A framework for evaluation of on-farm mastitis diagnostics in Australia

Numerous culture-based diagnostics are available on the Australian and international markets for on-farm detection of bacterial pathogens in milk. Use of such diagnostics may provide an opportunity to improve the prudent use of antimicrobials in udder health management. Farms are low-resource settings in terms of diagnostic microbiology capacity. The World Health Organisation has identified criteria for the evaluation of diagnostic tests in low resource settings based on Accuracy, Sensitivity, Specificity, User-friendliness, being Rapid or Robust, Equipment-free and being Deliverable (ASSURED). Here, we review how those criteria can be interpreted in the context of microbiological diagnosis of mastitis pathogens, and how on-farm diagnostics that are currently available in Australia perform relative to ASSURED criteria. This evaluation identifies multiple trade-offs, both with regard to scientific criteria and with regards to convenience criteria. More importantly, the purpose of testing may differ between farms, and test performance should be evaluated relative to its intended use. The ability of on-farm mastitis diagnostics to inform mastitis treatment decision-making in a timely and cost-effective manner depends not just on test characteristics but also on farm-specific pathogen prevalence, and on the farm enterprise's priorities and the farm manager's potential courses of action. With most assay evaluations to date conducted in professional laboratories, there is a surprising dearth of information on how well any of the diagnostic tests perform on-farm and, indeed, of the on-farm decision-making processes that they aim to inform.     

Comparative Expression Analysis of Gametogenesis‐Associated Genes in Foetal and Adult Bubaline (Bubalus bubalis) Ovaries and Testes

This study was conducted to identify and analyse the expression of gametogenesis‐associated genes and proteins in foetal and adult buffalo gonads of both the sexes. Relative quantification of the genes was determined by qPCR and Western blotting. Immunohistochemistry was also performed for various gametogenesis‐associated proteins in foetal and adult gonads of both the sexes. We observed significantly (p < 0.05) increased expression of primordial germ cell‐specific, meiotic as well as genes associated with oocyte maturation and development in foetal ovaries as compared to the adult ones. However, significantly (p < 0.05) increased expression of proteins associated with oocyte maturation like GDF9 and ZP4 was found in adult ovaries, indicating temporal regulation of mRNA translation during oogenesis. Meiotic genes showed significantly (p < 0.05) increased expression in adult testes as compared to foetal testes and ovaries, indicating onset of meiosis at a later stage in spermatogenesis. In general, the expression of primordial germ cell‐associated as well as meiotic genes was higher in adult testes, indicating the increased biological activity in the organ. Immunohistochemistry revealed localized expression of gametogenesis‐associated proteins in ovarian follicles and seminiferous tubules of testes, while the surrounding somatic tissues were devoid of these proteins. The study gives an understanding of the sequential and temporal events of gene expression as well as mRNA translation during male and female gametogenesis. It could also be concluded that follicles and seminiferous tubules are the functional units of the female and male gonads, respectively, and their function could be enhanced by appropriate chemical and genetic intervention of the somatic tissue immediately surrounding them. This assumes importance in the context that buffalo attains sexual maturity at an older age of 2–3 years and have smaller ovaries with lesser number of primordial follicles in comparison with cattle, which is suggested to be the main reason of their poor breeding performance.     

Projecting the bird community response resulting from the adoption of shelterbelt agroforestry practices in Eastern Nebraska

Evolving agricultural policies have influenced management practices within agroecosystems, impacting available habitats for many species of wildlife. Enhancing wildlife habitat has become an explicit objective of existing agricultural policy. Thus, there is renewed focus on field borders and the use of shelterbelt agroforestry systems to achieve conservation goals in the Midwest. Two Representative Farms – a 283-ha dryland and 510-ha irrigated farm were created in Saunders County, Nebraska. The Habitat Analysis and Modeling System (HAMS) was used to describe the composition and spatial pattern of the existing farms and surrounding landscape, as well as for the landscapes surrounding selected Breeding Bird Survey (BBS) routes. Simulated land use changes resulting from the implementation of two shelterbelt scenarios, Agricultural and Wildlife, were incorporated on each Representative Farm and surrounding landscape. Landscape variables which influence breeding bird species richness and community composition as determined from BBS routes were measured on simulated farm landscapes. A more heterogeneous landscape results from implementing either scenario. The percent total woods was a significant determinant of bird species richness on the BBS routes and was important in influencing bird communities at the farm- and landscape-level. Other landscape metrics which influenced the bird community composition on BBS routes were woody edge percentages and edge density values. Policies promoting shelterbelts create edge habitats which ultimately favor birds within the Forest-edge/generalist guild while bird species in need of conservation such as grassland-field species would potentially be negatively affected. This revised version was published online in June 2006 with corrections to the Cover Date.     

Creep in chipboard

Summary A considerable amount of data has been collected over the past few years on the rupture life of particleboard. This information is particularly valuable at the present time because there is increasing interest in the use of particleboard as a structural material. This paper presents a conventional analysis of the time to failure of three commercially available brands of particleboard at 20°C and at three fixed levels of relative humidity. A linear relationship is assumed between the logarithm of time to failure and stress level, and an improvement is made in the usual method of analysis by including censored specimens. The regression lines are projected forward to give estimates of the stress levels that can be sustained for a particular design life. It is found that the regression lines for 30 per cent and 65 per cent rh may be combined, but the specimens at 90 per cent rh are significantly lower in strength at any given time. One of the MUF bonded boards performs significantly better than a second MUF board over the long term, and this gives added weight to the view that there are other factors in the make-up and production of the board that are at least as important as the resin type itself.     

Bioaccumulation of Lead, Calcium and Strontium and their Relationships in the Octopus Octopus vulgaris

Octopuses (Octopus vulgaris) landed from commercial fishing were sampled and the concentrations of lead, calcium and strontium, in digestive gland, branchial heart, gills, mantle and arms, were determined using PIXE (particle-induced X-ray emission). Lead was detected in all tissues, although not in samples from all animals analysed. Female octopuses accumulated more lead in digestive gland than did males. The digestive gland index, an indicator of nutritional status, showed a negative correlation with lead concentration in both sexes. There were positive correlations between the concentration of lead and those of calcium and strontium in digestive gland and a negative correlation between lead and calcium in branchial heart tissue. Concentrations of calcium in arms were lower in autumn and spring than in winter and summer. Lead content increased with increasing body weight and mantle length so we can conclude that lead continues to accumulate during the animal’s life. Concentrations of lead in two samples were higher than the maximum legally permitted concentration of lead in food.     

Hydrogen ion input to the hubbard brook experimental forest,New Hampshire,during the last decade

Being downwind of eastern and midwestern industrial centers, the Hubbard Brook Experimental Forest offers a prime location to monitor long-term trends in atmospheric chemistry. Continuous measurements of precipitation chemistry during the last 10 yr provide a measure of recent changes in precipitation inputs of H ion. The weighted average pH of precipitation during 1964–65 to 1973–74 was 4.14, with a minimum annual value of 4.03 in 1970–71 and a maximum annual value of 4.21 in 1973–74. The sum of all cations except H ion decreased from 51 μeq 1^?1 in 1964–65 to 23 μeq 1^?1 in 1973–74 providing a significant drop in neutralizing capacity during this period. Based upon regression analysis, the input in equivalents of H ion and nitrate increased by 1.4-fold and 2.3-fold respectively, from 1964–65 to 1973–74. Input of all other ions either decreased or showed no trend. Based upon a stoichiometric formation process in which a sea-salt, anionic component is subtracted from the total anions in precipitation, SO₄ ⁼, contribution to acidity dropped from 83% to 66%, whereas NO₃ ^? increased from 15% to 30% during 1964–65 to 1973–74. The increased annual input of H ion at Hubbard Brook during the past 10 yr is highly correlated with the increased input of nitrate in precipitation.