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排序方式: 共有314条查询结果,搜索用时 15 毫秒
1.
Kazuya KUSHIDA Urs GIGER Toshihiko TSUTSUI Megumi INABA Yoshio KONNO Kureha HAYASHI Kana NOGUCHI Akira YABUKI Keijiro MIZUKAMI Moeko KOHYAMA Yasuyuki ENDO Osamu YAMATO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(6):743-746
Erythrocyte pyruvate kinase (PK) deficiency is an inherited glycolytic erythroenzymopathy
caused by mutations of the PKLR gene. A causative mutation of the feline
PKLR gene was originally identified in Abyssinian and Somali cats in
the U.S.A. In the present study, a TaqMan probe-based real-time PCR genotyping assay was
developed and evaluated for rapid genotyping and large-scale screening for this mutation.
Furthermore, a genotyping survey was carried out in a population of four popular purebred
cats in Japan to determine the current mutant allele frequency. The assay clearly
displayed all genotypes of feline PK deficiency, indicating its suitability for
large-scale survey as well as diagnosis. The survey demonstrated that the mutant allele
frequency in Abyssinian and Somali cats was high enough to warrant measures to control and
prevent the disease. The mutant allele frequency was relatively low in Bengal and American
Shorthair cats; however, the testing should still be carried out to prevent the spread of
the disease. In addition, PK deficiency should always be considered in the differential
diagnosis of anemia in purebred cats in Japan as well as worldwide. 相似文献
2.
Viskam WIJEWARDANA Kikuya SUGIURA Daluthgamage Patsy H. WIJESEKERA Shingo HATOYA Toshiya NISHIMURA Ryoji KANEGI Takahiro USHIGUSA Toshio INABA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(7):771-775
Previously, we reported that ovarian hormones affect the immune response against
E. coli isolated from the dogs affected with pyometra. In order to
investigate mechanisms underlying the immune modulation, we examined the effects of
ovarian hormones on the generation of dendritic cells (DCs), the most potent antigen
presenting cell. DCs were differentiated from peripheral blood monocytes (PBMOs) using a
cytokine cocktail. Both estrogen receptor and progesterone receptors were expressed by the
PBMOs and immature DCs. When various ovarian hormones were added to the culture for the DC
differentiation, progesterone significantly decreased the expression of DC maturation
markers, such as CD1a, CD80 and CD86, on mature DCs. Conversely, the addition of estrogen
to the cultures increased the expression of CD86, but not other maturation makers.
Furthermore, DCs differentiated in the presence of progesterone did not stimulate
allogeneic mononuclear cells in PB. Taken together, these results indicate that
progesterone diminishes the maturation of DCs, leading to decreased immune responses
against invading pathogens. 相似文献
3.
Yasushi Ohmachi Tomomi Imamura Mizuyo Ikeda Eriko Shishikura Eunjoo Kim Osamu Kurihara Kazuo Sakai 《Journal of toxicologic pathology》2015,28(2):65-71
To evaluate the effectiveness of sodium bicarbonate (SB) in removing uranium and protecting animals from uranium toxicity, we intramuscularly administered 1 mg/kg of uranyl nitrate to 8-wk-old male SD rats, and 20 min after administration of uranyl nitrate, the animals were given a single oral administration of SB at 0.1, 0.3 or 1 g/kg. The SB treatment at a dose of 0.3 g/kg or more raised the pH of the rats’ urine until 4 h after treatment, and it significantly reduced the uranium amounts in the kidneys at 1 day after treatment. In another experiment, rats were intramuscularly administered 1 mg/kg of uranyl nitrate, and 20 min later, the animals were treated with sodium bicarbonate (0.1 or 1 g/kg). The rats were autopsied at 1, 3 and 7 days after uranium treatment. High-dose SB resulted in a significant increase in urinary uranium excretion in the first 24 h and a reduction of uranium deposition in the kidneys and femurs, and it also significantly suppressed uranium-induced renal toxicity, as shown by both histopathology and clinical chemistry at 3 days after uranium treatment. Low-dose SB did not show such marked effects. Our findings demonstrated that the uranium decorporation effect of sodium bicarbonate was observed at the dosage showing urine alkalinization in rats and that decorporation effect of sodium bicarbonate might be beneficial if it is administered immediately after incorporation of soluble uranium. 相似文献
4.
To clarify mechanisms underlying variation in transpiration rate among deciduous broad-leaved tree species, we measured diurnal changes in stomatal conductance (gs) and leaf water potential, and calculated the maximum transpiration rate (Emax), leaf-specific hydraulic conductance (K(s-l)) and difference between the soil water potential and the daily minimum leaf water potential (Psis - Psi(l,min)). Pressure-volume (P-V) measurements were made on leaves. Saplings of eight broad-leaved tree species that are common in Japanese cool temperate forests were studied. Maximum transpiration rate varied significantly among species. There was a statistically significant difference in Psis - Psi(l,min), but not in K(s-l). Species with large Emax also had large Psis - Psi(l,min) and gs. The results of the P-V analyses showed that species with a large Psis - Psi(l,min) maintained turgor even at low leaf water potentials. The similar daily minimum leaf pressure potentials (Psip) across all eight species indicate that Psip values below this minimum are critical. Based on these results, we suggest that the leaf cell capacity for turgor maintenance strongly affects Psis - Psi(l,min) and consequently Emax via stomatal regulation. 相似文献
5.
Kraft pulp bleaching effluent supplemented with glucose was decolorized by a newly isolated fungusGeotrichum candidum Dec 1 (Dec 1) that showed a wide decolorizing spectrum to synthetic dyes. When the glucose concentration in the effluent was 30 g/l, the color removal and the reduction of absorbable organic halogens were 78% and 43% after 7 days culture, respectively. The average molecular weight of colored substances measured by gel filtration chromatography was lowered to less than 3000 from 5600 after 7 days culture. As the contribution of extracellular enzymes such as peroxidase (DyP), manganese peroxidase, and laccase to the decolorization of the kraft pulp bleaching effluent was small, Dec 1 appears to have a different mechanism of decolorizing kraft pulp bleaching effluent when compared with enzymes used to decolorize synthetic dyes. 相似文献
6.
Yasushi?HiramatsuEmail author Kiyohiko?Fujimoto Atsushi?Miyatake Kenta?Shindo Hirofumi?Nagao Hideo?Kato Hirofumi?Ido 《Journal of Wood Science》2011,57(1):66-70
The purpose of this study was to investigate the strength properties of glued laminated timber composed of edge-glued laminae
and to investigate the influence of edge gluing on the strength properties. Glued laminated timber composed of multiple-grade
laminae (symmetrical composition, strength grade E95-F270, 10 laminations) was produced from karamatsu (Larix kaempferi) edge-glued laminae according to the Japanese Agricultural Standard. The bending, tensile, and compressive strengths of the
glued laminated timber were measured. The average bending, tensile, and compressive strengths were 33.4, 24.5, and 35.9 MPa,
respectively, and these values are almost equal to those of glued laminated timber composed of karamatsu single-piece laminae.
It was determined that finger-joints and knots in the edge-glued laminae played a significant role in the failure of specimens.
However, the use of glued edge-joints did not appear to affect the failure of specimens. 相似文献
7.
In order to develop a fireproof wooden material, the synergic effect of fire-retardant chemicals and wood coatings was studied.
The fire performance was evaluated by cone calorimeter. Impregnation of fire retardants including polyphosphatic carbamate,
and ceramic coatings including alkoxy metal salt improved the fire performance of wooden materials. This treatment made it
possible to meet the guidelines for fire performance of noncombustible materials in Japan. In addition to the vacuum-pressure
impregnation treatment, hot-and-cold-bath impregnation treatment is an effective way to develop fire-retardant wood by impregnating
fire retardant and ceramic coating. The weatherability of the developed material was also investigated. The ceramic coating
was resistant to light and moisture.
Part of this report was presented at 54th Annual Meeting (Sapporo, August 2004) and the 55th Annual Meeting (Kyoto, March
2005) of the Japan Wood Research Society 相似文献
8.
Yi Min Wei Bunichiro Tomita Yasushi Hiramatsu Atsushi Miyatake Tsuyoshi Fujii Tomoyuki Fujii Shuichiro Yoshinaga 《Journal of Wood Science》2003,49(4):317-326
Poor compatibility was found between exploded wood fiber strand (WFS) and cement due to the excessive presence of water-soluble degraded polysaccharides in extractives of exploded WFS obtained from weathered wood waste treated by the water-vapor explosion process (WVEP). This study presents some comparative results from a continuing investigation on the compressive strengths of exploded WFS–cement mixtures. Based on results previously obtained with the hydration test, the relation between hydration behavior and compressive strength of the mixture was explored. In addition, the effect of the curing age on compressive strength development of the mixture with selected additive chemicals was examined. The results supported the results of early studies with hydration tests indicating that adding MgCl2 to the mixtures of exploded WFS mixed with quick-curing cement or ordinary Portland cement and a composite of MgCl2 + CaO added to the mixture of exploded WFS and furnace-slag cement effectively improved the hydration behaviors; it greatly enhanced the compressive strengths of mixtures as well. Compressive strengths were strongly correlated to maximum hydration temperatures (Tmax) of wood–cement mixtures influenced by the cement type, wood wastes (treated or not with WVEP), additive chemicals, and their content levels. The results also indicated that adding selected chemicals had no significant effect on compressive strength among the mixtures of exploded WFS mixed, respectively, with three types of cement at a curing age of 180 days. X-ray diffraction, scanning electron microscopy, and energy dispersive X-ray spectroscopy were used to identify the hydration products and to probe the element distribution of the mixture in the wood–cement interface zone from a fractured surface.Part of this report was presented at the 52nd Annual Meeting of the Japan Wood Research Society, Gifu, April 2002 相似文献
9.
Ken‐ichi YAMANAKA Masahiro KANEDA Yasushi INABA Koji SAITO Kaiyu KUBOTA Miki SAKATANI Satoshi SUGIMURA Kei IMAI Shinya WATANABE Masashi TAKAHASHI 《Animal Science Journal》2011,82(4):523-530
Many observations have been made on cloned embryos and on adult clones by somatic cell nuclear transfer (SCNT), but it is still unclear whether the progeny of cloned animals is presenting normal epigenetic status. Here, in order to accumulate the information for evaluating the normality of cloned cattle, we analyzed the DNA methylation status on satellite I region in blastocysts obtained from cloned cattle. Embryos were produced by artificial insemination (AI) to non‐cloned or cloned dams using semen from non‐cloned or cloned sires. After 7 days of AI, embryos at blastocyst stage were collected by uterine flushing. The DNA methylation levels in embryos obtained by using semen and/or oocytes from cloned cattle were similar to those in in vivo embryos from non‐cloned cattle. In contrast, the DNA methylation levels in SCNT embryos were significantly higher (P < 0.01) than those in in vivo embryos from non‐cloned and cloned cattle, approximately similar to those in somatic cells used as donor cells. Thus, this study provides useful information that epigenetic status may be normal in the progeny of cloned cattle, suggesting the normality of germline cells in cloned cattle. 相似文献
10.
Li CP Ibrahim HR Sugimoto Y Hatta H Aoki T 《Journal of agricultural and food chemistry》2004,52(18):5752-5758
Egg white protein (EWP) was phosphorylated by dry-heating in the presence of pyrophosphate at pH 3.0-7.0 and 85 degrees C for 1 and 5 days, and the functional properties of the phosphorylated EWP (PP-EWP) were investigated. The phosphorylation was accelerated with a decrease of pH from 7.0 to 3.0 and for heating times from 1 to 5 days. The phosphorus content of EWP increased approximately 1.05% by dry-heating at pH 4.0 and 85 degrees C for 5 days in the presence of pyrophosphate, which was higher than that of casein. The electrophoretic mobility of EWP increased with an increase in the phosphorylation level. The surface hydrophobicity of EWP increased by phosphorylation. The heat stability, emulsifying properties, and digestibility of EWP were improved by phosphorylation. The calcium phosphate-solubilizing ability of EWP was enhanced by phosphorylation. A firmer and transparent heat-induced gel of PP-EWP was obtained, and the water-holding capacity of heat-induced PP-EWP gel was higher that that of the control. These results suggest that phosphorylation by dry-heating in the presence of pyrophosphate is a useful method for improving the functional properties of EWP. 相似文献