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排序方式: 共有75条查询结果,搜索用时 15 毫秒
1.
Dhungana Sanjeev Kumar Kim Il-Doo Adhikari Bishnu Kim Jeong-Ho Shin Dong-Hyun 《Journal of Crop Science and Biotechnology》2019,22(1):11-16
Journal of Crop Science and Biotechnology - Due to the negative consequences of synthetic herbicides use and their reducing effectiveness due to development of resistant weeds, promotion of... 相似文献
2.
Richa Sood Rekha Khandia Sandeep Bhatia Divakar Hemadri Manoj Kumar Sharan S. Patil Atul K. Pateriya Arshi Siddiqui Malkanna Sanjeev Kumar Mudalagiri Dasappa Venkatesha Diwakar D. Kulkarni 《Tropical animal health and production》2014,46(6):1037-1043
Malignant catarrhal fever (MCF) is a fatal herpesvirus infection of domestic and wild ruminants, with a short and dramatic clinical course characterized primarily by high fever, severe depression, swollen lymph nodes, salivation, diarrhea, dermatitis, neurological disorders, and ocular lesions often leading to blindness. In the present study, fatal clinical cases of sheep associated malignant catarrhal fever (SA-MCF) were identified in cattle in the state of Karnataka. These cases were initially presented with symptoms of diarrhea, respiratory distress, conjunctivitis, and nasal discharges. Laboratory diagnosis confirmed the detection of ovine herpesvirus-2 (OvHV-2) genome in the peripheral blood samples of two ailing animals. The blood samples collected subsequently from sheep of the neighboring areas also showed presence of OvHV-2 genome indicating a nidus of infection in the region. The positive test results were further confirmed by nucleotide sequencing of the OIE approved portion of tegument gene as well as complete ORF8 region of the OvHV-2 genome. Phylogenetic analysis based on the sequence of the latter region indicated close genetic relationship with other OvHV-2 reported elsewhere in the world. 相似文献
3.
The univoltine European corn borer (ECB) has become a major limiting factor for maize (Zea mays L.) production in central Europe. The objective of this study was to survey the genetic variation for ECB resistance in European elite maize germplasm. Eighteen flint and 23 dent inbreds were screened under artificial ECB infestation at two locations in 1993 and 1994. Resistance was assessed by damage rating of broken plants, measurement of tunnel length in dissected stalks, and yield reduction in infested plots relative to insecticide-protected control plots. Flint lines showed significantly greater means for damage rating than dent lines with grain yield reduction of 35% and 24%, respectively. Significant genotypic variances among lines and high heritabilities were found for agronomic traits and damage rating. Heritabilities were intermediate for tunnel length and relative grain yield. Significant associations of days to silking, ear dry matter content, and dry matter yield of the whole plant with damage rating and tunnel length suggested a better resistance in late-maturing, high-yielding inbreds. Genotypic correlations of relative grain yield with tunnel length and damage rating ranged between ?0.46 and ?0.72. Partial correlations, eliminating the effect of flowering time, confirmed these associations. Damage rating of stalks is the most suitable trait for evaluation of ECB damage owing to its high heritability and easy recording. Tunnel length below the primary ear is a useful trait for assessing antibiosis because it is not correlated with days to silking. Inbreds with extreme resistance and susceptibility were identified which can be used as parents for establishing breeding and QTL mapping populations. 相似文献
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Aditya Pratap Umashanker Prajapati Chandra Mohan Singh Sanjeev Gupta Meenal Rathore Nupur Malviya Rakhi Tomar Ajeet Kumar Gupta Swapnila Tripathi Narendra Pratap Singh 《Plant Breeding》2018,137(3):235-249
Grain legumes, the important constituents of sustainability‐based cropping systems and energy‐limited vegetarian diets have long been the subject of scientific research. Tremendous technological strides were made in the so‐called orphan crops, in terms of both varietal improvement and generation of basic information. Despite recalcitrancy and high genotype dependency, in vitro culture techniques such as organogenesis, in vitro mutagenesis, embryo rescue and in vitro gene transfer have been deployed for improvement of several grain legumes and these played an important role in introgression of desirable genes from related and distant species and creation of additional genetic variability. Stable and reproducible regeneration protocols resulted in the development of genetically modified chickpea, pigeon pea, cowpea, mungbean, etc., while embryo rescue was deployed successfully for recovery of interspecific recombinants, a few of them exploited for the development of commercial cultivars. Nevertheless, doubled haploidy witnessed limited success and protoplast regeneration and in vitro mutagenesis remained of academic interest. The present review focuses on the progress, achievements, constraints and perspectives of using in vitro technology in grain legume improvement. 相似文献
7.
Arjunan Jeevalatha Priyanka Kaundal Ravinder Kumar Baswaraj Raigond Rakesh Kumar Sanjeev Sharma Swarup Kumar Chakrabarti 《European journal of plant pathology / European Foundation for Plant Pathology》2018,150(3):565-573
Apical leaf curl disease of potato is caused by a whitefly transmitted begomovirus, Tomato leaf curl New Delhi virus-[potato] (ToLCNDV-[potato]) in India. Detection of this virus is essential to manage the disease, particularly in healthy potato seed production systems. Large scale testing of micro-plants demands a simple, rapid and sensitive assay. Hence, loop-mediated isothermal amplification (LAMP) method was developed for specific detection of ToLCNDV-[potato]. Six primers that recognize the coat protein gene sequence of ToLCNDV-[potato] were designed and LAMP assay was optimized using different concentrations of magnesium sulphate, betaine, dNTPs, Bst DNA polymerase and temperature. The results were assessed by visual observation of turbidity, colour change using SYBR green dye and also by gel electrophoresis. The assay successfully detected the virus in infected plants collected from potato fields whereas no cross-reactions were observed with healthy plants and other potato viruses. The optimized assay was as sensitive as PCR assay and could detect up to 0.002 pg of total DNA. The assay could detect the virus in infected potato tubers and also in asymptomatic plants. Print-capture LAMP assay was developed and its application could reduce the cost and time of the assay in large scale testing under seed production. 相似文献
8.
Baswaraj Raigond Ambika Verma Tarvinder Kochhar Shivani Roach Sanjeev Sharma S. K. Chakrabarti 《Phytoparasitica》2018,46(2):255-262
A simple, cost-effective and rapid viral nucleic acid release (NAR) buffer suitable for RT-PCR based diagnostic assay was developed for the detection of potato viruses. The NAR buffer and commercially available RNA isolation kit were compared for RT-PCR based assay, where an amplicon of expected size (~380 bp) targeting PVY was observed in both isolations indicating that it can be used in RT-PCR based diagnostic assays. The same was further validated for its repeatability by running across more than hundred suspected potato leaf samples collected from different sources where, it showed consistent results for the presence of PVY indicating its reliability. The NAR buffer assay was examined for its sensitivity in comparison with the kit based isolation where both the assays were able to detect even up to 10?5 dilution without affecting the sensitivity. NAR buffer was found stable up to 28 days at -20 °C and for 14 days at 4 °C without losing PCR sensitivity. The assay was also found effective to release the nucleic acid from potato leaves, thrips and aphids for PCR and RT-PCR based detection of DNA viruses like ToLCNDV-potato and other RNA viruses. The developed protocol is simple, less laborious, time-saving (10-15 min) and economical (1/100th of kit) as compared to kit based protocol. The assay can be adopted in diagnostic laboratories for detection of RNA/DNA viruses from potato plants and in thrips as well. 相似文献
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J. C. Rana K. S. Negi S. A. Wani Sanjeev Saxena K. Pradheep Anjali Kak S. K. Pareek P. A. Sofi 《Genetic Resources and Crop Evolution》2009,56(7):963-973
The Western Himalayan region of India possesses rich genetic diversity of rice (Oryza sativa L.). Rice landraces having withstood the rigors of biotic and abiotic stresses, suit to the local conditions of farms and
reflect socio-cultural preferences can still be found in crop fields located distantly in rural and tribal areas. This region
is known for growing World fame basmati in the foot hills and many varieties of red rices grown at higher elevations, known
for cold tolerance and medicinal properties. Genetic resources of rice were collected for about 8 years (1999–2006) and 1069
germplasm accessions including 154 named landraces were collected. Prominent landraces such as Begumi, Ramjwain, Thapachini, Naurang, Hansraj, Tilakchandan, Lalsati, Jhini, Mushakbudji, Jattoo, Barpasso, Qadirbeigh, Safedbrez,
Shahie known for their special quality attributes were collected. The change in land use, cropping patterns and aggressive introduction
of modern varieties in the region has resulted in the loss of a large number of landraces especially from irrigated lands.
The present paper looks at the status of rice genetic resources, genetic variability, genetic erosion and future strategies
to conserve rice germplasm on farm and to maximize its use in rice breeding. 相似文献