0.5:1) and a nitrate‐based inorganic fertilizer (IF), both applied at two rates was investigated in a six‐month pot experiment with Italian ryegrass (Lolium multiflorum Lam.). Dry‐matter (DM) production was 7% lower and total amount of N in aboveground biomass was 8% lower in BE than in IF at 40 d after sowing (DAS), equal at 81 DAS, and higher in BE than in IF at 136 and 172 DAS. NUE_min calculated on the basis of accumulated N in aboveground biomass of ryegrass in fertilized treatments compared to a control without N application was significantly lower in BE than in IF up to the third cut (136 DAS). Total NUE_min, total N recovery, and amount of foliage DM were similar for both fertilizers at the end of the experiment. Root biomass, total DM produced including roots and stubble, the fraction of root N to total plant N, and soil mineral N at 172 DAS were higher for BE than for IF. Mineral N applied with biogas‐reactor effluent was almost as effective as the nitrate‐based mineral fertilizer used for comparison. Within the six‐month experimental period net N mineralization, estimated at 12% of organic N in effluent, was not substantial. Hence, the organic compounds in the effluent were relatively recalcitrant.     

施氮量、土壤和植株氮浓度与小麦赤霉病的关系

【目的】赤霉病已成为影响小麦产量和品质的重要病害之一,为了解施用氮肥对小麦赤霉病的影响,本文通过研究不同施氮水平下小麦赤霉病的发病情况,探索施氮、土壤供氮、植株氮浓度与小麦赤霉病的关系。【方法】采用田间小区试验,以多穗型豫麦49-198(YM49-198)和大穗型周麦16(ZM16)为供试品种,设N 0、120、180、240、360 kg/hm25个施氮水平(N0、N120、N180、N240、N360),根据"小麦赤霉病测报技术规范"调查小麦赤霉病的发病情况。【结果】土壤硝态氮含量及0—90 cm土层土壤硝态氮累积量均随施氮量的增加而增加,小麦收获期N0、N120、N180处理0—30 cm土层硝态氮含量及0—90 cm累积量差异不显著,但显著低于N240和N360处理。两个品种小麦赤霉病病穗率和病情指数(DI)随施氮量的增加而增加,各处理间差异显著;豫麦49-198施氮处理的病穗率和DI比不施氮处理分别增加29.5%~132.0%和35.9%~225.2%,周麦16施氮处理的病穗率和DI比不施氮处理分别增加42.4%~161.8%和41.7%~206.9%;两个品种小麦N180处理赤霉病的病穗率和病情指数与N0、N120差异较小,显著低于N240和N360;周麦16较豫麦49-198发病严重,各处理的病穗率和病情指数比豫麦49-198分别高出7%~25%和28.0%~63.6%。小麦赤霉病病穗率和DI与硝态氮含量显著正相关,与0—90 cm硝态氮累积量呈线性正相关。孕穗期、开花期和灌浆期茎基部硝酸盐含量和拔节期~开花期植株的全氮含量各处理间差异较大,且与小麦赤霉病病穗率和DI显著线性正相关。【结论】土壤硝态氮含量及累积量随施氮量增加而增加,小麦收获后施氮量低于N 180 kg/hm2时土壤中硝态氮残留较低,赤霉病发病较轻。小麦赤霉病病穗率和病情指数随施氮量的增加而增加,说明施氮量过高会加重小麦赤霉病病害;小麦拔节期~开花期的氮浓度过高会加重赤霉病病害,因此在这一时期,适宜的施氮量、土壤硝态氮和植株氮浓度在赤霉病发生年份可以减轻病害,综合考虑土壤硝态氮残留、产量和赤霉病害等因素的适宜施氮量为N 180 kg/hm2。     

Arbuscular Mycorrhizal Mediated Nutrition in Plants

ABSTRACT

Arbuscular mycorrhizae (AM) are the symbiotic fungi that predominate in the roots and soils of agricultural crop plants. The most recognized beneficial effect of these fungi is to enhance host plant uptake of relatively immobile nutrients, in particular phosphorus (P), and several micronutrients. The AM fungi absorb inorganic P either from the soluble P pools in the soil, or from insoluble forms such as rock phosphates as well as from insoluble organic sources. Recent studies show that mycorrhizal fungi would have access to rock phosphate through localized alterations of pH and/or by the production of organic acid anions that may act as chelating agents. The AM colonization also improves plant N nutrition. Generally mycorrhizal symbiosis more influences on nitrogen (N) uptake and translocation if ammonium (NH₄ ⁺) rather than nitrate (NO₃ ^?) is the nitrogen source. However, under drought stress the role of mycorrhizae in NO₃ ^? transport to the root surface may be significant as the NO₃ ^? mobility is severely restricted due to its low concentration and diffusion rate under such circumstances. However, as yet little is known about the mechanism of N uptake by the AM fungi. Uptake of micronutrients is also influenced by mycorrhizal colonization.     

Dynamics of 15N-labeled ammonium sulfate in various inorganic and organic soil fractions of wetland rice soils

Summary The dynamics of basally applied ¹⁵N-labeled ammonium sulfate in inorganic and organic soil fractions of five wetland rice soils of the Philippines was studied in a greenhouse experiment. Soil and plant samples were collected and analyzed for ¹⁵N at various growth stages. Exchangeable NH₄ ⁺ depletion continued after 40 days after transplanting (DAT) and corresponded with increased nitrogen uptake by rice plants. Part of the applied fertilizer was fixed by 2:1 clay minerals, especially in Maligaya silty clay loam, which contained beidellite as the dominant clay mineral. After the initial fixation, nonexchangeable ¹⁵N was released from 20 DAT in Maligaya silty clay loam, but fixation delayed fertilizer N uptake from the soil. Part of the applied N was immobilized into the organic fraction. In Guadalupe clay and Maligaya silty clay loam, immobilization increased with time while the three other soils showed significant release of fertilizer N from the organic fraction during crop growth. Most of the immobilized fertilizer N was recovered in the nondistillable acid soluble (alpha-amino acid + hydrolyzable unknown-N) fraction at crop maturity. Between 61% and 66% of applied N was recovered from the plant in four soils while 52% of fertilizer N was recovered from the plant in Maligaya silty loam. Only 20% – 30% of the total N uptake at maturity was derived from fertilizer N. Nmin (mineral N) content of the soil before transplanting significantly correlated with N uptake. Twenty-two to 34% of applied N was unaccounted for possibly due to denitrification and ammonia volatilization.     

氮肥形态对马铃薯氮素积累与分配的影响

采用田间试验的方法,研究了不同氮肥形态对氮素在马铃薯不同器官中的吸收和运转分配及产量的影响。试验结果表明:铵态氮肥对马铃薯地上干物质积累量的增加作用最明显,施氮处理马铃薯块茎干物质积累量比对照增加50.37%～71.38%;马铃薯各器官中含氮量随生育期推进逐渐下降,其中,茎和叶下降幅度较大;马铃薯各器官中氮含量生育前期表现为叶片>地上茎>根,进入块茎形成期以后,则叶片>根>地上茎>块茎。施氮在马铃薯生育前期有利于茎对氮素的吸收和储存,后期又可以促进茎中的氮素向叶片和块茎转移。施氮各处理产量较对照增加19.28%～63.86%,NH4+-N处理对氮的吸收、积累与分配影响最大,且产量最高,达到39 410.2 kg.hm-2。     

Deep root growth and nitrogen uptake by rocket (Diplotaxis tenuifolia L.) as affected by nitrogen fertilizer,plant density and leaf harvesting on a coarse sandy soil

This study investigated management strategies to increase deep root growth and crop nitrogen (N) uptake by rocket grown as baby leaf in coarse sandy soil. Stage I (sowing to first harvest) measured the effects of two sowing densities and two N fertilizer rates on root growth and total N uptake. In Stage II (first to second harvest), effects of leaf harvesting and late season N fertilizer application on root growth, total N uptake and deep ¹⁵N uptake were measured. At the end of Stage I, root depth was 0.68–0.90 m, and the large fertilizer application increased N uptake. Plant density increased root depth, N uptake and nitrogen use efficiency (NUE) early in this stage and biomass production at harvest. Leaf harvesting in Stage II affected root density but not root depth that reached 1.4 m. The ability for N uptake was greater from 0.6 m due to more roots and larger N inflow than from 1.1 m depth. Late season fertilizer increased N concentration and uptake but did not affect NUE and deep N uptake. During the growing season, 330–349 kg N_inorg/ha was lost from 0 to 1.0 m depth most likely by leaching. Management practices that increased root growth and N uptake were found to increase NUE in rocket production early in the season. The production system used N inefficiently and smaller applications, plant density, leaf harvesting and other changes of management are required to reduce leaching.     

施氮水平对烤烟根冠平衡及氮素积累与分配的影响

在盆栽条件下,设不施氮（CK）,每株施N 5.45g（N1）和8.18g（N2）3个施氮水平,运用15N示踪技术,研究了不同施氮量条件下烤烟根冠平衡及氮素在不同器官间的积累与分配。结果表明,移栽至打顶期烤烟地上部干物质累积量随施氮量增加而增加,根系干物质积累量以N1处理最高;打顶至成熟期地上部干物质累积量N1处理最高,根系干物质积累量随施氮量增加而增加。打顶期根冠比随施氮量增加而降低,成熟期根冠比随施氮量增加而提高。打顶至成熟期烟株氮素积累量以N1处理最高;期间N1处理各器官均有一定氮素积累,而 N2处理和CK下部叶及中部叶有一定量的氮素输出。打顶期氮素在根系中的分配比例随施氮量增加而降低。随施氮量增加,烤烟积累的氮素中来自肥料氮的比例增加;积累的肥料氮中来自基肥氮的量增加。在本试验条件下,施氮（N）5.45 g/plant可促进根冠平衡,使烟株稳健生长。