采后黄瓜在冷驯化处理过程中的转录组变化

采后黄瓜是冷敏性果菜类蔬菜，在低温贮藏时易发生冷害。前期研究结果表明，冷驯化处理通过诱导采后黄瓜耐冷性，减少冷害发生。为探究冷驯化处理诱导的转录组学变化，以采后黄瓜为试材，分析冷驯化处理期间的转录组变化。与贮藏前（0 h）相比，在冷驯化处理12 h和72 h时，分别鉴定到1 870和3 550个差异表达基因。基因表达验证结果表明，RT-qPCR和转录组结果高度一致，证明转录组测序数据的准确性和可靠性。GO富集分析结果显示，冷驯化处理诱导的差异表达基因主要富集在氧化还原过程、细胞膜组分和转录因子活性3个GO途径中，表明冷驯化处理通过调节细胞膜组分、细胞内氧化还原状态，增强冷藏黄瓜耐冷性。进一步分析发现，104个转录因子基因响应冷驯化低温，差异表达的转录因子主要是ERF、bZIP、WRKY和HSF家族，表明转录因子介导的转录调控在冷驯化诱导的耐冷性中发挥重要作用。研究结果为采后黄瓜诱导耐冷性提供了新见解，有助于加深对冷驯化诱导耐冷性分子机理的认识，为耐冷黄瓜培育提供了重要基因资源。     

Feasibility of soil washing agents to remove fluoride and risk assessment of fluoride-contaminated soils

Journal of Soils and Sediments - The objectives of this study are to find the most suitable soil washing agent and the optimal concentration for fluoride removal and to further provide insights...     

油料作物种子中神经酸含量提升研究进展

神经酸（NA）是一种超长链单不饱和脂肪酸,为大脑神经纤维髓鞘的核心成分,具有修复受损大脑神经纤维、促进神经细胞再生等功能。因此,NA受到越来越多的研究和关注。目前NA主要依赖于从天然物种中提取,难以满足市场需要,限制其进一步发展和应用。近年来,随着基因工程以及合成生物学的发展,通过多基因策略,在现有物种中构建NA代谢路径,可经济且可持续地获得NA,以满足营养、制药和化学工业等领域的需求。本文综述了NA的生物功能、生物来源、合成途径,并比较了基因工程生产NA各底盘的优劣,进一步探讨了十字花科油料作物底盘生产富含NA种子油的影响因素,以期为将来通过转基因技术和分子育种调控NA的生产,获得可再生的NA资源提供一定的思路和策略。     

花培晚粳890的农艺性状及其栽培技术研究

应用细胞工程技术培育而成的晚粳新品系８９０，通过五年的多点试验、示范种植，结果显示：熟期适中，高产、优质，适应性强。总结出适时播种、培育壮秧、合理密植、科学施肥、加强水浆管理等高产栽培技术，为该稻在沿江和江南地区种植提供科学依据。     

Observations on soil and groundwater contamination with nitrate: A case study from Lebanon-East Mediterranean

The impact of agricultural practices on soil-groundwater quality in the sub-humid Bekaa plain of Lebanon-East Mediterranean was monitored in four fields (F) between July 2007 and July 2009. These were occupied by continuous mint (F1), summer potato/wheat/potato (F2), lettuce/lettuce/potato/wheat/summer potato (F3) and table grapes (F4). N input calculated on a two-year basis, was in the following ascending order F4, F2, F3 and F1. Soil samples, analyzed down to 200 cm depth, showed high nitrate and chloride concentrations at the end of the 2007 and 2008 seasons. Soil chloride and nitrate peaks recorded in October 2007 and 2008 disappeared below 200 cm overwinter. The calculated N biannual discharge ranged from 130 (F4), to 516 (F2), to 778 (F1), to 879 kg ha⁻¹ (F3). Groundwater quality was studied in 21 wells distributed along a sequence stretching from the Litani River to the eastern water dividing line. Based on the nitrate concentrations, the well located at the top of the water dividing line was the only one suitable for drinking purposes. Eight wells were mildly contaminated, therefore suitable for irrigation purposes except for sensitive crops. Twelve wells, positioned in the plain, showed a nitrate level exceeding 200 mg L⁻¹. Protecting the soil and groundwater quality is a top priority to maintain the ecological and agricultural functions of water.     

Evaluation of the effects of sweet potato (Ipomoea batatas (L.) Lam) in broiler diets

Cereal grains such as maize and wheat are used extensively in feed formulations for poultry as the primary source of carbohydrates. High cost of these grains in many developing countries necessitates the evaluation of other ingredients that are grown locally. Sweet potato is one such crop. The study was conducted as a proof of concept experiment to test the hypothesis that in the presence and absence of enzyme, sweet potato roots when included in diets of broiler chickens may affect the total metabolisable energy content of the diets which may exert certain influences on dry matter digestibility of these diets as well as impacting on production and certain gut parameters. A total of 120 chicks were raised on a commercial starter feed from day 0 to 19. On day 22, the birds were individually weighed and allocated to 96 single bird metabolism cages to conduct a 7‐day classical apparent metabolisable energy (AME) assay. The test diets contained 0% and 25% sweet potato flour (SPF) with and without enzyme supplementation (Rovabio Excel AP T‐flex) and replicated 24 times. AME of the control diet with and without enzyme was 14.05 and 13.91 MJ/kg whilst the AME of the SPF diets with and without enzymes were 13.45 and 13.43 MJ/kg respectively. AME of SPF was 12.08 MJ/kg. Birds fed the SPF had significantly reduced end weights (p = .002) and weight gains (p < .001) leading to significantly higher intake (p = .004) and FCRs (p < .001) in birds. These effects in growth parameters highlight the need to balance dietary protein and total amino acids when using SPF in broiler diets and may not be a negative effect of SPF per say as AME and dry matter digestibility of SPF diets were comparable to the control diet. The level of sucrase activity in the jejunum was significantly (p < .001) lower due to enzyme inclusion. Use of SPF in the current study did not negatively influence the activities of the brush border enzymes maltase and sucrase, gut morphology in the jejunum of broilers or the load of Enterobacteriaceae in the caecal of birds. This finding is promising in that the gut parameters associated with digestive capacity and gut health were not compromised with feeding of SPF to broilers.     

LAND RESOURCES OF THE MEDITERRANEAN: STATUS,PRESSURES, TRENDS AND IMPACTS ON FUTURE REGIONAL DEVELOPMENT

The Mediterranean region covers about 854 million ha, but only 118 million (or 14 per cent) are suitable for agricultural production. In North Africa and the Middle East (MENA), agricultural land covers about 5 per cent; in Egypt and Algeria, it occupies less than 4 per cent and, in Libya, less than 2 per cent of the total national land area. Across the Mediterranean region land use divides between natural pastures/rangelands (ca. 15 per cent), forests and woodlands (ca. 8 per cent), with the ca. 63 per cent remaining desert sands, shallow, rocky, saline, sodic soils and areas effectively sealed by urbanisation. Land degradation is a severe problem in most Mediterranean countries. Estimates for the period 1961 to 2020 show that although the Mediterranean population is likely to more than double, ca. 8.3 million ha of agricultural land (7 per cent) may be lost as a result of continued urbanisation and land degradation. If these estimates are correct, agricultural land per capita would more than halve from ca. 0.48 ha (1961) to ca. 0.21 ha in 2020. Food security is likely to become increasingly problematic, especially in the MENA countries, which require a major reassessment of their agricultural development policy. Copyright © 2012 John Wiley & Sons, Ltd.     

组培中粳8715特征特性及高产栽培技术

早熟中粳８７１５，采用生物工程技术，选取当选晚２号适宜时期的健壮幼穗，进行体细胞培养，再生稻株，经多次田间试验、示范种植选育而成。该稻性状稳定，株形理想，生长青秀，光合效率高。根系健壮发达、不早衰。穗大粒多，熟期整齐，成熟时秆青籽黄，熟相佳。品质优，综合抗性强。全生育期１３３天。亩产４５０ｋｇ以上。适宜沿淮及淮北和江淮之间种植。     

Land use Change,a Case Study from Southern Italy: General Implications for Agricultural Subsidy Policies

For many years, farmers across Italy have struggled to convert rocky landforms into farmlands. Recently, expensive, heavy machineries have been widely used for these activities, exaggerating costs on land preparation. Subsidised by the Italian authorities, nearly 20% of Apulia lands in southern Italy have been converted to farmland through these methods. Recent evidence shows that in the short term, rock grinding enriches the soil with nutrients and improves soil quality. However, over the long run, land degradation and ecosystem service deterioration become prominent, resulting in the abandonment of extensive lands. This Italian case study should be considered as an alarming example of the possible detrimental effects of the national subsidies policy that supports the conversion of natural landforms into agricultural lands. Copyright © 2015 John Wiley & Sons, Ltd.