Serum amyloid A protein concentration measured by radial immunodiffusion in Abyssinian and non-Abyssinian cats

Serum amyloid A (SAA) protein concentration was determined by use of radial immunodiffusion (RID) in 4 groups of cats: Abyssinian cats with amyloidosis, healthy Abyssinian cats without clinical evidence of amyloidosis, hospitalized non-Abyssinian cats, and clinically normal non-Abyssinian cats. Mean SAA concentration in Abyssinian cats with amyloidosis was significantly (P = 0.05) higher than mean SAA concentration in healthy Abyssinian cats without clinical evidence of amyloidosis and in hospitalized non-Abyssinian cats. Mean SAA concentration in clinically normal non-Abyssinian cats was significantly (P = 0.05) lower than mean SAA concentration in healthy Abyssinian cats without clinical evidence of amyloidosis and in hospitalized non-Abyssinian cats. Affected and healthy Abyssinian cats, however, could not reliably be distinguished on the basis of SAA concentration, because of the wide range of SAA values in these 2 groups of cats.     

Extramedullary plasmacytoma and immunoglobulin-associated amyloidosis in a cat

Extramedullary plasmacytoma with immunoglobulin-associated amyloidosis was diagnosed in a 10-year-old cat. The primary tumor was a large, circumferential mass of the right tarsal region. Metastasis developed in the regional lymph nodes, spleen, and liver. Permanganate-resistant amyloid deposits were associated with plasma cells in the primary and metastatic tumors. Treatment with prednisone and melphalan had little effect on the progression of the disease, and the cat died four weeks later.     

Resistance to gentamicin and amikacin of gram-negative organisms isolated from horses

Resistance of gram-negative bacteria to gentamicin has become an increasingly common problem among clinical isolates from human beings. Susceptibility of isolates from horses to gentamicin and amikacin was evaluated for the period from July, 1983 to June, 1985. All isolates of Escherichia coli, and species of Enterobacter, Klebsiella, Proteus, and Pseudomonas examined were susceptible to amikacin, except 2 of the 46 Pseudomonas isolates. In contrast, 13 to 50% of isolates were resistant to gentamicin. Escherichia coli, and Klebsiella, Proteus, and Enterobacter species isolates were highly significantly more susceptible to amikacin (P less than 0.01) than to gentamicin. Pseudomonas spp (P = 0.13) were not significantly different in susceptibility to the 2 drugs. There was significant variation among genera in their susceptibility to gentamicin (P = 0.002), primarily because of the frequency of resistance in isolates of Klebsiella spp and Proteus spp, compared with the other 3 organisms (E coli, Enterobacter spp, and Pseudomonas spp). There was no significant difference of susceptibility to amikacin among the genera studied (P = 0.06).     

Total mercury levels in selected human tissues, Idaho-1973-74(1,2)

Total mercury levels were determined in human tissues taken at autopsy from six hospitals in the three basic geographical areas of Idaho. Of the 242 specimens analyzed, 76 percent contained detectable mercury. Levels were compared with respect to the age, sex, and geographic residence of autopsied individuals. Mean levels detected were 1.04 ppm in kidney tissue, 0.34 ppm in liver, and 0.08 ppm in brain. Mean mercury levels for the three geographical areas were: southeastern Idaho, 0.22 ppm; southwestern Idaho, 0.80 ppm; and northern Idaho, 0.43 ppm. The relatively high means in southwestern Idaho specimens may be related to the preponderance of natural cinnabar deposits in that portion of the State. Mercury levels were higher in women than men for all tissues in both the southwestern and northern areas, but the reverse was true in the southeast. Data were compared with findings of other investigators in an attempt to arrive at background levels of total mercury residues in human tissues.     

植物育种的物理学方法

介绍了草原生态恢复中选育优良牧草种子的一些物理学方法,包括电场、磁场、物理辐射、激光、离子束和交变应力等在植物的生物学效应、诱变育种和转基因技术中的应用.     

中国水牛瘤胃纤毛虫分布与挥发性脂肪酸的研究

对中国广西西林水牛和摩拉水牛瘤胃纤毛虫种类分布进行了鉴定检测,并测定了其瘤胃液挥发性脂肪酸浓度,同时进行了比较研究。全部检出17属63种25型瘤胃纤毛虫,比以前进行的8种反刍动物瘤胃纤毛虫分布研究中出现的总数和每个宿主个体中出现的平均纤毛虫种数多,种类构成最复杂,其纤毛虫构成特点类似于东南亚地区水牛瘤胃纤毛虫构成特点。其中广西西林水牛中检出了Entodinium biconcavumsp n,是至今从未鉴定记载过的认为是新种的特殊种类。广西西林水牛检出13属54种20型、摩拉水牛检出16属45种11型瘤胃纤毛虫。广西西林水牛瘤胃纤毛虫平均密度为2.13×105/mL,摩拉水牛瘤胃纤毛虫密度为3.43×105/mL。中国水牛瘤胃纤毛虫属别构成中Entodinium属出现率最高,但两个水牛品种间存在明显差别,其他属别构成也不尽一致。这与不同品种原来的地理环境分布与饲养管理条件有关。广西西林水牛瘤胃总VFA浓度为75.60 mmol/mL,摩拉水牛的为58.74 mmol/mL。瘤胃总VFA含量中均为乙酸含量最高,属于典型的粗饲料发酵类型。     

EIAV Rev蛋白拮抗eqTRIM5α介导的AP-1信号通路的机制研究

为探究马传染性贫血病毒(EIAV)附属蛋白Rev负调控Tripartite motif-containing protein 5α(TRIM5α)介导的AP-1信号通路的机制,本研究将pEIAV-Rev-HA和pcDNA3.1质粒分别与含TRIM5α基因的质粒及pGL3-AP-1-Luc(AP-1报告质粒)共转染HEK 293T细胞,采用荧光素酶试验检测Rev对TRIM5α激活的AP-1信号通路的影响;将pEIAV-Rev-HA和pcDNA3.1质粒分别与含TAK1、TAB2、P38和c-Jun基因的质粒及pGL3-AP-1-Luc共转染HEK 293T细胞,采用荧光素酶试验检测Rev对TRIM5α下游转导分子(TAK1、TAB2、P38、c-Jun)激活的AP-1信号通路的影响;将pEIAV-Rev-HA和pcDNA3.1质粒分别与含TAK1、TAB2、P38基因的质粒共转染HEK293T细胞,利用western blot试验分别检测TAK1、TAB2、P38的表达水平;将pEIAV-Rev-HA和pcDNA3.1质粒分别与含P38基因的质粒共转染HEK 293T细胞后加入蛋白酶体抑制剂MG132,利用western blot检测P38蛋白的表达情况。结果显示,共转染EIAV-Rev-HA实验组中TRIM5α对AP-1的激活倍数为0.4,而共转染pcDNA3.1对照组中相应的激活倍数为26.0;共转染pEIAV-Rev-HA实验组中,TAK1、TAB2、P38和c-Jun对AP-1信号通路的激活倍数分别为7.7、0.1、0.6、9.8,而共转染pcDNA3.1对照组中对AP-1信号通路的激活倍数分别为60.0、1.5、6.3、12.0;转染pEIAV-Rev-HA+pP38-Flag组与转染pcDNA3.1+pP38-Flag组相比,前者P38蛋白的表达量显著降低;加入蛋白酶体抑制剂组则恢复了P38蛋白的表达。上述结果表明,EIAV Rev显著下调eqTRIM5α及其下游转导分子TAK1、TAB2、P38激活的AP-1信号通路,但不显著下调c-Jun激活的AP-1信号通路;EIAV Rev通过蛋白酶体途径降解P38蛋白的表达而抑制eqTRIM5α激活的AP-1信号通路。本研究结果为理解EIAV与宿主蛋白相互作用提供参考依据。     

微细木质粉尘静电除尘器的设计

近年来,随着人们对环境质量的要求逐渐提高,木材工业除尘日益得到重视。众所周知,木材工业是产生粉尘的大户,如不采取有效的除尘措施,产生的粉尘不但会造成极大的环境污染,而且还会对工人的身体健康、产品质量、设备的精度和使用年限及生产的安全造成危害。目前,在木材工业中,广泛采用的多是离心力除     

Infection of human enterovirus 71 in immunosuppressed rhesus monkeys

AIM: To investigate the infection process of human enterovirus 71 (EV71) in a rhesus monkey model of immunosuppression.METHODS: The monkey immunosuppression model was established by oral administration of cyclosporin A at a daily dose of 15 mg/kg for 7 days. Fourteen days after inoculation of EV71 FY23 strain via respiratory and digestive tracts, the infectious monkeys were sacrificed, and then the pathological changes of systemic organs were observed and the viral analysis in different tissues was performed.RESULTS: Compared with the experimental group without cyclosporin A, more positive results were observed in the immunosuppressed monkeys, such as more severe clinical symptoms, higher viral load/antigen expression and more pathological changes in the organs.CONCLUSION: The findings of the viral multiplication in the host provide evidence for evaluation of EV71 vaccine, and also indicate the delimitation for the population using EV71 vaccine in future.     

磁化水在农作物上应用的研究进展

针对磁化水对农业生产的影响,简述了国内外关于磁化水应用的研究动态和发展趋势,介绍了磁化水在促进作物种子萌发、增强抗逆性以及提高产量和改善品质方面的研究进展。