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| 番鸭随机扩增多态性DNA及遗传多样性研究 | |
| 引用本文： | 苏瑛,刘楚吾,叶昌辉,刘丽,黄毓青,张锦,蔡笛扬.番鸭随机扩增多态性DNA及遗传多样性研究[J].甘肃农业大学学报,2006,41(3):16-21. |
| 作者姓名： | 苏瑛 刘楚吾 叶昌辉 刘丽 黄毓青 张锦 蔡笛扬 |
| 作者单位： | 1. 甘肃农业大学,甘肃,兰州,730070;广东海洋大学,广东,湛江,524088 2. 广东海洋大学,广东,湛江,524088 3. 湛江坡头区畜牧局,广东,湛江,524047 |
| 基金项目： | 广东省资助项目 , 广东省湛江市科技攻关项目 |
| 摘 要： | 利用随机扩增多态DNA(RAPD)技术分析番鸭的遗传多样性,20个引物共扩增出164条带,分子量约在200 ～2 000 bp之间,番鸭20个个体间遗传距离为0．4572～0．0177,群体内个体间遗传相似度(F)为0．9823～0．5428,遗传多样性指效(Ho)平均为0．1639．结果表明番鸭DNA的同源性高,品种的纯度保持较好． |
| 关 键 词： | 番鸭 RAPD 遗传结构 遗传多样性 |
| 文章编号： | 1003-4315（2006）03-0016-06 |
| 收稿时间： | 2005-06-13 |
| 修稿时间： | 2005-06-13 |
Random amplified polymorphic DNA(RAPD) and genetic diversity of muscovy |
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| SU Ying,LIU Chu-wu,YE Chang-hui,LIU Li,HUANG Yu-qing,ZHANG Jin,CAI Di-yang.Random amplified polymorphic DNA(RAPD) and genetic diversity of muscovy[J].Journal of Gansu Agricultural University,2006,41(3):16-21. | |
| Authors: | SU Ying LIU Chu-wu YE Chang-hui LIU Li HUANG Yu-qing ZHANG Jin CAI Di-yang |
| Institution: | 1. Gansu Agrieultural University, Lanzhou 730070; 2. Guangdong Oeean University, Zhanjiang 524088 3. Zhanjiang Potou Bureau of Animal Husbandry,Zhanjiang 524047,China |
| Abstract: | Random amplified polymorphic DNA (RAPD) was used to estimate the genetic diversity of muscovy,twenty 10 bp single primers sequence were used to amplify genomic DNA. 164 bands of 200-2 000 bp were got. The results showed that the genetic distance(D)was 0.4572-0.0177,the genetic similarity among individuals(F)was 0.9823-0.5428 and average index of genetic diversity(H。)of 20 musco-vies was 0.1639. This indicated that muscovies DNA had high homology, and the breed pure was kept well. |
| Keywords: | muscoy RAPD genetic structure genetic diversity |
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