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1.
Saori MIURA Noriyuki HORIUCHI Kotaro MATSUMOTO Yoshiyasu KOBAYASHI Shin-ichiro KAWAZU Hisashi INOKUMA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(7):883-887
Monoclonal integration of bovine leukemia virus (BLV) proviral DNA into bovine genomes
was detected in peripheral blood from two clinical cases of enzootic bovine leukosis (EBL)
without enlargement of superficial lymph nodes. A BLV-specific probe hybridized with 1 to
3 EcoRI and HindIII fragments in these 2 atypical EBL
cattle by Southern blotting and hybridization, as well as in 3 typical EBL cattle. The
probe also hybridized to a large number of EcoRI and
HindIII fragments in 5 cattle with persistent leukosis. These results
suggest that the detection of monoclonal integration of BLV provirus into the host genome
may serve as a marker of monoclonal proliferation and malignancy in difficult to diagnose
EBL cattle. 相似文献
2.
Kazuhiko ARAHARA Takashi MATSUMOTO Fumiki MORIMATSU Katsuhiko ARAI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(11):1503-1506
In this study, the effect of fibronectin on the neurite outgrowth from embryoid
bodies (EBs) in neurodifferentiated embryonal carcinoma P19 cells was examined. The
neurite outgrowth on fibronectin was maintained for a longer time in comparison with those
on collagen or laminin. Quantitative RT-PCR revealed that mRNA level corresponding to
sonic hedgehog (Shh) in neurodifferentiated P19 cells was upregulated on fibronectin,
whereas collagen or laminin did not affect. Further knockdown of integrin αv subunit in
P19 cells demonstrated that expression of Shh was mediated through interaction between
fibronectin and integrin. Additionally, exogenous Shh agonist accelerated neurite
outgrowth from embryonic stem cell-derived EBs without large change of neuronal phenotype
expression. Taken together, fibronectin could maintain neurite outgrowth via increased Shh
expression. 相似文献
3.
This study was undertaken to investigate genotypic differences of five maize cultivars in grain yield response to two different modes of deficit irrigation, conventional deficit irrigation and partial root zone irrigation. Three irrigation treatments were implemented: (1) FULL irrigation, the control treatment where plant water requirement, 100% Class-A pan evaporation, was fully met and the furrows on both sides of the plant rows were irrigated; (2) partial root zone irrigation (PRI), 35% deficit irrigation, compared to FULL treatment, was applied in every other furrow thus irrigating only one side of the plant rows. The furrows irrigated were alternated every irrigation; (3) conventional deficit irrigation (CDI), the same amount of water as PRI was applied in furrows on both sides of the plant rows, similar to FULL irrigation treatment. Five maize cultivars (P.31.G.98, P.3394, Rx:9292, Tector and Tietar) showing extreme growth response to water stress were selected out of ten cultivars tested with earlier completed greenhouse-pot experiment. A split-plot experimental design, comprising three irrigation treatments and five maize cultivars with four replicates, was used during two years of work, in 2005 and 2006. Total of nine irrigations, with one-week irrigation interval, were annually applied using a drip-irrigation system. Soil water status was monitored using a neutron moisture gauge, in addition to measuring leaf water potential and above-ground biomass production throughout the growing season. Grain yield and other yield attributes were measured at harvest as well as assessing differences in plant root distributions. Decrease in grain yield and harvest index of the tested cultivars, compared to FULL treatment, was proportionally less under PRI than CDI. Whether or not a significant yield advantage can be obtained under PRI compared to CDI showed significant (P < 0.05) genotypic variability. Tector and Tietar among the tested cultivars of maize showed significantly higher grain yield (P < 0.05) under PRI than CDI. The yield advantage of the genotypes (P.3394 and Tector) under PRI compared to CDI seems related to their enhanced root biomass developed under PRI. 相似文献
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(pp. 17–24)
A trial calculation was performed of the environmental nitrogen-assimilation capacity and the amount of nitrogen input based on various statistical data, which were compiled from each city, town and village in Hokkaido prefecture. The relationship between the excess quantity of nitrogen, after nitrogen input, and the environmental nitrogen-assimilation capacity and the nitrate-nitrogen concentration of the groundwater was considered.
Environmental nitrogen-assimilation capacity = nitrogen output by the crops + acceptable level of residual nitrate in the soil profile.*
* It is calculated by the amount of nitrate precipitation evapotranspiration ×10 mg L−1 .
A trial calculation was performed of the environmental nitrogen-assimilation capacity and the amount of nitrogen input based on various statistical data, which were compiled from each city, town and village in Hokkaido prefecture. The relationship between the excess quantity of nitrogen, after nitrogen input, and the environmental nitrogen-assimilation capacity and the nitrate-nitrogen concentration of the groundwater was considered.
Environmental nitrogen-assimilation capacity = nitrogen output by the crops + acceptable level of residual nitrate in the soil profile.*
- 1)
The average value of the environmental nitrogen-assimilation capacity in Hokkaido Prefecture was observed to be 183 kg ha
−1 . The maximum and minimum values of the environmental nitrogen-assimilation capacity were 308 kg ha−1 and 94 kg ha−1 , respectively. When the average value of the environmental nitrogen-assimilation capacity with respect to main agricultural land use was compared across municipalities, it was largely in the following order · grassland (218 kg ha−1 ), upland (169 kg ha−1 ), and paddy land (157 kg ha−1 ).
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Epizootic hemorrhagic disease virus (EHDV) is a vector-borne disease of ruminants disseminated in the tropic and subtropic
zone of the world. Gazella subgutturosa subgutturosa (goitered gazelle) is native species of Anatolia. Due to risk of extinction, captured gazelles have been taken under protection
in a restricted area at Ceylanpinar state farm. In this study, the presence of EHDV was investigated serologically in 82 goitered
gazelle and 399 cattle. Seropositivity rates in goitered gazelle and cattle were detected as 2.43% and 3.50%, respectively. 相似文献
9.
植物伯克霍尔德菌Burkholderia plantarii是引起水稻秧苗细菌性立枯病的重要病原菌之一,其侵染性、繁殖力及适应性均很强,严重威胁中国水稻生产。文章围绕B.plantarii的发生、危害及致病机理,着重论述了细菌群体感应系统(quorum sensing,QS)的生理功能及其在B.plantarii致病力调控方面的最新研究进展,并进一步从根际微生物互作角度,综述了种间信号分子对病原菌群体淬灭(quorum quenching)的作用机制,同时结合种间信号分子的独特性,展望了其在新型微生物杀菌剂研发中的重要性和应用潜力。 相似文献