猪旋毛虫检验方法探析

随着人民生活水平的提高,人们对肉品卫生安全甚为关注。猪的旋毛虫病是一种人畜共患寄生虫病,旋毛虫的宿主是猪和犬,旋毛虫病猪肉对人的危害十分严重,现介绍几种检验方法,供参考。1旋毛虫镜检法旋毛虫镜检法,具体操作方法是:从膈肌或其它规定部位取4～5克肉样,每一肉样剪取12块如麦粒大小的肉,压在两玻片之间,用旋毛虫镜检器或显微镜观察。2旋毛虫的“目检法”“目检法”即用眼睛观察肉样来检验旋毛虫。旋毛虫幼虫感染猪体后,大约21天至7个月形成钙化,肉眼观察即能检出。具体操作法是:取新鲜横膈膜肌脚,撕去肌膜,肌肉纵向拉平,然后在光线较…     

猪旋毛虫检验方法探析

随着人民生活水平的提高，人们对肉品卫生安全和身体健康甚为关注。猪的旋毛虫病是一种人畜共患寄生虫病，严重危害人畜健康。旋毛虫病的宿主是猪和犬，旋毛虫猪肉是人旋毛虫的重要传染来源。旋毛虫猪肉快速检验是防止旋毛虫病既经济又可靠的办法，现介绍几种检验方法，供参考。     

旋毛虫病猪肉的目检法及其应用

平顶山市郊县养猪业十分兴旺，城市市民肉食食品主要以猪肉为主，市区的动物检疫工作始于一九八四年。多年来，我们一直从事动物检疫和肉品质量检验工作，为让市民吃上放心肉，现就旋毛虫病猪肉的［］检法谈一点体会。实际工作中，诸旋毛虫病的检疫多采用消化法和压片法，却忽视了自检法，甚至有人认为，只有前述两种方法效果较好，肉眼根本发现不f旋毛虫虫体。其实，目检法方便易行，操作简单，如结合压片法，可以大大提高准确率。现将目检法的检验程序简介如下，供同行们参考。旋毛虫病猪肉国检法：即用眼睛直接观察肉样是否有旋毛虫（病…     

浅析猪旋毛虫病的发生与防制

猪旋毛虫病是由旋毛虫引起的一种人畜共患寄生虫病,成虫寄生于肠管,幼虫寄生于横纹肌。人、猪、犬、猫、鼠类、狐狸、狼、野猪等均能感染,鸟类可以试验感染。人若摄食了生的或未煮熟的含旋毛虫包囊的猪肉可患病致死,故肉品卫生检验中将旋毛虫列为首要项目。     

快速检测猪旋毛虫病

旋毛虫病是由毛首目毛形科的旋毛形线虫引起的人畜共患寄生虫病,也是一种自然疫源性疾病。成虫寄生于肠管,幼虫寄生于横纹肌。人旋毛虫病可致人死亡,感染来源于摄食了生的或未煮熟的含旋毛虫包囊的猪肉,所以肉品卫生检验中将旋毛虫列为首要项目。旋毛虫病分布于世界各地,宿主包括人、猪、狗、鼠、猫等100余种动物,甚至不吃肉的鲸也可以感染旋毛虫病。人感染旋毛虫病多与吃生猪肉,或食用腌制与烧烤不当的猪肉制     

猪肉旋毛虫三种检查方法比较

猪肉旋毛虫三种检查方法比较旋毛虫病是一种人畜共患寄生虫病，在众多可以感染的动物中，猪是人的重要传染来源。在肉品卫生检验中不漏检旋毛虫病，对于公共卫生有重要意义。为此，我们应用压片法、消化法以及切片法进行检查，比较其检出率，并详细地观察了其显微镜病理变...     

(60)~Coγ射线辐照对旋毛虫影响的研究

旋毛虫病是由毛首目、毛形科的旋毛虫(Trichinella Spiralis)引起的人畜共患病。家畜中以猪的感染率最高。人患旋毛虫病主要是食入生的或未煮熟的旋毛虫病猪肉而感染。使生猪肉不具有旋毛虫传染性的一种有前途的方法就是γ辐照,美国已无条件批准γ辐照鲜猪肉,以控制旋毛虫病。~60Coγ射线辐照对旋毛虫影响的研究,其主要目     

猪旋毛虫病的有效诊治和预防

猪旋毛虫病是由旋毛虫成虫寄生于猪的小肠,幼虫寄生于横纹肌而引起的人畜共患病。此病是人畜重要的寄生虫病,人感染可引起死亡。有吃生猪肉或未煮熟猪肉的习惯,因而易发生本病。本病在公共卫生上极为重要,各地肉品要加强对本病的检验。     

胃蛋白酶消化法检验旋毛虫

旋毛虫属毛形科，是人畜共通寄生虫。人体感染旋毛虫后，可引起多种病症，严重的可导致死亡而人旋毛虫病的主要感染来源是摄食了生的或未熟透的含有旋毛虫包囊的猪肉或其他肉类，因此，旋毛虫的检验是肉品卫生检验的重要项目之一。旋毛虫检验一般采用压片镜检法，但由于这种方法需逐头猪检验，比较繁琐，而有些地方又不常见旋毛虫，如本市近两年来都未曾发现过旋毛虫，针对这些地方，可采用消化法检验旋毛虫以提高工作效率。消化法可一次性检验数拾到数百头猪，各地可根据需要灵活掌握。现将消化法简要介绍如下，供同行参考。 检验程序：采…     

快速检测猪旋毛虫病

旋毛虫病是由毛首目毛形科的旋毛形线虫引起的人畜共患寄生虫病,也是一种自然疫源性疾病。成虫寄生于肠管,幼虫寄生于横纹肌。人旋毛虫病可致人死亡,感染来源于摄入了生的或未煮熟的含旋毛虫包囊的猪肉,所以肉品卫生检验中将旋毛虫列为首要项目。     

癸氧喹酯干混悬剂含量测定的改进

采用高效液相色谱法测定癸氧喹酯干混悬剂的含量,在2-250μg/mL范围内,峰面积的常用对数与进样量浓度的常用对数呈良好的线性关系,R^2=1(n=5),平均回收率为99.24%~99.51%,RSD在0.05%~0.28%。此方法分析时间短,样品前处理简便、定量结果准确,重现性好,结果满意,为其质量控制提供了依据。     

商会自治的基石——商会自治规范研究

在现代法律秩序中,商会自治规范是制定法的基础和必要的补充,甚至在某些方面替代了制定法;商会自治规范主要包括商会组织规范、行为规范、惩罚规范以及争端解决规范等;其效力仅及于其内部成员;商会自治规范和制定法之间存在冲突,但也存在整合的基础。     

猪毛色遗传的研究进展

本文概述了猪的毛色类型、猪的毛色遗传模式,着重综述了猪毛色基因分子基础的研究进展,指出存在问题并就未来发展方向做了思考。     

中华人民共和国农业部公告 第267号

为贯彻落实《兽药生产质量管理规范》(简称《兽药GMP》),进一步推动兽药GMP实施进程,我部制定了《兽药生产质量管理规范检查验收办法》,现予公告。本公告自2003年6月1日起施行。附件:兽药生产质量管理规范检查验收办法二○○三年四月十日第一章 总则 第一条 为推动《兽药生产质量管理规范》(以下简称兽药GMP)的实施,规范兽药GMP检查验收工作,制定本办法。 第二条 农业部负责全国兽药GMP管理和检查验收工作;负责制修订兽药GMP检查验收管理规定;负责兽药GMP检查员队伍建设和监督管理工作,负责国际兽药贸易中GMP互认工作。 …     

鸡败血支原体垂直传播模式及其阻断方法

以国际标准强毒Ｒ株人工感染非免疫产蛋鸡,定时扑杀,分别从鼻窦、眶下孔、气管、肺、气囊、卵巢和输卵管分离ＭＧ,并收集感染鸡所产蛋分离ＭＧ。结果表明,人工感染４８小时后上、下呼吸道及肺已被全面感染,９６小时气囊已被感染,１２０小时输卵管已能分离到ＭＧ,卵巢始终分离不到ＭＧ。人工感染鸡自１４４小时便能在其所产蛋中分离出ＭＧ。药物治疗能在７２小时内消除感染,油乳剂苗则需２４天后逐渐降低蛋内ＭＧ分离率,药物卵内注射、种蛋药浴、高温处理均能杀死卵内ＭＧ,但以研制的种蛋浸泡剂药浴效果为最好。     

Comparison of 2 methods of centesis of the bursa of the biceps brachii tendon of horses

REASONS FOR PERFORMING STUDY: Centesis of the bicipital bursa using an 8.9 cm long spinal needle has been reported but the alternative of employing a 3.8 cm long hypodermic needle requires validation. OBJECTIVE: To compare the efficacy of 2 different methods of centesis of the bicipital bursa and to evaluate the usefulness of ultrasonographic imaging to determine the location of solution administered when centesis of the bursa is attempted. METHODS: For Trial 1, 6 clinicians, who had no previous experience of centesis of the bicipital bursa, attempted to inject a solution composed of an aqueous radiopaque contrast medium and physiological saline solution (PSS) into the bicipital bursae of 2/12 horses using the previously described distal approach to inject one bursa and a proximal approach to inject the contralateral bursa. The bicipital tendon and bursa were examined ultrasonographically before and after injection; and both shoulders were examined radiographically to identify the location of the medium. In Trial 2, another 6 clinicians, also with no previous experience of centesis, repeated Trial 1, using 6 horses, but the radiopaque contrast medium was mixed with air instead of PSS. RESULTS: Accuracy of centesis using the proximal approach was 39% and that of the distal approach 28%. Ultrasonographic examination of the shoulder allowed the location of solution and air to be accurately predicted in all 12 shoulders examined. CONCLUSIONS: Clinicians who have had no previous experience performing centesis of the bicipital bursa are unlikely to be successful in centesis using either approach. Radiographic examination after injecting a radiopaque contrast medium may be necessary to assess the success of centesis especially if bursal fluid is not obtained during centesis. Injecting air along with the radiopaque contrast medium provides more accurate ultrasonographic confirmation of centesis and better radiographic definition than does injection without air.     

不同样本商品蜂蜜中硒含量的测定

用硝酸和高氯酸消化蜂蜜,使硒游离出来,在微酸性环境下,硒和2,3-二氨基萘(DAN)生成有较强荧光的物质,用环己烷萃取,在激发波长378nm,荧光波长518nm处测定其荧光强度。蜂蜜中硒含量范围:0.10～0.82μg/g。表明:蜂蜜应视为天然富硒营养品。     

乳酸杆菌益生作用机制的研究进展

乳酸杆菌作为益生菌广泛用于人和动物。本文综述了乳酸杆菌改善宿主健康的机制。乳酸杆菌可通过产生抗菌物质如乳酸、过氧化氢、细菌素,或者通过竞争营养或肠道黏附位点来抑制致病菌;通过诱导黏附素的分泌或阻止细胞凋亡而增强肠道的屏障功能,从而保护肠道。文章重点讨论了乳酸杆菌表面成分（表面蛋白、脂磷壁酸和肽聚糖）与肠道受体（Ｃ型凝集素受体、Ｔｏｌｌ样受体和 Ｎｏｄ样受体）,阐述了他们结合后启动免疫调节信号,调控肠道免疫功能以发挥改善健康作用的机制。     

Effects of size of ingestively masticated fragments of plant tissues on kinetics of digestion of NDF

Ingestively masticated fragments were collected and sized via sieving. Different sizes of esophageal masticate and ruminal digesta fragments, and ground fragments of larger masticated pieces were incubated in vitro, and undigested NDF remaining at intervals of up to 168 h of incubation was determined. The ruminal age-dependent time delay (tau) for onset of digestion of NDF was positively correlated (P < 0.004) with the mean sieve aperture estimated to retain 50% of the fragments between successive sieve apertures (MRA). Degradation rate of potentially degradable NDF (PDF) and level of indigestible NDF were not related (P > 0.10) to MRA of masticated and ground fragments. Estimates of tau were positively related to MRA, with slopes of bermudagrass < corn silage < ruminal fragments of corn silage. It was concluded that fragment size-, and consequently, ruminal age-dependent onset of PDF degradation of a mixture of different fragment sizes results in an age-dependent rate of degradation of the more rapidly degrading of two subentities of PDF. Models are proposed that assume a tau before onset of simultaneous degradation of PDF from two pools characterized as having gamma-modeled age-dependency and age-constant rates. The ruminal age-dependent pool seems to be associated with the faster-degrading pool, and its rate parameter increases with range in MRA in the population of fragments. Conceptually, the ruminal age-dependent rate parameter for PDF degradation seems to represent a composite of several effects: 1) effects of the size-dependent tau; 2) range in MRA of the population of ingestively masticated fragments; and 3) subentities of PDF that degrade via more rapid age-dependent rates compared with subentities of PDF that degrade via age-constant rates. The estimated fractional rates of ruminative comminution of ingestively masticated fragments (0.060 to 0.075/h) were of a magnitude similar to the mean fractional rates of PDF digestion (0.030 to 0.085/h), which implies that ruminative comminution may be first-limiting to fractional rate of PDF digestion. The in vivo roles of ingestive and ruminative mastication of fragments on PDF degradation must be considered in any kinetic system for estimating PDF digestion in the rumen. These results and others in the literature suggest that the rate of surface area exposure rather than intrinsic chemical attributes of PDF may be first-limiting to degradation rate of PDF in vivo.