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Ontogeny of digestive and immune system organs of larval and juvenile kelp grouper Epinephelus bruneus reared in the laboratory 总被引:3,自引:0,他引:3
Keitaro KATO Katsuya ISHIMARU Yoshifumi SAWADA Junichi MUTSURO Shigeru MIYASHITA Osamu MURATA Hidemi KUMAI 《Fisheries Science》2004,70(6):1061-1069
ABSTRACT: A histological examination was made of the ontogenetic development of the digestive and immune systems of the larval and juvenile kelp grouper Epinephelus bruneus reared in the laboratory. The liver, gall bladder, pancreas and the demarcating region between the intestines and rectum were formed within 3 days post-hatch (dph). During the preflexion phase (within 16 dph), revolution of the intestine concluded, and pharyngeal teeth and the mucous cells of the esophagus were differentiated. In the transitional period to the juvenile stage (25 dph), the blind sac of the stomach, gastric glands and pyloric caeca began to form. From the viewpoint of the differentiation phase of the adult-type digestive system, the kelp grouper is similar to Heterosomata, hitherto reported. The primordial thymus, kidney and spleen were present at 12, 1 and 6 dph, and the small lymphocytes in these lymphoid organs appeared at 21, 30 and 33 dph, respectively. The developmental sequence of the lymphoid organs and the appearance ages of the lymphoid organs and small lymphocytes in the lymphoid organs in the kelp grouper are similar to those of other marine fish previously reported, except for the Pacific bluefin tuna Thunnus orientalis . 相似文献
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Yoshiro ENDO Takeru TSUCHIYA Takaya OMURA Kenji NAKAI Kenji KOROSUE Mutsuki ISHIMARU Yuhiro ISHIKAWA Seiji HOBO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(1):75-79
The present study evaluated
the effects of single-dose marbofloxacin in protecting horses against fever associated
with transportation using 48 healthy Thoroughbreds. All horses were premedicated with
interferon-α (0.5 U/kg, sublingually, every 24 hr) for 2 days before transportation and on
the day of transportation. Horses were randomly assigned to receive marbofloxacin (2
mg/kg, IV, once; MRFX group), enrofloxacin (5 mg/kg, IV, once; ERFX group) or saline (0.9%
NaCl) solution (10 ml, IV, once; control group) ≤1 hr before being
transportation. Each group contained 16 horses (8 males, 8 females). Horses were
transported 1,210 km using commercial vans over the course of approximately 26 hr.
Clinical examinations and hematologic analyses were performed on all horses both before
and after transportation. Post-transportation neutrophil to lymphocyte ratios were
significantly lower in horses in the MRFX group compared with the control horses. The
serum amyloid A levels were significantly lower in horses in the MRFX group and ERFX group
compared with the control horses. Regarding the post-transportation rectal temperatures,
fever was detected in 0 horses and 1 horse in the MRFX and ERFX groups, respectively,
whereas fevers exceeding 39.1°C were detected in 2 horses in the control group.
Additionally, the number of essential post-transportation treatments provided by
veterinarians was reduced 3-fold in the MRFX and ERFX groups compared with the saline
group. MRFX provided ERFX-like protection against fever associated with long-distance
transportation, yielding significantly better protection than saline. Administration of
MRFX just before transportation deserves a further study for efficacy in preventing horse
fever associated with transportation. 相似文献
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Takashi KOZASA Yuri ABE Kazuya MITSUHASHI Tomokazu TAMURA Hiroshi AOKI Masatoshi ISHIMARU Shigeyuki NAKAMURA Masatoshi OKAMATSU Hiroshi KIDA Yoshihiro SAKODA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(5):511-518
The Exaltation of Newcastle disease virus (END) phenomenon is induced by the
inhibition of type I interferon in pestivirus-infected cells in vitro,
via proteasomal degradation of cellular interferon regulatory factor (IRF)-3 with the
property of the viral autoprotease protein Npro. Reportedly, the amino acid
residues in the zinc-binding TRASH motif of Npro determine the difference in
characteristics between END-phenomenon-positive (END+) and
END-phenomenon-negative (END−) classical swine fever viruses (CSFVs). However,
the basic mechanism underlying this function in bovine viral diarrhea virus (BVDV) has not
been elucidated from the genomic differences between END+ and END−
viruses using reverse genetics till date. In the present study, comparison of complete
genome sequences of a pair of END+ and END− viruses isolated from
the same virus stock revealed that there were only four amino acid substitutions (D136G,
I2623V, D3148G and D3502Y) between two viruses. Based on these differences, viruses with
and without mutations at these positions were generated using reverse genetics. The END
assay, measurements of induced type I interferon and IRF-3 detection in cells infected
with these viruses revealed that the aspartic acid at position 136 in the zinc-binding
TRASH motif of Npro was required to inhibit the production of type I interferon
via the degradation of cellular IRF-3, consistently with CSFV. 相似文献
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