黑白花奶牛白细胞介素-2基因的克隆和表达(英文)

[Objective] The aim of this study is to clone bovine interleukin-2 gene(IL-2)and observe its expression in prokaryotic cells.[Method]Bovine IL-2 gene was amplified from total RNA of peripheral blood lymphocytes of holstein-friesian cows by RT-PCR.Subsequently,the gene was cloned into pGEX-2T prokaryotic expression plasmid to construct recombinant,which was then transformed into Escherichia Coli BL21.After IPTG induction,SDS-PAGE analysis was conducted.[Result]A 500 bp target fragment corresponding with expectation was obtained by RT-PCR.The cloned gene successfully expressed fusion protein of about 43 kD in prokaryotic cells.[Conclusion]This study provided a theoretical and material basis for further researches on IL-2 gene.     

牛白细胞介素-2基因的克隆与序列分析

白细胞介素-2(interleukin-2,IL-2)是一种具有广泛生物学活性的细胞因子,主要由激活的T淋巴细胞产生,它能提高免疫细胞杀伤癌细胞、病毒或细菌感染细胞以及促进抗体生成和分泌的能力[1-2],在机体正常免疫过程中起着十分重要的作用.     

黑白花奶牛白细胞介素-2基因的克隆和表达

[目的]克隆牛白细胞介素-2基因(IL-2),并观察其在原核细胞中的表达。[方法]应用RT-PCR技术从黑白花奶牛外周血淋巴细胞总RNA中扩增编码牛IL-2基因,并将其克隆到pGEX-2T原核表达质粒中,转化大肠杆菌BL21,经IPTG诱导后进行SDS-PAGE分析。[结果]通过RT-PCR扩增获得500 bp的目的片段,所克隆的IL-2基因在原核细胞中成功表达,表达产物为分子量约43 kD的融合蛋白。[结论]该研究为IL-2基因的进一步研究提供了理论依据和物质基础。