Vigour/tolerance trade‐off in cultivated sunflower (Helianthus annuus) response to salinity stress is linked to leaf elemental composition

Developing more stress‐tolerant crops will require greater knowledge of the physiological basis of stress tolerance. Here, we explore how biomass declines in response to salinity relate to leaf traits across 20 genotypes of cultivated sunflower (Helianthus annuus). Plant growth, leaf physiological traits and leaf elemental composition were assessed after 21 days of salinity treatments (0, 50, 100, 150 or 200 mM NaCl) in a greenhouse study. There was a trade‐off in performance such that vigorous genotypes, those with higher biomass at 0 mM NaCl, had both a larger absolute decrease and proportional decrease in biomass due to increased salinity. More vigorous genotypes at control were less tolerant to salinity. Contrary to expectation, genotypes with a low increase in leaf Na and decrease in K:Na were not better at maintaining biomass with increasing salinity. Rather, genotypes with a greater reduction in leaf S and K content were better at maintaining biomass at increased salinity. While we found an overall trade‐off between sunflower vigour and salt tolerance, some genotypes were more tolerant than expected. Further analysis of the traits and mechanisms underlying this trade‐off may allow us to breed these into high‐vigour genotypes in order to increase their salt tolerance.     

An economically based evaluation index for perennial and short‐term ryegrasses in New Zealand dairy farm systems

Grazed pastures based on ryegrass species provide most of the feed for dairy cattle in New Zealand. There are many cultivars of perennial (Lolium perenne), annual and Italian (L. multiflorum), and hybrid (L. boucheanum) ryegrasses available for dairy farmers to use in pasture renewal. This study describes an index which ranks ryegrass cultivars relative to a genetic base according to the estimated economic value (EV) of seasonal dry matter (DM) traits. A farm system model was used to derive EVs (Grazed pastures based on ryegrass species provide most of the feed for dairy cattle in New Zealand. There are many cultivars of perennial (Lolium perenne), annual and Italian (L. multiflorum), and hybrid (L. boucheanum) ryegrasses available for dairy farmers to use in pasture renewal. This study describes an index which ranks ryegrass cultivars relative to a genetic base according to the estimated economic value (EV) of seasonal dry matter (DM) traits. A farm system model was used to derive EVs ($ ha^?1 calculated as change in operating profit divided by unit change of the trait) for additional DM produced in different seasons of the year in four regions. The EV of early spring DM was consistently high across all regions, whereas EV for late spring DM was moderate to low. Genotype × environment analysis revealed significant reranking of DM yield among ryegrass cultivars across regions. Hence, separate performance values (PVs) were calculated for two mega‐environments and then combined with the corresponding season and region EV to calculate the overall EV for twenty‐three perennial ryegrass and fifteen short‐term ryegrass cultivars. The difference in operating profit between the highest ranked and lowest ranked perennial ryegrass cultivar ranged from $556 ha^?1 to $863 ha^?1 year^?1 depending on region. For short‐term ryegrasses used for winter feed, the corresponding range was $394 to $478 ha^?1 year^?1. Using PV for DM yield, it was estimated that plant improvement in perennial ryegrass has added $12–$18 ha^?1 year^?1 (depending on region) operating profit on dairy farms since the mid‐1960s.     

DNA barcoding of fish eggs collected off northwestern Cuba and across the Florida Straits demonstrates egg transport by mesoscale eddies

Identifying spawning sites for broadcast spawning fish species is a key element of delineating critical habitat for managing and regulating marine fisheries. Genetic barcoding has enabled accurate taxonomic identification of individual fish eggs, overcoming limitations of morphological classification techniques. In this study, planktonic fish eggs were collected at 23 stations along the northwestern coast of Cuba and across the Florida Straits to United States waters. A total of 564 fish eggs were successfully identified to 89 taxa within 30 families, with the majority of taxa resolved to species. We provide new spawning information for Luvarus imperialis (Louvar), Bothus lunatus (Plate Fish), Eumegistus illustris (Brilliant Pomfret), and many economically important species. Data from most sites supported previously established patterns of eggs from neritic fish species being found on continental shelves and oceanic species spawning over deeper waters. However, some sites deviated from this pattern, with eggs from reef‐associated fish species detected in the deep waters of the Florida Straits and pelagic species detected in the shallow, continental shelf waters off the coast of northwestern Cuba. Further investigation using satellite imagery revealed the presence of a mesoscale cyclonic eddy that likely entrained neritic fish eggs and transported them into the Florida Straits. The technique of combining DNA‐based fish egg identification with remotely‐sensed hydrodynamics provides an important new tool for assessing the interplay of regional oceanography with fish spawning strategies.     

Monitoring water interception by crop fields from passive microwave observations

In the present work, the time variations of a wheat field microwave emission are analysed during irrigation phases. The aim of the study is to investigate the use of low-frequency microwave measurements to monitor hydrological variables over a wheat crop: surface soil moisture and water interception by the vegetation canopy. The irrigation phases were representative of rainfall or dew events. It appears that the radiometric measurements are strongly sensitive to the hydrological characteristics of the soil and of the vegetation canopy. The 1.4GHz measurements are mainly sensitive to surface soil moisture m_v, whereas the 5 GHz measurements are sensitive to both m_v and to the vegetation water content W_v. A simple model approach is implemented to retrieve simultaneously these two surface parameters. The results of this procedure show the high potential of the microwave remote sensing measurements to monitor the time variations of soil moisture and of the total vegetation water content. The value of the wheat water storage capacity is derived from the microwave measurements and is in good agreement with experimental data.     

A COMPARISON OF NUTRIENT LOSSES IN BALED HAY MADE FROM FORAGE-HARVESTED, CRIMPED OR TEDDED SWARDS WITH THOSE IN BARN-DRYING

Two experiments were conducted to study the effect on nutrient loss and feeding value of making hay by different means, using a barn-drier, a forage-harvester, a crimper and conventional tedding. Both forage-harvesting and crimping of the herbage significantly increased the rate of drying, compared with tedding alone. In Exp. 2 this effect enabled the crimped and forage-harvested hays to be baled for storage 3 days earlier than the tedded hay. The use of the forage-harvester significantly reduced the yield of herbage compared with conventional mowing. Nutrient losses from cutting until storage were highest for the forage-harvested hays, the loss of dry matter between cutting and storage in the forage-harvested hays being 39·9 and 19·3%, respectively, in Expt 1 and 2 with corresponding losses in the conventionally made hays of 7·1% and 9·3%. Crimping the hay resulted in higher nutrient losses than tedding. In Expt 2 there was a loss of dry matter of 17·5% in the conventionally made hay during an 18-week storage period, compared with 3·6% in barn-dried hay, 10·8% in forage-harvested hay and 8·8% in crimped hay.
In Expt 2, in which digestibility determinations were made, conservation decreased the total digestible nutrients and starch equivalent in all treatments. The use of the barn-drier gave the most efficient conservation of total digestible nutrients, followed by the crimped, forage-harvested and conventional treatments in that order. The starch equivalent values of hays made from the same sward were 43·0 when barn-dried, 39·8 when crimped, 38·6 after forage-harvesting and 31·6 after tedding.     

LOSSES IN THE CONSERVATION OF HEAVILY-WILTED HERBAGE SEALED IN POLYTHENE FILM IN LINED TRENCH SILOS

A conservation experiment is described in which the losses in ensiling heavily-wilted herbage, of approximately 50% dry matter, in lined trench silos, with and without a polyethylene film seal, are compared. The composition and digestibility of the silages produced in the sealed silos were comparable with those of the original herbage. The content of organic acids in the sealed silos was low, suggesting that fermentation was suppressed by the partially gas-tight seal. In the unsealed silos there was considerable spoilage, 70% of the material being inedible. In the 2 sealed silos losses of dry matter were 8·2 and 5·2% and losses of SE 11·5 and 7·8%, respectively. The losses recorded are compared with those usually encountered in lined trench silos.