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1.
Takafumi SUZUKI Naohito NISHII Satoshi TAKASHIMA Tatsuya MATSUBARA Atsushi IWASAWA Hirofumi TAKEUCHI Kohei TAHARA Tatsuyuki HACHISU Hitoshi KITAGAWA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(11):1379-1383
Polyclonal immunoglobulin (Ig) G autoantibodies against insulin have been
identified in sera of healthy cats. We purified and fractionated insulin-binding IgGs from
cat sera by affinity chromatography and analyzed affinity of insulin-binding IgGs for
insulin and their epitopes. Following the passing of fraction A, which did not bind to
insulin, insulin-binding IgGs were eluted into two fractions, B and C, by affinity
chromatography using a column fixed with bovine insulin. Dissociation constant (KD) values
between insulin-binding IgGs and insulin, determined by surface plasmon resonance analysis
(Biacore™system), were 1.64e−4 M for fraction B (low affinity IgGs) and
2e−5 M for fraction C (high affinity IgGs). Epitope analysis was conducted
using 16 peptide fragments synthesized in concord with the amino acid sequence of feline
insulin by an enzyme-linked immunosorbent assay. Fractions B and C showed higher
absorbance (affinity) of the peptide fragment of 10 amino acid residues at the
carboxyl-terminal of the B chain (peptide No. 19), followed by peptide fragments of 6 to
15 amino acid residues of the B chain (peptide No. 8). Fraction C showed a higher
absorbance to 7 to 16 amino acid residues of the B chain (peptide No. 5) compared with the
absorbance of fraction B. Polyclonal insulin-binding IgGs may form a macromolecule complex
with insulin through the multiple affinity sites of IgG molecules. Feline insulin-binding
IgGs are multifocal and may be composed of multiple IgG components and insulin. 相似文献
2.
3.
Hitoshi Kaneko Takeshi Okunishi Takashi Seto Hiroshi Kuroda Sachihiko Itoh Shinya Kouketsu Daisuke Hasegawa 《Fisheries Oceanography》2019,28(2):212-227
To clarify the effects of temperature on the recruitment of chub mackerel (Scomber japonicus) in the North Pacific, we investigated the influence of winter surface temperature (WST) on spawners at the time of maturity around the spawning grounds and the influence of ambient spring temperature on larvae using estimated temperature (ET) obtained from particle tracking experiments. We found a significant positive correlation between ET approximately 10 days following hatching and the recruitment per spawning stock biomass (RPS) after 2000. The closer (more meandering) the Kuroshio Current (KC) axis was in relation to the spawning ground, the higher (lower) the spring surface temperature and the higher (lower) RPS was in the spawning ground. In contrast, WST inside KC near the maturity/spawning ground was significantly negatively correlated with RPS. A significant negative correlation between the temperatures in winter and spring was detected in the area after 2000, when the conditions of the Pacific decadal oscillation index and the stability of the Kuroshio Extension were synchronous, indicating that KC shifted northward during this time. The reversed temperature pattern was consistent with the winter–spring movement of KC axis in the offshore direction and was correlated with the winter–spring difference in the intensity of the Aleutian low. These results suggest that the annual variation in chub mackerel recruitment after 2000 was strongly affected by the combined effects of ambient temperature because of the reversal of conditions that occurred between winter and spring around the maturity/spawning ground, which was related to the KC path. 相似文献
4.
Census and effective population sizes of white‐spotted charr (Salvelinus leucomaenis) in a fragmented landscape 下载免费PDF全文
Shoichiro Yamamoto Hitoshi Kubota Koh Hasegawa Tomoyuki Nakamura 《Ecology of Freshwater Fish》2016,25(4):612-621
Several habitat models have been proposed to predict population size for stream fishes and to guide habitat assessment and monitoring techniques. However, most models do not incorporate the potential advantage of molecular genetic markers. We conducted a field survey and microsatellite DNA analyses to quantify the relationships among genetic diversity, census/effective population size and habitat variables in fragmented populations of white‐spotted charr (Salvelinus leucomaenis). The census population size significantly increased with the stream length, the number of pools and a pool‐riffle sequence index, a proxy for channel‐unit habitat type complexity within reaches. Population density was correlated with the pool‐riffle sequence index only. Genetic diversity and effective population size were not correlated with the habitat variables or census population size. There was a lack of isolation‐by‐distance population structure in the studied populations. Our results suggest that stream length and the number of pools within reaches associated with habitat complexity are the habitat variables that explain the majority of variation in population size of white‐spotted charr. Our findings provide further evidence that census population size per se is a poor indicator of the inclusive genetic diversity within populations in a fragmented landscape. 相似文献
5.
Hitoshi Fujiie Atsushi Maruyama Masako Fujiie Michiko Takagaki Douglas J. Merrey Masao Kikuchi 《Irrigation and Drainage Systems》2011,25(1):39-60
It is well-known that major irrigation projects have a strong scale economy, handicapping irrigation development in sub-Saharan
Africa (SSA) because of the difficulty in formulating large-scale projects. Using project-level investment cost and performance
data of major and minor irrigation projects, this paper examines the causes of the economy of scale phenomenon. We find that
strong scale economy exists not only for major but also for minor projects, i.e., small- and micro-scale, projects. This is
largely because of the existence of indivisible overhead costs such as high-opportunity-cost human resources for planning,
designing and engineering management and supervision. We also find that large differences between major and minor projects
in the absolute level of overhead as well as construction costs creates a strong scale diseconomy and results in better performance
of minor projects. The advantage of minor projects holds even when their higher risk associated with the water source is taken
into consideration. We argue that there is an urgent need to promote irrigation development in SSA through developing minor
projects, and to reduce the heavy burden of overhead costs by developing the capacity of human resources at the national,
local and farmer levels in the fields of irrigation engineering, irrigation agronomy, institutional development, and micro
water management technologies. 相似文献
6.
Elisabete Yurie Sataque Ono Elaine Cunha Moreno Mario Augusto Ono Carolina Nachi Rossi Gervásio Hitoshi Saito Édio Vizoni Yoshitsugu Sugiura Elisa Yoko Hirooka 《European journal of plant pathology / European Foundation for Plant Pathology》2011,131(4):653-660
In this study the effect of different cropping systems and crop successions was evaluated on natural Fusarium sp. contamination and fumonisin levels in corn. The cropping systems consisted of a conventional and no-tillage area cultivated
with corn in the summer following either oats or fallow in the winter (2006 and 2007 growing seasons). In addition, the effect
of applying nitrogen fertilizer (0, 22.5, 45.0, 90.0 and 90.0 kg ha−1 nitrogen supplemented with potassium oxide) on fumonisin contamination was evaluated in the 2006 growing season. Fusarium sp. was detected in 90% samples in 2006 and in 100% samples in 2007. In both growing seasons, no-till corn following oats
showed the highest mean fumonisin levels and differed significantly (P < 0.05) from all the others (2006) and from conventional till corn following either oats or fallow in the winter (2007).
Fumonisin levels ranged from 0.13 to 19.52 μg g−1 (mean 6.97 μg g−1) and from 3.70 to 7.75 μg g−1 (mean 6.29 μg g−1) in no-till corn following oats from the 2006 and 2007 growing seasons, respectively. Plots treated with 0 kg ha−1 and 22.5 kg ha−1 nitrogen showed the highest mean fumonisin levels and differed significantly from those with 45.0 and 90 kg ha−1 nitrogen. Fumonisin levels correlated negatively (P < 0.05) with the nitrogen fertilization rates. Although no-till is advantageous from a soil conservation standpoint, it may
enhance the potential for fumonisin contamination in corn. 相似文献
7.
Masahide Aikawa Takatoshi Hiraki Hitoshi Mukai Kentaro Murano 《Water, air, and soil pollution》2008,190(1-4):287-297
A field survey on the concentration of chemical species in particulate matter and gaseous compounds at two monitoring sites with different site classifications (urban and rural) was conducted over three years. Total (particulate matter + gaseous compounds) concentrations at the rural site were significantly lower than those at the urban site for all species (sulfur $\left( {{\text{SO}}^{{{\text{2 - }}}}_{{\text{4}}} {\left( {\text{p}} \right)}} \right.$ and SO2(g)), nitrate ${\text{(NO}}_{{{\text{3}}^{{\text{ - }}} }} {\left( {\text{p}} \right)}$ and HNO3(g)), ammonium ${\text{(NH}}_{{{\text{4}}^{{\text{ + }}} }} {\text{(p)}})$ and ammonia (NH3(g)), and chloride (Cl? (p) and HCl (g))), which is thought to reflect classification of the site. The difference in the sulfur concentration at the urban and rural sites was characterized by the difference in SO2 (g) concentration. Further, a clear seasonality was observed for the nitrate species. The HNO3 (g) concentration was high in the summer compared with other seasons at both the urban and rural sites. The ${\text{NH}}_4^ + \left( {\text{p}} \right)$ concentration levels were approximately the same as those of NH3 (g) at both sites. The molar ratios of the particulate matter concentration to the total concentration showed different characteristics; the nitrate, ammonium and ammonia, and chloride species showed a clear seasonal variation: low in summer and high in winter and the values were similar regardless of the site. On the other hand, the sulfur species showed constant values at both the urban and rural sites, however the concentrations were significantly different for the two sites. Ammonium accounted for the largest proportion of cations in the particulate matter, regardless of the site classification. In contrast, ${\text{SO}}_4^{2 - } \left( {\text{p}} \right)$ accounted for the largest proportion of anions at the rural site, whereas ${\text{NO}}_3^ - \left( {\text{p}} \right)$ was comparable to ${\text{SO}}_4^{2 - } \left( {\text{p}} \right)$ at the urban site. Ammonia accounted for the largest proportion of all chemical species at both sites. Seasonal analysis of the proportional distribution in particulate matter and gaseous compounds provides information on atmospheric conditions. 相似文献
8.
Aoshima H Okita Y Hossain SJ Fukue K Mito M Orihara Y Yokoyama T Yamada M Kumagai A Nagaoka Y Uesato S Hara Y 《Journal of agricultural and food chemistry》2005,53(6):1955-1959
Recently, 3-O-octanoyl-(+)-catechin (OC) was synthesized from (+)-catechin (C) by incorporation of an octanoyl chain into C in the light of (-)-epicatechin gallate (ECg) and (-)-epigallocatechin gallate (EGCg), which are the major polyphenols found in green tea and have strong physiological activities. OC was found to inhibit the response of ionotropic gamma-aminobutyric acid (GABA) receptors (GABA(A) receptors) and Na+/glucose cotransporters expressed in Xenopus oocytes in a noncompetitive manner more strongly than does C. OC also induced a nonspecific membrane current and decreased the membrane potential of the oocyte, and thus death of the oocyte occurred even at lower concentrations than that induced by C or EGCg. Although EGCg produced H2O2 in aqueous solution, OC did not. This newly synthesized catechin derivative OC possibly binds to the lipid membrane more strongly than does C, Ecg, or EGCg and as a result perturbs the membrane structure. 相似文献
9.
Preparation of phytate-removed deamidated soybean globulins by ion exchangers and characterization of their calcium-binding ability. 总被引:3,自引:0,他引:3
Hitomi Kumagai Shigeo Ishida Atsushi Koizumi Hidetoshi Sakurai Hitoshi Kumagai 《Journal of agricultural and food chemistry》2002,50(1):172-176
Phytate-removed deamidated soybean globulins were prepared using ion-exchange resins to provide them with a functional property to enhance calcium absorption in the body. The phosphorus level was reduced from 45 to 20 micromol/g using 0.05 g/mL of AE-4, an anion-exchange resin with a (2-hydroxyethyl)dimethylammonium group, in 0.2% soybean globulin solution for 1 h at 4 degrees C, and 90-92% of the phosphorus in defatted soybeans could be removed. As for deamidation, CE-4, a cation-exchange resin of the carboxylate type, showed a much higher deamidation activity than CE-1 and CE-2, cation-exchange resins of the sulfonate type. No peptide bond hydrolysis was observed for any cation-exchange resin treated at 4 degrees C. There was no significant difference in the amount of acid amide deamidated at temperatures between 4 and 50 degrees C. The deamidation level was able to increase to 73% using 0.10 g/mL of CE-4 in a 0.2% soybean globulin solution for 6 h at 4 degrees C. The amount of calcium bound to the soybean globulins decreased with removal of the phytate but increased with deamidation. 相似文献
10.
Hideaki Endo Eiji Takahashi Masataka Murata Hitoshi Ohnuki Huifeng Ren Wakako Tsugawa Koji Sode 《Fisheries Science》2010,76(4):687-694
A wireless biosensor system was developed for the continuous measurement of blood glucose levels in flatfish. The biosensor
was implanted in the interstitial fluid under the scleral surface of the eyeball (EISF) to investigate the relationship between
EISF and blood glucose levels. EISF glucose levels were found to be correlated with those in the blood and to be approximately
the same as blood glucose levels in the range of 7–25 mg dl−1. A needle-type biosensor was prepared for the continuous EISF glucose monitoring in flatfish. A working electrode was constructed
using platinum iridium wire, and glucose oxidase was immobilized to the electrode. The biosensor was inserted into the EISF
of flatfish for sensor implantation. A 650-mV potential (vs. Ag/AgCl) was applied by a wireless potentiostat to the working
electrode for the amperometric glucose measurement. We investigated whether glucose in the EISF can be determined in vivo.
The estimated glucose levels using a one-point calibration method were correlated with actual blood glucose levels. In conclusion,
using a wireless biosensor system, we were able to monitor blood glucose levels in flatfish under free-swimming conditions
for 16 h. 相似文献