| 几种茄属植物基因组DNA提取与RAPD分析 |
| |
| 引用本文： | 柳李旺,汪隆植.几种茄属植物基因组DNA提取与RAPD分析[J].安徽农业科学,1997,25(3):193-195. |
| |
| 作者姓名： | 柳李旺 汪隆植 |
| |
| 作者单位： | 南京农业大学园艺系 |
| |
| 摘 要： | 研究了适于茄子及其近缘野生种基因组ＤＮＡ的提取方法，并对ＤＮＡ初步进行了ＲＡＰＤ分析。结果表明：ＳＤＳ－氯仿－异戊醇法不适于茄属植物ＤＮＡ提纯，而改进的ＣＴＡＢ－酚－氯仿－异戊醇对其成株叶／芽即可获得较高产率及纯度的ＤＮＡ；ＰＣＲ反应扩增出不同片段，引物ＯＰＧ—１１扩增片段在栽培品种与野茄中无差异，而在红茄与龙葵中呈现出一定的多态性。
|
| 关 键 词： | 茄子 近缘野生种 基因组DNA RAPD分析 |
Genomic DNA Extraction and RAPD Analysis of Eggplant and Its Wild Relatives |
| |
| Liu Liwang et al.Genomic DNA Extraction and RAPD Analysis of Eggplant and Its Wild Relatives[J].Journal of Anhui Agricultural Sciences,1997,25(3):193-195. |
| |
| Authors: | Liu Liwang |
| |
| Abstract: | The efficient Genomic DNA extraction of eggplant(Solanum melongena) and its wild relatives and RAPD analysis were preliminarily studied.The results showed the improved CTAB_method was much better than SDS_method in extracting genomic DNA of Solanum plants.Using primer OPG_11,genomic DNA were amplified and exhibited certain polymorphism,but between S. incanum and three cultivars, the difference in amplified fragments was not found.The amplified fragments showed much polymorphism between S.integrifolium,S.incanum and S.nigrum. |
| |
| Keywords: | Eggplant Wild relatives DNA extraction RAPD |
| 本文献已被 CNKI 维普 等数据库收录！ |
