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排序方式: 共有133条查询结果,搜索用时 15 毫秒
1.
Kazumi KISHIDA Mitsuhiro SAKASE Kenta MINAMI Miyuki M. ARAI Reiko SYOJI Namiko KOHAMA Takayuki AKIYAMA Akio OKA Hiroshi HARAYAMA Moriyuki FUKUSHIMA 《The Journal of reproduction and development》2015,61(6):519-524
The purposes of this study were to examine the relationship between male artificial insemination (AI)
fertility and sperm acrosomal conditions assessed by new and conventional staining techniques and to identify
possible reproductive dysfunctions causing low conception rates in AI using frozen-thawed spermatozoa with
poor acrosomal conditions in Japanese Black bulls. We investigated individual differences among bulls in the
results concerning (1) acrosomal conditions of frozen-thawed spermatozoa as assessed by not merely peanut
agglutinin-lectin staining (a conventional staining technique) but also immunostaining of acrosomal
tyrosine-phosphorylated proteins (a new staining technique), (2) routine AI using frozen-thawed spermatozoa as
assessed by pregnancy diagnosis, (3) in vivo fertilization of frozen-thawed spermatozoa and
early development of fertilized eggs as assessed by superovulation/AI-embryo collection tests and (4)
in vitro fertilization of frozen-thawed spermatozoa with oocytes. The percentages of
frozen-thawed spermatozoa with normal acrosomal conditions assessed by the abovementioned staining techniques
were significantly correlated with the conception rates of routine AI, rates of transferable embryos in
superovulation/AI-embryo collection tests and in vitro fertilization rates. These results are
consistent with new suggestions that the distribution of acrosomal tyrosine-phosphorylated proteins as well as
the acrosomal morphology of frozen-thawed spermatozoa are AI fertility-associated markers that are valid for
the prediction of AI results and that low conception rates in AI using frozen-thawed spermatozoa with poor
acrosomal conditions result from reproductive dysfunctions in the processes between sperm insemination into
females and early embryo development, probably failed fertilization of frozen-thawed spermatozoa with
oocytes. 相似文献
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4.
Multifunctionality and agrarian transition in alternative agro‐food production in the global South: The case of organic shrimp certification in the Mekong Delta,Vietnam 下载免费PDF全文
Processes of globalisation in the conventional food provision system have had widespread negative impacts on small‐scale farmers. Yet, alternative food networks, which are characterised by more sustainable production/consumption practices and fairer trade relations, have increasingly been ‘going global’ and, in the process, have been integrating small‐scale farms in the South. One such high‐value export‐led commodity is certified organic shrimp. International third‐party certification schemes are becoming popular as a tool to verify the intangible attributes of such commodities. Using concepts of multifunctionality and agrarian change, this paper examines the implications of introducing an international environmental certification programme to a site where the ‘peasantry’ has been preserved and has limited integration in the global agro‐food system. Drawing on a case study that examines the first certified organic shrimp production project in Vietnam, this paper concludes that the current movement towards post‐productivism in the global North has potential to keep local farming practices in the global South by justifying the value of peasant‐like production methods through international certification. As a result, the development path of agrarian transition might be reshaped into a form not necessarily pursuing industrialisation. This leads to the new interpretation of pre‐ and post‐productivism beyond the North and South divide. 相似文献
5.
Hironori Koga Koji Dohi Reiko Yoshimoto Masashi Mori 《Journal of General Plant Pathology》2008,74(3):246-249
Resistance in the leaf blades of rice plants against a virulent race of the rice blast fungus Magnaporthe oryzae was quantitatively examined using a modified spot inoculation method. Numbers of conidia produced in the lesions were affected
by plant age and paralleled the frequency of resistance infection types, which is indicative of whole-plant-specific resistance
(WPSR), in the inoculated leaf sheaths of the corresponding plants. Exogenous abscisic acid treatment, which suppresses WPSR,
also increased the susceptibility of the leaf blades. These results indicate a correlation between the resistance of the leaf
blades and the WPSR in the leaf sheaths. 相似文献
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Pseurotins A1 (1) and A2 (2) were isolated from a culture broth of the fungal strain Aspergillus fumigatus WFZ-25 as stereoisomers of pseurotin A (3) in 2011. We also isolated 1 and 2 together with 3 from A. fumigatus OUPS-T106B-5 separated from the marine fish Mugil cephalus. In this study, we re-examined the stereochemistry of 1 and 2 using chemical transformation and the CD spectra, and found the relationship between the CD Cotton effect and the absolute configurations of 1 and 2, which led us to revise the stereostructure of pseurotin A2. 相似文献
8.
Chhourn Orn Rieko Shishido Masahiro Akimoto Ryo Ishikawa Than Myint Htun Ken-Ichi Nonomura Yohei Koide Men Sarom Seng Vang Sakhan Sophany Ouk Makara Takashige Ishii 《Breeding Science》2015,65(5):430-437
A total of 448 samples in five natural populations of wild rice (Oryza rufipogon) were collected in Cambodia. They were examined using 12 SSR and two chloroplast markers to evaluate the degree of variation among populations and the genetic structure within populations. In the two annual populations, the number of plants with homozygous alleles at all 12 SSR loci were high (66.3% and 79.5%), suggesting that these plants propagate mainly through self-pollination. In the three perennial populations, no individuals had all homozygous genotypes, but redundant genotypes resulted from clonal propagation were observed. Percentages of the redundant genotypes were highly varied (3.6%, 29.2% and 86.0%). This may be due to the different stable levels of environmental conditions. As for chloroplast genome, most of the wild plants showed the same chloroplast types as most Indica-type cultivars have. However, plants with different chloroplast types were maintained, even in the same population. In tropical Asian countries, many wild rice populations were observed under similar ecological conditions examined in this study. Therefore, the present results concerning population structure will be important to further elucidate genetic features of wild rice, and will also give strong clues to utilize and conserve wild natural genetic resources. 相似文献
9.
Shimizu E Kato H Nakagawa Y Kodama T Futo S Minegishi Y Watanabe T Akiyama H Teshima R Furui S Hino A Kitta K 《Journal of agricultural and food chemistry》2008,56(14):5521-5527
A novel type of quantitative competitive polymerase chain reaction (QC-PCR) system for the detection and quantification of the Roundup Ready soybean (RRS) was developed. This system was designed based on the advantage of a fully validated real-time PCR method used for the quantification of RRS in Japan. A plasmid was constructed as a competitor plasmid for the detection and quantification of genetically modified soy, RRS. The plasmid contained the construct-specific sequence of RRS and the taxon-specific sequence of lectin1 (Le1), and both had 21 bp oligonucleotide insertion in the sequences. The plasmid DNA was used as a reference molecule instead of ground seeds, which enabled us to precisely and stably adjust the copy number of targets. The present study demonstrated that the novel plasmid-based QC-PCR method could be a simple and feasible alternative to the real-time PCR method used for the quantification of genetically modified organism contents. 相似文献
10.
R. Shishido M. Kikuchi K. Nomura H. Ikehashi 《Genetic Resources and Crop Evolution》2006,53(1):179-186
As a part of an in situ survey of wild rice (Oryza rufipogon Griff.) in Myanmar (Burma), 16 strains of wild rice were collected, and analyzed for allelic diversity over 74 loci with
simple sequence repeat (SSR) markers to obtain a basic information for their conservation. Three each of indica and japonica cultivars were added for a comparison. In the six cultivars and 16 strains of wild rice, three to 15 alleles were detected
per locus with an average of 7.9. The wild rice revealed a large number of unique alleles throughout their chromosomes with
much wider ranges of variation than those detected in the six cultivars of O. sativa L.. The alleles found in the wild rice were classified into those specific to wild rice, common to wild rice and cultivars,
and those similar to indica or japonica cultivars. According to the classification, the genotype of each of the 16 strains of wild rice was schematically depicted.
The genetic variation among individual strains within a collection site was larger than the variation among the collection
sites. 相似文献