海洋侧孢短芽孢杆菌S-12-86溶菌酶的分离纯化与冻干技术研究 |
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作者姓名: | 纪存朋 孙谧 于建生 郑媛 |
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作者单位: | 1. 中国水产科学研究院黄海水产研究所,青岛,266071;青岛科技大学化工学院,266042 2. 中国水产科学研究院黄海水产研究所,青岛,266071 3. 青岛科技大学化工学院,266042 |
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基金项目: | 国家863计划项目(2006AA10Z349和2007AA091602)资助 |
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摘 要: | 海洋微生物溶菌酶发酵液经低温离心、超滤浓缩、乙醇提取、Superdex 75 10/300凝胶层析和反相高效液相色谱层析纯化得到电泳纯海洋溶菌酶,分子量为17.1 kD,比活达到3 987.7 U/mg,纯度提高41.98倍,活力回收率为21.7%。对该酶冷冻干燥过程的研究结果表明,海藻糖、蔗糖和麦芽糖对该酶均有一定的冻干保护作用。其中,以海藻糖的保护效果最佳。0.5 mol/L海藻糖与20 mg/ml吐温80复合作为冻干保护剂,使冻干后的溶菌酶活性维持在95%以上,为该酶进一步研究和应用提供了稳定的酶制剂。
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关 键 词: | 海洋微生物 溶菌酶 分离纯化 冷冻干燥 |
收稿时间: | 2009/3/5 0:00:00 |
修稿时间: | 2009/4/30 0:00:00 |
Purification and lyophillization of Lysozyme produced by marine microorganism |
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Authors: | JI Cun-peng SUN Mi YU Jian-sheng ZHENG Yuan |
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Institution: | 1 Yellow Sea Fisheries Research Institute;Chinese Academy of Fishery Sciences;Qingdao 266071;2 College of Chemical Engineering;Qingdao University of Science & Technology;266042 |
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Abstract: | Cell-free supernatant with marine microorganism lysozyme was prepared by centrifugation of culture broth,ultrafiltration and concentration.The crude lysozyme was purified 41.98 fold to electrophoretic homogeneity with recovery of 21.7% and specific activity of 3 987.7 U/mg by extracting with ethanol,Superdex 75 10/300 chromatography and reversed-phase HPLC.The relative molecular weight of this lysozyme was 17.1 kD determined by SDS-PAGE electrophoresis.Preparations of lysozyme from native marine microorgani... |
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Keywords: | Marine microorganism Lysozyme Purification Lyophillization |
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