红壤侵蚀裸地不同植被恢复后林地土壤微生物特性的研究

本研究以中国科学院红壤生态实验站侵蚀地植被恢复试验区为依托,调查侵蚀裸地不同植被恢复类型林地表层土壤微生物特性.结果表明:侵蚀裸地种植植被后,表层土壤酸化程度减小,土壤养分含量和孔隙度显著提高,微生物生物量增加、活性增强;不同植被恢复类型相比,各指标的大小顺序均为樟树>胡枝子>马尾松;侵蚀裸地植被恢复前后表层土壤均以细菌为主,其中马尾松林地表层土壤真菌比例最高:植被恢复林地土壤有机C处于累积阶段,土壤微生物对C的利用率显著提高,但不同植被恢复类型之间无显著差异.     

南方侵蚀治理区土壤碳分布及主控因素研究

研究侵蚀治理区土壤碳区域分布影响因素对揭示退化土壤有机碳恢复有重要的意义。本文以江西省兴国县侵蚀治理恢复区林地土壤有机碳为研究对象,评价了土壤类型和成土母质对土壤有机碳的影响及其主控作用。结果表明：兴国县侵蚀治理恢复区林地土壤有机碳受土壤类型和成土母质影响显著,其中黄红壤各土层土壤有机碳含量均显著高于红壤;千枚岩发育的土壤有机碳显著高于红砂岩和花岗岩。对比土壤类型和成土母质对土壤有机碳的影响作用大小发现,在表层0 ~ 20 cm土壤类型对土壤有机碳变异解释能力大于30%,成土母质的解释能力约为17%,土壤类型是影响表层土壤有机碳的主控因素;表下层20 ~ 30 cm 土壤有机碳变异的解释能力则表现为成土母质(28.8%)与土壤类型(27.5%)基本相当,同为主控因素。因此,兴国县侵蚀治理恢复区土壤类型和成土母质对土壤有机碳的影响作用不容忽视,合理地分区展开水土保持工作有利于退化土壤的碳恢复。     

川西亚高山云杉人工林恢复过程中土壤有机碳矿化研究

研究了川西亚高山云杉(Picea asperata Mast.)人工林不同阶段(22、47、65年)及原始林表层(0～30cm)土壤有机碳的矿化,并用两室模型对实验数据进行了回归。结果表明,不同恢复阶段云杉人工林各层土壤(0～10、10～20、20～30cm)有机碳64天累积释放的CO2-C显著大于原始林。两室模型模拟土壤有机碳矿化过程得出土壤活性有机碳(AC)含量及其占总有机碳含量的比例均大于原始林,表明原始林转化为云杉人工林后,土壤有机碳稳定性降低。此外,本文分析了凋落物、微生物、土壤理化性质等因素对云杉人工林土壤有机碳矿化的可能影响。     

亚热带红壤区不同植被恢复类型土壤有机碳δ13C特征

为探究亚热带红壤区不同植被恢复类型对土壤有机碳周转的影响,本研究以自然恢复草地、马尾松林和木荷林为研究对象,测定植被和土壤有机碳的δ¹³C,探讨不同林分类型对土壤有机碳来源的影响。结果表明,自然恢复草地植被以C₄植物为主,退化草地转变为马尾松林和木荷林后,植被类型以C₃植物为主。2种林分从凋落物δ¹³C到表层土壤有机碳δ¹³C增加幅度超出了正常的增加范围,显示两个林地有机碳来源为原有草地和当前恢复林分的混合物,表层土壤来源于恢复林分的有机碳低于来源于原有草地的有机碳;2个林分土壤有机碳δ¹³C均随着深度增加而不断增大。木荷林土壤有机碳的分解速率低于马尾松林,木荷林中土壤有机碳存留时间高于马尾松林。综上所述,尽管马尾松林土壤有机碳来源于新碳的比例高于木荷林,但木荷林中土壤有机碳分解更慢,因此对马尾松林和木荷林土壤的固碳过程需要更加深入的研究。本研究为退化红壤区植被恢复树种的选择提供了理论依据,丰富了南方退化红壤区植被恢复林土壤有机碳研究。     

土地利用和轮作方式对旱地红壤生化性质的影响

研究不同土地利用和轮作方式对旱地红壤肥力的影响对提高红壤质量具有十分重要的指导意义。本研究以湖南省桃源县的林地、大豆-油菜轮作、玉米-休闲轮作土壤为研究对象,明确了林地、农地土壤及农地不同轮作方式对土壤化学和生物性质的影响。结果表明,林地土壤的pH、有机碳、速效养分、微生物生物量碳及酶活性(纤维素酶、酸性磷酸酶、转化酶、蛋白酶)均显著高于农地土壤;大豆-油菜轮作土壤的pH、养分含量、微生物生物量碳含量及其微生物熵在大多数情况下高于玉米-休闲轮作,但轮作处理对各酶活性的影响并不完全一致。这种不一致性可能与不同酶对由不同利用和轮作方式导致的土壤性质差异的敏感性不同所致。土壤有机碳和pH与各生物指标均呈显著正相关关系,表明提高该地区的土壤有机碳含量对于维持土壤的生化性质具有重要的作用。     

子午岭地区林地破坏加速侵蚀对土壤养分流失和微生物的影响研究

以浅沟集水区为研究对象,分析了子午岭地区林地被开垦破坏15年后裸露地在不同侵蚀强度和侵蚀方式下的土壤养分流失和土壤微生物数量的变化。结果表明,林地开垦破坏后,土壤侵蚀加剧发展,侵蚀强度达159.7t/(hm2a),是林地土壤侵蚀量的上千倍。开垦破坏15年后,裸露地浅沟集水区不同地形部位表层土壤全氮、有机碳、速效磷和土壤微生物总数显著减少,同林地相比,依次分别减少37.9%～82.6%、42.7%～86.4%、24.2%～80.3%和31.8%～92.0%。在裸露地浅沟集水区梁坡随坡长的增加,表层土壤有机碳、全氮和速效磷含量及微生物总数呈显著的下降趋势,且沟槽的土壤各养分含量及微生物总数明显低于沟间。裸露地浅沟集水区土壤养分流失强度及微生物数量减少幅度在浅沟集水区的空间分布与土壤侵蚀方式和侵蚀强度相对应。林地开垦破坏15年后,土壤养分以有机碳流失最严重,其次分别为速效磷、全氮;微生物中的真菌减少幅度最大,细菌次之,放线菌减少幅度最小。     

淮南大通矿区复垦土壤微生物量碳氮的分布特征

通过野外调查和室内分析,研究了淮南大通煤矿复垦区表层(0—10cm)土壤理化性质及微生物量碳(SMBC)、微生物量氮(SMBN)的分布特征。结果表明,耕地(油菜地、小麦地、蔬菜大棚)土壤有机碳、氮、磷等含量均高于林地,而土壤pH值则相反。土壤SMBC含量依次为:蔬菜大棚油菜地小麦地林地对照样,蔬菜大棚显著高于其他农业用地方式。土壤SMBN则为:蔬菜大棚小麦地油菜地林地,林地土壤微生物量氮显著低于其他农业用地方式。林地土壤微生物商最高,表明在有机质积累的同时林地更有利于土壤微生物活动。土壤微生物量碳氮之间显著相关,进一步证实土壤微生物量碳、氮可以作为表征土壤肥力的敏感因子。     

桉树凋落物对土壤微生物群落的影响:基于控制实验研究

全面认识桉树种植对土壤微生物群落结构和功能的影响及机制,对于阐明单一物种对生态系统服务功能的影响具有重要意义。通过室内小盆模拟控制试验,采用随机区组设计,以土壤碳、氮含量有显著差异的3种天然次生林土壤为对象,以不添加凋落物的处理和添加天然次生林混合凋落物的处理为对照,研究桉树凋落物对土壤微生物群落结构及功能的影响。结果表明:(1)与天然次生林的混合凋落物相比,桉树凋落物具有较高的碳含量和较低的氮含量,其碳氮比也较高;(2)添加桉树凋落物的土壤中细菌、真菌、放线菌以及磷脂脂肪酸的总丰度显著高于不添加凋落物的土壤,但是显著低于添加天然次生林混合凋落物的土壤,并且不同凋落物处理下土壤微生物群落的磷脂脂肪酸组成存在显著差异;(3)不同凋落物处理下土壤微生物群落的碳代谢方式差异显著,添加桉树凋落物的土壤微生物群落的碳代谢功能优于未添加凋落物的处理,但是显著低于天然次生林混合凋落物处理的土壤,包括:碳代谢的活性和多样性。综上所述,与天然次生林本身的凋落物相比,桉树凋落物影响下的土壤微生物群落的生物量、多样性和代谢活性均较低,表明桉树凋落物为土壤微生物群落提供生境和食物的能力较弱。     

不同状态高寒草原主要土壤活性有机碳组分的变化

对藏北高原正常、轻度和严重退化高寒草原表层(0 ～ 10 cm)、亚表层(10～20 cm)活性有机碳(Active soil organic carbon,ASOC)主要组分变化,以及土壤微生物对ASOC的影响进行了研究,结果表明:(1)易氧化有机碳(Readily oxidizable organic carbon,ROC)、微生物生物量碳(Microbial biomass carbon,MBC)、轻组有机碳(Light fraction organic carbon,LFOC)和水溶性有机碳(Water-soluble organic carbon,WSOC)对土壤环境变化的敏感度显著不同,平均分配比率分别为11.10％、0.57％、0.04％和0.03％,高原寒旱环境对WSOC、LFOC的形成与积累极为不利.(2)不同状态高寒草原亚表层ASOC各组分含量均显著高于表层;与正常草原ASOC各组分含量相比,退化草原表层、亚表层分呈小幅增加和大幅下降,但轻度退化草原变化幅度大于严重退化草原;因此,0～20 cm土层ASOC各组分含量均呈正常草原＞严重退化草原＞轻度退化草原.(3)不同状态草原中,纤维素分解酶活性对ASOC组分的形成均具极显著(R2:0.731 ～0.960)的促进作用,土壤放线菌、真菌对纤维素分解酶活性(Cellulolytic enzyme activity,CEA)则具有较大影响.(4)草原严重退化阶段,土壤微生物可能已完成向抗逆能力、纤维素分解酶分泌能力更强生理种群的演替,其相对较高的SOC、ASOC含量表征着土壤有机残体的较大消耗.     

不同处理采伐剩余物对营造杉木幼林土壤胞外酶活性的影响

土壤酶对森林生态系统生物化学过程有重要作用,能快速反映土壤环境(如养分含量和有效性)的变化。采伐剩余物是林地土壤养分的重要来源,其处理方式会对森林土壤的养分含量和有效性产生影响。为探讨采伐剩余物不同处理方式对杉木幼林土壤酶活性的影响,在福建省三明市格氏栲自然保护区40年生的杉木成熟林采伐迹地上对采伐剩余物进行不同处理(保留RR、火烧RB、去除R)并种植杉木幼林,通过采集0-10,10-20 cm土层土壤,对6种胞外酶活性进行研究。结果表明:(1)采伐剩余物处理4年后,相比保留处理,火烧和清除处理的土壤可溶性有机碳、可溶性有机氮、无机氮、硝态氮含量均显著下降。其中火烧处理下硝态氮下降幅度显著高于清除处理,2个土层下降幅度平均分别为88%和51%;(2)相比保留处理,火烧和清除处理土壤微生物生物量碳和微生物生物量磷含量以及6种土壤酶活性,即酸性磷酸酶、β-葡萄糖苷酶、纤维素水解酶、β-N-乙酰氨基葡萄糖苷酶、过氧化酶、酚氧化酶均明显更低,而且火烧处理两土层微生物生物量碳和微生物生物量磷含量显著低于清除处理;(3)冗余分析表明,0-10 cm土层土壤酶活性与微生物生物量磷、微生物生物量碳含量显著相关,而可溶性有机氮、硝态氮、微生物生物量碳是影响10-20 cm土层土壤酶活性变化的关键因子。保留采伐剩余物有利于提高土壤养分和酶活性,是土壤肥力维持和森林生产力提高的有效经营管理措施。     

Soil fauna modifies the recalcitrance-persistence relationship of soil carbon pools

Traditional models of soil organic matter decomposition predict that soil carbon pools with high chemical stability and large physical structure are more resistant against degradation than chemically labile and fine-grained material. We investigated whether soil fauna, by its direct and indirect effects on carbon turnover, would reinforce or counteract this general trend.The effects of four major faunal groups on carbon pools of differing recalcitrance were studied in an extensive microcosm experiment. Ninty-six microcosms were inoculated with nematodes, enchytraeids, collembola, and lumbricids in three densities, including combinations of groups. Bare agricultural soil and soil covered with maize litter were used as substrates. The microcosms were kept under constant conditions at 12 °C and 50% water holding capacity for 60 days. At the end of the experiment, soil particles were separated into size classes (<63 μm, 63-250 μm, >250 μm) and carbon pools were separated into solubility fractions (K₂SO₄-soluble, pyrophosphate-soluble, insoluble), by means of ultrasonic dispersion and subsequent stepwise solubilisation.Both in bare soil and in soil with litter, the carbon pools with the highest chemical stability (insoluble) and the larger particle sizes (>63 μm) were degraded more intensively than all other pools in the presence of lumbricids. The pools of intermediate chemical stability (pyrophosphate-soluble) underwent simultaneous degradation and neoformation brought about by different animal groups. The chemically most labile pool (K₂SO₄-soluble) remained largely unaffected by the fauna. Fixation of carbon in microbial biomass was increased by nematodes in bare soil and by enchytraeids in soil with litter. The results illustrate in detail how, under the influence of soil fauna, soil carbon pools are decomposed in a cascade-like process where carbon is transferred from the stable to the more labile pools, while simultaneously a proportion is fixed in microbial biomass and another part is lost as CO₂. Thereby, the relationship between a substrate's persistence and its chemical stability and physical size is substantially modified. We summarize the mechanisms that most likely are responsible for the different effects of the investigated faunal groups.     

Short-term and long-term effects of human trampling on above-ground vegetation, soil density, soil organic matter and soil microbial processes in suburban beech forests

Understanding the effects of disturbance by human trampling on ecosystem processes is essential for the management of recreational areas. Discussions on recreational impacts are based either on data from trampling experiments or on field survey data from sites subjected to long-term recreational use, but rarely on a combination of both. We examined whether results from a short-term trampling experiment reflect the impact of long-term trampling around frequently used fire places. We compared short- and long-term effects of human trampling on above-ground forest vegetation and soil physical, chemical and microbial characteristics. We found both similarities and differences in short- and long-term trampling effects. Both short- and long-term trampling reduced plant cover, plant height and species density, though long-term effects were more pronounced than short-term effects. In both approaches, leaf litter biomass decreased, whereas soil density increased with trampling intensity. Other soil characteristics including soil moisture, total soil organic matter content and total organic nitrogen content were not or only marginally affected by short- and long-term trampling. Furthermore, soil microbial biomass and the activity of dehydrogenase did not change in both approaches. In contrast, the activity of β-glucosidase was only reduced by short-term trampling, whereas activity of phosphomonoesterase was reduced only by long-term trampling. Soil compaction was one factor reducing microbial activities at low and medium trampling intensities in our experiment and in the highly compacted area around the fire rings. We conclude that it could be problematic to use the results of short-term trampling experiments to predict general long-term trampling effects. Our results imply also that the restoration of degraded sites might be hampered by the low nutrient turnover resulting from the reduced litter layer and changes in enzyme activities, mitigating a successful re-establishment and growth of plants.     

西双版纳热带雨林次生林的生物养分循环

本文获得了西双版纳热带雨林，砍伐后２块处于恢复演替阶段不同年代的次生林的生物量、生长量、年凋浇物量及它们的主要营养元素含量、对土壤养分状况的影响、以及土壤微生物状况和土壤生化活性等一系列资料。阐明了次生林生物物质和养分吸收、积累和归还的特点及对土壤养发状况的影响，研究了土壤微生物对凋落物的分解作用，从而对热带雨林次生林的生物养分循环作了一初步的探讨，为热带森林生态系统研究奠定了基础。研究表明：１．     

猪粪与化肥配施对植烟土壤酶活性和微生物 生物量动态变化的影响

采用盆栽试验研究不同用量的猪粪与化肥配施对植烟土壤酶活性和微生物生物量碳氮动态变化的影响。结果表明:植烟土壤中脲酶、过氧化氢酶、蔗糖酶活性和微生物生物量碳含量随栽培天数的增加呈动态变化,表现出先增加后减少的趋势,且在栽培10天或25天达到最高峰,各处理中以配施30%猪粪+70%化肥处理较好;微生物生物量氮含量随栽培天数增加表现出先下降后上升再下降的趋势,且在20天时达到最高峰,以化肥处理最好,配施处理以70%猪粪+30%化肥处理较好。综合所有指标,在该试验条件下,配施30%猪粪+70%化肥处理对植烟土壤酶活性和微生物生物量碳含量作用较好。     

How different reforestation approaches affect red soil properties in southern China

Significant differences were found in soil physicochemical and biological effects between various forest restoration approaches of a hilly red‐soil region, southern China. Soil quality was the highest in natural secondary forest (0·95), while in sites revegetated with tea‐oil camellia (Camellia oleifera), Chinese fir (Cunninghamia lanceolata) and slash pine (Pinus elliottii) and control sites, integrated soil quality indices were 0·68, 0·55, 0·36 and 0·04 respectively. The lower soil quality of plantations and controls resulted from increased disruption of soil physical structure, lower quality litter fall, lower litter fall production, reduction in microbial biomass, decline of microbial function and loss of soil nutrients due to periodically artificial tending and accelerated soil erosion. Improvement in soil physicochemical properties and enhanced soil microbial function at a natural secondary forest site demonstrated the inherent restoration of these soils. Hence, natural restoration, as well as reducing human disturbance, is a better approach to improving soil properties than returning farmland to planted woodlands. Copyright © 2005 John Wiley & Sons, Ltd.     

Soil microbial activity and litter turnover in native grazed and ungrazed rangelands in a semiarid ecosystem

Long-term overgrazing is known to influence soil microbiological properties and C sequestration in soil organic matter. However, much remains to be known concerning overgrazing impacts on soil microbial activity and litter turnover in heavily grazed rangelands of Central Iran. Aboveground litter decomposition of three dominant species (Agropyron intermedium, Hordeum bulbosum, and Juncus sp.) were studied using a litter bag experiment under field conditions in three range sites of Central Iran, a site with continuous grazing, a site ungrazed for 17 years with dominant woody species (80% cover), and a site ungrazed for 17 years with dominant pasture species (70% cover). Soil samples were taken from 0 to 30 cm depth and analyzed for their chemical and microbiological properties. Results demonstrate that soil organic C and total N contents and C/N ratios were similar for both ungrazed and grazed sites, while available P and K concentrations significantly decreased under grazed conditions. It was also evident that range grazing decreases soil respiration and microbial biomass C, suggesting a lower recent annual input of decomposable organic C. Nevertheless, grazing conditions had no significant effect on litter decomposition indicating soil microclimate is not affected by grazing animals in this ecosystem. It is concluded that overgrazing may presumably depress microbial activity through either reduced input of fresh plant residue into the surface soil or lack of living roots and exudates for stimulating microbial activity. This study also suggests that 17 years of livestock exclusion might be insufficient time for expected C accumulation in soil.     

The influence of soil compaction and the removal of organic matter on two native earthworms and soil properties in an oak-hickory forest

 Earthworms may alter the physical, chemical, and biological properties of a forest soil ecosystem. Any physical manipulation of the soil ecosystem may, in turn, affect the activities and ecology of earthworms. The effects of removing organic matter (logs and forest litter) and severely compacting the soil on native earthworm species were measured in a central USA hardwood region (oak-hickory) forest in the Missouri Ozarks (USA). Soils in this region are characterized by a cherty residuum that is primarily of the Clarksville series (Loamy-skeletal, mixed, mesic Typic Paledults). Earthworms were collected from 0–15 cm depth each spring and fall for 2 years by handsorting, and densities were determined on a per meter square basis. Two native earthworm species, Diplocardia ornata and Diplocardia smithii, were dominant on this site. Organic matter removal decreased the average individual biomass of both species. However, both species responded differently to soil compaction. Soil compaction affected D. ornata adversely and D. smithii favorably. This suggested that the degree of soil compaction was not as restrictive with respect to D. smithii (2 mm diameter) as to D. ornata (5 mm diameter). Moreover, the apparently improved soil environmental conditions resulting from the remaining organic matter in compacted soil enhanced the population and growth of D. smithii. Sampling position on the landscape affected D. ornata but not D. smithii. Soil microbial biomass C and soil microbial biomass N were decreased under soil compaction when the organic matter was removed. Other factors influencing the ecology and activity of these two species will require further study. Received: 6 January 1999     

退化红壤不同植被恢复下土壤微生物量季节动态及其指示意义

不同植被恢复下退化红壤恢复 1 0a后土壤微生物生物量 (C、N)的季节动态分析表明 :相比侵蚀裸地 ,植被恢复显著提高了土壤微生物量。不同植被对微生物量的影响具有极显著差异 (p <0 0 1 ) ,0～5cm土层的季节平均值顺序为 :保护荒草地 >干扰荒草地 >小叶栎 >混交林≥木荷 >马尾松 ;不同植被下微生物量N的差异更为明显。土壤微生物量呈高度季节变异 ,植被与季节存在极显著的交互作用 (p<0 0 1 ) ,但季节的影响低于植被 ;植被和季节的影响主要反映在 0～ 5cm土层。微生物量与土壤有机质及其它生物活性指标高度相关 (p<0 0 1 ) ,且能很好地区分不同植被间的差异 ,在红壤质量指示方面潜力很大。利用微生物量监测红壤质量恢复 ,建议春季为采样期 ,至少单独分析 0～ 1 0cm土层     

薄层黑土微生物生物量碳氮对土壤侵蚀—沉积的响应

研究土壤侵蚀—沉积对土壤微生物生物量的影响可以为科学评估土壤侵蚀的环境效应提供依据。以典型薄层黑土区——黑龙江省宾州河流域为研究区,利用土壤137Cs含量估算侵蚀速率,通过分析流域不同位置和不同坡面部位土壤微生物生物量碳和氮含量以及土壤侵蚀强度的差异,揭示土壤微生物生物量对土壤侵蚀—沉积的响应规律。结果表明:流域不同位置和不同坡面部位土壤微生物生物量的分布存在明显差异,并呈现出与土壤侵蚀—沉积空间分布相反的变化趋势。土壤侵蚀速率在流域的分布为上游中游下游,在坡面的分布为坡中部坡上部坡下部;土壤微生物生物量碳(Microbial biomass carbon,MBC)和微生物生物量氮(Microbial biomass nitrogen,MBN)在流域表现为下游中游上游,在坡面表现为坡下部坡上部坡中部。回归分析表明,MBC、MBN、有机质(Organic matter,OM)和全氮(Total nitrogen,TN)含量随土壤侵蚀强度的增大而减少。土壤侵蚀对土壤微生物生物量的分布有重要影响,土壤侵蚀—沉积过程引起土壤养分的迁移和再分布是导致侵蚀区和沉积区土壤微生物生物量分布产生差异的重要原因。     

生物质炭对土壤物理性质影响的研究进展

生物质炭在农业与环境中的应用已成为近期国内外研究热点,有关生物质炭特性以及生物质炭对土壤化学、生物学性质和作物产量的影响,已经有一些综述,但是生物质炭对土壤物理性质影响的相关综述很少。本文对近10年生物质炭对土壤物理性质影响相关的研究成果进行了整理分析。研究结果发现生物质炭可以降低土壤容重,提高土壤团聚体稳定性,增加田间持水量和土壤有效水含量,降低饱和导水率等。生物质炭影响土壤物理性质的主要原因是生物质炭具有较大的比表面积和孔隙度。此外,生物质炭与土壤矿质颗粒结合,并通过对土壤微生物活性和植物生长的影响间接影响土壤物理性质。生物质炭对土壤物理性质的影响与多种因素有关,如生物质炭原料、裂解温度、施用量和颗粒大小,土壤质地和处理时间等。关于生物质炭对土壤物理性质影响的长期研究很少,且缺乏田间试验。因此,将来的研究应更加倾向于长期田间条件下生物质炭对土壤物理性质的影响,并逐渐发现生物质炭的作用机理,为实际的农业生产和生态治理提供科学依据。