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An experiment was made to determine the absorption of purine metabolites in dietary nucleic acids through the digestive tract, and also to determine the utilization of nucleic acids absorbed in the body, using growing lambs. Two pairs of 120‐ and 180‐day‐old twin female lambs with a bodyweight of 18.2–19.0 kg were kept in metabolism crates and fed on purine‐free milk replacement (MR) with supplements of exogenous purine (purine base or purine nucleoside) at a level of 0.2 mmol/BW0.75/d for 5 consecutive days, and thereafter they were maintained in the crates for 4 days. The daily amount of exogenous purine supply was calculated based on the urinary excreted purine derivatives (PD) in lambs fed on milk replacement alone. A urine sample was collected daily for 9 consecutive days, and the urinary excretion of PD was determined daily. Urinary PD excretion opened to increase within 24 h after the dose of purine bases, and the level was recovered on 3 days after ceasing the exogenous purine supply. The recovery of PD in the urine was about 70% of the purine supplement. When purine nucleosides were added to the feed, urinary PD excretion was initiated within 24 h after dosing, and the values were recovered after ending the purine nucleoside supply. The recovery rate of PD in the urine was only 30% of the supplemented purine. The plasma allantoin levels were almost similar after feeding purine bases and purine nucleosides, and the values were mostly in the range (40–60 µmol/L). These findings indicate that an exogenous purine can be directly incorporated into the body, and the purine as nucleoside is more effectively utilized for the synthesis of nucleic acids than as a purine base in the body of growing lambs. 相似文献
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为了研究醋酸水溶液处理给杉树材重量带来的变化,使用醋酸水溶液对杉树材进行了处理。结果发现处理后杉树材重量的减小量大约等于抽出物的重量,而且随着醋酸水溶液浓度的增加而增加。利用傅立叶变换红外光谱法对处理后的杉树材及抽出物进行了光谱分析。从处理后杉树材的光谱图可以观察到3400cm-1附近的OH伸缩振动及1510cm-1附近的木质素中苯环C=C伸缩振动的吸收强度有增加倾向。从醋酸水溶液抽出物光谱图可以发现用浓度30%醋酸水溶液处理时木质素被溶出。图2表2参6。 相似文献
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Seung Hak YANG Tohru MATSUI Hiroyuki KAWACHI Tomoya YAMADA Naoto NAKANISHI Hideo YANO 《Animal Science Journal》2003,74(1):17-21
ABSTRACT This experiment was conducted to investigate leptin mRNA expression, adipocyte size, and their relationship in several adipose tissues of fattening steers. Subcutaneous, perirenal, intermuscular and intramuscular adipose tissues were collected from three crossbred steers (Japanese Black cattle X Holstein) aged 21 months. The mRNA level and adipocyte diameter were determined in these adipose tissues. The intramuscular adipose tissue had a lower leptin mRNA level than the intermuscular and perirenal adipose tissues (P < 0.05). Leptin mRNA level was lower in the subcutaneous depot than in the intermuscular depot (P < 0.05). Adipocyte diameter was larger in the intermuscular adipose tissue than in the subcutaneous and intramuscular adipose tissues (P < 0.05). Leptin mRNA level was positively correlated with adipocyte diameter (r2 = 0.81, P < 0.05). These results suggest that the cattle have fat depot‐specific differences in leptin gene expression, which are a result of a difference in adipocyte size. 相似文献
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Tomoaki ONODA Ryuta YAMAMOTO Kyohei SAWAMURA Harutaka MURASE Yasuo NAMBO Yoshinobu INOUE Akira MATSUI Takeshi MIYAKE Nobuhiro HIRAI 《Journal of Equine Science》2013,24(4):63-69
Percentile growth curves are often used as a clinical indicator to evaluate variations of
children’s growth status. In this study, we propose empirical percentile growth curves
using Z-scores adapted for Japanese Thoroughbred horses, with considerations of the
seasonal compensatory growth that is a typical characteristic of seasonal breeding
animals. We previously developed new growth curve equations for Japanese Thoroughbreds
adjusting for compensatory growth. Individual horses and residual effects were included as
random effects in the growth curve equation model and their variance components were
estimated. Based on the Z-scores of the estimated variance components, empirical
percentile growth curves were constructed. A total of 5,594 and 5,680 body weight and age
measurements of male and female Thoroughbreds, respectively, and 3,770 withers height and
age measurements were used in the analyses. The developed empirical percentile growth
curves using Z-scores are computationally feasible and useful for monitoring individual
growth parameters of body weight and withers height of young Thoroughbred horses,
especially during compensatory growth periods. 相似文献
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Daisuke FUKUI Masashi NAGANO Ryohei NAKAMURA Gen BANDO Shinichi NAKATA Masao KOSUGE Hideyuki SAKAMOTO Motozumi MATSUI Yojiro YANAGAWA Yoshiyuki TAKAHASHI 《The Journal of reproduction and development》2013,59(5):491-495
Artificial insemination (AI) can help to avoid inbreeding and genetic degeneration
for sustaining genetically healthy populations of endangered species in captivity.
Collection of a sufficient quantity of viable sperm is an essential first step in the
AI process. In the present study, we examined the effects of frequent
electroejaculation on semen characteristics in a Siberian tiger. We collected semen
in all 17 trials during 6 breeding seasons (6 years). The mean number of sperm and
the percentage of motile sperm were 294.3 ± 250.2×106/ejaculate and 82.4 ±
11.4%, respectively. The number of motile sperm tended to increase during frequent
electroejaculation in the same breeding season. Semen collection by
electroejaculation can be performed effectively up to the fourth sequential
ejaculate, which contained the most sperm in the study. In conclusion, frequent
collection of sperm by electroejaculation from tigers may be effective for collection
of a large number of motile sperm. 相似文献
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Kenichi MATSUI Takeshi OHTA Hisayo MORINAGA Tomohiko SASASE Sumiaki FUKUDA Makoto ITO Masatoshi UEDA Naoto OGAWA Katsuhiro MIYAJIMA Mutsuyoshi MATSUSHITA 《Animal Science Journal》2008,79(5):605-613
Spontaneously Diabetic Torii (SDT) fatty rats, made by introducing the fa allele of the Zucker fatty rat into the SDT rat genome, represent a new model of obese type 2 diabetes. SDT fatty fa/fa (SDT fatty) rats exhibit overt obesity, hyperglycemia, and hyperlipidemia from about six weeks of age, and this is associated with hyperphagia by an induced disorder of leptin action. The present study was conducted to elucidate whether suppression of hyperphagia can improve reduce abnormalities in SDT fatty rats. SDT fatty rats were subjected to pair-feeding with SDT fatty +/ + (SDT) rats from 6 to 26 weeks of age, and the effects on metabolic parameters and diabetic complications were assessed. Body weights of the pair-fed rats were similar with those of SDT rats during the experimental period. Improvement of hyperglycemia or hypertriglyceridemia was observed from 8 to 16 or 12 weeks of age in the pair-fed rats, but hypercholesterolemia was not entirely improved during the experimental period. We also examined mRNAs expression in liver, and found that the expression associated with glyconeogenesis, such as glucose-6-phosphatase (G-6-Pase) and phosphoenolpyruvate carboxykinase (PEPCK), tended to decrease in the pair-fed rats, and the mRNA expression of hydroxymethylglutaryl-CoA (HMG-CoA) was elevated. Renal parameters, such as blood urea nitrogen and urinary albumin excretion, were improved in the pair-fed rats. The incidence or progression of diabetic complications, such as renal lesions and cataract, was reduced. In conclusion, suppression of hyperphagia in SDT fatty rats was effective in temporally improving hyperglycemia or hypertriglyceridemia, and reducing the incidence or progression of diabetic complications, but was ineffective in reducing hypercholesterolemia. 相似文献
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