Detection of camel pox and vaccinia viruses by polymerase chain reaction |
| |
Authors: | Hanan M Sheikh Ali A I Khalafalla A H Nimir |
| |
Institution: | (1) Department of Microbiology, College of Veterinary Medicine & Animal Production, Sudan University of Science &Technology, P.O. Box: 204, Khartoum North, Sudan;(2) Department of Microbiology, Faculty of Veterinary Medicine, University of Khartoum, Shambat, 13311 Khartoum North, Sudan |
| |
Abstract: | PCR following two methods of DNA extraction was used to confirm the growth of camel pox virus (CPV) and vaccinia virus in
cell culture and chorioallantoic membrane. Results were compared with the commonly used neutralization test. The first method
of DNA extraction was accomplished by using viral DNA in tissue culture supernatant and Chorioallantoic membrane, which was
released by initial heating for 15 min at 99°C followed by ordinary PCR. In the second method DNA was extracted by using DNA
Isolation Kit from tissue culture supernatant and used as a template. Rapid identification and differentiation of CPV and
Vaccinia virus were achieved by PCR and this assay proved to be fast and feasible, and can be an alternative to orthodox serological
methods. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|