3种寄生真菌发酵液对大豆胞囊线虫4号生理小种毒力研究

镰孢菌(Fusarium spp.)、厚垣轮枝菌(Verticillium chlamydosporium)和淡紫拟青霉菌(Paecilomyces lilacinus)在连作大豆田中普遍存在。本研究从大豆田土壤中分离了这3个属的真菌,实验室条件下探讨了3个属12个菌株的发酵液对大豆胞囊线虫4号生理小种胞囊、卵孵化抑制作用和对大豆胞囊线虫2龄幼虫的致死作用。结果显示,3个属12个菌株的发酵液对大豆胞囊线虫4号小种都具有抑制作用,抑制其胞囊和卵孵化,并对二龄幼虫具有致死作用。12个菌株发酵原液对胞囊孵化抑制率在50. 6%～85. 6%,对卵孵化抑制率在51. 3%～69. 7%,稀释5倍、10倍、20倍和50倍发酵液也对胞囊孵化和卵孵化也具有抑制作用。12个菌株发酵液对大豆胞囊线虫2龄幼虫均具有致死作用,原液在处理1h就开始出现致死毒性,48 h到72 h致死作用达到高峰。供试菌株中以镰孢菌的F-9菌株、淡紫拟青霉菌的P-E菌株和厚垣轮枝菌V-25菌株发酵液对大豆胞囊线虫抑制作用显著。该结果可为开发大豆胞囊线虫生防菌提供依据。     

航天搭载淡紫拟青霉的生物学效应

为探讨航天诱变对淡紫拟青霉的生物学效应的影响,利用淡紫拟青霉山东菌株搭载"神舟八号"宇宙飞船进行诱变处理。对筛选得到的30个淡紫拟青霉航天诱变菌株进行了形态、色素、菌丝及孢子形态、菌落生长速度、菌丝干重和产孢量等生物学检测,并测定了各诱变菌株对南方根结线虫卵和松材线虫卵的寄生率。结果表明,淡紫拟青霉航天诱变菌株各项生物学特性与原始菌株存在分化。航天诱变后的菌株菌落形态、色素和菌丝结构与原始菌株差异明显;菌落生长速度、菌丝干重和产孢量的负突变率均高于正突变率;菌株对松材线虫卵的致病力较低,寄生率最高的菌株Sd-m-20的寄生率仅为18.0%,菌株对根结线虫卵致病力较高,菌株Sd-m-9,Sd-m-11,Sd-m-16,Sd-m-22和Sd-m-26对根结线虫卵的寄生率分别提高了14.48%、14.90%、12.35%、7.66%和17.45%,显著高于原始菌株的寄生率(78.3%)。航天处理对淡紫拟青霉诱变效果显著,其优良突变菌株可能用于淡紫拟青霉对南方根结线虫的生物防治。     

三种虫生菌协同毒性及与有机肥耦合效应研究

为揭示白僵菌、绿僵菌、淡紫紫孢菌对蚜虫、地蚕、根结线虫的毒性大小,并探索生防菌与有机肥耦合效应,采用PDA对白僵菌、绿僵菌、淡紫紫孢菌对其进行活化培养,并稀释不同浓度菌剂作用于蚜虫,观察单独菌种下毒性大小,然后利用对峙法研究菌种间拮抗性,寻求最优协同杀虫组合,最后利用响应面法设计不同有机肥与菌剂配比,分析混合配方对防效...     

印度梨形孢诱导烟草对黑胫病的抗性及其机理的初步研究

印度梨形孢(Piriformospora indica)是分离自印度塔尔沙漠的植物根系内生真菌,能够诱导多种植物对生物和非生物逆境产生抗性,目前已作为一种模式真菌用于内生真菌与植物互作关系的研究。为了研究印度梨形孢对烟草(Nicotiana tobacum)抗黑胫病的影响,本研究采用烟草疫霉菌(Phytophthora parasitica var.nicotianae)分别接种根部有印度梨形孢定殖与未定殖的烟草,观察接种后根部有印度梨形孢定殖与未定殖的烟草发病情况,测定抗病相关防御酶苯丙氨酸解氨酶(PAL)、过氧化物酶(POD)、多酚氧化酶(PPO)的活性变化及抗病相关基因的表达量,并通过平皿对峙试验研究印度梨形孢对烟草疫霉菌生长的影响。结果表明,接种烟草疫霉菌后,根部有印度梨形孢定殖的烟草发病严重度要显著低于对照;接种烟草疫霉菌后第1~10 d,根部有印度梨形孢定殖的烟草叶片中防御酶PAL、POD和PPO活性高于对照,在第1 d,PAL活性是对照烟草的3.99倍,在第2 d,POD活性是对照烟草的1.5倍,在第4 d,PPO活性是对照烟草的2.66倍;实时荧光定量PCR(Real-time quantitative PCR,q RT-PCR)结果显示,接种烟草疫霉菌后第6~96 h,根部有印度梨形孢定殖的烟草叶片中抗病相关基因PR1b、P450-2、hrs203J、Nm IMSP和P450-1的表达量高于对照;在第12 h,PR1b、P450-2、hrs203J的表达量分别是对照烟草的3.8、15.7和3.1倍,在第48 h,Nm IMSP的表达量是对照烟草的2.6倍,在第72 h,P450-1的表达量是对照烟草的1.5倍;平皿对峙试验结果显示印度梨形孢对烟草疫霉菌的生长没有抑制、重寄生作用。研究结果表明,印度梨形孢可显著提高烟草对黑胫病的抗性,对黑胫病的抗性不是通过印度梨形孢分泌的抗菌物质实现的,是与烟草中抗病相关基因表达量的上升、防御酶活性的提高有关。本研究初步揭示了印度梨形孢诱导烟草抗黑胫病的相关机理,为进一步深入研究烟草黑胫病的生物防治及其机理提供基础资料。     

菌根真菌对3种兰花幼苗生长作用研究

菌根真菌在兰科植物生活史中具有重要作用。为获得促进兰科植物生长的共生真菌,阐明菌根真菌对兰科植物的作用,本研究对从野生建兰中分离获得的3株共生真菌进行形态学和系统进化分析,并将3株真菌与建兰、硬叶兰和铁皮石斛幼苗在不同浓度燕麦琼脂培养基(OMA)条件下共培养,进行比较研究。结果表明,3株真菌分属于胶膜菌属(Tsc)、角担菌属(Cej3)和蜡壳菌属(Sec),且菌丝和菌落特征存在明显差异;系统进化分析发现,Tsc菌株与Cej3菌株亲缘关系较近,与Sec菌株亲缘关系较远;与兰科植物共培养结果表明,Tsc菌株可在全部OMA浓度条件下与3种兰科植物幼苗建立共生关系,且定殖率均为100%;Cej3菌株和Sec菌株与3种兰科植物在不同OMA浓度条件下定殖时间及定殖率存在差异,且具有一定的专一性;与对照组相比,与真菌共生的兰科植物幼苗的平均鲜重、干重、苗高、根长增长率均明显提高。本研究结果为深入研究兰科植物与真菌共生的分子机制奠定了一定的理论基础,并为兰花种质资源的保护提供了参考。     

昆虫病原线虫对烟草根结线虫及蚜虫病虫害以及蚜茧蜂寄生的影响

昆虫病原线虫(EPN)作为一类有潜力的生物防治物质,逐渐受到国内外学者的广泛关注。实验以烟草(Nicotiana tabacum)为研究对象,在人工控制条件下通过外源添加EPN(Steinernema carpocapsae和Heterorhabditis bacteriophora),探究了EPN对烟草地下部根结线虫、地上部烟蚜(Myzus persicae)及烟蚜-蚜茧蜂(Aphidius gifuensis)关系等多重营养级的影响。结果表明:(1)在人工去除烟蚜条件下,施加昆虫病原线虫能显著降低烟草根中根结线虫的卵粒数(P0.05);在有烟蚜的条件下,S.carpocapsae添加处理能显著降低烟草根中根结线虫的卵粒数或者卵粒指数(P0.05);(2)无论EPN添加与否,烟蚜种群生长曲线均很好地符合Logistic增长模型,达到平台期的时间为5~6 d;对照组、S.carpocapsae虫尸剂添加和H.bacteriophora虫尸剂添加这3种条件下,蚜虫种群的环境容纳量分别为89.6、99.8头·株-1和76.6头·株-1,而与之对应的烟草地上部干重分别为0.996、3.258 g·株-1和1.643 g·株-1,这一结果表明EPN添加降低了单位质量烟草叶片上烟蚜的环境容纳量;(3)蚜茧蜂投放早期(2、4、6 d),投放EPN处理组间的僵蚜数目差异显著(P0.05),S.Carpocapsae虫尸剂处理组显著低于H.bacteriophora虫尸剂处理组。研究结果表明,EPN添加可影响烟草地下部根结线虫的繁殖,也可通过"bottom-up"途径影响烟草叶面烟蚜的种群增长以及蚜茧蜂的寄生行为,且这些影响效果与EPN种类有关。     

白花曼陀罗叶提取物对南方根结线虫生长发育的影响

研究了白花曼陀罗(D a tura m etel L inn.)叶提取物对南方根结线虫(M eloid ogyne incogn ita Ch it-w ood.)生长发育的影响。结果表明,白花曼陀罗叶提取物对南方根结线虫胚胎发育和胚后发育有抑制作用。1%白花曼陀罗叶提取物处理48 h,仅有19.88%的卵完成胚胎发育,且变异卵增多;胚后发育历程、初侵染、初产卵日分别推迟2、4、6 d,雌虫产卵量降低。     

生物有机肥对甜瓜根结线虫病的田间防治效果研究

为研究生物有机肥对根结线虫的田间防治效果,在根结线虫发病严重的甜瓜田进行了田间试验。结果表明: 1）在仅施用无机肥的田块,根结线虫病发生严重,而在施用生物有机肥的田块,发病率降低,最高防治率达81.1%; 2）与对照相比,施用加入根结线虫拮抗菌X5的生物有机肥料使地上部鲜重增加,根系鲜重（根结量）减少; 3）施用生物有机肥可显著减少土壤中根结线虫二龄幼虫的密度和根中卵块的数量; 4）生物有机肥处理与常规肥料处理的土壤线虫DGGE图谱明显不同,分别归于两个聚簇,表明土壤线虫群落结构受到施用含根结线虫拮抗菌的生物有机肥影响。综上所述,施用生物有机肥能有效防治田间条件下根结线虫对甜瓜的危害。     

羊肚菌真菌病害调查及分离鉴定

对贵州省龙里县羊肚菌开展真菌病害调查、采集与分离纯化,运用形态学鉴定和rDNA-ITS序列分子检测法构建系统发育树。共分离鉴定了18个真菌病原菌菌株,属于木霉属(Trichoderma)、毛霉属(Mucor)、根霉属(Rhizopus)、镰刀菌属(Fusarium)和阿太菌属(Athelia)等。     

内生菌YN201728的定殖能力及其防治烟草白粉病的效果研究

为研究生防菌YN201728在烟草体内的定殖规律及防病机制,本研究利用其绿色荧光标记菌株YN28-P43GFPmut3a实时动态观测标记菌的定殖部位及密度,并探究其对温室烟草白粉病的盆栽防效与定殖的关系。结果表明,YN28-P43GFPmut3a发酵液处理烟草种子和幼苗后,种子内的标记菌含量可达2.18×10⁶ CFU·g^-1,在幼苗的根表土、根际土、根、茎、叶等组织中均能检测到标记菌,且其定殖密度表现为根表土>根际土>根>茎>叶。激光共聚焦显微镜观察显示,标记菌主要聚集在烟草根表皮、木质部导管、茎表皮、韧皮部及维管束组织、叶片表面和叶肉细胞间隙以及种皮与胚等部位。盆栽防效试验结果表明,YN201728野生型和标记菌株对烟草白粉病均有较好的保护和治疗效果,持效期长达21 d。此外,烟草叶片中内生菌的定殖量与其防效呈正相关。本研究结果表明,内生菌YN201728在烟草体内有良好的定殖和防治白粉病效果,具很好的开发潜力,为烟草病害的生物防治提供了一定的理论基础。     

连作番茄根区病土对番茄生长及土壤线虫与微生物的影响

探索连作番茄根区病土对番茄根结线虫病的诱导效果及引起连作障碍的微生态机制,可为深入了解番茄连作障碍发生机理及探究番茄连作障碍防治方法提供科学依据。本研究利用盆栽试验,测定了番茄在健康土壤及接种病土土壤中生物学特性变化及根结线虫侵染状况,并分析鉴定了土壤中微生物及线虫的种类与数量。结果表明,接种连作番茄根结线虫病株根区病土会对番茄生长及根结线虫侵染产生影响:1)番茄苗期根系根结数达9个?株~(-1),健康土壤无根结;土壤线虫数量较健康土壤增加390.4%;收获期番茄根结线虫侵染率达62.7%,病情指数为80.0%。2)番茄生长受到抑制,叶片防御酶活性降低,收获期茎叶及根系鲜质量较健康土壤分别减少50.2%及33.1%,苗期番茄叶片PPO活性较健康土壤降低15.8%,POD活性较健康土壤增加24.0%,差异均达显著水平(P0.05)。3)番茄根系更易感染有害菌,根系内病原菌甘蓝假单胞菌数量较健康土壤增加463倍,根区土壤细菌、真菌及放线菌总数分别增加46.3%、94.5%及134.0%。4)食细菌线虫、食真菌线虫及植物寄生性线虫数量分别为健康根区土壤的3.3倍、1.6倍及7.3倍,其中的植物寄生线虫95.6%为根结线虫。综上所述,接入连作番茄根结线虫病株根区病土不仅导致番茄遭受根结线虫侵染,而且会导致土壤线虫总量及植物寄生线虫所占比例大幅增加,并使番茄根系内有害细菌数量显著增加,对番茄生长造成显著抑制作用,同时影响番茄的生理生化特性,受线虫侵染番茄防御性酶活性降低,使其更易被根结线虫及病原菌侵染,番茄根区土壤线虫、微生物及根系内优势细菌的种类与数量及其之间的作用发生改变。     

Effect of Inoculation with the Arbuscular Mycorrhizal Fungus Glomus Intraradices on the Root-Knot Nematode Meloidogyne Incognita in Cucumber

ABSTRACT

A pot experiment was carried out to investigate the tolerance of cucumber plants (Cucumis sativus L.) to root-knot nematode after inoculation with Glomus intraradices. Plants were inoculated with G. intraradices for four weeks and then transplanted in soil treated with Meloidogyne incognita for a further five weeks. The low phosphorus (P) loamy soil was amended with 50 and 100 mg P kg^?1 soil. Mycorrhizal colonization increased shoot dry weight, shoot length, leaf numbers, root fresh weight and shoot P concentration, whereas nematode penetration and reproduction were significantly decreased. Similarly, P fertilization usually increased shoot growth and significantly decreased the number of galls and the number of egg masses and eggs per g root. Our results indicate that inoculation with G. intraradices and P fertilizer confer tolerance of cucumber plants to M. incognita by enhancing plant growth and by suppressing reproduction and/or galling of nematodes during the early stages of plant growth.     

Effects of Pseudomonas aeruginosa on the diversity of culturable microfungi and nematodes associated with tomato: impact on root-knot disease and plant growth

The available information on Pseudomonas biocontrol inoculants on the non-target fungal and nematode community is scant. The current paper addresses this issue and investigates the effects of biocontrol agents Pseudomonas aeruginosa IE-6 and IE-6S⁺ (previously shown to suppress several soil-borne plant pathogens) on soil microfungi and plant-parasitic nematodes as well as on the root-knot development and growth of tomato (Lycopersicon esculentum). Furadan, a granular nematicide was included as a treatment for comparative purposes. Treatments were applied to soil at the start of each 52-day-long tomato growth cycle, and their effects on the composition and diversity of rhizosphere and endophytic microfungi and plant-parasitic nematodes were examined at the end of first and fourth growth cycle. Several diversity indices were employed to assess community diversity. A total of 16 genera comprising 23 microfungal species were isolated from the tomato rhizosphere. The most abundant fungal species belonged to the genera Aspergillus, Fusarium, and Penicillium. With a few exceptions, fungi were neither exclusively inhibited nor specifically promoted by the application of treatments at any of the growth cycles studied. However, Paecilomyces lilacinus, an egg and female parasite of root-knot nematode, though exclusively absent in the controls was isolated from the treatments. Both general diversity and equitability of rhizosphere microfungi were greater at first compared to the fourth growth cycle while species richness remained uninfluenced across the growth cycles and treatments. However, Furadan and IE-6S⁺ treatments considerably abated general diversity and equitability. Of the microfungal species isolated from the rhizosphere seven were also recovered from surface-sterilized root tissue of tomato suggesting that all the endophytes are primarily rhizosphere organisms. Diversity of endophytic fungi was consistently lower compared with that of the rhizosphere. Both general diversity and equitability declined in all three treatments relative to controls in the root tissue but species richness remained unaltered. Diversity and equitability of plant-parasitic nematodes in soil were reduced by all three treatments over the controls at fourth growth cycle whilst species richness did not change at either growth cycle. The biocontrol agents significantly reduced root-knot development and enhanced shoot growth of tomato over the controls. The possible implications of fungal composition and abundance because of biocontrol by Pseudomonas application are discussed.     

Response to root-knot nematodes of a germplasm collection of red clover and related species

A germplasm collection of red clover (Trifolium pratense L.) and seven related species was evaluated in greenhouse tests for resistance to Meloidogyne arenaria (Neal) Chitwood, M. hapla Chitwood, M. incognita (Kofoid & White) Chitwood, and M. Javanica (Treub) Chitwood. Plants were rated for root galling severity and nematode egg production at eight weeks after inoculation with 1500 nematode eggs. A resistance index, RI = (gall₂; + egg₂;), was generated to assess the plants' reaction to nematode infection as immune, highly resistant, resistant, moderately resistant, intermediate, moderately susceptible, susceptible, or highly susceptible. More than 98% of red clover accessions were intermediately to highly susceptible to all four root-knot nematode species. Only one accession, PI 271627 introduced from India, had a moderate resistance level to the four nematodes tested. About one third of the T. medium accessions were resistant or highly resistant to M. arenaria, M. incognita, and M. javanica whereas more than 50% of the T. alpestre accessions were highly resistant or immune to all four nematodes species. Genetic factors for resistance to root-knot nematode could be introduced into T. pratense through interspecific hybridization with T. medium and/or T. alpestre     

Effectiveness of native West African arbuscular mycorrhizal fungi in protecting vegetable crops against root-knot nematodes

Twenty strains of arbuscular mycorrhizal fungi (AMF), native to West Africa, and three commercial AMF, were evaluated for their protective effect against root-knot nematodes, Meloidogyne spp., in pots and field experiments in Benin. In pots, these strains were assessed in sterilized soil following inoculation of nematodes and in non-sterilized soil naturally infested with nematodes using tomato. The four strains showing greatest potential in suppressing nematode development were further assessed in the field with a relatively high natural infestation level of nematodes (155 per 100 cm³ soil) over a tomato–carrot double cropping. In the pot experiments, most native strains provided significant suppression of nematode multiplication and root galling, but in most cases the level of nematode control depends on either sterilized or non-sterilized soils. In the field experiments, application of AMF mostly resulted in significant suppression of nematode multiplication and root galling damage on both crops indicating that the AMF persists and remains protective against root-knot nematodes over two crop cycles. Field application of AMF increased tomato yields by 26% and carrot yields by over 300% compared with the non-AMF control treatments. This study demonstrates for the first time, the protective effect of indigenous West African AMF against root-knot nematodes on vegetables. The potential benefits of developing non-pesticide AMF-based pest management options for the intensive urban vegetable systems are evident.     

Pre-inoculation with arbuscular mycorrhizal fungi suppresses root knot nematode (<Emphasis Type="Italic">Meloidogyne incognita</Emphasis>) on cucumber (<Emphasis Type="Italic">Cucumis sativus</Emphasis>)

A pot experiment was conducted to evaluate the influence of pre-inoculation of cucumber plants with each of the three arbuscular mycorrhizal (AM) fungi Glomus intraradices, Glomus mosseae, and Glomus versiforme on reproduction of the root knot nematode Meloidogyne incognita. All three AM fungi tested significantly reduced the root galling index, which is the percentage of total roots forming galls. Numbers of galls per root system were significantly reduced only in the G. intraradices + M. incognita treatment. The number of eggs per root system was significantly decreased by AM fungus inoculation, no significant difference among the three AM fungal isolates. AM inoculation substantially decreased the number of females, the number of eggs g⁻¹ root and of the number of eggs per egg mass. The number of egg masses g⁻¹ root was greatly reduced by inoculation with G. mosseae or G. versiforme. By considering plant growth, nutrient uptake, and the suppression of M. incognita together, G. mosseae and G. versiforme were more effective than G. intraradices.     

Host-status of sweet potato cultivars to South Africa root-knot nematodes

Globally, most of the crop-related developmental projects collapsed due to the existence of aggressive plant-parasitic nematodes. Intensive South African biofortification efforts using exotic and local sweet potato (Ipomoea batatas) cultivars have hardly considered the inclusion of nematode resistance in plant breeding, despite the withdrawal of most nematicides from agrochemical markets. The objective of this study was to screen 12 selected biofortification sweet potato cultivars for host-status to South Africa tropical root-knot (Meloidogyne species) nematodes. Three exotic orange-fleshed, three local orange-fleshed and six cream-fleshed cultivars were used in three separate experiments of M. javanica and races 2 and 4 of M. incognita. In each experiment, 6000 eggs and second-stage juveniles/plant were used for inoculation, with nematode assessment performed at 56 days after inoculation. All tested biofortification exotic (except for cv. ‘W-119’) and local cultivars were hosts to Meloidogyne species and races, whereas three non-biofortication local cultivars, namely, ‘Bosbok’, ‘Blesbok’ and ‘Mvuvhelo’ were non-hosts. In conclusion, the findings suggested the likelihood of the existence of non-host-status in certain exotic and local sweet potato cultivars for use in plant breeding programmes against South African Meloidogyne species and races.     

Genetic diversity for root-knot nematode resistance in white clover and related species

A total of 237 Plant Introduction in eleven Trifolium species were evaluated for resistance to Meloidogyne arenaria (Neal) Chitwood race 1, M. hapla Chitwood, M. incognita (Kofoid & White) Chitwood race 3, and M. javanica (Treub) Chitwood. Plants were infected with 1500 nematode eggs collected from 'Rutgers' tomato (Lycoperiscon esculentum Mill.) roots with 0.5% NaOCl. Ratings of galling severity and egg mass production were assigned to each plant 8 wk after inoculation. Host plant reaction was classified as immune, highly resistant, resistant, moderately resistant, intermediate, moderately susceptible, susceptible, and highly susceptible according to the resistance index . More than 95% of 171 white clover accessions were moderately to highly susceptible to all four nematodes species. The best white clover accessions were only moderately resistant to either M. arenaria (PI 291843 and PI 306286) or M. hapla (PI 100250 and PI 204930). Accessions with moderate resistance or resistance to root-knot nematodes were found among relatives of white clover, with T. ambiguum M. Bieb. exhibiting the greatest resistance level. Among the other Trifolium species evaluated, T. carolinianum Michx. PI 516273 was immune or highly resistant to all four nematode species while accessions of T. hirtum All. showed a wide range of reaction to root-knot nematodes. Identified germplasm of white clover relatives with resistance to root knot nematodes should be useful for the selection of parents in white clover breeding programs.     

水稻-番茄轮作植物寄生线虫及微生物群落结构差异

土壤线虫和微生物群落结构与作物健康密切相关。采用田间试验，利用传统分类和高通量测序技术，研究了水稻–番茄轮作(FS)、番茄连作(FF)和休耕(CK)3种种植制度土壤线虫及微生物群落结构差异。结果表明，水旱轮作土壤中线虫种类少，自由生活线虫占比高。土壤中植物寄生线虫属的检出率，轮作最少，仅有5个，连作最多，共13个，且大部分为常见属；水旱轮作同时能降低土壤中植物寄生线虫的种类，包括主要危害番茄的根结线虫属，占比仅2.1%，而连作为29.7%。不同种植制度下真菌和细菌类群OUTs和群落结构差异明显；与连作相比，轮作和休耕真菌、细菌种群丰富度更高，共享占比更多，群落构成更相近；水旱轮作Alpha多样性指数显著高于连作；轮作土壤中植物寄生线虫拮抗微生物类群Chaetomium,Talaromyces,Anaerolineaceae和Acidobacteria相对丰度高于连作。水稻–番茄轮作能大幅减少植物寄生线虫种类，增加微生物群落多样性，改善土壤质量，是番茄生产中防控线虫病害的理想措施。     

Effect of the arbuscular mycorrhizal fungus <Emphasis Type="BoldItalic">Glomus intraradices</Emphasis> on the false root-knot nematode <Emphasis Type="BoldItalic">Nacobbus aberrans</Emphasis> in tomato plants

Information about the interaction between arbuscular mycorrhizal fungi (AMF) and the false root-knot nematode Nacobbus aberrans (Thorne, 1935) Thorne & Allen, 1944 is scarce. The effect of Glomus intraradices Schenk & Smith on tomato (Lycopersicon esculentum L.) cv. Platense inoculated with nematode juveniles from Lisandro Olmos (Argentina) was studied under greenhouse conditions. Six treatments with five replications were performed. After 80 days, nematode reproduction and percentage of AMF colonization in roots were estimated. Some plant growth parameters were also measured. In general, plants with AMF and AMF plus nematodes grew as well as the control without AMF and without nematodes. Furthermore, G. intraradices was beneficial in reducing nematode-induced damage in roots (lower number of galls) as well as in having a suppressive effect on parasite reproduction. This is the first study on the use of G. intraradices as a possible strategy in the control of N. aberrans in tomato.