高效液相色谱法测定氢溴酸槟榔碱的含量

目的]考察建立氢溴酸槟榔碱含量的测定方法。方法]通过考察不同流速和柱温等因素对分离的影响,研究槟榔碱含量测定的最佳方法。结果]采用高效阳离子交换色谱法,流动相为乙腈-0.5%磷酸(50∶50),检测波长215 nm,柱温30℃,流速1.5 ml/min,在上述色谱条件下,槟榔碱的保留时间约为12.6 min,分离度良好。进样量在0.010~0.080 mg/ml浓度范围内,浓度与峰面积呈良好线性关系。该方法在紫外检测下最低能定量检测到500 ng。结论]阳离子交换色谱法操作简便,具有良好的专属性和准确度,可有效控制产品质量,但不适合用来测定药动学试验或药物残留试验中微量槟榔碱的含量。

Content Detection on Arecoline Hydrobromide by HPLC

Objective]The aim was to establish the detection method for the Arecoline hydrobromide content.Method] The optimum detection method of arecoline hydrobromide content was studied through studying the effect of some factors such as different flow rate and column temperature on separation.Result] The high performance cation-exchange chromatography was adopted.Mobile phase was acetonitrile0.5% H3PO4(50∶50),the detection wavelength was 215 nm,the column temperature was 30 ℃ and the flow-rate was 1.5 ml/min.Under the above chromatographic conditions,the separation was good when the retention time of the arecoline was about 12.6 min.When the concn.of the injection volume was 0.010～0.080 mg/ml,there was a good linear relationship between the concn.and the peak area.The method could detect 500 ng quantitatively under the ultraviolet detection.Conclusion] The operation of cation-exchange chromatography method was convenient,and this method had good specialization and accuracy,could control the production quality effectively,but it was not suitable for the detection on micro arecoline content in the pharmacokinetics experiment or residue experiment.