对硫磷对皱纹盘鲍血细胞吞噬化学发光的影响

在体外条件下,利用化学发光法定量研究了对硫磷浓度对皱纹盘鲍Haliotis discus Hannm血细胞吞噬化学发光的影响。结果表明,用对硫磷浸泡1h和12h处理的皱纹盘鲍血细胞都能够抑制鲍血细胞吞噬时的化学发光,即抑制了血细胞吞噬时释放活性氧(BOS)杀菌的活性,而且这种抑制程度随着对硫磷浓度的增加而加强。用对硫磷浸泡处理皱纹盘鲍血细胞后,测定了血细胞吞噬酵母细胞的吞噬百分率和吞噬指数,结果显示,用低浓度的对硫磷(0．5、5．0、10．0mg／L)处理的血细胞能提高其吞噬酵母细胞的活力;用高浓度的对硫磷(20、30、40mg／L)处理的血细胞能抑制其吞噬酵母细胞的活力。研究还表明,当水中对硫磷浓度为20mg／L以上时,对皱纹盘鲍血细胞免疫活性有抑制作用。

Effects of parathion on the chemiluminescent response of Haliotis discus Hannai hemocytes in the phagocytosis

Reactive oxygen species(ROS)-dependent bacterial killing mechanisms of Haliotis discus Hannai hemocytes were quantified in vitro by luminol dependent chemiluminescence (CL) assay to determine whether the mechanisms were affected by organophosphorous pesticide, parathion. In vitro immersion of the hemocytes in parathion for 1 hr or 12 hr inhibited ROS production and resulted in dose-dependent suppression of CL. The phagocytic index and phagocytic percentage of the hemocytes immersed in parathion to phagocytize yeast cells were assayed. The results indicated that the lower-concentration of parathion (0.5, 5,10 mg/L) enhanced the activities of Haliotis discus Hannai hemocytes to phagocytize yeast cells, while the high-concentration of parathion (20, 30, 40 mg/L) inhibited the activities of Haliotis discus Hannai hemocytes to phagocytize yeast cells.