HPLC同时测定栀子金花丸中4种成分的含量

[目的]采用HPLC建立同时测定栀子金花丸中黄芩苷、汉黄芩苷、黄芩素、汉黄芩素这4种成分含量的分析方法。[方法]色谱柱为Agilent Zorbax SB-C18(4.6 mm×250 mm,5μm);流动相为乙腈-浓度0.1%磷酸溶液,梯度洗脱,检测波长为278 nm。[结果]黄芩苷、汉黄芩苷、黄芩素、汉黄芩素4种成分的线性范围分别为0.418~4.18μg/ml(R=0.999 8),0.2~2.0μg/ml(R=0.999 8),0.233~2.330μg/ml(R=0.999 9),0.215~2.150μg/ml(R=0.999 9);平均回收率分别是98.40%(RSD=0.45%)、98..66%(RSD=1.41%)、98.88%(RSD=1.48%)和98.97%(RSD=1.45%)。[结论]该方法快速、简便,且具有良好的重复性和回收率,可作为栀子金花丸中这4种成分的含量控制方法。     

Effect of wogonin on OSCC cell growth and invasion

AIM: To investigate the molecular mechanism of wogonin on the growth and invasion of oral squamous-cell carcinoma (OSCC) cell line SCC-4. METHODS: After treatment with various doses (0, 20, 40, 60, 80 and 100 mg/L) of wogonin for the indicated time, MTT assay was used to evaluate cell viability. The cell apoptosis was detected by flow cytometry with Annexin V and propidium iodide (Annexin V/PI) double staining. The cell invasion ability was analyzed by Transwell assay. The activation of Wnt/β-catenin signaling molecules was assessed by Western blot. RESULTS: Wogonin inhibited the viability and invasion of SCC-4 cells but promoted cell apoptosis in a dose-and time-dependent manner. Wogonin treatment obviously decreased the activation of β-catenin. Moreover, the expression of downstream targets cyclin D1, matrix metalloproteinase-2 (MMP-2) and MMP-9 were obviously down-regulated, accompanied by the up-regulation of anti-apoptotic protein Bcl-2. Wnt/β-catenin activator LiCl remarkably attenuated the inhibitory effect of wogonin on the activation of Wnt/β-catenin signaling molecules. Importantly, the inhibition of cell growth and invasion ability by wogonin treatment was dramatically attenuated after LiCl exposure. CONCLUSION: Wogonin blocks SCC-4 cell growth and invasion mainly by regulating Wnt/β-catenin signaling, indicating that it is a potential suppressor for OSCC and may be a potential target for the development of anti-OSCC therapy.     

纤维素酶辅助提取生黄芩饮片中黄酮苷元的工艺研究

[目的]探讨生黄芩饮片黄酮类化合物的纤维素酶辅助提取优化工艺条件。[方法]采用系列单因素和正交试验设计,通过高效液相分析以黄酮苷转化成的苷元含量为考核指标,对影响辅助提取的因素进行了研究,并与传统水煎煮法比较。[结果]生黄芩黄酮苷元的最佳纤维素酶辅助提取工艺为液固比10：1,酶浓度25U／g（药材）,pH值5．O,温度50℃,提取时间7h。最佳提取条件下,黄酮苷元黄芩素和汉黄芩素的提取率分别为175．04、28．84μmol/g（药材）,分别为传统水煎煮法的6．7倍和4．8倍。[结论]纤维素酶辅助提取法提取黄芩素和汉黄芩素等转化率高,易于实现规模化生产。     

商洛黄芩汉黄芩素提取及复合保鲜液应用研究

本文以商洛黄芩为实验材料,采用超声辅助乙醇提取黄芩中汉黄芩素,研究超声时间、超声提取温度、超声功率、料液比和乙醇体积分数等因素对汉黄芩素提取率的影响,在单因素试验的基础上,设计3因素3水平响应面试验,分析各因素的显著性和交互作用,确定的优化工艺为：在超声功率140W、料液比1:20、乙醇体积分数60%前提下,黄芩汉黄芩素提取率为0.526%。将汉黄芩素提取液与1.5%壳聚糖、1%柠檬酸进行复配,制备复合保鲜液,以感官指标、pH值、菌落总数为检测指标,模拟易导致新鲜草莓变质的温度和湿度进行保藏试验,通过对草莓品质的评价说明不同浓度黄芩汉黄芩素复合保鲜液的保鲜效果,结果表明：0.20%复合保鲜液即可以保藏新鲜草莓3d,0.40%复合保鲜液能够有效地保藏新鲜草莓5d。由于试验模拟草莓极易腐败变质的温度,可以预测室温下草莓贮藏期更长。本研究结果可作为商洛地区黄芩中汉黄芩素应用的技术参考。