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Brett Glencross Wayne Hawkins David Evans Neil Rutherford Peter McCafferty Ken Dods Sofie Sipsas 《Aquaculture (Amsterdam, Netherlands)》2008,277(3-4):251-262
A series of studies were designed to examine the degree of variability in the digestibility of protein and energy from lupin (Lupinus angustifolius) kernel meals when fed to rainbow trout (Oncorhynchus mykiss) and the potential implications of this variability. The digestibility of protein and energy from 10 different varieties of lupin kernel meal was assessed where the test ingredient comprised 30% of each test diet. Digesta was collected using faecal stripping techniques. Digestible protein value ranged from 331 to 508 g/kg DM and digestible energy values ranged from 10.6 to 13.3 MJ/kg DM. To examine the implications of variability in digestible protein and energy value, two lupin kernel meals from the extremes of the protein digestibility range (Lupin-1: ADN ~ 70% and Lupin-2: ADN ~ 100%) were chosen for assessment in two growth studies. Soybean meal and a reference diet with fishmeal as the only protein source were also included in the study. In the first growth experiment the test ingredients were included at equal concentrations (40%) in protein-limiting diets (350 g protein/kg DM) and fed at either of two ration levels (restricted and satiety). Diets were formulated on a crude basis so as to place the test variable on the variability in digestible protein value of the diets. In the restricted-fed treatments growth of fish fed the reference diet was highest, but not significantly better than lupin-H. Growth of fish fed the lupin-L diet was significantly poorer than both the reference and lupin-H diets, but not poorer than the soybean diet. The growth responses observed from this experiment clearly showed that the differences in feed intake and/or digestible protein value could be demonstrated in terms of significant differences in growth outcomes. In a second growth study high-nutrient dense extruded diets (400 g protein/kg and 23.5 MJ/kg) were prepared with a more practical level of 25% inclusion of the same test materials. Again the diets were formulated on crude basis so as to place the test variable on the variability in digestible protein and energy value of the diets. Growth of fish restrictively fed the lupin-H diet was highest, but not significantly better than the soybean, reference or lupin-L treatments restrictively fed. Growth of fish satietal fed the soybean diet was significantly poorer than the reference and lupin-H diets, but not compared to the lupin-L diet. The growth responses observed from this experiment showed that the differences in digestible protein and energy value could not be demonstrated in terms of significant differences in growth outcomes, and that feed intake variability and excess nutrient supply masked the effect of this variability; particularly at the satietal feed intake levels. 相似文献
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茶树菇深层发酵产物与子实体营养成分的分析 总被引:1,自引:0,他引:1
对茶树菇深层发酵产物和子实体的一般营养成分、氨基酸的组成及含量、微量元素的含量以及不同来源的茶树菇多糖的单糖组成进行了分析.结果表明,茶树菇发酵菌丝体、发酵液和子实体均合有17种氨基酸,以谷氨酸和天冬氨酸含量最高,必需氨基酸中茶树菇菌丝体和子实体都以亮氨酸含量最高,发酵液以赖氨酸含量最高.茶树菇菌丝体和子实体中均含有较高的Ca、Fe、Zn等人体易缺少的微量元素,菌丝体中的微量元素含量的多少易受培养基成分的影响,与子实体中微量元素含量有差别.胞内粗多糖中多糖含量为11.2%,胞外粗多糖中多糖含量为20.3%.不同来源的茶树菇深层发酵多糖在单糖组成上基本相似,都是以葡萄糖作为其主要成分,其次是半乳糖,除了5种标准单糖外,可能还含有其他单糖.茶树菇胞内多糖和胞外多糖各主要单糖组成及质量分数分别为阿拉伯单糖0.74%、1.10%,木糖1.41%、0.62%,甘露糖0.96%、1.30%,葡萄糖22.3%、26.3%,半乳糖2.97%、3.15%. 相似文献