全文获取类型
收费全文 | 87篇 |
免费 | 6篇 |
专业分类
林业 | 7篇 |
农学 | 1篇 |
6篇 | |
综合类 | 7篇 |
水产渔业 | 13篇 |
畜牧兽医 | 50篇 |
园艺 | 3篇 |
植物保护 | 6篇 |
出版年
2021年 | 7篇 |
2020年 | 4篇 |
2019年 | 1篇 |
2017年 | 5篇 |
2016年 | 4篇 |
2015年 | 9篇 |
2014年 | 6篇 |
2013年 | 6篇 |
2012年 | 4篇 |
2011年 | 4篇 |
2010年 | 2篇 |
2009年 | 4篇 |
2008年 | 4篇 |
2007年 | 4篇 |
2006年 | 6篇 |
2005年 | 4篇 |
2004年 | 5篇 |
2003年 | 4篇 |
2002年 | 4篇 |
2001年 | 1篇 |
1992年 | 1篇 |
1991年 | 1篇 |
1990年 | 2篇 |
1989年 | 1篇 |
排序方式: 共有93条查询结果,搜索用时 15 毫秒
1.
2.
Background
The etiology of canine chronic bronchitis (CB) is not completely understood, although exposure to environmental tobacco smoke (ETS) affects the airway inflammatory responses in some dogs with CB. The mechanism by which this occurs is unknown.Findings
We investigated the concentrations and methylation rates of free-floating DNA fragments in bronchoalveolar lavage fluid (BALF) from dogs with chronic bronchitis. Based on serum cotinine levels, dogs with CB were divided into 2 groups: dogs that either had or had not been exposed to ETS. Our results demonstrated that the total nucleated cell and macrophage numbers increased in BALF of ETS-exposed dogs with CB. There were no significant differences in DNA concentrations and methylation rates in BALF between the 2 groups. However, 3 out of 8 dogs exposed to ETS had high DNA methylation rates in their BALF samples.Conclusion
Our results suggest that ETS exposure leads to epigenetic modifications of cellular components in BALF in dogs diagnosed with CB. 相似文献3.
Yoshiki YAMAYA Toshihiro WATARI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(4):421-425
Canine chronic rhinitis (CR) and bronchitis (CB) are suspected to be allergic diseases.
The present study tested whether dogs diagnosed with CR or CB present an atopic
predisposition based on the ratio of CC chemokine receptor 4 (CCR4)-positive cells among
peripheral blood CD4-positive cells (CCR4/CD4) and the serum levels of allergen-specific
IgE antibodies. We found that most dogs with CR and CB have a possibility of atopic
predisposition, and macrolide therapy constitutes an alternative to corticosteroid therapy
in controlling the clinical signs. 相似文献
4.
Katsushige Shiraki Yoshiki Shinomiya Hirofumi Shibano 《Journal of Forest Research》2006,11(6):439-447
Numerical experiments of soil water movement and bedrock infiltration based on a simplified simulation method were conducted
to analyze watershed-scale rainfall-runoff processes. To verify the model accuracy, it was applied to a Minamitani watershed
(0.45 ha). The simulation was performed with 2.5-m space grids horizontally and five cells vertically. Results of long-term
calculation of this model proved that this simulation model is robust and demonstrated good computational water mass conservation.
Calculation results showed the best agreement with observed hydrographs and the number of groundwater levels simultaneously
when laboratory-tested soil hydraulic characteristics for topsoil were used and infiltration into bedrock was included in
numerical calculations. Numerical experiments show that bedrock infiltration generated a stable base flow and suppressed the
secondary discharge peak. The reproducibility achieved by observed soil hydraulic characteristics with the assumption of bedrock
flow demonstrates the effectiveness of the simulation model used in this article for analyses of watershed-scale soil water
movements. 相似文献
5.
6.
Tamie Noda Koichi Murakami Tetsuo Asai Yoshiki Etoh Tomoe Ishihara Toshiro Kuroki Kazumi Horikawa Shuji Fujimoto 《Acta veterinaria Scandinavica》2011,53(1):38
Salmonella enterica subsp. enterica serovar Enteritidis (S. Enteritidis) was responsible for a worldwide pandemic during the 1980s and 1990s; however, changes in the dominant lineage before and after this event remain unknown. This study determined S. Enteritidis lineages before and after this pandemic event in Japan using multilocus sequence typing (MLST). Thirty S. Enteritidis strains were collected in Japan between 1973 and 2004, consisting of 27 human strains from individual episodes, a bovine strain, a liquid egg strain and an eggshell strain. Strains showed nine phage types and 17 pulsed-field profiles with pulsed-field gel electrophoresis. All strains had homologous type 11 sequences without any nucleotide differences in seven housekeeping genes. These MLST results suggest that S. Enteritidis with the diversities revealed by phage typing and pulsed-field profiling has a highly clonal population. Although type 11 S. Enteritidis may exhibit both pleiotropic surface structure and pulsed-field type variation, it is likely to be a stable lineage derived from an ancestor before the 1980s and/or 1990s pandemic in Japan. 相似文献
7.
Yoshiki Matsushita Shusuke Machida Haruyuki Kanehiro Fumio Nakamura Naoto Honda 《Fisheries Science》2008,74(2):230-235
ABSTRACT: A small number of fishers in Chiba Prefecture of eastern Japan use cotton gill nets to catch Japanese spiny lobster Panulirus japonicus. To examine the advantages of cotton gill nets, we analyzed changes in mesh breaking load of a new cotton gill net used in a fishing operation. A new cotton gill net was also soaked in a seawater tank to simulate ghost fishing conditions. The average mesh breaking load of new cotton mesh was 50.3 N. This value decreased to 19.0 N after 38 days (∼912 h), and after 82 days (∼1968 h) the mesh could be easily torn (breaking load 0.07 N). Under fishing conditions, the cumulative soak time was only 744.4 h over 19 months. The average breaking load at the end of this period was 43.1 N, a strength 86% that of the presoaked mesh. The mesh breaking load of a cotton gill net continuously soaked for 744.4 h was 26.1 N, as estimated from tank experiment data. Thus, a cotton gill net maintains reasonable strength under typical use conditions, but will degrade if lost at sea. 相似文献
8.
The swimming depths of 12 individual Nemopilema nomurai with bell diameters of 0.8–1.6 m were investigated using pop-up archival transmitting tags and ultrasonic pingers, and the
validity of the research method was evaluated. The N. nomurai studied frequently showed vertical movement, with the swimming depth ranging from 0 to 176 m, The mean swimming depths of
most individuals were less than 40 m. The swimming depths of N. nomurai in the northern Japan Sea in the winter were mostly deeper than those of this species in the southern Japan Sea in the autumn.
This result suggests that the range of the depths almost depends on the vertical structure of the ocean. Swimming depths during
the nighttime were significantly deeper than those during the daytime. More specifically, during the daytime, the swimming
depths in the afternoon tended to be shallower than those in the morning, while during the nighttime, the swimming depths
after midnight were deeper than those before midnight. 相似文献
9.
Sex determination in the Drosophila germ line is regulated by both the sex of the surrounding soma and cell-autonomous cues. How primordial germ cells (PGCs) initiate sexual development via cell-autonomous mechanisms is unclear. Here, we demonstrate that, in Drosophila, the Sex lethal (Sxl) gene acts autonomously in PGCs to induce female development. Sxl is transiently expressed in PGCs during their migration to the gonads; this expression, which was detected only in XX PGCs, is necessary for PGCs to assume a female fate. Ectopic expression of Sxl in XY PGCs was sufficient to induce them to enter oogenesis and produce functional eggs when transplanted into an XX host. Our data provide powerful evidence that Sxl initiates female germline fate during sexual development. 相似文献
10.
The recent discovery of mammalian bitter, sweet, and umami taste receptors indicates how the different taste qualities are encoded at the periphery. However, taste representations in the brain remain elusive. We used a genetic approach to visualize the neuronal circuitries of bitter and sweet tastes in mice to gain insight into how taste recognition is accomplished in the brain. By selectively expressing a transsynaptic tracer in either bitter- or sweet and/or umami-responsive taste receptor cells, and by comparing the locations of the tracer-labeled neurons in the brain, our data revealed the potential neuronal bases that underlie discrimination of bitter versus sweet. 相似文献