Using High-Resolution Satellite Imaging to Evaluate Nitrogen Status of Winter Wheat

Nitrogen (N) applications often increase crop yields significantly, but N needs vary spatially across fields and landscapes. The color of the wheat plant is sensitive to N status and may provide a means to accurately predict N fertilizer rates matching spatial variability. Previous researches have reported that remote sensing may contribute to N management decisions by collecting spatially dense information. The objective of this study was to determine the feasibility of using high-resolution satellite imaging for evaluating N status of winter wheat in the North China Plain. High-resolution images from a QuickBird satellite were taken on April 1, 2002 at booting stage of wheat with multi-spectral wavelengths (blue, green, red, and near-infrared). Correlation analyses indicated that all the broadband indices derived from the satellite images correlated well with sap nitrate concentration, SPAD readings, total N concentration, and aboveground biomass. The individual band reflectance values R, G, B correlated well with sap nitrate concentration, SPAD readings, total N concentration, and aboveground biomass. These results demonstrated the potential of using new generation high-resolution satellite imaging for large area wheat N status diagnosis.     

华北地区采用无机氮测试和植株速测进行夏玉米氮肥推荐

A field experiment with a split-plot design was carried out at Dongbeiwang Farm in Beijing Municipality to establish reliable N fertilizer recommendation indices for summer maize (Zea mays L.) in northern China using the soil Nmin(mineral N) test as well as the plant nitrate and SPAD (portable chlorophyll meter readings) tests. The results showed that Nrnin sollwert (NS) 60 kg N ha^-1 at the third leaf stage and N rate of 40 to 120 kg N ha^-1 at the tenth leaf stage could meet the N requirement of summer maize with a target yield of 5.5-6 t ha^-1. Sap nitrate concentrations and SPAD chlorophyll meter readings in the latest expanded maize leaves at the tenth leaf stage were positively correlated with NS levels, indicating that plant nitrate and SPAD tests reflected the N nutritional status of maize well. Considering that winter wheat subsequently utilized N after the summer maize harvest, the 0-90 cm soil Nmin (74 kg N ha^-1) and apparent N loss (12 kg N ha^-1) in the NS60＋40 treatment were controlled at environmentally acceptable levels. Therefore NS60＋40, giving a total N supply of 100 kg N ha^-1, was considered the optimal N fertilizer input for summer maize under these experimental conditions.     

数字图像诊断技术在冬小麦氮素营养诊断中的应用

本文应用数码相机获取冬小麦冠层图像并对其进行相应色彩参数处理, 结合土壤、植株快速测试技术, 分析了色彩参数与传统氮素营养参数之间的数量关系, 研究了应用数字图像技术进行冬小麦氮素营养诊断的可行性, 建立了应用数字图像技术诊断冬小麦氮素营养状况的图像获取方法, 筛选出了适宜于冬小麦氮素营养诊断的最佳色彩参数以及最佳诊断时期, 建立了冬小麦氮素营养诊断指标体系和推荐施肥方程。研究结果表明, 数字图像技术可以作为冬小麦氮素营养诊断的方法。数字图像获取时, 可将数码相机与冬小麦冠层呈30°~60°角度进行拍摄。在冬小麦拔节期和孕穗期多数冠层图像色彩参数与施氮量、叶片SPAD 值、植株硝酸盐浓度、植株全氮含量、0~90 cm 土壤硝态氮含量之间存在显著或极显著相关关系; 在众多色彩参数中, 拔节期冠层图像绿光标准化值G/(R+G+B)与各项氮素指标的相关性最好, 可作为冬小麦氮素营养诊断的最佳色彩参数指标;拔节期可作为应用数字图像技术进行氮素诊断的关键时期。     

Effect of N supply on apparent recovery of fertilizer N as crop N and Nmin in soil during and after cultivation of winter cereals

Field trials were conducted over two years to investigate the effect of increasing N supply on apparent fertilizer N recovery by winter cereal crops (4 × wheat and 2 × barley) and on non‐recovered N. Apparent fertilizer N recovery was calculated by comparing N in fertilized and unfertilized crops. Non‐recovered N is defined as N which was neither found in crops nor soil mineral N (N_min = NH₄‐N + NO₃‐N). At N supply levels according to common farming practice (N_cfp = 190 to 220 kg N/ha), 60— 93% of the fertilizer N was recovered in crops at harvest, while at high N supply levels of 265 to 273 kg N/ha 58—76% of fertilizer N was recovered. There were small differences in soil N_min in 0—200 cm between N_cfp and unfertilized plots, but substantial increases in N_min occurred at the highest N supply. Amounts of non‐recovered N differed substantially between sites (maximum value of 84 kg N/ha). Non‐recovered N increased with increasing N rate on only 3 out of the 6 sites, indicating that N immobilization was not necessarily dependent on N rate. The fate of non‐recovered N was studied for a further year by growing catch crops on the sites after cereal harvest. N re‐mineralization deduced from changes in catch crop N and in N_min indicated that non‐recovered N had been immobilized in the soil. At three sites, crop N uptake was found between milk‐ripe stage and harvest (19 to 60 kg N/ha) suggesting substantial uptake of N mineralized from soil. However, grain yields were lower with N rates below N_cfp, indicating that late net soil N mineralization could not compensate for reductions in N fertilizer rate in these trials.     

无人机搭载数码相机航拍进行小麦、玉米氮素营养诊断研究

精准施肥是减少农业面源污染的重要技术之一,而土壤养分测试与作物营养诊断是其实施的技术保障,特别是在农业规模化经营方式下,急需发展快速、经济、无损的作物氮素营养诊断技术。本文在应用数字图像进行冬小麦、夏玉米氮素营养诊断研究的基础上,将数码相机搭载到无人机上,利用无人机航拍技术采集作物冠层数字图像,研究不同航拍高度下冠层图像相关色彩参数反演冬小麦和夏玉米氮素营养状态的差异,以确定适宜的航拍高度与敏感的色彩参数,建立利用无人机航拍数字图像诊断冬小麦和夏玉米氮素营养状态模型。研究结果表明:在冬小麦拔节期适宜的航拍高度是16 m,敏感的色彩参数是可见光大气阻抗植被指数(VARI),诊断模型为:冬小麦茎基部硝酸盐浓度=2.103 4e18.874VARI;夏玉米大喇叭口期适宜的航拍高度是50 m,敏感色彩参数是蓝光标准化值[B/(R+G+B)],诊断模型为:夏玉米第1完全展开叶叶脉硝酸盐浓度=1.526?1032?[B/(R+G+B)]50.445。依据建立的航拍方法与诊断模型,分别对冬小麦、夏玉米进行了氮素状态监测的验证,结果表明诊断结果与冬小麦、夏玉米实测数据的决定系数分别为0.80和0.85,且均在P0.01水平显著相关。最后将研究结果进行应用,生成了冬小麦、夏玉米氮肥追肥作业图。利用无人机搭载数码相机对冬小麦、夏玉米进行氮素营养诊断简单、可行,但仍有一些技术细节需要完善,以提高该技术的实用性。     

Validation and Recalibration of a Soil Test for Mineralizable Nitrogen

Abstract

A soil test for mineralizable soil N had been calibrated for winter wheat in the Willamette Valley of western Oregon. Seventy‐eight percent of the variation in spring N uptake by unfertilized wheat was explained by N mineralized from mid‐winter soil samples incubated anaerobically for 7 days at 40°C. Mineralizable N (Nmin) ranged from 10 to 30 mg N kg^?1 and was used to predict N fertilizer needs. Recommended rates of N were correlated (R²=0.87) with maximum economic rates of N fertilizer. Subsequent farmer adoption of no‐till sowing and a high frequency of soil tests>30 mg N kg^?1 prompted reevaluation of the soil test. Four N fertilizer rates [0, 56, G, and G+56 kg N ha^?1] were compared in 12 m×150 m farmer‐managed plots. Grower's N rates (G) ranged from 90 to 180 kg N ha^?1 and were based on N_min and NH₄‐N plus NO₃‐N soil tests. Averaged across ten no‐till and five conventionally tilled sites, grain yield and crop N uptake were maximized at the recommended rate of N. Results demonstrate that N fertilizer needs for winter wheat can be predicted over a wide range of mineralizable soil N (10 to 75 mg N kg^?1) and that the same soil test calibration can be used for conventionally sown and direct‐seeded winter wheat.     

Der Nitratgehalt in der Halmbasis als Maßstab für den Stickstoffdüngerbedarf bei Wintergetreide

The nitrate content of the basal internode of the stems as an indicator of the nitrogen fertilizer requirement of winter cereals In fertilizer experiments on loess soils in the southern part of Lower Saxony (FRG) the possibility was tested if the nitrate content of the basal internode of the stems can be used in the determination of the N-fertilizer requirement between stem elongation and ear emergence. The nitrate concentration was determined by a plant sap test. The following results were obtained:

• 1 Nitrate concentrations in the fresh matter between 200 and 2000 ppm can be determined by the reagent ?Diphenylaminesulphuric acid”?. This method is easily applicable and can be done by farmers.
• 1 Winter wheat and winter barley have different nitrate concentrations in the basal internode of the stems between stem elongation and ear emergence. As the N-supply in spring (N_min + N-fertilizer) was the same in all fields, the different nitrate concentrations in the stems are due to the preceding crop and to different organic manuring.
• 1 There is a close relationship between the nitrate content in stems (basal internode) and the nitrate content of the soil.
• 1 The required amount of nitrogen fertilizer between stem elongation and ear emergence was significantly correlated with the nitrate content in the stems (basal intenode). Therefore, a recommendation of N-top dressings to winter wheat and winter barley was made on this basis. The method is suitable for determining the necessary amount and timing of N-fertilization.

     

Vergleich von Nitrat- und Amino-N-Schnelltest zur Charakterisierung des Stickstoff-Versorgungsgrades von Winterweizen

Comparison of the rapid tests for nitrate and amino-N for evaluating the N-status of winter wheat Nitrogen fertilizer trials with winter wheat were conducted in 1985 and 1986 to compare the efficacy of the rapid test for nitrate (in the stem base) and a newly developed rapid test for amino-N (in fully expanded green leaves) for evaluating the N-status of plants. In addition, the influence of weather conditions on the results given by both tests when using ammonium nitrate (NH₄NO₃ + CaCO₃) (AN) and urea-ammonium nitrate solution (UAN), was evaluated to determine wether the rapid test for amino-N gives more accurate information on the N-status of winter wheat than the rapid test for nitrate. The results show that the rapid test for nitrate is suitable to characterize the N-status of winter wheat when nitrogen is predominantly taken up by roots as nitrate. This is normaly the case when plants are fertilized with the salt form of nitrogen, als well as with liquid fertilizer, such as UAN applied through tubes in the soil, as for fertilization of winter wheat at later growing stages. However, during dry weather (1986 field trial) UAN application on leaves at shooting and ear emergence can result in high nitrogen uptake by leaves, causing an underestimation of plant N-status by the rapid test for nitrate, and thus, subsequent excess N-fertilizer application may be recommended (order of magnitude: 20 kg N/ha). Under these conditions the rapid test for amino-N in leaves (pressed sap) is a more accurate test for estimation of plant N-status because it determines glutamine and amino acids, the most important storage forms of reduced nitrogen in plants. When UAN fertilizer on leaves is washed off by rain (1985 field trial), crop N-fertilizer requirements predicted by both tests are comparable. If storage of nitrate in the stem base occurs, due, for example to low radiation intensity during spring (lower nitrate reduction), with the rapid test for amino-N an underestimation of plant N-status can be obtained.     

秸秆还田替代化肥对黄土旱塬小麦产量及水肥利用的影响

为研究不同秸秆还田量替代部分化肥后对黄土高原冬小麦产量、水肥利用效率和硝态氮积累特征的影响。于2018—2021年在晋南黄土旱塬冬小麦种植区,设置秸秆不还田(S0)、秸秆半量还田(S1/2)、秸秆全量还田(S1)、秸秆2倍量还田(S2)4个还田量处理,研究不同秸秆还田量替代化肥对冬小麦产量形成、水肥利用效率及土壤硝态氮残留的影响。结果表明:在黄土旱塬麦区,秸秆还田替代8.3%~31.9% N和15.7%~63.2% P₂O₅的基础上,冬小麦产量总体随秸秆还田量增加而增加,且在降水丰沛年份,增加秸秆还田量可产生更大的产量效应。3年试验总体表明,S2处理冬小麦平均产量分别较S0、S1/2和S1处理分别高17.5%(P＜0.05),10.4%(P＜0.05),4.3%。连续3年秸秆还田均提高了冬小麦穗数,S2处理冬小麦平均单位面积穗数分别较S0、S1/2和[JP]S1处理高17.1%(P＜0.05),12.3%(P＜0.05),3.6%,不同处理间穗粒数和千粒重差异不显著。播前2 m土壤贮水量总体随着还田量的增加而增加,试验期间S2处理平均贮水量较S0提高8.3%(P＜0.05)。冬小麦生育期耗水量也表现为随着还田量的增加而增加,S2处理平均耗水量较S0处理增加了10.0%(P＜0.05)。不同处理间水分生产效率差异不显著,平均为14.9 kg/(hm²·mm)。在秸秆还田替代部分化肥基础上,旱塬冬小麦肥料利用效率随着秸秆还田量的增加而增加,其中,S2处理平均氮肥偏生产力(PFP_N)、氮肥农学效率(AE_N)、氮肥当季回收率(RE_N)和磷肥偏生产力(PFP_P)较S0处理分别提高66.4%,155.8%,113.5%,105.2%。连续3年秸秆不还田使0—2 m土壤硝态氮残留量较2018年播前提高100.6%,并随水向下淋溶在深层土壤中累积,而秸秆还田处理2 m土层硝态氮累积量均低于2018年播前,S2处理2 m土壤硝态氮残留量最低,为244.8 kg/hm²。综合考虑,晋南黄土旱塬麦区,在秸秆还田替代8.3%~31.9% N和15.7%~63.2% P₂O₅基地上,可增加播前土壤底墒,降低肥料残留,并提高肥料利用效率,进而提高冬小麦产量,其中,以2倍秸秆还田量(平均为7 477 kg/hm²)产生的产量和水肥效应最佳。研究结果可为推进旱作麦区面源污染防控和冬小麦高产高效绿色生产提供理论依据。     

施氮量、土壤和植株氮浓度与小麦赤霉病的关系

【目的】赤霉病已成为影响小麦产量和品质的重要病害之一,为了解施用氮肥对小麦赤霉病的影响,本文通过研究不同施氮水平下小麦赤霉病的发病情况,探索施氮、土壤供氮、植株氮浓度与小麦赤霉病的关系。【方法】采用田间小区试验,以多穗型豫麦49-198(YM49-198)和大穗型周麦16(ZM16)为供试品种,设N 0、120、180、240、360 kg/hm25个施氮水平(N0、N120、N180、N240、N360),根据"小麦赤霉病测报技术规范"调查小麦赤霉病的发病情况。【结果】土壤硝态氮含量及0—90 cm土层土壤硝态氮累积量均随施氮量的增加而增加,小麦收获期N0、N120、N180处理0—30 cm土层硝态氮含量及0—90 cm累积量差异不显著,但显著低于N240和N360处理。两个品种小麦赤霉病病穗率和病情指数(DI)随施氮量的增加而增加,各处理间差异显著;豫麦49-198施氮处理的病穗率和DI比不施氮处理分别增加29.5%~132.0%和35.9%~225.2%,周麦16施氮处理的病穗率和DI比不施氮处理分别增加42.4%~161.8%和41.7%~206.9%;两个品种小麦N180处理赤霉病的病穗率和病情指数与N0、N120差异较小,显著低于N240和N360;周麦16较豫麦49-198发病严重,各处理的病穗率和病情指数比豫麦49-198分别高出7%~25%和28.0%~63.6%。小麦赤霉病病穗率和DI与硝态氮含量显著正相关,与0—90 cm硝态氮累积量呈线性正相关。孕穗期、开花期和灌浆期茎基部硝酸盐含量和拔节期~开花期植株的全氮含量各处理间差异较大,且与小麦赤霉病病穗率和DI显著线性正相关。【结论】土壤硝态氮含量及累积量随施氮量增加而增加,小麦收获后施氮量低于N 180 kg/hm2时土壤中硝态氮残留较低,赤霉病发病较轻。小麦赤霉病病穗率和病情指数随施氮量的增加而增加,说明施氮量过高会加重小麦赤霉病病害;小麦拔节期~开花期的氮浓度过高会加重赤霉病病害,因此在这一时期,适宜的施氮量、土壤硝态氮和植株氮浓度在赤霉病发生年份可以减轻病害,综合考虑土壤硝态氮残留、产量和赤霉病害等因素的适宜施氮量为N 180 kg/hm2。     

Remote sensing of sugarbeet canopies for improved nitrogen fertilizer recommendations for a subsequent wheat crop

Abstract

Return to soil of high N, green sugarbeet (Beta vulgaris L.) tops, but not the return of low N, yellow to yellow‐green tops, reduces the magnitude of N‐fertilizer responses for the following crop. Twelve N fertilizer trials with spring wheat (Triticum aestivum L.) were established at sites with late‐season ‘green’ (8 sites) or ‘yellow’ (4 sites) sugarbeet canopies the previous year. Late‐season, aerial color photographs of sugarbeet fields and global positioning system (GPS) technology were used to locate the experimental sites. Based on the soil NO₃‐N test customarily used in the Northern Grain Plains, N fertilizer responses were expected at 11 of the 12 sites. However, no significant grain‐yield responses were obtained at the eight antecedent ‘green’ sugarbeet sites. Expected yield and grain‐N responses were obtained at the four antecedent ‘yellow’ sites. In contrast to the usual soil NO₃‐test, remote sensing of the previous sugarbeet crop resulted in successful prediction of N‐fertilizer responses at all 12 experimental sites. Application of N fertilizer at the ‘green’ canopy sites increased the likelihood that excess soil NO₃‐N would be present after the wheat harvest. A precision farming technique, involving remote sensing of late‐season sugarbeet canopies, use of GPS technology, and use of variable rate N‐fertilizer application is recommended for a wheat crop following sugarbeet.     

Importance of soil mineral N in early spring and subsequent net N mineralisation for winter wheat following winter oilseed rape and peas in a milder climate

Abstract

Nine biennial field experiments, 2000–2004, in south Sweden, 55–56°N, with winter wheat following winter oilseed rape, peas, and oats, were used to estimate the impact of a future milder climate on winter wheat production in central Sweden, 58–60°N. The trials included studies 1) on losses during winter of soil mineral nitrogen (N_min, 0–90 cm soil), accumulated after the preceding crops in late autumn, 2) on soil N mineralisation (N_net) during the growing season of the wheat (early spring to ripeness) and 3) on grain yield and optimum N fertilisation (Opt-N rate) of the wheat. Average N_min in late autumn following winter oilseed rape, peas, and oats was 68, 64, and 45 kg ha^?1, respectively, but decreased until early spring. Increased future losses of N_min during the winter in central Sweden due to no or very short periods with soil frost should enhance the demand for fertiliser N and reduce the better residual N effect of winter oilseed rape and peas, compared with oats. Their better N effect will then mainly depend on larger N_net (from March to maturity during the winter wheat year). Owing to more plant-available soil N (mainly as N_net) Opt-N rates were lower after oilseed rape and peas than after oats despite increased wheat yields (700 kg ha^?1) at optimum N fertilisation. In addition to these break crop effects, a milder climate should increase winter wheat yields in central Sweden by 2000–3000 kg ha^?1 and require about 30–45 kg ha^?1 more fertiliser N at optimum N fertilisation than the present yield levels. Increased losses and higher N fertilisation to the subsequent winter wheat in future indicates a need for an estimation of the residual N effect at the individual sites, rather than using mean values as at present, to increase N efficiency.     

Nitrogen accumulation efficiency: Relationship between excess fertilizer and soil‐plant biological activity in winter wheat

The point at which nitrogen (N) applied approaches 100% recovery in the soil once plant and microbial sinks have been saturated has not been determined in winter wheat (Triticum aestivum L.) production systems. In dryland winter wheat, subsoil accumulation has not been found to occur until N rates exceed that required for maximum yield. Many conventional N rate experiments have not properly evaluated subsoil N accumulation due to the lack of equally spaced N rates at the high end of the spectrum over which accumulation is expected to occur. Therefore, the objectives of this study were to (i) determine when soil profile accumulation efficiencies reach 100% in continuous winter wheat production and (ii) to evaluate the potential for nitrate‐nitrogen (NO₃ ^‐N) leaching in continuous winter wheat when extremely high rates of fertilizer N are used. Two field experiments (T505 and T222) were conducted for two years using ten N rates (preplant‐incorporated) ranging from 0 to 5376 kg N ha¹. No additional preplant fertilizer was applied in the second year. Following the first and second year wheat harvest, soil cores were taken to 2.4 m and bulk density, ammonium‐nitrogen (NH₄‐N) and NO₃‐N were determined. Crop N‐use efficiency (NUE) (N uptake treated ‐ N uptake check/rate applied) and soil profile inorganic N accumulation efficiencies (NAE) [net inorganic N accumulation in the soil profile/(fertilizer applied ‐ net N removed in the crop)] changed with fertilizer rate and were inversely related. Priming (increased net mineralization of organic N pools when low rates of fertilizer N are applied) may have occurred since increased NUE was observed at low N rates. The highest N‐accumulation efficiencies were at N rates of 168 and 448 kg ha^‐1 in experiments T505 and T222, respectively. At both T222 and T505, no subsoil accumulation of NH₄‐N or NO₃‐N beyond 100 cm was observed for any of the N treatments when compared to the 0‐N check, even when N rates exceeded 448 kg ha^‐1.     

应用IKONOS卫星影像监测冬小麦氮营养状况

应用从高精度卫星影像获取的光谱植被指数和单波段光谱反射值与冬小麦拔节期氮素营养状况进行了相关分析,结果发现:高精度卫星光谱归一化植被指数、绿植被指数、比值植被指数、优化土壤调节植被指数和单波段光谱反射值红外波段与拔节期小麦叶片叶绿素仪读数、茎基部硝酸盐含量、地上部生物量和地上部吸氮量等都有显著的线性正相关关系,相关系数范围在0.651~0.860之间。而红光波段、绿光波段则与拔节期小麦氮营养诊断指标呈显著线性负相关关系,相关系数范围在-0.803~-0.574之间。且上述光谱植被指数和单波段反射值均与小麦拔节期优化施氮量有很好的相关关系。因此,可以应用高精度卫星影像结合地面土壤植株测试进行冬小麦拔节期的冠层氮营养诊断和氮肥推荐。     

Soil fertility in a long‐term fertilizer trial with different tillage systems

The presented results originate from a field experiment established in 1972 on an Eutric cambisol with two main factors: soil tillage (conventional‐, reduced‐, and no‐tillage) and NPK fertilization. The test plants were maize and winter wheat in two years rotation.

The long‐term soil fertility without and with optimum fertilization, the influence of fertilization, tillage and crop sequence on grain yields, the organic carbon content (C_org) and the nitrate infiltration are discussed.

In the course of years without any NPK fertilization grain yields of maize and winter wheat decreased significantly and reached a minimum level which was modified however by the actual climatic conditions. The analogous yield level of optimum NPK fertilization at maize showed a growing tendence while at wheat it remained mostly constant.

The method of soil tillage influenced grain yield of winter wheat to a lesser extent than the yield of maize. Grain yields of maize and winter wheat were consistently lower with no‐till as compared to reduced or conventional tillage, however the differences with w. wheat were much smaller. The effect of tillage was especially high at N₀ P₀ K₀. Crop rotation had a positive effect on the yields of maize. For winter wheat at N₀ P₀ K₀ oneself was the better forecrop, while at optimum N and PK maize performed a little better. C_orgcontent of soil slightly increased in the course of 25 years not only on the fertilized plots but on the nil plots too. Increasing N‐doses showed only a little effect on the C_org. There was a little positive effect of no‐till on C_org content of soil as well as compared fall ploughing. Soil tillage did not much influence the total amount of nitrate in the soil profile. The distribution of nitrate‐N in the soil profile was more affected by the actual climatical circumstances than by the system of tillage. However big nitrate accumulations were found in the subsoil according to different soil tillage systems at some other times, as well as lack of it, which suppose the possibility of a relative quick nitrate infiltration.     

Nmin−Gehalt im Boden,mineralische N-Düngung und N-Entzug von Winterweizen im Internationalen Organischen Stickstoffdauerdüngungsversuch (IOSDV) Berlin-Dahlem

N_mim content in the soil, N-fertilization and N uptake of winter wheat in the international organic nitrogen long-term fertilization experiment (IOSDV) Berlin-Dahlem During the 9th and 10th year of the long-term IOSDV field experiment micro plots were put in three treatments. Labelled¹⁵ N (160 resp. 110 kg/ha N as ammonium sulfate) was fertilized to winter wheat subdivided into three portions. N_min in soil was determined five times during the season, plant biomass was harvested at different growth stages and N uptake was calculated. Using the¹⁵ N-technique permitted a discrimination between fertilizer-N and soil-N. Preferential uptake of fertilizer-N by the wheat crop but also immobilisation in soil were observed until June. Subsequently the immobilized N was remineralized and assimilated by wheat. But the native N_min of soil was minimaly utilized during the initial growth of wheat. Therefore the absolute amount of soluble N temporarily increased caused by a mineralisation of the organic matter.     

数字图像技术在夏玉米氮素营养诊断中的应用

基于6个不同水平的氮肥田间试验,采用数码相机获取夏玉米6叶期和10叶期的冠层图像,分析了不同供氮水平下夏玉米冠层图像色彩参数指标与施氮量、叶片SPAD值、植株硝酸盐浓度、植株全氮含量、0～90cm土壤硝态氮含量之间的关系。结果表明:在6叶期,玉米冠层数字图像色彩参数指标B/(R+G+B)、G/B、R/B、B/L均与施氮量、叶片SPAD值、植株硝酸盐浓度、植株全氮含量、0～90cm土壤硝态氮含量存在极显著的线性相关关系,其中B/(R+G+B)与各营养参数的相关关系最好,其次是B/L。因此,运用数字图像技术进行玉米的氮素营养诊断是可行的。夏玉米6叶期冠层图像色彩参数指标与上述营养参数间的相关性明显高于10叶期,可作为应用数字图像技术进行氮素营养诊断的关键时期,而蓝光标准化值[B/(R+G+B)]是进行夏玉米氮素营养诊断的最佳冠层图像色彩参数指标。     

华北平原冬小麦/夏玉米轮作体系对氮素环境承受力分析

通过田间试验研究了华北地区冬小麦/夏玉米轮作体系对氮素的环境承受力。结果表明,冬小麦和夏玉米达到最高产量时的施氮量分别是112和180.kg/hm2。氮肥利用率和农学利用率随施氮量的增加而降低,生理利用率表现出抛物线的趋势。在农户习惯施氮条件下,冬小麦和夏玉米的氮肥利用率分别是10%和6%,每千克氮肥分别增产2和3千克。灌水和集中降雨是引起土壤硝态氮明显下移的主要因素。氮素平衡计算的结果表明,低施氮量时,氮素盈余以残留Nmin为主,高量施氮则以表观损失为主。将收获后090.cm土壤中的硝态氮的量控制到150kg/hm2,可以在兼顾环境的前提下获得较高的产量;此时冬小麦季的施氮量是122.kg/hm2,产量(干物重)达到最高产量4331.kg/hm2;夏玉米季的施氮量是145.kg/hm2,产量(干物重)是7965.kg/hm2,达到最高产量的97%。     

On-farm trials of site-specific N management for maximum winter oilseed rape (Brassica napus L.) yield

Site-specific nitrogen (N) fertilizer strategy based on soil mineral N (N_min) test is crucial for maintaining high crop yield and high N-use efficiency. A two-year field experiment was conducted to develop a site-specific N fertilizer management for winter oilseed rape in the 2011–12 and 2012–13 seasons in Wuhan, central China. In contrast to fixed N fertilizer recommendation (FN), the use of the N_min test could optimize the N fertilizer inputs in time to fulfill crop N uptake during different growth stages and achieve high seed yield. Despite annual variations in seed yields and N fertilizer recommendations, the N recovery efficiency of the site-specific N fertilizer (SN) treatment was higher than that of the FN treatment. Consequently, the soil-based N strategy matches crop N uptake and soil N supply and achieves high yield depending on the site-specific soil-crop conditions.     

Effect of increasing rates of 15N‐labelled fertilizer on recovery of fertilizer N in plant and soil N pools in a pot experiment with winter wheat

The effect of increasing rates of ¹⁵N‐labelled Ca(NO₃)₂ (N₀ = no N application, N₃₀₀ = 300 mg N/pot; N₆₀₀ = 600 mg N/pot; N₉₀₀ = 900 mg N/pot) on recovery of fertilizer N in winter wheat plants and soil (total soil N, soil microbial biomass N [N_mic], extractable organic N [N_org]) and on N mineralization (NM_soil) was investigated at milk‐ripe growth stage in a pot experiment. The N rates were equally split at tillering, stem elongation and ear emergence. Fertilizer N recovered in crops increased with increasing N rates (N₃₀₀: 223.5 mg N/pot [74.5% of applied fertilizer N], N₆₀₀: 445.6 mg N/pot [74.3%], N₉₀₀: 722.1 mg N/pot [80.2%]). NM_soil slightly increased from N₀ (43.8 mg N/pot) to N₉₀₀ (75.6 mg N/pot) indicating that N application enhanced availability of soil‐derived N for the plants. However, in fertilized treatments NM_soil is balanced by immobilization and losses (non‐recovered fertilizer N). Therefore the effective soil N mineralization is indicated by apparent net N mineralization (ANNM = NM_soil — fertilizer N immobilization — lost fertilizer N). Fertilizer N immobilization in soil increased from N₃₀₀ (38.7 mg N/pot) to N₆₀₀ (60.7 mg N/pot) and N₉₀₀ (65.5 mg N/pot). Lost fertilizer N increased from N₃₀₀ (14.8 mg N/pot) to N₆₀₀ (56.7 mg N/pot) and N₉₀₀ (62.1 mg N/pot). As a consequence negative ANNM values were calculated at N₆₀₀ and N₉₀₀. Due to the small differences between N₆₀₀ and N₉₀₀ fertilizer N immobilization and lost fertilizer N did not increase linearly with increasing N rates, i.e. both processes were limited by factors other than N rate. Only 5.6—7.4% of the immobilized fertilizer N was recovered in N_org and 5.4—9.3% in N_mic soil pools. It is assumed that most of the immobilized fertilizer N was in non‐extractable organic N forms. N_mic and N_org were weak indicators for the extent of fertilizer N immobilization.