极小种群植物“川柿”生物学特性及种子萌发方法研究

摘 要：【目的】为了保护和发展极小种群植物川柿，开展此研究。【方法】采用观测法，连续3年对其生物学特性研究；种子萌发方法：（1）后熟0、30、45、60天，剥离清洗播种；（2）后熟60天，以GA300、600、1000 mg/L溶液浸种1h；播种基质为河沙（保持一定湿度），放置室内，统计萌发时间和出苗率。【结果】（1）川柿花期4-5月，10月为果熟期，单果直径32.7±5.80mm，单果重23.0±5.30g；种子肾形、千粒重1070g；成年植株呈隔年开花、结果现象；（2）种子经“后熟+河沙播种”，可萌发，其中以后熟60天的出苗率较高，为65%；直接播种种子，出苗率5%；“后熟+GA溶液浸种+河沙播种”，出苗率均高于65%，其中以GA600mg/L浸泡1h处理，出苗率最高为83.3%。【结论】极小种群植物川柿为常绿叶乔木，有隔年开花、结果现象； “果后熟60天+GA600mg/L浸泡种子1h +室内河沙播种”可提高种子萌发率，为川柿种子繁殖提供参考。     

Sacred sites as habitats of culturally important plant species in an Indian megacity

Cultural ecosystem services related to urban green spaces contribute significantly to liveable cities. While previous studies highlight the intersection of cultural ecosystem services with societal values, spiritual or religious values associated with urban nature have received less attention. In India, as in other parts of the world, sacred sites are known for their biological richness, but analyses from urban sacred sites are largely missing. Based on a stratified random sampling approach, we analysed the cultivated and wild plant species assemblages of 69 sacred sites in the megacity of Bengaluru, India, in relation to biological and cultural features, and parameters related to the urban matrix and type of sacred sites (temple vs. katte). Unlike other urban studies, we found a dominance of native species in the cultivated and spontaneous species pools (121 species in total), with Ficus religiosa and Azadirachta indica as most frequently planted species. Culturally relevant species prevailed in the species pool (89%), with overlaps between religious (36%), medicinal (50%) and ornamental (62%) plants; only 11% of species were weeds. Urban matrix parameters (age of development, housing density) and size and type of sacred sites were related to differences in species assemblages. We identified key species for different classes of age and housing density, and for types of sacred sites. Our study demonstrates that urban sacred sites have an important potential in harbouring both native and culturally significant species that can support urban livelihoods in developing countries by a range of cultural and provisioning ecosystem services, including medicinal uses. As such sites are conserved by communities for spiritual or cultural beliefs, local biodiversity can be enhanced, e.g. by adapting management practices through community participation. This would strengthen the important contribution of sacred sites within the green infrastructure of rapidly growing megacities.     

果蔬型甜玉米新品种京科甜191的选育

京科甜191是以超甜玉米自交系T68为母本,自交系T6302为父本,通过杂交选育而成的果蔬型甜玉米品种。果穗筒型,穗长19.0 cm,穗粗5.3 cm,穗行数16~18行,行粒数36~38粒,粒行整齐。籽粒亮黄色,外观漂亮。平均每667 m2鲜果穗产量900 kg左右,出籽率约67.4%。鲜籽粒含粗蛋白2.98%,粗脂肪1.37%,总糖6.7%,口感甜脆清爽,皮薄无渣。播种至采收平均85.4 d(天),适宜京津冀及类似生态区种植。     

马铃薯AP2/ERF转录因子的克隆及植物表达载体构建

AP2/ERF基因家族转录因子普遍存在于植物中,参与植物体内的各种生物学过程,包括植物的生长发育、生物和非生物胁迫响应等。前期转录组测序的结果发现马铃薯‘加湘1号’一个AP2/ERF家族基因(PGSC0003DMG400012154)在接种晚疫病菌(Phytophthora infestans)24 h后被显著激活。从接种P.infestans 24 h的‘加湘1号’的总RNA中通过RT-PCR获得了该基因CDS序列为885 bp,BLAST分析显示该基因编码一个295个氨基酸残基的蛋白,并且含有1个AP2/ERF结构域,是AP2/ERF转录因子家族中的ERF亚家族的一员。本研究将该基因与XcmⅠ酶切的表达载体pCXSN连接,转化大肠杆菌,通过测序挑选插入正确克隆酶切验证,并成功转化农杆菌。本研究结果为进一步研究该基因的功能提供了帮助。     

不同氮素水平下双季稻株型与冠层内光截获特征研究

本文旨在阐明双季稻株型与冠层内光合有效辐射截获的时空分布特征。选用4个不同株型早、晚稻品种,设置4个不同施氮水平,系统观测其植株形态和冠层内光合有效辐射截获率(IPAR)的时空分布状况。结果表明,施氮水平对早、晚稻株高、穗长、叶长和叶基角均有显著影响,均表现为随施氮水平的增加而增大;早、晚稻孕穗期的分层叶面积指数(LAI)和向上累积LAI大于抽穗后12 d,分层LAI呈冠层中部大于上部和下部的分布特征,最大分层LAI出现在0.58相对高度处;冠层上中部分层LAI和向上累积LAI随施氮水平的增加而增大;向上累积LAI随相对高度呈S型曲线分布,可用Logistic方程定量描述(R~2 0.99);早、晚稻孕穗期的冠层IPAR大于抽穗后12 d,且随施氮水平的增加而增大,其日变化表现为正午较小,早晚较大;株型紧凑的早、晚稻品种,冠层IPAR低;冠层IPAR与向下累积LAI之间的关系可用方程IPAR=a (1-e~(-b×LAI))定量描述(R~2 0.88);冠层内IPAR的三维空间分布表现为冠层上中部水平面上IPAR较低,光斑变化大,冠层下部水平面上IPAR较高,光斑变化较平缓,同一冠层高度水平面上的IPAR呈不均匀分布。研究结果可为双季稻高产栽培及理想株型的优化设计提供支撑。     

中国竹类多样性及其重要价值

竹类属于禾本科Gramineae（Poaceae）、竹亚科Bambusoideae植物，全世界约1 500多种。中国竹类的自然分布广阔，横跨热带、亚热带和温带地区，涵盖包括台湾、香港在内的21个有竹子分布的省、自治区、直辖市和特区。中国是世界上竹类多样性最丰富的国家，也是世界上栽培竹类最丰富的国家，共有竹类植物753种（含22种引进种），约占世界总数的50%以上。同时，中国竹类在起源、遗传进化、内部构造、生态及林型等方面均具有十分丰富的多样性。中国竹类从宏观到微观所展现出的多样性特质，是大自然留给人类的宝贵遗产，对于人类认识自然、保护自然、从而更好的利用竹类资源服务于人类的长远利益，具有重要价值。文章从起源、生态、林型、物种、形态、遗传等方面概述了中国竹类的多样性，为进一步探讨竹类多样性提供基础信息。     

马疱疹病毒1型主要毒力基因分析及TK基因缺失载体的构建

为了解新疆马疱疹病毒1型（EHV-1）主要毒力基因遗传进化情况并构建TK基因缺失株，本研究以EHV-1 XJ2015株DNA为模板，对其主要毒力基因TK、gI和gE全长进行克隆、测序及生物信息学分析，并扩增TK基因左右重组臂TKL和TKR，构建质粒pUC-TKLR，将扩增后的增强绿色荧光蛋白（EGFP，含有CMV+polyA）插入pUC-TKLR质粒，构建TK基因缺失打靶质粒。TK、gI和gE基因同源性分析结果显示，XJ2015株与国外EHV-1分离株TK、gI和gE基因同源性均较高，分别为99.8%~100.0%、99.6%~100.0%和99.9%~100.0%；与EHV-3分离株同源性均最低，分别为72.9%、59.4%和62.1%；遗传进化分析显示，3个基因均与国外EHV-1同属于一个遗传进化分支，与EHV-9和EHV-4进化关系较近，但与EHV-3进化关系较远，表明XJ2015毒株与国外EHV-1毒株TK、gI、gE基因核苷酸上差异不明显，没有明显的地域性特征，功能基因保守且进化缓慢，同源基因功能相同或相近；经PCR扩增、酶切、测序及转染鉴定，本试验成功构建了用于TK基因缺失的打靶质粒pUC-TKLR-EGFP。通过对EHV-1主要毒力基因的分析及TK基因缺失打靶载体的构建，为新疆地区马鼻肺炎流行病学调查分析、TK基因缺失株的构建提供理论依据。     

Influencing in vitro clonal propagation of Chonemorpha fragrans(moon) Alston by culture media strength,plant growth regulators,carbon source and photo periodic incubation

Chonemorpha fragrans is an endangered medicinal woody climber,regarded among alternative plant sources of camptothecin.Camptothecin is a monoterpene indole anti-cancer alkaloid with annual trade value of over three billion U.S.dollars in the recent,and is used in the production of its analog drugs approved for the chemotherapy of cancer of varied types.Effects of plant growth regulators,culture media strength and photoperi-odic duration on the micropropagation ef ciency of C.fragrans from nodal segment explants were studied on Murashige and Skoog(MS)medium amended with Thidiazuron(TDZ),Benzylaminopurine(BAP)or Kinetin(Kin).Thidiazuron was more ef cient over BAP and Kin when half basal MS medium was used over full or quarter strength.Results of carbon source experiment showed sucrose as the most effective over glucose,fructose,and maltose in the clonal production.Studies on the photope-riodic incubation duration showed 12 h as the best light period and sub or supra-optimal resulted in the production of abnormal and albino micro shoots.Experimental results on the evaluation of physiological,biochemical parameters showed the role of pigment molecules and antioxidant systems in the production of albino micro shoots.     

具有III型分泌系统的Pseudomonas moraviensis FP1761植物益生功能及其全基因组分析

 本研究从节瓜根际分离到一株具有防病促生功能的荧光类假单胞菌FP1761。该菌株对部分植物病原真菌和细菌具有拮抗能力,能够解钾、解有机磷和无机磷,可产生氨、蛋白酶、嗜铁素、吲哚乙酸。生物测定表明菌株FP1761可显著促进小麦生长。生理生化、平均核苷酸相似度、16S rDNA和多基因分析将FP1761鉴定为摩拉维亚假单胞菌(Pseudomonas moraviensis)。菌株FP1761基因组草图全长6.12 Mb,(G+C)含量为59.9%,共编码5467个基因序列。将该菌株与种内3个代表性菌株进行泛基因组和核心基因组分析,共产生 4 357个共有基因,菌株FP1761特有基因327个。利用antiSMASH对菌株次生代谢基因簇进行预测,发现其含有8个潜在的次生代谢产物基因簇。其中两个基因簇与嗜铁素pyoverdine合成相关,未见聚酮类合成基因。基因组分析发现,该菌株具有与病原性假单胞菌相似的III型分泌系统,但丢失了效应蛋白调控因子hrpS和转运相关的hrpH和hrpK1基因。对全基因组扫描,菌株FP1761仅保留了病原性假单胞菌的保守效应蛋白AvrE和HopAA1-1。FP1761是目前已发现的唯一具有III型分泌系统的摩拉维亚假单胞菌。本研究表明摩拉维亚假单胞菌FP1761具有潜在的植物防病促生功能,但其III型分泌系统与植物益生互作机制有待进一步解析。