全文获取类型
收费全文 | 119篇 |
免费 | 8篇 |
专业分类
林业 | 9篇 |
农学 | 1篇 |
26篇 | |
综合类 | 6篇 |
农作物 | 4篇 |
水产渔业 | 18篇 |
畜牧兽医 | 36篇 |
植物保护 | 27篇 |
出版年
2023年 | 2篇 |
2022年 | 1篇 |
2021年 | 5篇 |
2020年 | 7篇 |
2019年 | 5篇 |
2018年 | 3篇 |
2017年 | 2篇 |
2016年 | 7篇 |
2015年 | 5篇 |
2014年 | 6篇 |
2013年 | 12篇 |
2012年 | 6篇 |
2011年 | 8篇 |
2010年 | 8篇 |
2009年 | 6篇 |
2008年 | 9篇 |
2007年 | 2篇 |
2006年 | 5篇 |
2005年 | 4篇 |
2004年 | 8篇 |
2003年 | 5篇 |
2002年 | 5篇 |
2001年 | 1篇 |
2000年 | 1篇 |
1996年 | 1篇 |
1990年 | 1篇 |
1989年 | 1篇 |
1975年 | 1篇 |
排序方式: 共有127条查询结果,搜索用时 46 毫秒
1.
2.
Kazumi KISHIDA Mitsuhiro SAKASE Kenta MINAMI Miyuki M. ARAI Reiko SYOJI Namiko KOHAMA Takayuki AKIYAMA Akio OKA Hiroshi HARAYAMA Moriyuki FUKUSHIMA 《The Journal of reproduction and development》2015,61(6):519-524
The purposes of this study were to examine the relationship between male artificial insemination (AI)
fertility and sperm acrosomal conditions assessed by new and conventional staining techniques and to identify
possible reproductive dysfunctions causing low conception rates in AI using frozen-thawed spermatozoa with
poor acrosomal conditions in Japanese Black bulls. We investigated individual differences among bulls in the
results concerning (1) acrosomal conditions of frozen-thawed spermatozoa as assessed by not merely peanut
agglutinin-lectin staining (a conventional staining technique) but also immunostaining of acrosomal
tyrosine-phosphorylated proteins (a new staining technique), (2) routine AI using frozen-thawed spermatozoa as
assessed by pregnancy diagnosis, (3) in vivo fertilization of frozen-thawed spermatozoa and
early development of fertilized eggs as assessed by superovulation/AI-embryo collection tests and (4)
in vitro fertilization of frozen-thawed spermatozoa with oocytes. The percentages of
frozen-thawed spermatozoa with normal acrosomal conditions assessed by the abovementioned staining techniques
were significantly correlated with the conception rates of routine AI, rates of transferable embryos in
superovulation/AI-embryo collection tests and in vitro fertilization rates. These results are
consistent with new suggestions that the distribution of acrosomal tyrosine-phosphorylated proteins as well as
the acrosomal morphology of frozen-thawed spermatozoa are AI fertility-associated markers that are valid for
the prediction of AI results and that low conception rates in AI using frozen-thawed spermatozoa with poor
acrosomal conditions result from reproductive dysfunctions in the processes between sperm insemination into
females and early embryo development, probably failed fertilization of frozen-thawed spermatozoa with
oocytes. 相似文献
3.
4.
Shimizu E Kato H Nakagawa Y Kodama T Futo S Minegishi Y Watanabe T Akiyama H Teshima R Furui S Hino A Kitta K 《Journal of agricultural and food chemistry》2008,56(14):5521-5527
A novel type of quantitative competitive polymerase chain reaction (QC-PCR) system for the detection and quantification of the Roundup Ready soybean (RRS) was developed. This system was designed based on the advantage of a fully validated real-time PCR method used for the quantification of RRS in Japan. A plasmid was constructed as a competitor plasmid for the detection and quantification of genetically modified soy, RRS. The plasmid contained the construct-specific sequence of RRS and the taxon-specific sequence of lectin1 (Le1), and both had 21 bp oligonucleotide insertion in the sequences. The plasmid DNA was used as a reference molecule instead of ground seeds, which enabled us to precisely and stably adjust the copy number of targets. The present study demonstrated that the novel plasmid-based QC-PCR method could be a simple and feasible alternative to the real-time PCR method used for the quantification of genetically modified organism contents. 相似文献
5.
Azusa SOMEYA Ryoko FUKUSHIMA Michiko YOSHIDA Yasuyuki TANAHASHI Tangmunkhong PRAPEUK Reiko IIZUKA Hiroshi HIRAMI Atsushi MATSUDA Shunichi TAKAHASHI Goro KURITA Takashi KIMURA Misuzu SEO Masayuki FUNABA Yoshii NISHINO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(8):1157-1160
6.
7.
8.
Sachie Ikegami Masako Tomita Setsuko Honda Momoko Yamaguchi Reiko Mizukawa Yoko Suzuki Keiko Ishii Saeko Ohsawa Nanako Kiyooka Mitsuru Higuchi Shuhei Kobayashi 《Plant foods for human nutrition (Dordrecht, Netherlands)》1996,49(4):317-328
Barley contains approximately 10% dietary fiber and is easily cooked with rice, the dominant cereal in Japan, to increase the intake of dietary fiber. This research involved three experiments to examine the influence of barley on blood lipids in human subjects. All subjects received a boiled barley-rice (50/50 w/w mix) supplement two times per day in place of rice for 2 or 4 weeks. In the normolipemic subjects, serum lipids were unaffected by the ingestion of barley for 4 weeks. In twenty hypercholesterolemic men aged 41 ± 5 years, the ingestion of barley was associated with a significant fall in serum total cholesterol, LDL-cholesterol, phospholipids and LDL and VLDL-lipoproteins. In seven mildly hypercholesterolemic women aged 56±7 years, a significant improvement of serum lipid profiles was observed. The present study suggests the possibility that the ingestion of barley-rice could lower serum lipids in hypercholesterolemic subjects. 相似文献
9.
Kenji KUTARA Sho KADEKARU Reiko SUGISAWA Yumi UNE 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(6):947
An adult female red deer died of a severe seizure and dysbasia. Postmortem computed tomography (CT) and magnetic resonance imaging (MRI) were performed. On CT, deciduous right maxillary second and third premolar teeth were observed, and the right infraorbital canal was disrupted. MRI showed that the right trigeminal nerve was enlarged and the right subarachnoid cavity was occupied by fluid and gas. On gross examination, the right paranasal sinus, swollen muscles of the orbit and tonsils, right trigeminal nerve, and right cerebrum surface contained a yellowish-white, cheese-like pus. Based on these findings, the deer was believed to have developed pyogenic meningitis caused by a neuropathic infection secondary to periodontogenic paranasal sinusitis. 相似文献
10.
Usui R Usui R Fukuda M Fukui E Hasegawa A 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(9):1249-1252
Otitis externa in 27 toy poodles and 40 miniature dachshunds was treated using a video otoscope. A distinct concavity (external tympanic concavity) was observed at the junction between the ventral part of the external surface of the tympanum and the ear canal to which a considerable amount of hair and debris had adhered. All hair and debris adhering to the external tympanic concavity were removed, and systemic antibiotic and antifungal agents were administered, after which all of the dogs recovered. The pattern of hair growth observed in the external tympanic concavity could be characterized according to the breed of dog. All of the toy poodles presented with curly hairs, while the miniature dachshunds had upright or flat-lying hairs. 相似文献