Effects of dietary protein and energy concentrations on performance and carcase characteristics of chukar partridge (Alectoris chukar) raised in captivity

1. This study was conducted to determine the effects of starter and grower diets with differing crude protein (CP) and metabolisable energy (ME) concentrations on the body weight (BW), live weight gain (LWG), feed consumption (FC), feed conversion ratio (FCR), and carcase, breast+back, rump, wing, neck and abdominal fat weights of chukar partridge raised in captivity. 2. Chukar partridges were fed on starter diets containing 4 concentrations of CP (160, 200, 240, 280 g/kg) and 4 concentrations of ME (10.9, 11.7, 12.6, 13.4 MJ/kg) from hatch to 8 weeks of age; they were fed on grower diets containing 4 concentrations of CP (150, 175, 200, 225 g/kg) and 4 concentrations of ME (11.9, 12.6, 13.2, 13.8 MJ/kg) from 9 to 16 weeks of age. All diets contained at least 5.5 g/kg methionine, 15 g/kg lysine and 10 g/kg methionine+cystine. Sixteen starter and 16 grower diets were arranged in a 4 x 4 factorial design with 4 levels of CP and 4 levels of ME. Each treatment was replicated three times with each replicate consisting of 5 males and 5 females. 3. Partridges fed on a starter diet containing 160 g CP/kg were significantly lighter at 8 weeks of age than those in groups given diets containing a higher CP. However, at 16 weeks of age, the differences in BW among treatments had disappeared. Throughout, there were no significant effects of ME concentration on BW and LWG. 4. The daily mean FC for the 0 to 8 week and 0 to 16 week periods was not affected by dietary CP concentration. For the 9 to 16 week period, the partridges fed on a grower diet containing 225 g CP/kg consumed more feed than those given a diet containing 175 g CP/kg. 5. The highest FCR for the 0 to 8 week period was in partridges fed on a starter diet containing 160 g CP/kg. For the 9 to 16 week period, the lowest FCR was in partridges fed on a grower diet containing 150 g CP/kg. For the 0 to 16 week period, there was not a significant effect of dietary CP concentration on FCR. The daily mean FC and the FCR for the 0 to 8, 9 to 16 and 0 to 16 week periods decreased when the ME concentration of the starter and grower diets increased. 6. The carcase, rump and breast+back weights of the male partridges increased when the ME content of the diets increased. Weights of all carcase components of the male partridges were significantly greater than those of the carcase components of the females. 7. There were no significant interactions between CP and ME concentrations on BW, LWG, FC, FCR and carcase characteristics. 8. We conclude that the starter diet for chukar partridges raised for meat production should contain at least 200 g CP/kg, 11.7 MJ ME/kg, and the grower diet should contain 150 g CP/kg, 12.6 MJ ME/kg.     

Effects of sex and protein and energy levels in the diet on the blood parameters of the chukar partridge (Alectoris chukar)

1. The effects of sex and dietary composition were investigated in 48 male and 48 female 16-week old chukar partridges. 2. Sixteen starter and 16 grower diets were arranged in a 4 x 4 factorial design with 4 concentration of crude protein (CP) and 4 concentration of metabolizable energy (ME). 3. Blood samples were collected at 16 weeks of age and analysed for total protein, triglycerides, uric acid, total cholesterol, glucose, calcium, phosphorus, sodium, potassium and chlorine. 4. There were no significant effects of sex on the measured parameters. 5. Serum total protein was highest in partridges fed on a diet containing 200/175 g CP/kg (starter grower). Serum cholesterol concentrations of partridge fed the diet with 240/200 or 280/225 g CP/kg were significantly higher than that for partridge fed diets containing a lower gCP/kg. 6. Serum total protein and glucose levels significantly decreased as dietary energy level was increased. Serum triglycerides and calcium were highest in partridge fed on the diets containing 13.39/13.81 and 11.71/12.55 ME MJ/kg, respectively. Serum phosphorus and chloride levels were highest in partridges given the diet containing 10.88/11.92 ME MJ/kg. 7. There were significant interactions between the effects of CP and ME on serum triglycerides, sodium and potassium levels. 8 The results of this study suggested that dietary CP, and especially ME concentration, significantly affect blood parameters in chukar partridge.     

Use of industrial amino acids to allow low protein concentrations in finishing diets for growing Muscovy ducks

1. Three experiments were performed to assess the effects of decreasing protein concentration in the finishing diets for growing Muscovy ducks (8 to 12 weeks of age) by adding 4 essential amino acids (AAs, lysine, methionine, threonine and tryptophan). Experimental diets with crude protein (CP) contents from 105 to 142 g/kg, were compared with control diets providing 150 to 160 g/kg CP. In each trial, all diets were isocaloric. No significant modification in growth or carcase quality was observed when CP was greater than 124 g/kg in diets supplemented with the 4 essential AAs. 2. There was no advantage in supplying more than 4.3 g of digestible lysine per kg of diet (12.75 MJ ME/kg). When threonine was not added, breast yield decreased significantly (- 4.3%), while omitting tryptophan supplementation did not influence performance. 3. Furthermore, the experiments confirmed that reducing CP had little or no effect on food conversion efficiency and fatness in Muscovy ducklings, unlike the observed situation in broiler chickens.     

The use of low‐protein diets in the 12‐ to 18‐week period of light hybrid pullets

Three diets, containing nearly 12.5 MJ/kg (3.0 kcal ME/g) and 14.6, 11.3 and 9.1 % crude protein, were fed to 1680 light‐hybrids from 12 to 18 weeks of age. Body weight at 18 weeks was depressed by 5 and 9% and age at sexual maturity delayed by 2.4 d and 2.8 d for groups fed the intermediate and lowest protein diets respectively. Mortality during the experiment was slightly higher in the group receiving the higher protein diet. There were no consistent or significant treatment differences in mortality during the laying period. Both hen‐day rate of lay and egg weight decreased slightly and insignificantly but progressively with decreasing protein level of diets fed in the 12‐ to 18‐week period. Savings in food costs in the 12‐ to 18‐week period from feeding the cheaper lower‐protein diets were more than offset by the reductions in egg output and returns which resulted.     

High-fibre pelleted rations decrease water intake but do not improve physiological indexes of welfare in food-restricted female broiler breeders

1. A 3x2 factorial experiment was conducted with three diets and two lines of broiler breeder females to evaluate the contribution of low-energy rations for improving the welfare of feed-restricted birds during rearing. Experimental diets were fed from 6 to 16 weeks of age and were created by diluting a conventional grower (Control) ration containing 11.0 MJ ME/kg with 200 (8.8 MJ ME/kg) or 400 (6.6 MJ ME/kg) g oat hulls/kg using Optimoist to facilitate the pelleting process. Welfare was assessed by changes in behaviour and physiological variables at 8, 12 and 16 weeks of age. Birds were fed restricted quantities of feed to meet recommended body weight targets. 2. There was a decrease in the proportion of observations of drinking and an increase of preening in birds fed on the two experimental diets compared with the control. There was a linear decrease in litter moisture and the number of litter changes with increasing diet dilution, and water intake at 12 weeks was higher in the control than in the two experimental diets. There were no changes in physiological indexes of welfare (heterophil-lymphocyte ratio, plasma corticosterone and antibody responses) associated with the dietary treatments. 3. There were no important differences in the growth, behaviour or physiological responses to dietary treatment between the two lines of broiler breeders. Changes with age were similar to those reported in other experiments. 4. It was concluded that low-energy pelleted diets would improve litter conditions but not improve indexes of welfare in feed-restricted broiler breeders.     

The effect of dietary energy and protein levels on production in breeding female ostriches

1. In a study spanning two breeding seasons, we assessed the effect of different dietary energy and protein levels on body mass, body condition, and egg production of female ostriches. 2. During the first breeding season, groups were given diets with energy concentrations of 8.5, 9.5 and 10.5 MJ/kg dry mass (DM) metabolisable energy (ME) and protein concentrations of 135, 150 and 165 g/kg. In the second breeding season, groups were given diets with ME of 7.5, 8.5 and 9.5 MJ/kg and protein contents of 105, 120 and 135 g/kg. 3. Body mass of birds on diets of 7.5 and 8.5 MJ/kg ME decreased significantly in the course of the breeding season compared with birds fed on diets with higher energy contents and body measurements decreased, suggesting a loss of body condition. 4. Females fed on diets containing only 7.5 MJ/kg ME produced significantly fewer eggs at significantly longer intervals, resulting in fewer chicks hatched. 5. There was no significant difference in egg mass, initial chick mass, chick survival to one month of age and body mass of chicks at one month. 6. Dietary protein concentrations had no effect on egg production, egg mass, hatchability, initial chick mass, chick survival or chick mass at one month old. 7. The female ostriches regained their original body mass during the 4-month rest period between breeding seasons, but significant differences in some parameters during the second breeding season suggest that they may not have fully recovered their body condition. 8. A dietary energy content of 7.5 MJ/kg proved to have an adverse effect on egg production by breeding female ostriches, and it may be concluded from this study that a diet containing 8.5 MJ ME/kg DM and 105 g/kg protein should be regarded as the minimum that can be used for breeding female ostriches without compromising egg production.     

Influence of dietary protein and sex on walking ability and bone parameters of broilers

1. The study was conducted to investigate the effect of dietary protein on the walking ability and bone parameters of broilers reared under summer temperatures which ranged from 26 to 32C(+/-2C). 2. Three different dietary protein combinations were used. The diets (per kg) were: low protein with 205 g crude protein and 12.94 MJ ME, 184 g crude protein and 12.75 MJ ME; medium protein with 219 g crude protein and 12.99 MJ ME, 201 g crude protein and 12.87 MJ ME; and high protein with 238 g crude protein and 12.99 MJ ME, 216 g crude protein and 12.96 MJ ME from 0 to 4 and 4 to 7 weeks of age, respectively. Body weights of birds were recorded and birds' walking ability (gait scoring) were scored for each bird, according to 3 categories (completely normal to immobile, at 4 and 7 weeks). Tibia parameters and tibia plateau angles were also determined at 7 weeks. 3. Birds fed on the low protein were lighter than those fed on the medium or high protein diets. At 7 weeks, birds with poor walking ability weighed 149 g less than birds with no walking difficulty. 4. Bone parameters were not affected by dietary protein, sex or gait score. There was a significantly positive correlation between bone strength and radiographic density. Bone strength was also significantly correlated with bone weight and length.     

Food intake and abdominal adipose tissue in White Leghorn hens fed diets of different protein and energy concentrations

1. Single Comb White Leghorn hens of 2 ages (44 and 80 weeks) were fed diets of different energy (10.88, 12.13 or 13.39 MJ ME and 140 g protein/kg) or protein (120, 140, 160 or 180 g and 12.13 MJ ME/kg) concentration over an 8-week period. 2. Food intake did not change with increasing concentrations of dietary protein. Protein intake was directly correlated with dietary protein concentration. 3. Energy intake increased with dietary energy concentration but, generally, failed to match the increases in dietary energy concentration. Energy, rather than protein, concentration was the major determinant of food intake. 4. Efficiency of energy utilisation decreased and mean adipocyte size increased with higher energy intake. 5. A bimodal adipocyte size distribution, consisting of a primary large size and a secondary small size population, was present in the abdominal fat pad of birds of both ages. There was no significant difference in the numbers of large adipocytes between the hens of the two ages. 6. The greater mean fat pad weight in the older hens was associated with increased mean cell volume in the population of large adipocytes.     

Effects of probiotic Bacillus cereus toyoi on abdominal fat accumulation in the Japanese quail (Coturnix japonica)

Effects of a commercial probiotic, Bacillus cereus toyoi on abdominal fat accumulation in the Japanese quail fed either a commercial control diet (crude protein (CP), 23.5%; metabolizable energy (ME), 11.7MJ/kg) or a high‐energy diet (CP, 23.0%; ME, 13.8MJ/kg) were investigated. Four‐week‐old male birds (n = 108) were divided into four diet groups: control diet, control with probiotic, high‐energy diet and high‐energy diet with probiotic. At 8 weeks and 12 weeks of age (4 and 8 weeks of probiotic supplementation period, respectively), abdominal fat, muscles and the liver were weighed. The weight of total fat and protein in the muscles and liver were also determined. A rectal temperature was recorded weekly. Bodyweight and feed intake in both diets were not affected by probiotic supplementation. At 8 weeks of age, birds fed the control diet with probiotic had significantly less abdominal fat than those fed without the probiotic, and a similar tendency was seen for both diet groups at 12 weeks of age. The decrease in abdominal fat coincided with an increase in rectal temperature. These results suggest that greater metabolizable energy was consumed through elevation of heat production by supplementation of B. cereus toyoi, with hardly any energy stored as body fat. Probiotic supplementation appears to induce greater muscle weight, higher protein and lower lipid content in muscles by 8 weeks of age, and lower lipid content in muscles and greater liver weight by 12 weeks of age, in the Japanese quail.     

5～8周龄固始鸡能量和蛋白质需要量的研究

本试验选用 4周龄固始肉鸡 972只 (公母各半 )。采用 3× 3因子设计 ,饲喂代谢能 (ME)和粗蛋白质 (CP)水平不同的 9种玉米—鱼粉—豆粕型日粮。通过饲养试验、比较屠宰试验和代谢试验 ,研究不同的能量和粗蛋白质水平对 5～ 8周龄的固始鸡雏鸡生产性能、屠体成分的影响 ,根据三个试验的研究结果 ,得出固始鸡在 5～ 8周龄阶段 ,其日粮适宜的能量和粗蛋白质营养水平分别为 :代谢能 12 .6 9MJ Kg,粗蛋白质 18.2 0 % ,蛋能比 14 .34g MJ。     

Prediction of ingredient quality and the effect of a combination of xylanase, amylase, protease and phytase in the diets of broiler chicks. 2. Energy and nutrient utilisation

1. In order to investigate the effects of xylanase, amylase, protease and phytase in the diets of broiler chickens containing graded concentrations of metabolisable energy (ME), two 42-d experiments were conducted using a total of 2208 broiler chicks (8 treatments with 12 replicate pens in each experiment). 2. Four diets including one positive and three negative control diets were used. Three maize/soybean meal-based negative control (NC) diets were formulated to be identical in available phosphorus (P), calcium (Ca) and amino acids but NC1 contained approximately 0.17 MJ/kg less ME than NC2 and approximately 0.34 MJ/kg less ME than NC3. A positive control (PC) was fed for comparison and was formulated to be adequate in all nutrients, providing approximately 0.63 MJ/kg ME, 0.13% available P, 0.12% Ca and 1 to 2% amino acids more than NC1. 3. The reduction in nutrient density between NC1 and PC was determined using ingredient quality models Avichecktrade mark Corn and Phychecktrade mark that can predict the response to exogenous enzymes in maize/soybean meal-based broiler diets. Supplementation of each diet with or without a cocktail of xylanase, amylase, protease and phytase gave a total of 8 dietary treatments in a 4 x 2 factorial arrangement. The same treatments and diet designs were used in both experiments but conducted in different locations using different batches of maize, soybean meal and minor ingredients. 4. In both experiments, digestibility was improved by the addition of exogenous enzymes, particularly those for P, Ca and certain amino acids. In addition, the supplementation of the PC with enzymes elicited a positive response indicating that over-the-top addition of xylanase, amylase, protease and phytase may offer a nutritionally and economically viable alternative to feed cost reduction. 5. It can be concluded that the digestibility of nutrients by broilers fed on maize/soybean meal-based diets can be improved by the use of a combination of xylanase, amylase, protease and phytase.     

半细毛羊5种玉米的可消化粗蛋白质和有效能的测定及其预测

本试验旨在采用概略养分分析法测定5种玉米的营养成分含量以及通过消化代谢试验实测玉米的可消化粗蛋白质含量和有效能值,并利用先采集的4种玉米(奥美特、杰尼336、巨丰66、京科665)数据拟合可消化粗蛋白质、消化能和代谢能分别与营养成分之间的回归方程,然后利用第5种玉米(禾育九)可消化粗蛋白质和有效能数据来对回归方程进行验证和修正。选取16只体重为(56.44±5.11)kg的云南半细毛羊,采用完全随机设计,平均分为4组,每组4只。试验共分2期进行,共6种日粮,分别是基础日粮和5种试验日粮,第1期饲喂4种日粮,第2期饲喂2种日粮。结果表明:京科665的可消化粗蛋白质(DCP)、消化能(DE)和代谢能(ME)含量最高,杰尼336的DCP、DE和ME含量最低;玉米的DE和ME分别与EE呈极显著负相关关系(P<0.01),相关系数分别为-0.985和-0.994;试验所得的预测方程为DCP(g/kg)=258.961-52.624EE(粗脂肪)、DE(MJ/kg)=40.506-6.876EE、DE(MJ/kg)=34.677-6.398EE+0.492CP(粗蛋白质)、DE(MJ/kg)=-115.182-6.572EE+0.473CP+1.525OM(有机物)、ME(MJ/kg)=-1.028+0.945DE、ME(MJ/kg)=2.950+0.916DE-1.060CF(粗纤维)和ME(MJ/kg)=37.586-6.586EE。综合DCP含量和有效能值,京科665的品质最佳,其次是巨丰66、奥美特和禾育九,杰尼336品质最差;以半细毛羊为试验对象得出,可以利用玉米的营养成分预测其DCP含量以及有效能值。     

Dietary thyroid hormone improves growth and muscle protein accumulation of black-boned chickens

1. The objective of the study was to examine the effect of dietary thyroid hormone on performance and muscle protein accumulation of black-boned chickens. 2. A total of 720 1-d-old birds was housed in 24 pens and assigned to 4 diets containing triiodothyronine (T3) at 0, 0.1, 0.3 or 0.5 mg/kg. 3. The trial was split into starter (0 to 4 weeks) and grower (5 to 8 weeks) phases. At the beginning of the grower phase, each pen was split into two; one subgroup was fed basal diet (T3-) and the other continued with T3-added diet (T3+). 4. Dietary T3 at 0.1 mg/kg improved average daily body weight gain until week 6 but retarded it thereafter. However, T3 at 0.5 mg/kg depressed growth throughout the whole period. During weeks 7 to 8, T3 retarded growth, whereas growth recovered when T3 was withdrawn from the diet. 5. Birds fed the diet containing 0.1 mg/kg during weeks 0 to 4 and those receiving basal diet during weeks 5 to 8 showed the greatest thigh muscle growth, but this growth was depressed when they were fed the 0.5 mg/kg diet during weeks 0 to 8. 6. Serum T3 concentration was increased, while thyroxine (T4) concentration was decreased by dietary T3 treatment at 2 weeks old. At 4 weeks old, T3 and T4 concentrations decreased. At 6 and 8 weeks old, among T3-treated groups the serum T3 concentration of the T3- group was higher than that of the T3+ group. 7. In conclusion, dietary T3 improved weight gain and muscle growth of black-boned chickens before week 6, whereas supplementation depressed growth after 6 weeks. Optimum dietary T3 concentrations showed a carry-over effect on both body weight gain and thigh muscle growth.     

Feeding motivation and plasma metabolites in pregnant sows fed diets rich in dietary fiber either once or twice daily

The present study investigated the effects of source and level of dietary fiber (DF) and feeding frequency (once vs. twice daily) on feeding motivation and plasma metabolites at 4 different time points post feeding. Sixty pregnant sows (Sus scrofa, 4 blocks of 15 sows) were allocated to 1 of 5 diets within blocks. Four diets were restricted (approximately 35 MJ ME/d): a barley and wheat control diet (171 g DF/kg DM; 12 g DF/MJ ME), and 3 fiber diets formulated to contain 35% DF by including pectin residue (323 g DF/kg DM; 25 g DF/MJ ME), potato pulp (404 g DF/kg DM; 29 g DF/MJ ME), or sugar beet pulp (367 g DF/kg DM; 25 g DF/MJ ME). The fifth diet was a mixture including an equal amount of the 3 fiber diets offered semi ad libitum (ad libitum access to feed during 6 periods of 1 h starting at 0300, 0600, 1100, 1500, 1800, and 2300; 354 g DF/kg DM; 25 g DF/MJ ME). The experimental period included 2 periods of 4 wk each. Restricted-fed sows were fed once daily (0800 h) during the first period and twice daily (0800 and 1500 h) during the second period, or vice versa. Semi ad libitum fed sows had access to feed 6 times a day in both periods. In each period, the feeding motivation was assessed in an operant conditioning test, and samples of peripheral blood were taken in a balanced design, at 0900, 1200, 1900, and 0700 h, corresponding to 1, 4, 11, and 23 h after feeding for restricted sows fed once daily. No differences in the feeding motivation were found between the 4 restricted diets at any of the time points post feeding, but semi ad libitum fed sows had a decreased feeding motivation (P < 0.001). Among the restricted-fed sows, feeding twice daily resulted in decreased feeding motivation at 1900 h (P < 0.001) and at 0700h (P < 0.05) compared with feeding once daily, but not at 0900 and 1200 h, indicating that feeding twice daily reduced feeding motivation during the night compared with feeding once daily. Among restricted-fed sows, plasma concentrations of short-chain fatty acids (SCFA) were greater in sows fed high-fiber diets compared with the control (P = 0.02). Nonesterified fatty acid was least in sows on the control diet and greatest in sows on the potato diet, whereas sows on the pectin and sugar beet diets were intermediate (P < 0.001). Less diurnal variation in glucose (P < 0.001) was seen in sows on high-fiber diets. In spite of the found effects on plasma metabolites, the applied level of fiber in the diet of restrictedly fed sows did not reduce their feeding motivation irrespective of fiber source.     

Dietary methionine requirement of the Chinese egg-laying duck

1. The dietary methionine requirement of egg-laying ducks was assessed by feeding diets supplemented with graded levels of DL-methionine (0, 4, 8, 12, 16 g/kg dietary protein) for 8 weeks. The basal diet contained 175 g protein and 2.6 g methionine per kg feed (or 14.9 g/kg protein) and an estimated ME of 11.5 MJ/kg. 2. A total of 800 Shaoxin laying ducks (420 d old) were randomly divided into 5 groups of 160 each and fed in 4 separate pens. 3. Dietary supplementation of methionine significantly increased egg production and feed conversion efficiency. 4. Dietary methionine requirement for optimum egg production was estimated to be 25.7 g/kg of dietary protein or 4.5 g/kg of the diet or 380 mg/bird-d. 5. Methionine supplementation increased the methionine level in plasma, and the free glutamic acid and aspartic acid concentrations in plasma were quadratically related to dietary methionine levels. Increasing dietary methionine had little effect on egg quality characteristics.     

不同日粮对广西合浦鹅生产性能及养分代谢的影响

本研究旨在探讨不同日粮对鹅生长性能和纤维类物质及其他养分利用能力的影响。选取4周龄、体重相近的广西合浦鹅60只,随机分为3组,每组20只(公母各1/2),分别饲喂3种不同代谢能(ME)水平的日粮:第1组10.03 MJ/kg,第2组11.00 MJ/kg,第3组11.85 MJ/kg,进行42 d的饲养试验;同时分别于5周龄和9周龄时从上述3组试验鹅中各选取4只体重相近的试鹅进行代谢试验并用内源指示剂法(4 mol/L HCl不溶灰分)测定试鹅对日粮养分的代谢率。结果表明:在整个试验期间各组试鹅平均日增重差异不显著(P>0.05);5周龄时,各组日粮养分代谢率差异也不显著(P>0.05);但在9周龄时,第1组的CF、DM和能量代谢率显著高于其余2组(P<0.05或P<0.01)。因此,本试验证明了日粮ME在10.03~11.85 MJ/kg时,不影响合浦鹅的增重和屠宰性能;在9周龄、日粮ME为10.03 MJ/kg时,其CF、DM和能量代谢率显著高于ME为11.00或11.85 MJ/kg组,而高能量日粮并未表现出养分代谢上的优势。     

20～50 kg川藏黑猪的能量代谢与沉积规律研究

本试验旨在研究20~50 kg川藏黑猪的能量代谢与沉积规律。试验一:选择体重接近[(20.17±3.46)kg]的川藏黑猪配套系商品猪64头,预试7 d后屠宰4头猪测定胴体成分;其余试验猪按公母随机分成5个组,每个组4个重复,每个重复3头,各重复单圈饲养,自由采食消化能(DE)水平分别为13.79、13.37、12.96、12.54和12.12 MJ/kg的饲粮。试验猪体重达50 kg时,结束试验一,测定平均日采食量、平均日增重和料重比,并在各组选择1头猪屠宰测定胴体成分。试验二:选择试验一中15头体重接近[(48.34±4.07)kg]的公猪,随机分成5个组,每个组3个重复,每个重复1头,单独饲养于代谢笼,分别采食上述5种DE水平的饲粮,进行消化代谢试验。预试期3 d,正试期4 d。采用析因法建立能量需要量预测模型。结果表明:饲粮DE水平影响了20~50 kg川藏黑猪的平均日采食量、平均日增重和料重比;饲粮DE转化为代谢能(ME)效率(ME/DE)为97.26%~98.10%,ME用于沉积产品能的效率(DED/ME)平均值为41.71%;此阶段川藏黑猪维持需要ME平均值为0.49 MJ/W~(0.75)或0.85 MJ/W~(0.60)(按DE计为0.50 MJ/W~(0.75)或0.87 MJ/W~(0.60)),增重需要DE和ME平均值分别为18.91和18.47 MJ/kg。由此得出,20~50 kg阶段川藏黑猪能量需要模型为:DE(MJ/d)=0.504 W~(0.75)+18.91ΔW或DE(MJ/d)=0.867 W~(0.60)+18.91ΔW;ME(MJ/d)=0.492 W~(0.75)+18.47ΔW或ME(MJ/d)=     

饲粮不同代谢能、粗蛋白质水平对新杨绿壳蛋鸡产蛋后期生产性能、蛋品质及血清生化指标的影响

本试验旨在通过研究饲粮不同代谢能、粗蛋白质水平对新杨绿壳蛋鸡产蛋后期(44~56周龄)生产性能、蛋品质及血清生化指标的影响,得出其产蛋后期饲粮代谢能和粗蛋白质的适宜配比。选取768羽体重相近的43周龄末新杨绿壳蛋鸡,随机分为6组,每组4个重复,每个重复32羽。采用2×3因子(代谢能水平为10.87和11.08 MJ/kg,粗蛋白质水平为15.00%、15.50%和16.00%)设计试验,共设计6种饲粮。试验预试期1周,正试期12周。结果表明:1)饲粮不同代谢能、粗蛋白质水平对绿壳蛋鸡产蛋后期产蛋率和料蛋比有显著影响(P0.05),饲粮代谢能和粗蛋白质水平在产蛋率上表现出极显著的交互作用(P0.01),其中,高能低蛋组(代谢能为11.08 MJ/kg,粗蛋白质为15.00%)产蛋率最高,料蛋比最低。2)饲粮较高粗蛋白质水平(16.00%)能显著提高绿壳蛋鸡产蛋后期鸡蛋的哈氏单位(P0.05);饲粮较高代谢能水平(11.08 MJ/kg)能显著提高绿壳蛋鸡产蛋后期鸡蛋的蛋黄颜色(P0.05);饲粮代谢能和粗蛋白质水平在蛋品质上未表现出显著的交互作用(P0.05)。3)高能低蛋组绿壳蛋鸡血清尿素氮含量最低;饲粮较高代谢能水平(11.08 MJ/kg)能显著降低绿壳蛋鸡血清总胆固醇含量(P0.05)。综上,在本试验条件下,饲粮代谢能、粗蛋白质水平分别为11.08 MJ/kg和15.00%时,更有利于绿壳蛋鸡产蛋后期生产性能的发挥和蛋品质的改善。     

不同代谢能和粗蛋白质水平对合浦鹅日粮养分消化率及生长性能的影响

本文旨在研究日粮不同代谢能和粗蛋白质水平对合浦鹅日粮干物质、粗蛋白质、粗脂肪、粗纤维、钙和磷等养分的消化率及生长性能的影响.将120只28日龄的合浦鹅随机分成6个组,进行2个饲养和消化试验:试验1组饲喂粗蛋白质水平为15.10%,代谢能水平分别为10.03、11.00和11.85 MJ/kg的3种日粮;试验2组饲喂代谢能水平为11.50 MJ/kg,粗蛋白质水平分别为12.50%、14.85%和17.83%的3种日粮.用4 mol/L HCI ALA方法测定日粮的养分消化率.结果表明:粗蛋白质水平为15.10%和代谢能水平为10.03 MJ/kg的日粮,具有较高的养分消化率,但不同代谢能水平对试鹅的生长性能影响差异不显著(P>0.05);代谢能水平为11.50 MJ/kg和粗蛋白质水平为12.50%的日粮也具有较高的养分消化率,但不同粗蛋白质水平对试鹅的生长性能影响差异也不显著(P>0.05).因此,28日龄以后的合浦鹅的日粮粗蛋白质和代谢能水平分别为15.10%和10.03 MJ/kg,或分别为12.50%和11.50 MJ/kg时,均具有较高的养分消化率,但对合浦鹅的生长性能无显著影响.     

日粮代谢能水平对北京油鸡脂肪沉积和LPL基因表达的影响

试验旨在研究不同日粮代谢能水平对北京油鸡脂肪沉积及脂蛋白酯酶（LPL）基因mRNA表达影响。试验以3周龄北京油鸡公鸡为研究对象,将480只公鸡随机分为4组,分别饲喂4个代谢能水平的日粮（12.122、12.540、12.958和13.376 MJ/kg）,每个处理组5个重复,每个重复24只鸡。试验期为10周。采用荧光定量PCR方法检测LPL基因mRNA表达水平。结果表明,日粮代谢能水平对鸡体脂沉积各项指标和LPL基因mRNA表达水平影响极显著,随日粮代谢能水平提高,各项体脂沉积指标极显著上升,胸肌和腿肌IMF、皮脂重和皮脂率在ME3达到峰值,LPL基因mRNA表达水平在ME2组达到峰值（P＜0.01）。LPL基因mRNA表达水平与腹脂率、皮脂重和皮脂率呈显著正相关（P＜0.05）。研究结果提示,日粮代谢能水平显著影响北京油鸡体脂沉积和LPL基因mRNA表达;LPL基因表达影响北京油鸡体脂沉积状况。