一份水稻矮杆突变体的形态特征和基因定位(英文)

目的]对一株水稻矮杆突变体的形态特征进行分析,同时进行基因定位。方法]以一株矮秆突变体(潇湘矮)为研究对象进行形态观察,并以潇湘矮作母本,半矮杆材料湘早143为父本,构建群体进行遗传分析,同时利用微卫星标记进行基因定位。结果]潇湘矮的平均株高为55cm;遗传分析结果显示所有F1株高偏向于父本,F2群体出现半矮杆植株和矮杆植株,比例接近于3:1性状分离,证实潇湘矮株高性状受1对主效隐性基因控制;将该基因定位于水稻第5染色体上,位于RM249上游,遗传距离为8.4cM。结论]rRH是一个新的水稻矮杆基因。

Morphological Analysis and Gene Mapping of a Rice Dwarf Mutant

Objective] This study aimed to conduct morphological analysis and gene mapping of a rice dwarf mutant.Method] A dwarf mutant(Xiaoxiang’ai) was used as test material for morphological observation.Xiaoxiang’ai was used as female parent and semi-dwarf material Xiangzao143 was used as male parent to construct populations for genetic analysis.Gene mapping was conducted by using micro-satellite markers.Result] The average height of Xiaoxiang’ai was 55 cm;genetic analysis results showed that plant height of F1 individuals was similar with the male parent,while semi-dwarf plants and dwarf plants were observed in F2 populations and the segregation ratio was nearly 3:1,indicating that plant height trait of Xiaoxiang’ai is mainly controlled by one pair of recessive gene located on rice chromosome 5 and in the upstream of RM249,with a genetic distance of 8.4 cM.Conclusion] rRH is a new dwarf gene in rice.