土壤中烟草根黑腐病菌的实时定量PCR检测技术研究

 Thielaviopsis basicola is a soil-borne plant pathogen which causes root rot disease in tobacco plants. Detection and monitoring of T. basicolain soil is of great significance to control this disease. Based on the differences in internal transcribed spacer (ITS) sequences of T. basicola and other fungal pathogens, a specific primer pair Tb1/Tb2 for T. basicolawas developed. The results showed that the primer pair gave a single amplicon of 330 bp from T. basicola and revealed no undesirable cross-reaction with other seven soil-borne pathogen isolates and three tobacco rhizosphere dominant fungi isolates. With a series of 10-fold genomic DNA dilutions of T. basicola, the detection limit of 1 pg/μL in conventional PCRand100 fg/μL in real-time quantitative PCR was achieved. With DNA from the soil inoculated with different numbers of T. basicola conidia, the detection limit was 10 conidia per reaction in conventional PCR and 0.4 conidia per reaction in real-time quantitative PCR.     

高粱单宁的研究进展综述

高粱是世界上广泛种植的主要粮食之一,其中丹宁是影响高粱精深加工的重要因素。高粱丹宁作为膳食用时,会降低摄食率和营养物质的利用率。而在生物系统中,具有收敛、止血和抗氧化作用。本文就高粱丹宁的结构、种类、对高粱的影响及其检测和去除方法进行综述,以期为高粱丹宁的研究做出参考。     

高粱镉胁迫响应基因sbbHLH168的表达特征及生物信息学分析

[目的]以高梁基因sbbHLH168为研究对象，探究其耐镉胁迫发热分子机制，对培育耐镉高粱提供了理论参考。[方法]从高粱品种“BTx623”镉胁迫下转录组数据(RNA-seq)中筛选出一个受镉胁迫后显著上调表达的基因sbbHLH168,使用在线软件对其生物信息进行分析；通过洋葱表皮侵染进行亚细胞定位；构建pGBKT7-sbbHLH168载体验证sbbHLH168基因的转录激活活性；将水培至四叶一心的高粱置于NaCl、PEG、ABA、ACC、GA和JA溶液处理，分别在0、1、12、24和48 h提取RNA,通过RT-qPCR检测sbbHLH168基因的相对表达量。[结果]sbbHLH168基因的开放阅读框长度为567 bp,编码189个氨基酸残基，蛋白分子量为20.55 kD,理论等电点(PI)为8.86,为碱性带正电的蛋白，定位于细胞核，具有较强的转录激活活性。sbbHLH168基因在盐、干旱和植物激素胁迫下表达量整体呈上升趋势，但表达量在不同处理时间存在明显差异。对sbbHLH168基因在不同组织中的表达发现，该基因在高粱的根和叶中均有表达，其中在盐、干旱、ABA、GA和JA胁迫下，...