贵州省2008年油菜区试材料的SSR遗传多样性分析

为揭示油菜区试材料的亲缘关系,利用SSR分子标记技术分析了25份参加2007—2008年贵州省油菜区试材料的遗传多样性。结果表明:扩增出的115条带中45条呈现多态,多态性比率为39%。25份材料间的遗传相似系数在0.43~0.95,平均为0.69。在遗传相似系数0.63处分为3个类群,同一单位育出的材料往往优先聚为一类,遗传背景相近。     

AFLP与SSR标记及其在油菜研究中的应用

AFLP(扩增片段多态性)标记和SSR(简单重复序列)是应用广泛的分子标记技术,从遗传多样性、品种鉴定与指纹图谱构建、遗传连锁图谱构建、基因定位及分子标记辅助育种等方面概述了这2种标记在油菜上的应用,并对其应用前景进行了展望.     

Effect of shading colours on growth and pigment content of Gracilaria fisheri (Xia & Abbott) Abbott,Zhang & Xia (Gracilariales,Rhodophyta)

Sporeling and tissue propagation of agarophytic seaweed, Gracilaria fisheri, aimed to investigate the optimal shading colour for the preservation. This study was conducted under different shading colours indoor and outdoor experiments. The indoor experiment: tissues and sporelings of the seaweed were cultured in plastic chambers under fluorescent lights and were wrapped with white, green, blue and red colour PVC sheets of light intensity of 20 ± 1 μmol m^?2 s^?1. The outdoor tissues were cultured in the plastic tanks under different shadings of Saran sheets: white, green, blue and black covering the hoop‐houses with light intensity of 110 ± 10 μmol m^?2 s^?1. The results showed that different shading colours strongly influenced growth of G. fisheri sporelings and tissues but little effect on pigmentation. After 40 days, the highest growth rates of G. fisheri tissues and sporelings were found under red light with 1.44 and 1.89% day^?1 respectively. There was no significant difference (P > 0.05) on pigment content of tissues indoor, whereas it was found that chlorophyll concentration of the sporelings under green light with 416.8 μg g^?1 fresh weight was significantly higher (P < 0.05) than those in other treatments. After 8‐week culture, outdoor tissues showed the highest growth rate with 1.71% day^?1 in the green hoop‐house. The study has found that G. fisheri cultivation was less influenced by epiphytes in the back hoop‐house with 15%. This study showed the feasibility of successful cultivation of G. fisheri sporelings and tissues.