竹材木质素选择性降解菌株的分子鉴定

鉴定1株从腐竹中筛选的高效选择性降解竹材木质素的优良菌株ZNLD-18，为生物法提取可纺性竹原纤维奠定基础。利用真菌通用引物ITS1和ITS4扩增并测定菌株的rDNA ITS区序列，序列总长541bp。把所得序列在GenBank中进行B1ast搜索得到同源性较高的同属不同种菌株序列。比较这些序列的遗传距离，显示菌株ZNL D～18与Trametes versicolor的ITS区序列同源性为99％以上。利用PAUP软件构建分子系统发育树，通过对该树的分析并综合菌株的形态特征，鉴定菌株ZNLD-18为白腐菌Trametes versicolor。

Molecular identification of the strain ZNLD-18 selectively decomposing bamboo lignin

This objective was to provide basis for the biological extracting of the spinning bamboo fiber. The strain ZNLD-18, separated from decaying bamboo, which was able to decompose selectively the bamboo lignin, was identified by polymerase chain reaction （PCR）. The rDNA ITS sequences of the strain were amplified and tested by the fungi universal primers ITS1 and ITS4. The sequence length was 541 base pair （bp）. The sequences were Blast searched within the GenBank to obtain the different strains＇ sequences with the similar homology. The comparison of the genetic distances of these sequences showed that the ITS sequences of ZNLD-18 and Trametes versicolor were with over 99% homology. A molecular phylogenetic tree was built with Phylogenetic Analysis Using Parsimony （PAUP） software. Given the analysis results of the tree and the morphological characteristics of the strain, ZNLD-18 was Trametes versicolor.