Effect of phosphorus fertilizer application on the performance of maize/soybean intercrop in the southern Guinea savanna of Nigeria

Field experiments were conducted at the Teaching and Research Farm, Ladoke Akintola University of Technology, Ogbomoso, Nigeria in 2007 and 2008 to determine the effects of phosphorus fertilizer application on performance of intercropped maize and soybean. The experiments, arranged as a split plot in a randomized complete block design, replicated four times. A cropping system with sole maize, sole soybean and maize/soybean intercrop formed the main plot treatments while P rates with 0, 15 and 30 kg P₂O₅ ha^?1 were the subplot treatments. For both years, neither P fertilizer application nor cropping systems had a significant effect on maize grain yield. However, soybean grain yield was significantly higher (92.3% in 2007 and 44.5% in 2008) under sole cropping than under maize/soybean intercropping. On average, N fixed by soybean increased with the increase in P rate (from 51.8% without P to 60.5% with 30 P), but there was no significant difference in N fixed by sole soybean and soybean/maize intercrop. However, the interaction effect on N fixed between cropping systems and P rates was significant (P ≤ 0.05). N, P and K contents in maize grain were significantly higher (>100%) in intercropped maize than in sole maize. The cropping systems had no significant effect on post-harvest soil chemical characteristics. The land equivalent ratio was 1.52 in 2007 and 1.78 in 2008. The result shows that in utilizing legumes for N enrichment, the alleviation of P deficiency can enhance N₂-fixation by legumes. Furthermore, P replenishment in a maize/soybean intercrop may improve maize grain quality even though yield is not increased.     

Effects of leguminous plant residues and NPK fertilizer application on the performance of yam (Dioscorea rotundata ‘c.v.’ ewuru) in south-western Nigeria

The effects of cultivating and incorporating residues of previous tropical kudzu (Pueraria phaseoloides) and soybean (Glycine max) with application of NPK fertilizer on yam performance were evaluated at the teaching and research farm, LAUTECH, Nigeria. There were nine treatments: incorporation of legume residues (5 t DM ha^?1), application of recommended fertilizer rate for yam (90–50–75 kg NPK ha^?1) in the zone or 50% of recommended rate (45–25–37.5 kg NPK ha^?1), alone and in combination with residues and a control without residues or fertilizer in a randomized complete block design. Cultivation of previous legumes reduced soil nematode population (>200%) compared with no legumes. For both years, application of Pueraria residues improved tuber yield by an average of 15.8% compared with control. Fertilizer application enhanced arbuscular mycorrhizal (AM) colonization of yam roots but AM colonization was lower (～50%) in plots where Pueraria residues were incorporated compared with other plots. Combined application of plant residues with fertilizer improved soil organic carbon, total N, exchangeable Ca and Mg compared with application of NPK fertilizer. From these results, it is concluded that half of the recommended NPK rate may be adequate and incorporation of residues with reduced NPK fertilizer application may be a sustainable soil fertility management option for continuous yam production.     

The present status of biological control of Xanthium (Compositae)

Abstract

The economic threshold level of the mustard aphid, Lipaphis erysimi (Kaltenbach) was determined on the radish seed crop, var Punjab Sufed. Spraying oxydemeton methyl at 300 g a.i./ ha, was monitored at arbitrary set aphid levels from 25 to 150 aphids/plant. The maximum cost benefit ratio (1: 13.1) was achieved at an aphid level of 50 per plant, requiring three sprays. Spraying in the middle of February was the most crucial, as delay of 7 days from this stage resulted in significant decreases in yield in the fixed spray schedule and at the 75‐aphid level.     

Production of recombinant capsid protein of Macrobrachium rosenbergii nodavirus (r‐MCP43) of giant freshwater prawn,M. rosenbergii (de Man) for immunological diagnostic methods

White tail disease (WTD) caused by Macrobrachium rosenbergii nodavirus (MrNV) and extra small virus (XSV) is a serious problem in prawn hatcheries. The gene for capsid protein of MrNV (MCP43) was cloned into pRSET B expression vector. The MCP43 protein was expressed as a protein with a 6‐histidine tag in Escherichia coli GJ1158 with NaCl induction. This recombinant protein, which was used to raise the antiserum in rabbits, recognized capsid protein in different WTD‐infected post‐larvae and adult prawn. Various immunological methods such as Western blot, dot blot and ELISA techniques were employed to detect MrNV in infected samples using the antiserum raised against recombinant MCP43 of MrNV. The dot blot assay using anti‐rMCP43 was found to be capable of detecting MrNV in WTD‐infected post‐larvae as early as at 24 h post‐infection. The antiserum raised against r‐MCP43 could detect the MrNV in the infected samples at the level of 100 pg of total protein. The capsid protein of MrNV estimated by ELISA using anti‐rMCP43 and pure r‐MCP43 as a standard was found to increase gradually during the course of infection from 24 h p.i. to moribund stage. The results of immunological diagnostic methods employed in this study were compared with that of RT‐PCR to test the efficiency of antiserum raised against r‐MCP43 for the detection of MrNV. The Western blot, dot blot and ELISA detected all MrNV‐positive coded samples as detected by RT‐PCR.     

Biochemical changes and tissue distribution of Enterocytozoon hepatopenaei (EHP) in naturally and experimentally EHP‐infected whiteleg shrimp,Litopenaeus vannamei (Boone, 1931), in India

Stunted growth in pond‐reared Litopenaeus vannamei was observed in different farms located in Tamil Nadu and Andhra Pradesh, India. No mortality was associated with stunted growth. PCR assay on these samples revealed the presence of Enterocytozoon hepatopenaei (EHP) in stunted shrimp. Tissue distribution of EHP in naturally and experimentally infected shrimp was studied by PCR and histology. Histological examination revealed the presence of EHP in hepatopancreas and gut, but not in other organs. The PCR assay revealed the presence of EHP in all the organs tested in both naturally and experimentally infected shrimp. Healthy shrimp were challenged with E. hepatopenaei by intramuscular injection and oral route, and no mortality was observed in both routes after 30 days post‐challenge. Different developmental stages of the microsporidian parasite were observed in the hepatopancreatic epithelial cells. Biochemical parameters such as total protein, albumin, aspartate transaminase (AST), alanine transaminase (ALT) and alkaline phosphatase were measured in the haemolymph of naturally and experimentally EHP‐infected shrimp. All biochemical parameters mentioned were found to be significantly higher in EHP‐infected shrimp when compared to normal shrimp. This is the first report relating AST and ALT levels to EHP infection in naturally and experimentally infected shrimp.     

Development and characterization of five novel cell lines from snubnose pompano,Trachinotus blochii (Lacepede, 1801), and their application in gene expression and virological studies

Five novel permanent cell lines have been established from gill, heart, kidney, eye and fin of snubnose pompano, Trachinotus blochii. They were designated as snubnose pompano gill (SPG), snubnose pompano heart (SPH), snubnose pompano kidney (SPK), snubnose pompano eye (SPE) and snubnose pompano fin (SPF), respectively. All these cell lines were characterized and cryopreserved successfully at different passage levels. Cell lines were passaged every alternate day; SPG, SPH, SPK, SPE and SPF cell lines attained passage levels of 68, 74, 82, 79 and 106, respectively, since the initiation of their development in 2019. The cell lines grew well in Leibovitz's 15 medium containing 15% foetal bovine serum at 28°C. Immunophenotyping of the cell lines revealed the presence of fibronectin and pancytokeratin. No mycoplasma contamination was found. The transfection study revealed the gene expression efficiency of these cell lines by expressing the green fluorescent protein (GFP). The authentication on origin of cell lines from T. blochii was confirmed by amplification of species-specific mitochondrial cytochrome oxidase I gene. The results showed the susceptibility of these cell lines to fish nodavirus (FNV) and tilapia lake virus (TiLV) and resistance to cyprinid herpesvirus 2 (CyHV-2). The FNV infection in the cell lines was confirmed by RT-PCR, Western blot, ELISA and immunocytochemistry, while TiLV infection was confirmed by RT-PCR assay. These results revealed that these cell lines are suitable for virological and foreign gene expression studies.     

Differential response of cowpea lines to aluminum and phosphorus application

Two pot experiments were conducted, one to evaluate the levels of tolerance of fifteen cowpea [Vigna unguiculata (L.) Walp] lines to aluminum (Al) application, and the second to determine the effect of phosphorus (P) addition on the performance of Al‐tolerant lines (IT 91K‐93–10, IT 93K‐2046–1, and IT 90K‐2 77–2) and Al‐sensitive lines (IT 86D‐719, IT 90K‐284–2, and IT 89KD‐349) in an Alfisol with Al amendment. Fourteen of the fifteen lines tested showed decreased root biomass (between 19 to 81% reduction) with Al addition, but this effect was significant for eight of them. Fewer lines showed decreased shoot biomass and grain yield with Al application. Despite little change in nodule number following Al application, there was a significant decrease in nodule weight (between 24 and 53% reduction) for nearly all lines. Phosphorus fertilization increased shoot and root biomass, grain yield, nodule number, and weight, and nitrogen (N) and P content of nearly all lines. Al‐tolerant lines showed higher response in shoot and root biomass and nodulation to P fertilization than Al‐sensitive lines, with the highest response from IT 90K‐277–2. Increase in shoot dry weight as a result of P fertilization was from 64 to 107% for Al‐tolerant lines and from 44 to 48% for the Al‐sensitive lines, and increase in root dry weight was from 46 to 86% for the Al‐tolerant lines and from 7 to 42% for the Al‐sensitive lines. Results of these trials indicated that lines IT 91K‐93–10, IT 93K‐2046–1, and IT 90K‐277–2 have potential for good performance in soil with Al toxicity problems, and that cowpea lines with inherent genetic tolerance to Al will give higher response to P fertilization when grown in soil with Al toxicity problems.     

Effects of nutrients omission on maize growth and nutrient uptake in three dominant soil types of southwestern Nigeria

Abstract

A pot experiment was conducted to determine most limiting nutrients for maize performance using nutrient omission treatments in three soil types of southwestern Nigeria. There were six treatments; full nutrient [120?kg nitrogen (N)/ha, 40?kg phosphorus (P)/ha, 80?kg potassium (K)/ha, 10?kg molybdenum (Mo)/ha, and 5?kg zinc (Zn)/ha]; full nutrient minus N, P, K, Mo, and Zn including control was replicated thrice. Treatments were arranged as split plot in a complete randomized design. Data were collected on growth parameters, shoot, root dry weights, and NPK uptakes. Data were subjected to analysis of variance and means separated using LSD_0.05. Majeroku and Egbeda soils and full nutrient supported better maize growth and NPK uptakes. Shoot weight was higher in Egbeda while root weight was higher in Itagunmodi soil. Phosphorus was the most limiting in Egbeda and Itagunmodi soils, and nitrogen in Majeroku soil. In conclusion, maize growth, nutrient uptake and most limiting nutrient varied with soil types.     

Immunoblotting study of the antigenic relationships among eight serogroups of Leptospira.

Seven strains of Leptospira interrogans belonging to seven different serogroups, and one strain of Leptospira biflexa were analysed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) with gradient gels and immunoblotting with hyperimmune rabbit sera raised against each strain. The molecular masses of the proteins were calculated with a polynomial regression model. The SDS-PAGE patterns of the L. interrogans strains were similar and characterized by 24 common bands. This profile was not found for L. biflexa. The immunoblots obtained either with the seven anti-L. interrogans sera or the anti-L. biflexa serum allowed a clear distinction between the two species. Taken as a whole, the L. interrogans strain patterns revealed by the seven anti-L. interrogans sera were similar, sharing eight common major bands. A serovar- or serogroup-specific antigenic zone, ranging from 21 to 26 kDa, was also identified.