共查询到19条相似文献,搜索用时 171 毫秒
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对国内大豆异黄酮研究论文中"术语混乱、含量及研究方法错误"等方面的探讨 总被引:4,自引:1,他引:3
大豆异黄酮是大豆生长过程中形成的一类次生代谢产物,包含染料木苷(Genistin)、染料木黄酮(Genistein)、黄豆苷(Daidzin)和黄豆苷元(Daidzein)等12种成分[1-3].从20世纪80年代起,很多研究报道以及动物实验表明,大豆异黄酮具有弱雌激素活性、抗氧化活性、抗溶血活性和抗真菌活性,能有效地预防和抑制白血病、骨质疏松、胃癌、乳腺癌和前列腺癌等多种疾病的发生,尤其是对乳腺癌和前列腺癌有积极的预防和治疗作用[4-6]. 相似文献
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外源茉莉酸对大豆异黄酮的影响 总被引:1,自引:0,他引:1
摘下生长8天的大豆子叶,在不同浓度茉莉酸溶液中浸泡一定时间,用甲醇提取大豆异黄酮,经高压液相色谱(HPLC)测定,研究子叶表面伤口、浸泡时间以及茉莉酸浓度对大豆异黄酮的影响.结果显示:在对照组(0.1%乙醇),子叶表面的伤口不影响异黄酮的分配比例;而用茉莉酸浸泡组,健康子叶中几乎不存在游离型的大豆甙元和染料木黄酮;丙二酰大豆甙和丙二酰染料木甙所占比例随着浸泡时间的延长而下降,大豆甙元和染料木黄酮所占比例随浸泡时间的延长而增加,大豆甙和染料木甙所占比例不随浸泡时间而变化;20μM茉莉酸溶液浸泡的子叶中异黄酮总量最低. 相似文献
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紫外分光光度法测定大豆总异黄酮的含量 总被引:8,自引:2,他引:8
建立了测定大豆提取物及以大豆展品黄酮为原料的保健食品中大豆总异黄酮的紫外分光光度法.以染料木素为标准品,优选出325nm为测量波长,以染料木素计量试样中的大豆总异黄酮.大豆提取物中大豆总异黄酮的含量为38.1%,加标回收率为102.3%;某保健品中大豆总总异黄酮的含量为4.76%,加标回收率为99.8%.方法简便、准确、重现性好,适用于保健品的日常分析和质量控制. 相似文献
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为促进苷元型异黄酮的开发和利用,采用光谱学手段研究大豆素/染料木素与大豆分离蛋白之间的作用机制.荧光和紫外光谱表明,大豆素/染料木素会使大豆分离蛋白色氨酸残基的微环境疏水性增强,造成蛋白质结构改变.大豆素/染料木素对大豆分离蛋白的猝灭机制为静态猝灭,主要作用力为范德华力和氢键,结合位点数为1;红外光谱表明,大豆素/染料木素的加入导致大豆分离蛋白的二级结构发生改变,随大豆素/染料木素质量浓度的增加,具体表现为,a-螺旋含量增多、β-折叠含量减少、β-转角含量增多,无规则卷曲含量增多.综合结果表明,染料木素与大豆分离蛋白的亲和力强于大豆素. 相似文献
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《中国油料作物学报》2017,(4)
为明确外源异黄酮类物质对大豆胞囊线虫的毒性,选取黄豆苷元(daidzein)、黄豆苷(daidzin)、染料木素(genistein)、染料木苷(genistin)、黄豆黄苷(glycitin)和黄豆黄素(glycitein)6种大豆异黄酮,采用贝氏皿法离体测定其对大豆胞囊线虫的卵孵化抑制率和对二龄幼虫(J2)的致死率及其田间防效,以明确对线虫有效的异黄酮种类。结果表明:6种大豆异黄酮对胞囊线虫的卵孵化和J2活性均具有一定的抑制作用,其中100μg/m L染料木素效果最好,处理24h后,对J2校正死亡率可达到45.46%,对卵孵化抑制率为43.48%,且50μg/m L染料木素处理种子后的田间防效显示对胞囊抑制率亦达到49.47%。此外,染料木苷和黄豆苷元对J2和卵孵化也有较明显效果但田间防效较其他异黄酮差;黄豆黄苷和黄豆黄素对J2毒杀作用较弱但对卵孵化抑制作用较强;黄豆苷对J2和卵孵化作用效果最弱,但是其田间防效与染料木素无显著性差异。说明供试的6种异黄酮在不同浓度和条件下均对大豆胞囊线虫有抑制作用,以染料木素的综合效果最好。研究结果为解释大豆抗线虫侵染的机制及利用大豆异黄酮防治线虫病害奠定了理论基础。 相似文献
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RP-HPLC测定大豆异黄酮缓释微丸胶囊的含量 总被引:1,自引:0,他引:1
为有效控制大豆异黄酮缓释微丸胶囊的质量,建立其中一种有效成分金雀异黄素含量的测定方法.采用RP-HPLC法测定大豆异黄酮缓释微丸胶囊中金雀异黄素的含量,固定柱:ZOBAX SB-C18(5μm,250 mm×4.6mm);流动相:乙腈-0.1%H3PO4水溶液(40:60);检测波长:262 nm;柱温:35℃;流速:1.0 mL·min-1;进样量5μL,应用外标一点法以峰面积定量.金雀异黄素在20.88~208.8 ng之间线性关系良好,回归方程为Y=4.38X-2.69,R=0.999(n=6),平均回收率为99.97%,RSD为0.87%(n=9).此法操作简便,结果准确可靠,精密度好,可用于大豆异黄酮缓释微丸胶囊中金雀异黄素的含量测定. 相似文献
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《中国油料作物学报(英文)》2020,5(1):1-5
The present study preliminarily evaluated neurotoxicity injuries induced by chlorpyrifos in PC12 cell, which were used as a model for nervous cell system. In cultured PC12 cell, application of soy isoflavones (genistein, daidzein, monomer and mixture) significantly reduced chlorpyrifos induced toxicity, a widely used pesticide, and resulted in a better cell survival rate. Treatments with isoflavones reduced malondialdehyde content, reactive oxygengeneration and acetylcholine level in medium, and maintained mitochondrial membrane potential integrity. Daidzein enhanced endogenous antioxidant system in PC12 cell with an increasing in superoxide dismutase per-unit activity. Genistein reduced acetylcholine content in the medium. Daidzein and genistein showed different effects, and their combined effect were greater than individual. In conclusion, soy isoflavones as an antioxidant and neuroprotectant, enhanced choline metabolism, which effectively mitigated disadvantageous influence of PC12 cell caused by chlorpyrifos. 相似文献
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通过比较在传统的淡豆豉(黑豆、黄豆)发酵工艺中加入人参后大豆异黄酮的含量变化,考察人参在淡豆豉发酵过程中对大豆异黄酮含量的影响。以大豆苷、染料木苷和染料木素为测定指标,采用高效液相色谱法进行含量测定。色谱柱为Agilent C18(150 mm×4.6 mm,5μm);流动相为甲醇-水-冰醋酸(40∶60∶0.5);流速0.5 mL.min-1;检测波长260 nm。结果以黄豆为原料发酵的淡豆豉,加入人参后大豆苷和染料木素的含量显著升高,染料木苷显著降低;以黑豆为原料发酵的淡豆豉,加入人参后大豆苷的含量显著降低,染料木苷和染料木素的含量显著升高。因此,淡豆豉传统发酵工艺中加入人参后,大豆异黄酮含量变化因原料不同而异。 相似文献
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以慢生型大豆根瘤菌(Bradyrhizobium japonicum)2-39为供试菌株,分别以不同浓度(5,10,15,20,25μg·mL~(-1))的大豆黄素、4-甲基伞形酮、金雀异黄酮、芒柄黄花素为诱导物,利用毛细管培养法对趋化的根瘤菌进行定量测定,研究不同种类和不同浓度的外源类黄酮对大豆根瘤菌趋化性的影响。选取对根瘤菌趋化性影响显著的外源类黄酮与大豆嫩丰16进行盆栽试验,进一步探究外源类黄酮对大豆结瘤效果的影响。结果表明:4种外源性类黄酮中,大豆黄素和金雀异黄酮对根瘤菌的趋化作用显著强于其它试验组,其最适作用浓度为15μg·mL~(-1)。盆栽试验结果显示:外源类黄酮大豆黄素和金雀异黄酮同时加入能够有效促进大豆结瘤。 相似文献
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Isoflavone Contents in Germinated Soybean Seeds 总被引:5,自引:1,他引:4
Zhu D Hettiarachchy NS Horax R Chen P 《Plant foods for human nutrition (Dordrecht, Netherlands)》2005,60(3):147-151
The effect of germination on isoflavone contents in two soybean varieties (Hutcheson and Caviness) was investigated. Soybean
seeds were soaked at 25 ∘C for 12 h, germinated at 40 ∘C, and freeze-dried. The isoflavone contents of dry, soaked, germinated (hypocotyl length at 0.5, 2.5, and 6.5 mm), and nongerminated
seeds were determined by high performance liquid chromatography. The maximum amount of total isoflavone, genistein, and daidzein
with their β-glucoside conjugates was obtained when hypocotyl length of the germinated-seed from var. Hutcheson was 0.5 mm
(2.491, 1.500, and 0.671 mg/g), and from var. Caviness was 2.5 mm (2.78, 1.523, and 0.905 mg/g). A dramatic increase in malonylgenistin
and malonyldaidzin (1.305 mg/g and 0.476 mg/g in Hutcheson, and 1.308 mg/g and 0.677 mg/g in Caviness, respectively) was observed
at these hypocotyl lengths. A decrease was observed after this stage. Genistein and daidzein contents were highest just after
soaking. Glycitein and its β-glucoside conjugates remained almost the same during germination. Controlled germination can
be used to enhance isoflavone content in soybean seed. 相似文献
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建立淡豆豉中多指标成分含量测定方法,采用HPLC法同时测定淡豆豉中大豆苷元和染料木素含量,UV法测定总异黄酮含量。色谱条件:Agilent HC-C18色谱柱(250×4.6 mm,5.0μm);甲醇-0.1%冰醋酸(50∶50)为流动相;流速1 mL.min-1;检测波长261 nm;柱温30℃;进样量10μL。紫外分光光度计采用261 nm波长测定淡豆豉提取液的吸光度值。结果表明:大豆苷元和染料木素色谱峰的分离度良好,淡豆豉中大豆苷元、染料木素的保留时间分别为9.79和14.87 min,峰面积积分值与进样量呈良好的线性(r>0.9999),平均回收率分别为98.90%和100.53%。总异黄酮含量测定线性范围为1.44~7.20μg.mL-1,平均回收率为102.26%。该法简便易行,能够用于评价淡豆豉质量。 相似文献
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Dong-Yang Zhang Yuan-Gang Zu Yu-Jie Fu Meng Luo Wei Wang Cheng-Bo Gu Chun-Jian Zhao Jiao Jiao Thomas Efferth 《Industrial Crops and Products》2012,37(1):311-320
An enzyme pretreatment and negative-pressure cavitation extraction (E-NPCE) method was investigated for extraction genistein and apigenin from pigeon pea roots. The important parameters involved in E-NPCE process were optimized by single-factor experiments and then critical parameters were investigated by a 23 full factorial central composite design (CCD) to optimize extract conditions. Under optimal conditions, the yields of genistein and apigenin were 0.628 mg/g and 0.359 mg/g, which represented an increase of 44.70% and 53.05%, respectively, compared to standard NPCE. Furthermore, from DPPH scavenging activity test the extract of E-NPCE showed better antioxidant activity than these of other methods. The results demonstrated that E-NPCE would have lower energy consumption, higher efficiency and could be an alternative method for natural compounds extraction. 相似文献