| 表达马立克氏病病毒CVI988／Rispens株糖蛋白B基因的重组鸡痘病毒疫苗的免疫保护研究 |
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| 引用本文： | 彭大新,刘秀梵,吴长新,吴艳涛,文其乙,高崧,张如宽,吴晓丰,邢力,朱爱华,詹爱军.表达马立克氏病病毒CVI988／Rispens株糖蛋白B基因的重组鸡痘病毒疫苗的免疫保护研究[J].畜牧兽医学报,2001,32(2):139-145. |
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| 作者姓名： | 彭大新 刘秀梵 吴长新 吴艳涛 文其乙 高崧 张如宽 吴晓丰 邢力 朱爱华 詹爱军 |
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| 作者单位： | 扬州大学畜牧兽医学院, |
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| 基金项目： | 本研究由国家863计划，（960332025）；国家自然科学基金（39670558）；江苏省科委自然科学基金资助（BK97069）。 |
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| 摘 要： | 用鸡痘病毒载体表达马立克氏病病毒（MDV）糖蛋白B（gB）基因构建成重组鸡痘病毒（rFPV）。以MDV GA或Md5和RBIB混合攻毒，在不同品种的鸡中评价rFPV－gB／R单价苗和与火鸡疱疹病毒（HVT）组成的二价苗的免疫保护效力。试验结果表明，MDV疫苗和FPV母源抗体阴性的SPF鸡和母源抗体阳性的商品鸡中，rFDV－gB／R均能提供免疫保护作用，且其中一种商品蛋鸡中rFPV－gB／R与HVT液氮苗或HVT冻干苗结合使用，均有显著的免疫协同保护作用。免疫学研究指出，rFPV－gB／R与常规疫苗一样，可显著地降低疫苗免疫攻毒鸡的病毒血症和羽囊排毒。
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| 关 键 词： | 重组鸡痘病毒 糖蛋白B基因 马立克氏病毒 CVI988／Rispens株 保护研究 |
| 文章编号： | 0366－6964（2001）02－0139－07 |
| 修稿时间： | 1999年9月23日 |
PROTECTION STUDIES OF A RECOMBINANT FOWLPOX VIRUS VACCINE EXPRESSING GLYCOPROTEIN B GENE FROM CVI988／RISPENS STRAIN OF MAREK’S DISEASE VIRUS |
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| Peng Daxin,Liu Xiufan,Wu Changxin,Wu Yantao,Wen Qiyi,Gao Song,Zhang Rukuan,Wu Xiaofeng,Xing Li,Zhu Aihua,Zhan Aijun.PROTECTION STUDIES OF A RECOMBINANT FOWLPOX VIRUS VACCINE EXPRESSING GLYCOPROTEIN B GENE FROM CVI988／RISPENS STRAIN OF MAREK’S DISEASE VIRUS[J].Acta Veterinaria et Zootechnica Sinica,2001,32(2):139-145. |
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| Authors: | Peng Daxin Liu Xiufan Wu Changxin Wu Yantao Wen Qiyi Gao Song Zhang Rukuan Wu Xiaofeng Xing Li Zhu Aihua Zhan Aijun |
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| Abstract: | Recombinant fowlpox Virus （rFPV） was constructed to express glycoprotein B （gB） gene from CVI988／Rispens strain of Marek’s disease virus （MDV）．The rFPV－gB／R alone and in combination with herpesvirus of turkey （HVT） preparations were evaluated for their protective efficacy against challenge with different MDV strains in different chickens．The rFPV－gB／R alone induced protection comparable to that by HVT vaccines in both Ab-SPF chickens and Ab＋ production chickens．Significant protective synergism was observed in one of the two commercial production chickens when rFPV－gB／R was combined with HVT of either cell associated or cell free preparations．Immunogenesis studies showed that rFPV－gB／R，just like conventional vaccines，significantly reduced the level of viremia and feather follicle shedding with virus in vaccinated chickens． |
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