HPLC同时测定栀子金花丸中4种成分的含量

目的]采用HPLC建立同时测定栀子金花丸中黄芩苷、汉黄芩苷、黄芩素、汉黄芩素这4种成分含量的分析方法。方法]色谱柱为Agilent Zorbax SB-C18(4.6 mm×250 mm,5μm);流动相为乙腈-浓度0.1%磷酸溶液,梯度洗脱,检测波长为278 nm。结果]黄芩苷、汉黄芩苷、黄芩素、汉黄芩素4种成分的线性范围分别为0.418~4.18μg/ml(R=0.999 8),0.2~2.0μg/ml(R=0.999 8),0.233~2.330μg/ml(R=0.999 9),0.215~2.150μg/ml(R=0.999 9);平均回收率分别是98.40%(RSD=0.45%)、98..66%(RSD=1.41%)、98.88%(RSD=1.48%)和98.97%(RSD=1.45%)。结论]该方法快速、简便,且具有良好的重复性和回收率,可作为栀子金花丸中这4种成分的含量控制方法。

Simultaneous Determination of 4 Components in Zhizijinhua Pill by HPLC

Objective] To establish a method for the simultaneous determination of Baicalin,Wogonoside,Baicalein and Wogonin in Zhizijinhua Pill using HPLC.Method] The separation was carried out on a Agilent Zorbax SB-C_（18）（4.6 mm × 250 mm,5μm）.The mobile phase was methanol-0.1％ phosphoric acid with gradient elution and 278nm wavelength detection.Result] The Baicalin,Wogonoside,Baicalein,Wogonin had good linearity in the ranges of 0.418-4.18 μg/ml （R =0.999 8）,0.2-2 μg/ml （R =0.999 8）,0.233-2.330μg/ml（R =0.999 9） and 0.215-2.150 μg/ml（R =0.999 9）.The average recoveries were 98.40％ （RSD =0.45％）,98.66％ （RSD =1.41％）,98.88 ％ （RSD =1.48 ％）,98.97 ％ （RSD =1.45 ％）,respectively.Conclusion] This method is rapid,simple with good repeatability and recovery,so it can be used as a quantitative analysis method for Zhizijinhua Pill.