| 高效液相色谱法测定微球中硫酸小檗碱的含量 |
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| 引用本文： | 朱庆贺,王丽坤,张艳,王 爽,陈 曦,王观悦,穆永才,罗天瑶,史同瑞.高效液相色谱法测定微球中硫酸小檗碱的含量[J].中国兽药杂志,2020,54(2):34-38. |
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| 作者姓名： | 朱庆贺 王丽坤 张艳 王 爽 陈 曦 王观悦 穆永才 罗天瑶 史同瑞 |
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| 作者单位： | 黑龙江省农业科学院畜牧兽医分院,,,,,,,, |
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| 基金项目： | 黑龙江省应用技术研究与开发项(czznkt2018-2) |
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| 摘 要： | 建立了测定微球中硫酸小檗碱含量的高效液相色谱法(HPLC)。采用C18色谱柱(4.6×250 mm,5μm),以乙腈-水(45∶55)为流动相,流速1.0 mL/min,检测波长349 nm,进样量5μL,柱温30℃。硫酸小檗碱浓度在0.098~49.00μg/mL时,其峰面积与浓度线性关系良好(R 2=0.9991),无杂质干扰,稳定性高,平均回收率为98.98%。本操作方法简便,分析快速,结果准确,适用于微球中硫酸小檗碱的含量测定。
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| 关 键 词： | 硫酸小檗碱 微球 高效液相色谱法 含量 |
| 收稿时间： | 2019/11/6 0:00:00 |
| 修稿时间： | 2020/1/14 0:00:00 |
Content Determination of Berberine sulfuric Microspheres by HPLC |
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| ZHU Qing-he,WANG Li-kun,ZHANG Yan,YANG Xu-dong,WANG Shuang,CHEN Xi,WANG Guan-yue,MU Yong-cai,LUO Tian-yao,SHI Tong-rui.Content Determination of Berberine sulfuric Microspheres by HPLC[J].Chinese Journal of Veterinary Drug,2020,54(2):34-38. |
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| Authors: | ZHU Qing-he WANG Li-kun ZHANG Yan YANG Xu-dong WANG Shuang CHEN Xi WANG Guan-yue MU Yong-cai LUO Tian-yao SHI Tong-rui |
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| Institution: | Branch of Animal Husbandry and Veterinary of Heilongjiang Academy of Agricultural Sciences,Heilongjiang,,,,,,,, |
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| Abstract: | A method for the content determination of berberine sulfuric in microspheres by HPLC was established.HPLC system consisted of C18 column(5μm 4.6×250 mm),methyl alcohol-water(45∶55)as mobile phase.The flow rate was 1.0 mL/min.The detection wave length was 349 nm.The injection volume was 5μL and column temperature was 30℃.There was no interference occurred from inpurities.The calibration curve was linear in the range of 0.098~49.00μg/mL(R 2=0.9991).The average recovery was 98.98%.The method is rapid,simple and accurate for the content determination of berberine sulfuric suspo-emulsions. |
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| Keywords: | Berberine sulfuric Microspheres HPLC Content |
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