| 海南黄灯笼辣椒HPLC特征图谱研究 |
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| 引用本文： | 李国伟,纪明慧,郭飞燕,杨淑玉,李钻丹.海南黄灯笼辣椒HPLC特征图谱研究[J].安徽农业科学,2011,39(15):8840-8843. |
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| 作者姓名： | 李国伟 纪明慧 郭飞燕 杨淑玉 李钻丹 |
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| 作者单位： | 海南思坦德生物科技有限公司,海南海口,570314;海南师范大学省部共建-热带药用植物化学教育部重点实验室,海南海口,571158 |
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| 基金项目： | 海南省教育厅科研基金项目(Hj2010-19) |
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| 摘 要： | 目的]建立黄灯笼辣椒的高效液相色谱特征图谱,为科学评价和有效控制海南黄灯笼辣椒提供新方法。方法]收集6个产地2个季节的黄灯笼辣椒,采用高效液相色谱测定其特征图谱。色谱条件：十八烷基硅烷键合硅胶为填充剂,检测波长281 nm,以甲醇-水-磷酸（70.0∶30.0∶0.1）为流动相,流动速度1.0 ml/min。结果]初步建立了黄灯笼辣椒的高效液相色谱特征图谱。结论]高效液相色谱特征图谱重现性好,可作为黄灯笼辣椒的一项质控指标。
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| 关 键 词： | 黄灯笼辣椒 辣椒碱 高效液相色谱法 特征图谱 |
Study on HPLC Specific Chromatogram of Capsicum chinense Jacq.in Hainan Province |
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| LI Guo-wei et al.Study on HPLC Specific Chromatogram of Capsicum chinense Jacq.in Hainan Province[J].Journal of Anhui Agricultural Sciences,2011,39(15):8840-8843. |
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| Authors: | LI Guo-wei |
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| Institution: | LI Guo-wei et al(Hainan Standard Biological Technology Co,Ltd,Haikou,Hainan 570314) |
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| Abstract: | Objective]The aim was to establish HPLC specific chromatogram for the scientific evaluation and effective identification of Hainan Capsicum chinense Jacq.Method]These Hainan Capsicum chinense Jacq were collected in two seasons in six regions respectively.And their fingerprints were examined by high-performance liquid chromatography.Chromatography conditions:HPLC was performed on C18 column with methanol-water-phosphoric acid(70∶ 30∶ 0.1) as mobile phase at flow rate of 1.0 ml/min.The detection wavelength was 281 nm.Result]The HPLC specific chromatogram of Hainan Capsicum chinense Jacq.was established preliminarily.Conclusion]HPLC specific chromatogram method was repeatable and could be used in quality assessment of Hainan Capsicum chinense Jacq. |
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| Keywords: | Capsicum chinense Jacq Capsaicin HPLC Specific chromatogram |
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