Effects of different lighting on reproductive system in depressive female rats

AIM: To investigate the effects of different lighting on the reproductive system in depressive female rats. METHODS: Healthy adult female rats were randomly chosen as control group, and the depressive adult female rats in SPF grade were randomly divided into 5 groups(7 rats each):depressive model group, sulfur lamp group, heat radiation lamp group, fluorescent lamp group and LED lamp group. After 45 d of continuous illumination, the estrous cycle was observed by the vaginal exfoliated cells, and the organ indexes of ovary and uterus were calculated. The concentrations of estiadrol(E₂), prolactin(PRL), progesterone(PROG) and testosterone(T) in the serum were detected by ELISA, and the histopathological lesion of ovary was observed under microscope with HE staining. RESULTS: The estrous cycle exhibited serious disorder, the ovaries exhibited serious congestion, and the organ indexes of ovary and uterus and the concentrations of E₂, PRL, PROG and T decreased significantly in the rats in depressive model group compared with control group(P<0.05). The estrous cycle and histopathological damage of ovary were obviously improved, and the concentrations of E₂, PRL, PROG and T were significantly increased after the sulfur lamp lighting in the depressive female rats compared with depressive model group. No obvious change and improvement of the reproductive functions in the heat radiation lamp group, fluorescent lamp group and LED lamp group was observed. CONCLUSION: The reproductive functions of depressive female rats are improved by sulfur lamp lighting.     

庐山应用引诱剂诱捕松墨天牛成虫试验

2009—2011年在江西省庐山,应用福建省林科院研制的FJ-MA-02引诱剂进行诱捕松墨天牛成虫试验。结果表明:松墨天牛成虫在庐山发生期为4月下旬末—9月上旬;随着海拔的升高,成虫羽化高峰期逐渐推迟,海拔220、550、800 m天牛羽化盛期分别在5月下旬、6月中下旬、7月中旬。通过清除松树枯死木、悬挂诱捕器、施放白僵菌等综合技术措施,3个试验点松树枯死木数量2011年与2009年同比下降了62.0%、60.8%、62.3%。说明引诱剂是监测和控制松墨天牛的有效措施之一。     

江西省萍乡市某羊场布鲁菌病的流行病学分析

江西省萍乡市某山羊场妊娠母羊散发流产,为了分析该羊场流产的发生是否与布鲁菌感染有关,同时掌握该羊场布鲁菌病的流行情况,随机抽样采血共分离得到100份山羊血清,首先使用虎红平板凝集试验进行初步鉴定,然后通过试管凝集试验重新检测阳性血清。结果表明,该羊场布鲁菌病阳性率为20％。由此可以得出,该羊场的布鲁菌病防控工作存在较严重的问题,应制定有效的防治措施,将山羊布鲁菌病的检测纳入日常疾病监测工作中,最终达到净化该病的目的。     

空间水稻精准栽植机构的建模与动态仿真

本文以变杆长空间连杆机构运动特性为研究基础;利用MATLAB软件对水稻精准栽植机构进行建模,规划其复杂运动轨迹,使其达到精准栽植要求;并利用SolidWorks三维软件实现了该机构运动轨迹的动态仿真,进一步证实该机构能够实现精准栽植目的。     

b-Boule基因5′调控序列的克隆与睾丸组织DMR甲基化分析

【目的】研究b-Boule基因5′调控序列的序列特征,以及牦牛、黄牛与犏牛睾丸组织b-Boule基因DMR甲基化状态的差异,为揭示b-Boule基因的表达调控和犏牛雄性不育的表观遗传机制提供依据。【方法】采用PCR扩增和克隆测序技术获得牦牛b-Boule基因5′调控序列,利用生物信息学方法分析b-Boule基因5′调控序列的序列特征,采用亚硫酸氢钠测序法检测牦牛、黄牛与犏牛睾丸组织中b-Boule基因DMR的甲基化状态。【结果】b-Boule基因5′调控序列长度为1 352 bp,核心启动子区含有SP1等甲基化敏感位点,5′端存在一个CpG岛。犏牛b-Boule基因DMR的甲基化水平(17.78%)高于牦牛(7.50%)和黄牛(6.94%)(P<0.01),特别是CpG位点33—35的甲基化水平差异更明显。【结论】犏牛b-Boule基因DMR的甲基化水平高于牦牛和黄牛,结合前期mRNA表达水平和组织学观察结果,认为DMR甲基化在b-Boule基因的表达调控中发挥关键作用,犏牛b-Boule基因可能是通过DMR区的高甲基化抑制其mRNA表达来阻滞精子发生减数分裂过程。     

犏牛及其亲本睾丸组织中印记基因SNRPN DMR甲基化与mRNA表达差异研究

【目的】研究犏牛与黄牛、牦牛睾丸组织SNRPN基因DMR甲基化状态、mRNA表达水平的差异,为揭示犏牛雄性不育的表观遗传机制提供依据。【方法】根据黄牛SNRPN基因序列设计引物,通过克隆测序获得牦牛SNRPN基因5'端序列,采用亚硫酸氢钠测序法检测犏牛及其亲本睾丸组织中SNRPN基因5'端DMR的甲基化状态,并采用Real-time PCR检测犏牛及其亲本睾丸组织中SNRPN基因的表达水平。【结果】牦牛SNRPN基因5'端序列长为1137bp,与黄牛的同源性达98.2%;生物信息学分析发现含有YY1和SP1等甲基化敏感位点。犏牛SNRPN基因DMR的甲基化水平(42.22%)极显著高于黄牛(21.08%)和牦牛(20.81%)(P0.01)。黄牛和牦牛睾丸组织中SNRPN基因mRNA表达水平高于犏牛,但未达到显著水平(P0.05)。【结论】犏牛睾丸组织SNRPN基因DMR的甲基化水平极显著高于黄牛和牦牛,且mRNA表达水平低于黄牛和牦牛,说明犏牛SNRPN基因可能是通过DMR区的高甲基化抑制其mRNA表达来阻滞精子发生减数分裂过程。     

冬夏两季畜禽废水的活性污泥中细菌遗传多态性研究

对冬夏两季膜生物器处理畜禽废水的效果进行研究,分析两季废水中细菌遗传多态性与废水处理效果的关系.直接提取废水中活性污泥总DNA,构建16S rDNA基因文库.选取两季各8个成功的阳性克隆子,分别采用Hinf Ⅰ、Hae Ⅲ、Alu Ⅰ、Msp Ⅰ 4种限制性内切酶消化重组质粒,电泳分别表现出不同谱型.结果表明,4种限制性酶中,Hinf Ⅰ的酶切图谱反应的遗传信息量最丰富.细菌遗传多态性Shannon-Wiener指数冬季为0.953,夏季为2.079,即夏季多态性高于冬季;根据出水水质监测,夏季处理效果优于冬季,与多态性指数呈正相关.