排序方式: 共有8条查询结果,搜索用时 15 毫秒
1
1.
Philippe Régnier Jorge Bastias Violeta Rodriguez-Ruiz Noelia Caballero-Casero Carmen Caballo Dolores Sicilia Axelle Fuentes Murielle Maire Michel Crepin Didier Letourneur Virginie Gueguen Soledad Rubio Graciela Pavon-Djavid 《Marine drugs》2015,13(5):2857-2874
Astaxanthin, a powerful antioxidant, is a good candidate for the prevention of intracellular oxidative stress. The aim of the study was to compare the antioxidant activity of astaxanthin present in two natural extracts from Haematococcus pluvialis, a microalgae strain, with that of synthetic astaxanthin. Natural extracts were obtained either by solvent or supercritical extraction methods. UV, HPLC-DAD and (HPLC-(atmospheric pressure chemical ionization (APCI)+)/ion trap-MS) characterizations of both natural extracts showed similar compositions of carotenoids, but different percentages in free astaxanthin and its ester derivatives. The Trolox equivalent antioxidant capacity (TEAC) assay showed that natural extracts containing esters displayed stronger antioxidant activities than free astaxanthin. Their antioxidant capacities to inhibit intracellular oxidative stress were then evaluated on HUVEC cells. The intracellular antioxidant activity in natural extracts was approximately 90-times higher than synthetic astaxanthin (5 µM). No modification, neither in the morphology nor in the viability, of vascular human cells was observed by in vitro biocompatibility study up to 10 µM astaxanthin concentrations. Therefore, these results revealed the therapeutic potential of the natural extracts in vascular human cell protection against oxidative stress without toxicity, which could be exploited in prevention and/or treatment of cardiovascular diseases. 相似文献
2.
Sarah Bonnet Nadine Brisseau Axelle Hermouet Maggy Jouglin Alain Chauvin 《Veterinary research》2009,40(3)
Babesia sp. (EU1), first characterized in 2003, has been implicated in human cases of babesiosis in Italy, Austria and Germany. It has been identified in roe deer and in its suspected tick vector, Ixodes ricinus, in several European countries. The aim of the present study was to validate the competence of I. ricinus as a vector of Babesia sp. (EU1) via experimental infections. For this purpose, a parasite strain isolated from roe deer was cloned in sheep erythrocytes. After experimental infections, parasite DNA was successfully amplified by PCR in both eggs and larvae originating from infected I. ricinus females and in the salivary glands of females exposed to Babesia sp. (EU1) as nymphs. We also demonstrate that infected females were able to transmit parasite DNA during a new blood meal. Together with previous epidemiological studies, these results validate I. ricinus as a competent vector for Babesia sp. (EU1). 相似文献
3.
Eucalypt (Eucalyptus spp.) tree farming is a source of income for many smallholders in developing and emerging countries and critical to the resource
supply of many pulp and paper companies. These companies rely on smallholders adopting tree farming, sometimes by offering
a contract. This paper reports a study from four regions of Thailand, where smallholder eucalypt tree farming is important,
which investigated what characteristics of smallholders were associated with greater adoption of tree farming. A total of
461 eucalypt tree farmers and 171 non-tree farmers were randomly selected and surveyed in these regions, using a door-to-door
household survey. A logit analysis corroborated hypotheses about the drivers of adoption. Qualitative analyses were used to
inform interpretation of the quantitative results and shed light on the role of eucalypt tree farming in smallholders’ livelihood.
Results demonstrate that those with suitable land available are more likely to adopt eucalypt tree farming than others. In
addition, perception of land tenure security matters in the adoption of tree growing, but holding a formal land tenure document
does not. Adoption of eucalypt tree farming in Thailand is not part of a land use intensification strategy. Instead, eucalypts
are used as an alternative crop for low productivity land, on which eucalypts are the most profitable crop. Eucalypt tree
farming also gives smallholders an opportunity to diversify their income. In addition, this alternative land use has the advantage
of requiring low labour inputs between planting and harvest. This is particularly advantageous for many tree growers who have
off-farm income or rely on hired labour for farming their land. 相似文献
4.
The clinical and microbial efficacy of antimicrobial treatments of avian colibacillosis was studied, using an experimental model on chickens previously inoculated with multiresistant commensal Escherichia coli strains. One E. coli with pMG252 plasmid containing bla(FOX5) and qnrA1 genes and another E. coli with pMG298 plasmid containing bla(CTX-M15) and qnrB1 genes were first orally inoculated to chickens Both isolates were also resistant to chloramphenicol, sulphamethoxazole, trimethoprim, streptomycin, gentamicin, kanamycin, and tetracycline. The birds were then experimentally infected with an avian pathogenic E. coli (APEC), via the air sac. Treatments (oxytetracycline (OTC), trimethoprim-sulfadimethoxin (SXT), amoxicillin (AMX) or enrofloxacin (ENR) were then offered at the therapeutic doses. Symptoms, lesions in dead or sacrificed birds, and isolation and characterization of APEC from internal organs were studied. Results showed that OTC, SXT or ENR treatments could control the pathology. AMX worsened the disease, possibly due to endotoxin shock. All APEC re-isolated from internal organs showed the same antimicrobial susceptibility as the APEC inoculated strain, except for one APEC isolate from an infected OTC-treated bird, which acquired tetracycline resistance only, and one APEC isolate recovered from the air sacs of a chicken in the infected SXT-treated group, which acquired the pMG252 plasmid and became multi-resistant. Thus three antimicrobials could control the disease but the experimental model enabled, to our knowledge, the first observation of plasmid transfer from a bacterium of the intestinal tract to a pathogenic isolate from the respiratory tract. 相似文献
5.
Eucalypt (Eucalyptus spp.) tree farming is a source of income for many smallholders in developing and emerging countries and critical to the resource supply of many pulp and paper companies. These companies rely on smallholders adopting tree farming, sometimes by offering a contract. This paper reports a study from four regions of Thailand, where smallholder eucalypt tree farming is practiced, which investigated the financial performance of contract eucalypt tree farming for smallholders. A total of 82 contract eucalypt tree farmers and 81 independent tree farmers were systematically selected and surveyed in these regions, using door-to-door personal interviews. Focus groups and key informant interviews were also used to gain in-depth understanding of the issues. An investment appraisal analysis was used to determine and evaluate comparative financial returns from various cutting cycle periods, through computation of the net present value (NPV). There was no evidence that contracts increase the NPV of tree growing. It could be that tree growers entered into a contractual agreement with the expectation of higher benefits and the importance of this expectation diminishes as the crop matures, and tree farmers appreciate the strong market for eucalypt wood in Thailand. Contracts do not increase inequalities in terms of income but they might increase inequalities in terms of knowledge, because farmers with more land have better access to new knowledge provided by the contracting company. 相似文献
6.
Renaud?IoosEmail author Claude?Husson Axelle?Andrieux Pascal?Frey 《European journal of plant pathology / European Foundation for Plant Pathology》2005,112(4):323-335
Since the 1990s, a new Phytophthora species hybrid has been jeopardizing the natural population of alders throughout Europe. This new Phytophthora, P. alni, has been suggested as a natural hybrid between two closely related species of Phytophthora. Little is known about the epidemiology of this pathogen, because its direct isolation is not always satisfactory. In this study we developed three pairs of Polymerase Chain Reaction (PCR) primers derived from Sequence Characterized Amplified Regions (SCAR) that allow discrimination among the three subspecies of P. alni: P. alni subsp. alni, P. alni subsp. uniformis and P. alni subsp. multiformis. These molecular tools were successfully used to detect P. alni directly in different substrates such as infested river water and soil, and necrotic alder bark, without the need for any prior baiting or isolation stages. An Internal Amplification Control (IAC) was included to help discriminate against false negative samples due to the potential presence of inhibitory compounds in DNA extracts. These molecular tools should be useful for epidemiological studies on this emerging disease. 相似文献
7.
Gan Q Ferrand Y Bao C Kauffmann B Grélard A Jiang H Huc I 《Science (New York, N.Y.)》2011,331(6021):1172-1175
Dynamic assembly is a powerful fabrication method of complex, functionally diverse molecular architectures, but its use in synthetic nanomachines has been hampered by the difficulty of avoiding reversible attachments that result in the premature breaking apart of loosely held moving parts. We show that molecular motion can be controlled in dynamically assembled systems through segregation of the disassembly process and internal translation to time scales that differ by four orders of magnitude. Helical molecular tapes were designed to slowly wind around rod-like guests and then to rapidly slide along them. The winding process requires helix unfolding and refolding, as well as a strict match between helix length and anchor points on the rods. This modular design and dynamic assembly open up promising capabilities in molecular machinery. 相似文献
8.
1