| RP-HPLC法测定颈复康颗粒中葛根素的含量 |
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| 引用本文： | 李红芳,韩继永.RP-HPLC法测定颈复康颗粒中葛根素的含量[J].安徽农业科学,2012,40(10):5883+5894. |
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| 作者姓名： | 李红芳 韩继永 |
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| 作者单位： | 江苏联合职业技术学院连云港中医药分院,江苏连云港,222006;江苏奥赛康药业有限公司,江苏南京,211112 |
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| 摘 要： | 目的]采用反相高效液相色谱法测定颈复康颗粒中葛根素的含量。方法]色谱柱为Kromasil 100-5C18(250 mm×4.6 mm,5μm);流动相为无水甲醇-水-磷酸(20∶80∶0.2,V/V/V),流速为1.0 ml/min;检测波长为250 nm。结果]阴性对照无干扰;计算回归方程为Y=4 233 801X-34 587,R为0.999 9(n=6)。结果表明,在0.063～1.254μg/ml浓度范围内,葛根素浓度与峰面积呈良好的线性关系,R=0.999 9;加样回收率为101.8%,RSD为1.09%(n=9)。结论]该方法简便、准确、专属性强,可用于颈复康颗粒中葛根素的含量测定。
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| 关 键 词： | HPLC 颈复康颗粒 葛根素 含量 |
The Determination of the Content of Puerain in Neck-Recover-Granule Using RP-HPLC |
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| Institution: | LI Hong-fang et al(Lianyungang TCM Higher Technical Institute,Jiangsu Union Technical Institute,Lianyungang,Jiangsu 222006) |
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| Abstract: | Objective] The aim was to determine the content of puerarin in neck-recover-granule using RP-HPLC.Methods] The chromatographic column was Kromasil 100-5C18(250 mm×4.6 mm,5 μm);mobile phase was absolute methanol-water-phosphoric acid(20∶ 80∶ 0.2,V/V/V),flow velocity was 1.0 ml/min;the detection wavelength was 250 nm.Result] The negative control showed no interference,the calculated regression equation was Y=4 233 801X-34 587,R=0.999 9(n=6).The results showed that within the concentration of 0.063-1.254 μg/ml,there was good linear relation between puerarin concentration and peak area,R=0.999 9;the recovery rate was 101.8% and RSD was 1.09%(n=9).Conclusion] The method is simple,convenient and accurate,has strong specificity,can be used to determine the content of puerarin in neck-recover-granule. |
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| Keywords: | HPLC Neck-recover-granule Puerain Content |
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