Growth characteristic of Camel pox and Vaccinia viruses in embryonated eggs and cell culture

Camel pox viruses isolated in Sudan and VD45 (African camel pox strain) and Vaccinia virus (Elstree strain) were used for inoculation of chorioallantoic membrane (CAM) of embryonated eggs (EE) and cell culture (CC). In EE Lesions were seen as pocks ranging in size from 1 to 1.5 mm in diameter, and they increase in size with serial passage and taking opaque- white and opaque- yellow colors. When propagated in Vero cells, these viruses gave clear CPE, characterized by rounding of cells, plaque formation, syncytia and detachment of cells from the glass.     

蓖麻种子吸水和萌发过程对盐分胁迫条件的响应

为确定盐分对蓖麻的影响,设置0 mM和100 mM 2个盐分浓度NaCl,研究了4个蓖麻品种种子的吸水和萌发特性.在吸水开始的18h内,种子吸水快速增加,18 h后增加缓慢,48 h后在0 mM NaCl水平下种子吸水呈快速增加趋势,而在100 mM NaCl水平下吸水很少.汾蓖10号和淄蓖7号的吸水量和吸水速率显著高于淄蓖5号和淄蓖8号.与0 mM NaCl相比,同一品种在100 mM NaCl下的吸水量和吸水速率明显较低.100mM NaCl处理对种子萌发具有明显的抑制作用,表现为延迟种子萌发,降低发芽率,抑制芽长和芽粗.综合发芽率、芽长和芽粗相对盐害率,淄蓖5号耐盐能力最强,其次是汾蓖10号,淄蓖7号和淄蓖8号耐盐能力相对较差.本试验通过研究不同蓖麻品种的吸水和萌发特性,为采用外源措施提高种子吸水能力,促进种子萌发和出苗奠定基础.     

Detection of camel pox and vaccinia viruses by polymerase chain reaction

PCR following two methods of DNA extraction was used to confirm the growth of camel pox virus (CPV) and vaccinia virus in cell culture and chorioallantoic membrane. Results were compared with the commonly used neutralization test. The first method of DNA extraction was accomplished by using viral DNA in tissue culture supernatant and Chorioallantoic membrane, which was released by initial heating for 15 min at 99°C followed by ordinary PCR. In the second method DNA was extracted by using DNA Isolation Kit from tissue culture supernatant and used as a template. Rapid identification and differentiation of CPV and Vaccinia virus were achieved by PCR and this assay proved to be fast and feasible, and can be an alternative to orthodox serological methods.