生物农药企业技术创新能力评价研究

随着科技和经济的发展,人们对生态环境和生活品质越来越重视.在生物技术推动和社会需求拉动的双重作用下,包括生物农药产业在内的生物产业将成为一个新的主导产业.在我国的生物农药产业起步晚、发展还较落后的背景下,对生物农药企业进行技术创新能力评价,找出薄弱环节和创新瓶颈,对生物农药企业的快速发展有积极的促进作用.为此结合生物农药企业的特点,建立了生物农药企业技术创新能力评价指标体系,然后应用模糊综合评价法建立了技术创新能力评价模型,并给出了实例.     

类酵母共生菌与灰飞虱卵巢发育和产卵量的关系

为明确灰飞虱体内类酵母共生菌(YLS)在生殖发育中的作用,采用血球计数法并结合显微摄像系统,研究了灰飞虱雌虫体内YLS的数量动态及与卵巢发育和产卵量之间的相互关系。结果显示,25℃常温和35℃高温处理的灰飞虱体内YLS数量均随成虫的生长而下降,高温处理4龄若虫可以显著减少成虫体内YLS数量。25℃和35℃处理,灰飞虱卵巢发育级别和其体内YLS数量均存在显著负相关(r=－0.903,P＜0.05;r=－0.970,P＜0.01),而日均产卵量则与其YLS数量呈显著正相关(r=0.725,P＜0.05;r=0.659,P＜0.05)。表明共生菌的存在有利于灰飞虱的卵巢发育与卵子成熟。     

拮抗链霉菌B28 的分类鉴定及其生防作用研究

 An antagonistic Streptomyces strain B28, isolated from the soil collected in Tianmu mountain of Zhejiang Province, was identified as Streptomyces diastatochromogenes by morphological , physiological & biochemical characteristics and 16S rDNA sequence alignment. Antagonistic study indicated that its secondary metabolite toyocamycin had an antifungal activity on the mycelial growth of seven species of plant pathogenic fungi. The results showed that toyocamycin possessed marked inhibitory activity against Rhizoctonia solani and the EC50 value was 0. 58 μg / mL. The effective of toyocamycin against Botrytis cinerea and Fusarium oxysporum f. sp. cucumerinum was much higher than that against Colletotrichum gloeosporioides, the EC50 value of
toyocamycin in inhibiting B. cinerea, F. oxysporum and C. gloeosporioides were 13. 65 μg / mL, 12. 33 μg / mL, and 30. 15 μg / mL respectively.