Study on Expression Difference of IGFBP-5 Gene in Different Tissues of Kazakh and Yanqi Horses

The study aimed to explore the mRNA expression pattern of insulin-like growth factor binding protein-5 (IGFBP-5) gene in different tissues of Kazakh and Yanqi horses.The expression of IGFBP-5 gene in different tissues of heart,liver,spleen,lung,kidney,small intestine,large intestine,cecum,intercostal muscles,longissimus dorsi muscles,brachialis muscle and gluteus in two horses were detected by Real-time quantitative PCR and compared the mRNA expression in the same tissues of two breeds.The results showed that the expression of IGFBP-5 in longissimus dorsi muscle and brachialis muscle of two breeds were significantly higher than other tissues including heart,liver,spleen,lung,kidney,small intestine,large intestine and cecum (P<0.05),and was the lowest in large intestine.The expression of IGFBP-5 in kidney,small intestine,large intestine,cecum,longissimus dorsi muscle and brachialis muscles of Yanqi horse were higher than in the same part of Kazakh horse,and among those in longissimus dorsi muscle and large intestine of Yanqi horse were extremely significantly higher than in the same part of Kazakh horse (P<0.01),and in small intestine and cecum of Yanqi horse were significantly higher than in the same part of Kazakh horse (P<0.05).The test was for further researching the biological function of IGFBP-5 gene,and it could provide a theoretical basis for genetic improvement of production performance of horse in our country.     

间歇训练对青年伊犁马血液生化指标的影响

【目的】通过对进行间歇训练的6匹青年伊犁马训练前、后12 km测试赛各阶段血液指标变化规律的分析,探究间歇训练对其血液生化指标变化规律的影响,为耐力马训练体系的建立和培育优质国产耐力马提供数据支持。【方法】分别于训练前后测试赛赛前、赛后即刻、赛后15 min、赛后90 min检测血浆中谷丙转氨酶(ALT)、谷草转氨酶(AST)、肌酸激酶(CK)、乳酸脱氢酶(LDH)、血尿素(UREA)和乳酸(LAC)6项血液生化指标,对其进行方差分析,并对比训练前后的变化规律。【结果】青年伊犁马通过9周间歇训练后,12 km测试赛用时极显著低于训练前(P0.01)。训练前后马匹测试赛血浆CK、LDH变化趋势明显,训练前血浆CK、LDH赛后即刻升高,随后下降,再上升,赛后90 min CK极显著高于赛前(P0.01),LDH显著高于赛前(P0.05);训练后血浆CK、LDH均表现出现先上升,后下降,赛后90 min极显著高于赛前(P0.01)。训练前后血浆ALT、AST、UREA、LAC与训练前变化趋势基本一致,但变化幅度较训练前更大,最大LAC较训练前提高了33.2%。【结论】马匹经9周间歇训练后,训练后测试赛马匹骨骼肌运动损伤小,具有更高训练程度,机体对血浆ALT、AST、LAC的耐受性有所提高。     

哈萨克马和焉耆马不同组织中IGFBP-5基因表达差异研究

试验旨在探求胰岛素样生长因子结合蛋白-5（insulin-like growth factor binding protein-5,IGFBP-5）基因在哈萨克马和焉耆马不同组织部位中的mRNA表达规律。采用实时荧光定量PCR技术检测在哈萨克马和焉耆马心脏、肝脏、脾脏、肺脏、肾脏、小肠、大肠、盲肠、肋间肌、背最长肌、臂肌和臀肌等不同组织中IGFBP-5基因mRNA表达量,同时比较该基因在哈萨克马和焉耆马相同组织中的表达差异。结果表明,哈萨克马和焉耆马表达量最高的均为背最长肌,其次是臂肌,并显著高于心脏、肝脏、脾脏、肺脏、肾脏、小肠、大肠、盲肠等内脏组织表达量（P<0.05）,大肠中的表达量最低;焉耆马的肾脏、小肠、大肠、盲肠、背最长肌和臂肌中表达量高于哈萨克马相同部位的表达量,其中焉耆马背最长肌和大肠中的表达量极显著高于哈萨克马的相同部位（P<0.01）,小肠和盲肠中的表达量显著高于哈萨克马的相同部位（P<0.05）。本试验为深入研究IGFBP-5基因的生物学功能,以及对中国马生产性能的遗传改良提供理论依据。     

伊犁马青年种公马体尺体重相关性研究

为探讨伊犁马青年种公马体尺、体重之间的相关性,选择2～3岁肉用型和骑乘型伊犁马青年种公马100匹,测量其体高、体长、胸围、管围和体重数据,并进行统计分析.结果表明,伊犁马青年种公马不同类型不同年龄体尺、体重之间存在显著差异(P<0.05).其体高、体长、体重均以3岁肉用型为最高,其次为3岁骑乘型(P<0.05).其胸围...     

不同用途马肢体各部位比例差异性分析研究

本试验通过测定纯血马×伊犁马、奥尔洛夫马×伊犁马、俄罗斯速步马×伊犁马和汉诺威马4个类型马细分体尺,并计算各体尺比例及体尺指数等指标,分析各体尺、体尺指数及各比例差异性。结果表明,在体高、体长、胸围、管围、颈长、肱骨长、背长、腰椎长、尻长、尻高、股骨长和胫骨长等体尺方面,纯血马×伊犁马、奥尔洛夫马×伊犁马、俄罗斯速步马×伊犁马均极显著低于汉诺威马（P<0.01）;体躯指数方面,纯血马×伊犁马显著高于奥尔洛夫马×伊犁马（P<0.05）;肱骨长/背长、股骨长/背长和胫骨长/背长方面,纯血马×伊犁马、奥尔洛夫马×伊犁马、俄罗斯速步马×伊犁马均极显著低于汉诺威马（P<0.01）;奥尔洛夫马×伊犁马和俄罗斯速步马×伊犁马之间所有指标差异均不显著（P>0.05）。本试验结果表明不同用途马匹体型结构具有一定差异性,为不同用途马匹的培育提供数据参考。     

Difference Analysis of Body Proportion in Different Uses Horse Breeds

Measuring subsection body measurements of Thoroughbred×Yili horses,Orlov trotter×Yili horses,Russia trotter×Yili horses and Hanoverian horse,and calculated the proportion and index,analysing differences in each body measurement,index and proportion.The results showed that the body height,body length,chest circumference,cannon bone circumference,neck length,humerus length,back length,lumbar spine,rump length,rump high,thighbone length and tibiae length of Thoroughbred×Yili horses,Orlov trotter×Yili horses and Russia trotter×Yili horses were extremely significantly lower than Hanoverian horse (P<0.01),the trunk index of Thoroughbred×Yili horse was significantly higher than Orlov trotter×Yili horses (P<0.05),the humerus length/back length,thighbone length/back length and tibiae length/back length of Thoroughbred×Yili horses,Orlov trotter×Yili horses and Russia trotter×Yili horses were extremely significantly lower than Hanoverian horse (P<0.01),all body measurements of Orlov trotter×Yili horses had no significant difference with Russia trotter×Yili horses (P>0.05).The experiment results showed that the difference in body structure of horses for different uses could provide data reference for breeding these horses.