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排序方式: 共有628条查询结果,搜索用时 46 毫秒
1.
Journal of Crop Science and Biotechnology - We examined the effects of drought stress on flowering time, grain yield, and agronomic traits using 10 upland-adapted rice genotypes, i.e. eight... 相似文献
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Bhuminad DEVKOTA Masahiro TAKAHASHI Saori SATO Kouya SASAKI Atsushi UEKI Takeshi OSAWA Masahiro TAKAHASHI Norio YAMAGISHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(7):875-878
Blood samples were obtained sequentially from 10 dairy cows around the time of
parturition to assess plasma fluctuations in estradiol-17β (E2) levels in
association with those of several bone resorption markers. Plasma E2
concentration increased sharply a few days prepartum and decreased quickly after
parturition. In terms of bone resorption markers, the plasma level of tartrate-resistant
acid phosphatase isoform 5b (TRAP5b) rose significantly, commencing 1 week prepartum, and
was maintained at this level to a few days postpartum. The plasma concentration of
carboxyterminal collagen cross-links of type-I collagen (CTx) increased significantly
after parturition. These observations suggest that osteoclast-mediated bone resorption was
activated after parturition when plasma E2 concentrations decreased. 相似文献
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Shuhei ENJOJI Ryotaro YABE Nobuyuki FUJIWARA Shunya TSUJI Michael P. VITEK Takuya MIZUNO Takayuki NAKAGAWA Tatsuya USUI Takashi OHAMA Koichi SATO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(11):1451-1456
Canine melanoma is one of the most important diseases in small animal medicine.
Protein phosphatase 2A (PP2A), a well conserved serine/threonine phosphatase, plays a
critical role as a tumor suppressor. SET/I2PP2A is an endogenous inhibitor for PP2A, which
directly binds to PP2A and suppresses its phosphatase activity. Elevated SET protein
levels have been reported to exacerbate human tumor progression. The role of SET in canine
melanoma, however, has not been understood. Here, we investigated the potential
therapeutic role for SET inhibitors in canine melanoma. The expression of SET protein was
observed in 6 canine melanoma cell lines. We used CMeC-1 cells (primary origin) and CMeC-2
cells (metastatic origin) to generate cell lines stably expressing SET-targeting shRNAs.
Knockdown of SET expression in CMeC-2, but not in CMeC-1, leads to decreased cell
proliferation, invasion and colony formation. Phosphorylation level of p70 S6 kinase was
decreased by SET knockdown in CMeC-2, suggesting the involvement of mTOR (mammalian target
of rapamycin)/p70 S6 kinase signaling. The SET inhibitors, OP449 and FTY720, more
effectively killed CMeC-2 than CMeC-1. We observed PP2A activation in CMeC-2 treated with
OP449 and FTY720. These results demonstrated the potential therapeutic application of SET
inhibitors for canine melanoma. 相似文献
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Hajime ASADA Hirotaka TOMIYASU Yuko GOTO-KOSHINO Yasuhito FUJINO Koichi OHNO Hajime TSUJIMOTO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(6):677-684
Canine histiocytic sarcoma (HS) is an aggressive tumor type originating from histiocytic
cell lineages. This disease is characterized by poor response to chemotherapy and short
survival time. Therefore, it is of critical importance to identify and develop effective
antitumor drugs against HS. The objectives of this study were to examine the drug
sensitivities of 10 antitumor drugs. Using a real-time RT-PCR system, the mRNA expression
levels of 16 genes related to drug resistance in 4 canine HS cell lines established from
dogs with disseminated HS were determined and compared to 2 canine lymphoma cell lines
(B-cell and T-cell). These 4 canine HS cell lines showed sensitivities toward microtubule
inhibitors (vincristine, vinblastine and paclitaxel), comparable to those in the canine
B-cell lymphoma cell line. Moreover, it was shown that P-gp in the HS cell lines used in
this study did not have enough function to efflux its substrate. Sensitivities to
melphalan, nimustine, methotrexate, cytarabine, doxorubicin and etoposide were lower in
the 4 HS cell lines than in the 2 canine lymphoma cell lines. The data obtained in this
study using cultured cell lines could prove helpful in the developing of advanced and
effective chemotherapies for treating dogs that are suffering from HS. 相似文献
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Sayuri Okawa Kana Unuma Atsushi Yamada Toshihiko Aki Koichi Uemura 《Journal of toxicologic pathology》2015,28(1):37-41
The involvement of the lung during the septic systemic inflammatory response elicited by administration of lipopolysaccharide (LPS) was investigated. Eight-week-old male Sprague–Dawley rats were injected i.p. with 15 mg/kg LPS. After 24 h, the lungs were excised to evaluate the cellular responses to LPS. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF) analysis revealed that type VI collagen (ColVI) was extremely upregulated during sepsis in the rat lung within the first 24 h of LPS administration. Upregulation of ColVI protein and its mRNA was demonstrated by Western blot analysis, real time PCR, and immunohistochemistry. To the best of our knowledge, this is the first report demonstrating the activation of ColVI in the rat lung at the early stage of systemic inflammation. Activation of ColVI might be involved in sepsis-mediated lung fibrosis at an early stage. 相似文献
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Kikumi OGIHARA Ken ONDA Reiichiro SATO Yuko NAYA Hideharu OCHIAI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(1):85-88
L-type amino acid transporter
1 (LAT1), the first isotype of amino acid transport system L, transports aromatic and
branched amino acids pivotal for fundamental cellular activities such cellular growth and
proliferation. LAT1 expression was high only in the brain in contrast to its limited
distribution and low level of expression in normal tissues. We found potent LAT1
expression in canine caput epididymis by quantitative RT-PCR and Western blotting
analysis. Immnuno-histochemical examination revealed observable LAT1 in microvillous
epithelial cells. 相似文献
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Kazuyuki SUZUKI Toshio SHIMAMORI Ayano SATO Kenji TSUKANO Masakazu TSUCHIYA Jeffrey LAKRITZ 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(8):977-979
The aim of the present study was to compare the ability of the commercially available portable test system (PTSTM) to detect endotoxin activity in bovine serum, with that of the traditional LAL-kinetic turbidimetric (KT) and chromogenic (KC) assays. Prior to testing, serum samples, which were obtained from endotoxin-challenged cattle, were diluted 1:20 in endotoxin-free water and heated to 80°C for 10 min. The performance of the PTSTM was not significantly different from that of the traditional LAL-based assays. The results using PTSTM correlated with those using KT (r2=0.963, P<0.001) or KC assays (r2=0.982, P<0.001). Based on these findings, the PTSTM could be applied as a simplified system to assess endotoxin activity in bovine serum. 相似文献
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