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排序方式: 共有228条查询结果,搜索用时 31 毫秒
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Masato HIYAMA Ken Takeshi KUSAKABE Ai TAKESHITA Sinji SUGI Nobue KUNIYOSHI Hiroyuki IMAI Kiyoshi KANO Yasuo KISO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(3):305-311
LC3 − the mammalian homolog of Atg8 − was found as autophagosome membrane binding protein
in mammals and widely used as an autophagosomal marker. LC3A, B and C show different
expression patterns in each tissue. The aim of this study was to reveal the differences of
expression patterns among LC3 families in mouse placenta under normal condition and
nutrient starving condition. LC3A and B were highly expressed in decidual cells. LC3A and
B were increased in D14 compared with D12 and D16 in mouse placenta, while LC3C was
decreased. Starvation induced increase in LC3B expression specifically.
Immunohistochemistry showed different expression patterns among LC3A, B and C. LC3A
expression in syncytiotrophoblast was vanished by starvation. The results of real time
RT-PCR suggested differences between D12 and D16 in autophagic cascade induced by
starvation. Taken together, this study suggests that autophagy could play a role in
placental invasion system and that nutrient starvation affects LC3B expression. 相似文献
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Ken‐ichi YAMANAKA Masahiro KANEDA Yasushi INABA Koji SAITO Kaiyu KUBOTA Miki SAKATANI Satoshi SUGIMURA Kei IMAI Shinya WATANABE Masashi TAKAHASHI 《Animal Science Journal》2011,82(4):523-530
Many observations have been made on cloned embryos and on adult clones by somatic cell nuclear transfer (SCNT), but it is still unclear whether the progeny of cloned animals is presenting normal epigenetic status. Here, in order to accumulate the information for evaluating the normality of cloned cattle, we analyzed the DNA methylation status on satellite I region in blastocysts obtained from cloned cattle. Embryos were produced by artificial insemination (AI) to non‐cloned or cloned dams using semen from non‐cloned or cloned sires. After 7 days of AI, embryos at blastocyst stage were collected by uterine flushing. The DNA methylation levels in embryos obtained by using semen and/or oocytes from cloned cattle were similar to those in in vivo embryos from non‐cloned cattle. In contrast, the DNA methylation levels in SCNT embryos were significantly higher (P < 0.01) than those in in vivo embryos from non‐cloned and cloned cattle, approximately similar to those in somatic cells used as donor cells. Thus, this study provides useful information that epigenetic status may be normal in the progeny of cloned cattle, suggesting the normality of germline cells in cloned cattle. 相似文献
5.
To evaluate windthrow resistance with respect to stem breakage, a nondestructive method for determining the shape of trunk
cross sections was developed. In this method, the coordinates of multiple gauge points set on the perimeter of a trunk are
calculated by measuring the distances between them. The shape between the gauge points is generated with the use of a profile
gauge placed between them. Measurement tests were conducted using profile gauges with lengths of 300 and 900 mm on model specimens
with four shape patterns and four different diameters. The accuracy of the estimation was verified by comparing the section
modulus calculated for the generated image and for the photograph. The average ratio of section modulus (generated/photo)
for all specimens was 0.994, which indicates that the proposed method is highly accurate. The section moduli of hollow trunks
can be evaluated using the profile method together with the drill resistance technique on the condition that 26% of the trunk
diameter could be drilled without skew. 相似文献
6.
Yasushi Narita Kei Satoh Keiichi Hayashi Tamami Iwase Shigeru Tanaka Yukiko Dokiya Morikazu Hosoe Kazuhiko Hayashi 《Water, air, and soil pollution》2001,130(1-4):1649-1654
In recent years, acid rain has been a social problem all over the world. In Japan, it is also a big problem especially in the metropolitan area. Then, we have measured major ions such as H+, Na+, NH4 +, K+, Mg2+, Ca2+, Cl?, NO3 ?, and SO4 2? in precipitation and dry deposition samples which had been collected at 9 sampling sites at Hiyoshi, Mita, Kashiwa, Shiki, Fujisawa, Yokosuka, Mitaka, Hachiouji, and Ashikaga in Tokyo Metropolitan area for 10 years since 1990. The average pH of precipitation in their sites was 4.56 (n=1906). As the results of multiple regression analysis showed that pH of precipitation was determined by 5 ions such as NH4 +, nssCa2+(non sea salt calcium), nssCl?(non sea salt chloride), NO3 ?, nssSO4 2?(non sea salt sulfate) in the most of the sampling sites. Therefore, it is very important to investigate the behavior of these ions to understand the acidification of rain in Tokyo Metropolitan area. In this study, a long term trend of each ion concentration in precipitation and wet deposition was also investigated the base on the data we had observed at 7 sites for 10 years by the statistical method. 相似文献
7.
Peng WANG Kousaku SOUMA Yuki KOBAYASHI Kei IWABUCHI Chihiro SATO Takayoshi MASUKO 《Animal Science Journal》2010,81(4):487-493
This study examined the differences between non‐inoculated (control) corn and Northern Leaf Blight (NLB)‐damaged corn (inoculated corn); dry matter (DM) yield, silage fermentation quality, nutritive value and feed intake by sheep were compared. Leaf, stem and grain dry weights and gross yield of inoculated corn were significantly (P < 0.05) decreased compared with control corn. The contents of water‐soluble carbohydrate and nitrogen‐free extract (NFE) were decreased in inoculated corn compared with control corn. Silage made from both inoculated and control corn showed good fermentation quality. The digestibility of DM, organic matter, ether extract, NFE, and energy of silage made from inoculated corn were significantly (P < 0.05) lower, and contents of total digestible nutrients (TDN) and digestible energy (DE) were also significantly (P < 0.05) lower compared with silage made from the control corn. DM intake showed no significant discrepancy between the two types of silage. TDN and DE intakes from inoculated silage were significantly (P < 0.05) lower compared with control silage. From the above results it was shown that NLB caused a decrease in DM yield and NFE content in corn and a decrease in the nutritive value and feed intake of silage. 相似文献
8.
Asami Nakanishi Yoshiyuki Inagaki Naoya Osawa Shozo Shibata Kei’ichi Hirata 《Journal of Forest Research》2009,14(6):388-393
Leaf nitrogen nutrition of hinoki cypress (Chamaecyparis obtusa Endlicher) was investigated at three positions along a slope over a period of 3 years. At each slope position, nitrogen properties
were compared in patch-cut plots (0.06–0.09 ha) and uncut control plots (0.04 ha). Nitrogen cycling at the lower slope was
characterized by a higher rate of soil nitrogen mineralization, and higher nitrogen concentration in fresh leaves and leaf-litter.
The soil nitrogen mineralization rate and fresh-leaf nitrogen concentration in the patch-cut plots were higher than those
in the control plots. However, leaf-litter nitrogen concentration did not differ between the patch-cut and control plots.
The results suggest that slope position strongly affects leaf nitrogen nutrition of hinoki cypress and soil nitrogen availability.
By contrast, patch cutting does not affect leaf-litter nitrogen concentration. These findings indicated that hinoki cypress
would not enhance forest nitrogen cycling through changes in leaf-litter nitrogen concentration after patch cutting. 相似文献
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Effect of increased feeding of dietary α‐linolenic acid by grazing on formation of the cis9,trans11–18:2 isoform of conjugated linoleic acid in bovine milk 下载免费PDF全文
Mio Yamaguchi Taki Naito Yoshikazu Nagao Yukihito Kabuyama Kei Hashimoto Norihiro Azuma Isamu Maeda 《Animal Science Journal》2017,88(7):1006-1011
Feeding systems such as grazing affect the fatty acid profile of bovine milk fat. In addition, milk fat is formed as the product of fatty acid metabolism in cow bodies before being secreted into milk. However, how grazing influences milk fatty acid profile through the metabolism has not been completely characterized. When fatty acid concentrations in Holstein milk were compared between grazing and non‐grazing periods, α‐linolenic acid was significantly higher in the grazing period than in the non‐grazing period. This could be explained with an increase in α‐linolenic acid feeding with grazing. α‐linolenic acid had a linear positive correlation with conjugated linoleic acid (9c,11t‐18:2) (CLA) and vaccenic acid (VA) during the grazing period, whereas CLA had higher correlation with linoleic acid rather than with α‐linolenic acid during the non‐grazing period. These data indicate that the high content of dietary α‐linolenic acid affects CLA and VA formation in milk of grazing periods via α‐linolenic acid metabolism into VA. 相似文献