杂交早稻新组合顺优109种植表现及高产栽培技术

顺优109是早稻早熟新组合,2009年通过广西农作物品种审定,2011年在灵川县潭下镇香殿村连片种植7.67hm2,加权平均产量8.398t/hm2,最高产量8.727t/hm2,表现出高产、稳产、早熟、抗性好等特性。本文详细介绍其种植表现及高产栽培技术。     

两种柳树无性系幼苗生长和光合特性研究

为进一步比较不同柳树无性系幼苗生长及光合特性的差异,选择优良柳树无性系,以垂爆109柳SalixבChuibao 109’和旱快柳Salix matsudana var.anshanensis为试材,采用Hoagland营养液水培方法,研究正常情况下垂爆109柳和旱快柳幼苗生长性状和光合特性。结果表明:培养45d后,与旱快柳相比垂爆109柳幼苗的叶片数、叶长、根长、根数、基径无显著差异;垂爆109柳的叶宽、叶间距、新枝长、枝条鲜质量和干质量,根鲜质量和干质量,都明显高于旱快柳(p<0.01)。垂爆109柳和旱快柳的净光合速率有明显的差异,净光合速率值在不同光照强度下均表现为垂爆109柳>旱快柳。垂爆109柳的光饱和点和光补偿点都低于旱快柳,表明旱快柳具有较强的光强利用能力,强光照射对旱快柳的抑制作用较低;而垂爆109柳利用弱光的能力较强。     

BHBA对HT22细胞中ACE表达的影响

生酮饮食(KD)已被证实具有神经保护作用,但其机制尚不明确。本试验以小鼠海马神经元HT22为细胞模型,利用2 mmol/Lβ-羟基丁酸酯(BHBA)对HT22细胞进行处理,通过荧光定量PCR,免疫蛋白印迹和信号通路阻断试验,观察BHBA对HT22细胞ACE表达的影响,并探讨参与调控的信号通路。结果表明,2 mmol/L BHBA可通过激活HT22细胞内GPR109A受体和ERK1/2信号通路进而增加HT22细胞中ACE的表达。     

高毒力苏云金杆菌CAB109菌株的蛋白质特性及其田间防效

对甜菜夜蛾等害虫具有高毒力的苏云金杆菌CAB109菌株进行生物学特性调查结果表明,CAB109菌株的伴孢晶体具有典型的菱形结构;其原毒素蛋白的分子量为133kDa,经胰蛋白酶处理后水解为66kDa的活性毒素蛋白．DNA凝胶电泳显示：CAB19菌株的质粒DNA与同样H,血清型的已知HD-133菌株不同．CAB109菌株对葱田甜菜夜蛾的平均防效达75．3％,高于常用化学农药和微生物农药的防效;平均挽回损失率也达26．0%表现出优良的开发潜力．     

Inhibitory effect of polypeptide from Buthus martensii Karsch on neoplasm cells

AIM: To observe the inhibitory effects of polypeptide from Buthus martensii venom (PBMV) on human tumor cell lines and the influence of PBMV on cell cycle migration, expression of tumor-suppressor gene p53 and the membrane potential of CNE-2Z cells. METHODS: MTT colorimetric method, the colony formation and mitosis index, flow cytometry assay and intracellular recording with glass microelectrodes were used. RESULTS: PBMV had obvious cytotoxicity on several tumor cell lines. The cells grow was inhibited by PBMV, colony formation rate and mitotic index of tumor cells were reduced and the number of polykaryocyte was decreased. CNE-2Z cells in S phase were reduced evidently after they were treated with PBMV (P<0.01). After CNE-2Z cells were incubated with PBMV, P53 protein expression in the cells decreased obviously. Differences were very distinct (P<0.01). The negative potential of cell membrane reduced evidently and cell membrane was in depolarization state. CONCLUSION: PBMV probably possesses the effects of the "membrane toxin". By decreasing the membrane potential, the growth and proliferation of tumor cells were interfered by PBMV.     

奶牛GPR109A基因SYBR GreenⅠ实时荧光定量PCR检测方法的建立

[目的]建立奶牛G蛋白偶联受体109A基因(GPR 109A)的实时荧光定量PCR检测方法,为开展奶牛产后脂类代谢紊乱及酮病发生机理研究奠定基础.[方法]根据GenBank中奶牛GPR 109A基因和卢-actin基因(内参基因)的mRNA保守序列设计合成两对引物,以奶牛肝脏组织cDNA为模板,PCR扩增目的基因后与载体连接构建重组质粒,以SYBR Green Ⅰ实时荧光定量PCR进行扩增,绘制标准曲线,并对其特异性、敏感性、重复性等进行检验.[结果]GPR109A基因和β-actin基因的实时荧光定量PCR标准曲线方程分别为y=-3.309x+10.92(R2=0.9985)和y=-3.289x+9.794(R2 2=0.9997),扩增效率分别为101.0％和100.0％.特异性试验结果显示,GPR 109A基因和β-actin基因的溶解曲线峰单一,无引物二聚体和非特异性扩增产物生成;敏感性试验结果显示,线性扩增范围广,可检测到1.8×102个拷贝的GPR109A基因;重复性试验结果显示,各扩增反应的变异系数(CV)小于或等于1.7％.[结论]基于GPR109A基因建立的SYBR Green Ⅰ实时荧光定量PCR具有操作简便、敏感性高、特异性强和重复性好等特点,可用于牛源性GPR109A基因表达量的快速检测和定量分析.     

Effect of DEC1 gene over-expression on proliferation and invasion abilities of human esophageal cancer ECA109 cells

AIM: To investigate the effect of DEC1 gene over-expression on the proliferation and invasion abilities of human esophageal cancer ECA109 cells.METHODS: ECA109 cells were transfected with plasmid pcDNA3.1 (-)/DEC1 (DEC1 group) or pcDNA3.1 (-) (vector group). The mRNA and protein levels of DEC1, cyclin D1 and MMP-9 were evaluated by real-time PCR and Western blot, respectively. The effects of DEC1 over-expression on the proliferation and invasion abilities of the ECA109 cells were evaluated by CCK-8 assay, colony formation assay and Transwell test respectively.RESULTS: The DEC1 expression level in ECA109 cells in DEC1 group was significantly higher than that in vector group (P<0.01), but the levels of MMP9 and cyclin D1 expression were opposite (P<0.01). However, both the proliferation and invasion abilities of ECA109 cells in DEC1 groups decreased significantly as compared with those in vector group (P<0.05).CONCLUSION: The over-expression of DEC1 significantly inhibits the proliferation and invasion of ECA109 cells, which may be involved in the expression of cyclin D1 and MMP9.     

两系杂交粳稻‘云光109’选育与繁殖制种关键技术研究

‘云光109’是利用光敏核不育系(PGMS)‘N95076S’与恢复系云粳恢7号组配而成.具有高产(9.0 ～ 13.97 t/hm2)、感稻瘟病(9级),抗白叶枯病(3级).米饭食口性好、米质优、经检测属优质软米杂交粳稻.2011年同时通过云南省和贵州省农作物新品种审定,编号分别为滇审稻2011008号和黔审稻2011011号;2012年通过农业部超级稻认证.通过2008年至2012年‘云光109’大面积制种实践表明,‘N95076S’在湖北省仙桃市最佳抽穗扬花期安排在8月16日至8月24日.此时阳光充足、温度高使其容易通过育性转换敏感期,且不育性稳定,确保了杂交种子的纯度,以此确定最佳播种期为6月4日.在仙桃‘N95076S’不育系在9月22日左右转换为可育,因此,对‘云光109’进行繁种需在7月14日播种,9月16日实现抽穗.同时,‘云光109’经过一季制种后,可以通过再生繁殖,实现一季播种制种、繁殖双季,从而实现高产、优质、高效益.     

转OsbHLH1和Bar基因水稻及相关特性分析

OsbHLH1基因编码bHLH(basic helix-loop-helix,碱性螺旋-环-螺旋)类转录因子,与水稻耐低温相关。草铵膦是一种高效低毒灭生性除草剂,Bar基因表达产物能解除草铵膦的毒性。本实验对通过农杆菌(Agrobacterium tumefaciens)浸染法获得的以粳型水稻(Oryza sativaL.subsp.japonica Kato)恢复系淮C17为受体的转OsbHLH1和Bar基因水稻进行研究,鉴定其除草剂抗性和耐低温能力,考查其农艺性状。草铵膦筛选发现Bar基因正常表达;对草铵膦筛选获得的转基因植株进行PCR、Southern、RT-PCR和实时荧光定量PCR检测,发现OsbHLH1基因已经整合到水稻基因组中并且在水稻中表达。转基因T3代株系芽期(萌发芽长0.5mm)在2℃条件下处理6d,第6号转基因株系死苗率为17.8%,对照死苗率为61.1%。T3代苗期(一叶一心)在8～10℃下处理7d,第5、6、9、11号株系的根长显著长于对照,第3、5、11号株系的根数显著多于对照,第3号株系的平均鲜重显著重于对照。芽期、苗期耐冷性试验表明,OsbHLH1基因在水稻中过量表达显著提高了水稻芽期、苗期的耐低温能力。对转基因T2代的农艺性状进行考查,株高、千粒重、结实率、理论产量等农艺性状与对照相比较均出现了显著的变化,外源基因的导入对水稻的农艺性状有明显影响。研究结果将为选育抗除草剂、耐低温杂交粳稻新组合提供新种质。     

烟酸调节动物肠道黏膜屏障功能的分子机制

作为一种重要的维生素,烟酸在抑制肠道炎症反应及维持肠道健康等方面发挥重要作用。研究表明,烟酸可以直接结合烟酸受体G蛋白偶联受体109A(GPR109A)发挥其生理作用,还能够以辅酶烟酰胺腺嘌呤二核苷酸(NAD^+)的形式参与细胞能量代谢。本文主要阐述了烟酸维护肠道黏膜屏障功能的作用机制,为维护断奶仔猪肠道健康提供科学理论依据。